FastQCFastQC Report
Sat 18 Jun 2016
SRR3552342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278613
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71572.5687961437549576No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC9910.3556905097752079TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG9560.3431282818820371No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC8600.3086718853750543No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC8590.30831296457810653No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC8480.30436483581168144TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT5960.21391679498085156TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC3420.12275091255612625TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC2930.1051637935056871No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA351.2074997E-745.0000045
CGAACGG207.0240756E-445.0000042
CGTATGG207.0240756E-445.0000042
GCGCGCT207.0240756E-445.00000429
CACGACG207.0240756E-445.00000426
TGAAAGG551.8189894E-1245.0000042
ACCTACT207.0240756E-445.00000425
CGGATAC207.0240756E-445.00000414
ATAAGTG207.0240756E-445.00000437
GTCCGCA207.0240756E-445.00000423
CGGTCTA207.0240756E-445.00000431
ACGTAGG207.0240756E-445.0000042
CGGTATG302.1595624E-645.0000042
TCGACGG302.1595624E-645.0000042
TCGACAC207.0240756E-445.00000434
TATCGGG207.0240756E-445.0000043
TGCAACA207.0240756E-445.00000420
GGCAACG253.8831742E-545.01
ACGGGTC453.8198777E-1045.05
TGACCGG253.8831742E-545.02