FastQCFastQC Report
Sat 18 Jun 2016
SRR3552341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387544
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50501.303077844064158No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG9660.2492620192803914No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC9020.23274776541502384No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC8950.23094151889849926No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC8630.2226843919658155No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC6050.15611130607105259No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT4710.12153458704043928Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT351.2091368E-745.00000414
CCGCTCG207.0274924E-445.019
GGACGCA207.0274924E-445.09
GTAAGTG207.0274924E-445.01
CGCGTAA207.0274924E-445.031
ACGAGTT207.0274924E-445.036
GCGTAAG207.0274924E-445.01
ACGGTCT453.8380676E-1045.030
CGTTTTT25450.043.3202361
CGCGAGG1100.042.9545442
CACGACG501.0768417E-940.526
GCGATCA501.0768417E-940.59
CGTAAGG501.0768417E-940.52
AATAAGG451.9226718E-840.02
TTGCTAG403.4503137E-739.3751
CAATTGG403.4503137E-739.3752
ACGGGAT800.039.3755
TACGAAT403.4503137E-739.37512
AGACCTA403.4503137E-739.37532
AAGCTAC403.4503137E-739.3758