##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552342_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278613
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.686870318326854	28.0	16.0	31.0	16.0	33.0
2	27.02429893795336	30.0	25.0	33.0	16.0	33.0
3	27.28328541740694	31.0	25.0	33.0	16.0	34.0
4	30.289020971742165	33.0	28.0	35.0	19.0	37.0
5	32.332001737176654	33.0	32.0	35.0	28.0	37.0
6	31.162522926065904	35.0	30.0	35.0	17.0	37.0
7	33.28901379332623	35.0	32.0	35.0	28.0	37.0
8	33.84548818612197	35.0	35.0	37.0	31.0	37.0
9	35.30091919616098	37.0	34.0	39.0	30.0	39.0
10	35.44611342615025	37.0	34.0	39.0	31.0	39.0
11	35.72806724740052	37.0	35.0	39.0	31.0	39.0
12	35.45790038512202	37.0	34.0	39.0	30.0	39.0
13	35.696536055388655	37.0	35.0	39.0	31.0	39.0
14	35.93643153765259	38.0	34.0	40.0	27.0	41.0
15	36.38904860864353	38.0	34.0	40.0	30.0	41.0
16	35.953957640167545	38.0	34.0	40.0	28.0	41.0
17	36.05731606206459	38.0	34.0	40.0	30.0	41.0
18	35.6288687175401	38.0	34.0	39.0	27.0	40.0
19	34.875160168405635	37.0	33.0	39.0	27.0	40.0
20	35.61707816936037	37.0	34.0	39.0	30.0	40.0
21	35.4530693111951	37.0	34.0	39.0	30.0	40.0
22	36.07531952923948	38.0	34.0	40.0	30.0	40.0
23	36.60180967865821	38.0	35.0	40.0	32.0	41.0
24	36.595331158273304	38.0	35.0	40.0	32.0	41.0
25	34.6065617900098	36.0	33.0	39.0	25.0	41.0
26	35.291623147520035	37.0	34.0	39.0	29.0	41.0
27	35.99389475724392	38.0	35.0	40.0	31.0	41.0
28	36.10977592574647	38.0	35.0	40.0	31.0	41.0
29	36.170218187952464	38.0	35.0	40.0	31.0	41.0
30	35.311464289175305	37.0	34.0	40.0	27.0	41.0
31	35.31865347273817	37.0	34.0	40.0	29.0	41.0
32	35.14249873480419	37.0	34.0	40.0	27.0	41.0
33	35.159339298596976	38.0	35.0	40.0	25.0	41.0
34	34.956857720206884	38.0	35.0	40.0	23.0	41.0
35	34.77515047754412	38.0	34.0	40.0	22.0	41.0
36	34.48703039700229	38.0	34.0	40.0	20.0	41.0
37	34.50597782587317	38.0	34.0	40.0	20.0	41.0
38	34.10296719822837	38.0	33.0	40.0	18.0	41.0
39	34.08850627931934	38.0	34.0	40.0	18.0	41.0
40	33.87226367757427	37.0	33.0	40.0	18.0	41.0
41	33.68086916260189	37.0	33.0	40.0	18.0	41.0
42	33.924020056494136	38.0	33.0	40.0	18.0	41.0
43	33.97021675226927	38.0	34.0	40.0	18.0	41.0
44	33.985478064555494	38.0	34.0	40.0	18.0	41.0
45	33.980036825273764	37.0	34.0	40.0	18.0	41.0
46	33.82225524293554	37.0	33.0	40.0	18.0	41.0
47	33.853398800486694	37.0	33.0	40.0	18.0	41.0
48	33.80176804384576	37.0	33.0	40.0	18.0	41.0
49	33.813231974100276	37.0	33.0	40.0	18.0	41.0
50	33.750876664046544	37.0	33.0	40.0	18.0	41.0
51	32.67351128626446	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	27.0
10	22.0
11	29.0
12	21.0
13	27.0
14	43.0
15	44.0
16	99.0
17	131.0
18	265.0
19	479.0
20	747.0
21	1114.0
22	1586.0
23	2378.0
24	3586.0
25	5410.0
26	7276.0
27	7656.0
28	7277.0
29	7320.0
30	8263.0
31	10728.0
32	14319.0
33	19231.0
34	24109.0
35	32611.0
36	41238.0
37	47397.0
38	30629.0
39	4540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.48604695401866	18.195489801265555	20.743468538797543	23.574994705918247
2	41.50093498867605	19.191495012795524	22.519408642094948	16.788161356433477
3	29.75991787892166	18.108272047607255	36.18675366906785	15.94505640440324
4	26.70550189689641	21.668048511734916	34.72307465911497	16.9033749322537
5	22.495719869496398	26.016015045959804	33.33189765014554	18.156367434398252
6	22.691331703832915	29.66085573896408	33.290980679293504	14.356831877909501
7	68.35861930347829	5.337870092206753	21.817000642468226	4.4865099618467195
8	68.6443202578487	5.669153987789515	20.127919372032174	5.558606382329611
9	62.694849127643	6.956243965644101	22.538431444333177	7.810475462379716
10	34.48223880436304	23.18089967086963	28.354742958871267	13.982118565896066
11	27.47359240236457	21.972413347546595	33.4105013046771	17.143492945411737
12	24.284222200686976	20.36803738519021	37.816254087210574	17.53148632691224
13	21.779313958788716	22.17879280579155	38.34314981712985	17.698743418289887
14	17.863846984885846	24.99524429944044	37.352887338351046	19.788021377322664
15	17.954653946513623	22.230118479755074	41.724901565971436	18.09032600775987
16	19.45099474898874	22.006151902459685	38.80687548678633	19.735977861765246
17	19.136580130862523	22.024815783900966	36.23664365984358	22.601960425392928
18	20.90605965981487	21.082648691913157	38.158664527498715	19.85262712077326
19	21.296924407690952	22.342101768402767	36.215467332823664	20.145506491082614
20	23.258067642213394	21.36691396309576	36.87444591601971	18.50057247867113
21	21.848585672599626	23.09009270924185	36.187112589864796	18.874209028293727
22	20.554317278806085	22.325950332540117	35.39892251976756	21.72080986888623
23	19.639428167386303	23.849569115583265	36.11102138091187	20.399981336118557
24	20.09848786668246	22.486746849572707	37.120665582725856	20.294099701018975
25	19.88385323010771	22.199251291217568	35.922587962514314	21.994307516160408
26	18.465039319773304	23.613040310394705	36.84968038103032	21.072239988801673
27	20.79192284638549	23.043791926435595	36.43189657338315	19.73238865379577
28	18.26440259427952	23.822650055812183	37.23587915854608	20.677068191362213
29	20.13043181761081	22.69061386223902	36.778255142437715	20.400699177712454
30	21.426494815389088	21.66122901659291	36.638993873222	20.273282294796008
31	22.848539012896023	22.819825349140206	33.30246614479583	21.02916949316794
32	22.053888368453734	23.735073381356937	34.742097461353204	19.46894078883613
33	21.70752979939917	23.088298105257113	33.507050999056034	21.697121096287685
34	20.826020322095523	23.529052843908936	34.36523062455808	21.279696209437464
35	22.185253380136604	22.62672596038232	34.183975622099474	21.0040450373816
36	22.453008294659618	25.187266925807485	32.733576681633664	19.626148097899236
37	22.9077609443924	24.978375021983897	32.21350044685639	19.90036358676731
38	20.781514143274006	25.843015221831	32.14925362420274	21.22621701069225
39	23.195256502747537	23.47413796197593	32.75654761263832	20.57405792263821
40	23.52295119036082	22.30513292631715	34.13336778972984	20.038548093592187
41	19.996554360349304	24.465477203145582	33.05409295330799	22.48387548319712
42	21.421828845028767	24.320473201178697	32.67937964129455	21.57831831249798
43	22.215402727080217	23.011130133913348	32.90585866416858	21.86760847483786
44	21.58980377800031	24.51608503551521	31.381880960328484	22.512230226155992
45	21.74952353264205	23.107320907495343	30.9910162124524	24.152139347410206
46	21.817000642468226	23.917405146206384	32.73214099844587	21.533453212879515
47	21.09915904857275	23.310111157770816	35.04897474274352	20.541755050912915
48	20.53601231816175	23.02512804499431	34.139110522481005	22.299749114362932
49	20.97066540326546	22.511871305359048	35.2262816164357	21.29118167493979
50	20.13653347115892	22.42537139329464	35.0443087723832	22.39378636316324
51	20.106025203418362	22.215761647877162	32.46223255914117	25.2159805895633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	248.0
2	406.0
3	5866.5
4	11327.0
5	7752.5
6	4178.0
7	3992.0
8	3806.0
9	3624.5
10	3443.0
11	3412.0
12	3381.0
13	3122.5
14	2864.0
15	2609.0
16	2354.0
17	2198.5
18	2043.0
19	1938.0
20	1833.0
21	1896.0
22	1959.0
23	1816.0
24	1673.0
25	1752.5
26	1956.5
27	2081.0
28	2732.5
29	3384.0
30	3650.0
31	3916.0
32	4410.5
33	4905.0
34	5547.5
35	6190.0
36	6700.5
37	7211.0
38	7454.5
39	7698.0
40	8319.5
41	8941.0
42	10563.5
43	12186.0
44	13784.5
45	15383.0
46	18898.0
47	22413.0
48	22941.0
49	23469.0
50	23095.0
51	22721.0
52	20127.5
53	17534.0
54	16431.5
55	15329.0
56	14069.0
57	12809.0
58	12063.5
59	11318.0
60	10787.0
61	10256.0
62	9351.5
63	8447.0
64	7831.0
65	7215.0
66	6041.5
67	4868.0
68	4117.5
69	3367.0
70	2756.0
71	2145.0
72	1768.0
73	1391.0
74	1212.0
75	847.5
76	662.0
77	448.0
78	234.0
79	205.0
80	176.0
81	135.5
82	95.0
83	63.5
84	32.0
85	19.0
86	6.0
87	4.0
88	2.0
89	2.5
90	3.0
91	3.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	278613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.64706372575237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.17016959061242	28.29936164853399
2	8.618793692859434	6.489445507883833
3	3.11128743256812	3.513925087290734
4	1.8496222616762643	2.785313886155862
5	1.3309327430129754	2.505285489544994
6	1.0942615402738423	2.4717440363597567
7	0.9305173096511551	2.4521871118046876
8	0.8286790633639518	2.495786680532758
9	0.7408190534911663	2.510069590443014
>10	6.218087612049737	38.23138891092721
>50	0.06889018065815192	1.8327492017300002
>100	0.030950660875401582	1.8019277426360996
>500	0.00599045049201321	1.9207031215833268
>1k	0.0	0.0
>5k	9.98408415335535E-4	2.6901119845737513
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7157	2.5687961437549576	No Hit
GCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	991	0.3556905097752079	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG	956	0.3431282818820371	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC	860	0.3086718853750543	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC	859	0.30831296457810653	No Hit
CCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	848	0.30436483581168144	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGCT	596	0.21391679498085156	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC	342	0.12275091255612625	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCC	293	0.1051637935056871	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22396657729538821	0.0
2	0.0	0.0	0.0	1.1370610847304325	0.0
3	0.0	0.0	0.0	1.442861603729905	0.0
4	0.0	0.0	0.0	1.9191495012795527	0.0
5	0.0	0.0	0.0	3.1226109334453165	0.0
6	0.0	0.0	0.0	3.7410314665862683	0.0
7	0.0	0.0	0.0	4.338634593504252	0.0
8	0.0	0.0	0.0	5.2646502496294145	0.0
9	0.0	0.0	0.0	5.670230750180358	0.0
10	0.0	0.0	0.0	6.734789833927347	0.0
11	0.0	0.0	0.0	7.753765976461974	0.0
12	0.0	0.0	0.0	8.808993119488322	0.0
13	0.0	0.0	0.0	9.209907649678945	0.0
14	0.0	0.0	0.0	9.382907473807755	0.0
15	0.0	0.0	0.0	9.714550290187464	0.0
16	0.0	0.0	0.0	10.227448109025781	0.0
17	0.0	0.0	0.0	10.869557414765284	0.0
18	0.0	0.0	0.0	11.612164543650152	0.0
19	0.0	0.0	0.0	12.098502223514338	0.0
20	0.0	0.0	0.0	12.478958268278939	0.0
21	0.0	0.0	0.0	12.890999343174942	0.0
22	0.0	0.0	0.0	13.344675230516883	0.0
23	0.0	0.0	0.0	13.772867741275533	0.0
24	0.0	0.0	0.0	14.145427528507284	0.0
25	0.0	0.0	0.0	14.461995671415188	0.0
26	0.0	0.0	0.0	14.77425676475972	0.0
27	0.0	0.0	0.0	15.076109154992768	0.0
28	0.0	0.0	0.0	15.39124161471288	0.0
29	0.0	0.0	0.0	15.721089827107852	0.0
30	0.0	0.0	0.0	16.078933861664748	0.0
31	0.0	0.0	0.0	16.436777896221642	0.0
32	0.0	0.0	0.0	16.747244385581435	0.0
33	0.0	0.0	0.0	17.06488929088018	0.0
34	0.0	0.0	0.0	17.411965701528644	0.0
35	3.5892079694773755E-4	0.0	0.0	17.744685280299198	0.0
36	3.5892079694773755E-4	0.0	0.0	18.056228532049833	0.0
37	3.5892079694773755E-4	0.0	0.0	18.360234447064567	0.0
38	3.5892079694773755E-4	0.0	0.0	18.677520431566368	0.0
39	3.5892079694773755E-4	0.0	0.0	19.03895367409274	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	35	1.2074997E-7	45.000004	5
CGAACGG	20	7.0240756E-4	45.000004	2
CGTATGG	20	7.0240756E-4	45.000004	2
GCGCGCT	20	7.0240756E-4	45.000004	29
CACGACG	20	7.0240756E-4	45.000004	26
TGAAAGG	55	1.8189894E-12	45.000004	2
ACCTACT	20	7.0240756E-4	45.000004	25
CGGATAC	20	7.0240756E-4	45.000004	14
ATAAGTG	20	7.0240756E-4	45.000004	37
GTCCGCA	20	7.0240756E-4	45.000004	23
CGGTCTA	20	7.0240756E-4	45.000004	31
ACGTAGG	20	7.0240756E-4	45.000004	2
CGGTATG	30	2.1595624E-6	45.000004	2
TCGACGG	30	2.1595624E-6	45.000004	2
TCGACAC	20	7.0240756E-4	45.000004	34
TATCGGG	20	7.0240756E-4	45.000004	3
TGCAACA	20	7.0240756E-4	45.000004	20
GGCAACG	25	3.8831742E-5	45.0	1
ACGGGTC	45	3.8198777E-10	45.0	5
TGACCGG	25	3.8831742E-5	45.0	2
>>END_MODULE