##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552341_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387544
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.954183266932272	30.0	25.0	31.0	16.0	33.0
2	28.60433653985096	31.0	28.0	33.0	16.0	34.0
3	28.231122143550152	31.0	26.0	33.0	16.0	34.0
4	32.46872355139029	35.0	32.0	35.0	26.0	37.0
5	33.4689841669591	35.0	33.0	35.0	28.0	37.0
6	33.28441157649196	35.0	33.0	36.0	28.0	37.0
7	34.25656957661582	35.0	35.0	37.0	31.0	37.0
8	34.53425933571414	35.0	35.0	37.0	32.0	37.0
9	35.805665937287124	37.0	35.0	39.0	30.0	39.0
10	35.58465619387734	37.0	35.0	39.0	30.0	39.0
11	35.6656792519043	37.0	35.0	39.0	30.0	39.0
12	35.47266632950065	37.0	35.0	39.0	30.0	39.0
13	35.70570825506265	37.0	35.0	39.0	31.0	39.0
14	36.64211547592016	38.0	35.0	40.0	31.0	41.0
15	36.960884441508576	38.0	35.0	40.0	32.0	41.0
16	36.6165287038375	38.0	35.0	40.0	31.0	41.0
17	35.63318745742419	38.0	33.0	40.0	29.0	41.0
18	35.47297339140846	37.0	33.0	39.0	28.0	41.0
19	34.96959313007039	37.0	33.0	39.0	27.0	40.0
20	35.601725223458494	37.0	34.0	39.0	30.0	40.0
21	35.835469004809774	37.0	34.0	39.0	30.0	40.0
22	36.239046405053365	38.0	35.0	40.0	31.0	41.0
23	36.50261905744896	38.0	35.0	40.0	31.0	41.0
24	36.46965763887455	38.0	35.0	40.0	31.0	41.0
25	35.76987129203394	38.0	34.0	40.0	30.0	41.0
26	35.97525958342795	38.0	35.0	40.0	30.0	41.0
27	36.253790537332534	38.0	35.0	40.0	31.0	41.0
28	36.24006822451128	38.0	35.0	40.0	31.0	41.0
29	36.30759604070764	38.0	35.0	40.0	31.0	41.0
30	35.69718277150466	38.0	34.0	40.0	29.0	41.0
31	35.81081890056355	38.0	35.0	40.0	30.0	41.0
32	35.69979408789712	38.0	35.0	40.0	29.0	41.0
33	35.57176475445369	38.0	35.0	40.0	29.0	41.0
34	35.34109675288483	38.0	34.0	40.0	27.0	41.0
35	35.137372788638196	38.0	34.0	40.0	25.0	41.0
36	34.9080078649134	38.0	34.0	40.0	24.0	41.0
37	34.93622401585368	38.0	34.0	40.0	25.0	41.0
38	34.78812212290733	38.0	34.0	40.0	24.0	41.0
39	34.66020632495923	38.0	34.0	40.0	24.0	41.0
40	34.60333536321037	38.0	34.0	40.0	23.0	41.0
41	34.469869227752206	37.0	34.0	40.0	23.0	41.0
42	34.40818332886072	37.0	34.0	40.0	23.0	41.0
43	34.2720903948971	37.0	33.0	40.0	23.0	41.0
44	34.15487789773548	37.0	33.0	40.0	23.0	41.0
45	34.10159620585017	37.0	33.0	40.0	23.0	41.0
46	33.88875327704725	36.0	33.0	40.0	23.0	41.0
47	33.8825552711434	36.0	33.0	40.0	22.0	41.0
48	33.78506440559008	36.0	33.0	40.0	22.0	41.0
49	33.7211619841876	36.0	33.0	40.0	22.0	40.0
50	33.57606104081085	35.0	33.0	39.0	22.0	40.0
51	31.05176444481143	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	31.0
10	29.0
11	29.0
12	40.0
13	41.0
14	67.0
15	94.0
16	129.0
17	225.0
18	382.0
19	670.0
20	984.0
21	1373.0
22	1936.0
23	2727.0
24	3987.0
25	5527.0
26	7378.0
27	8377.0
28	8911.0
29	9698.0
30	11409.0
31	14439.0
32	19131.0
33	27751.0
34	37137.0
35	37726.0
36	48878.0
37	63833.0
38	60737.0
39	13848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.022665813430216	20.016823896125345	23.553970645913754	17.406539644530685
2	36.31587639080982	21.836488243915532	25.031738331647503	16.81589703362715
3	31.91044113700638	21.095411101707164	30.78927812067791	16.20486964060855
4	28.7618954234874	23.27426047106909	29.681274900398407	18.282569205045103
5	23.8491629337572	27.858514130008466	28.96677538550461	19.325547550729723
6	24.40032615651384	31.859866234543688	29.059151993064013	14.680655615878454
7	74.9821955700514	5.7936647193608986	14.678333298928639	4.545806411659063
8	76.62484775922218	4.7269471337448135	14.018537249963876	4.6296678570691325
9	71.00148628284788	6.077503457671903	16.038178890654997	6.882831368825218
10	42.14385979398468	20.67274941684041	22.61369031645439	14.569700472720518
11	33.46097475383441	21.088186115641065	26.952552484363068	18.498286646161468
12	29.097599240344323	20.16132361744731	32.214406622215805	18.526670519992567
13	25.27558161137832	20.592242429246742	34.19534298040997	19.93683297896497
14	22.043690522882564	22.759480213859586	34.91655141093656	20.280277852321284
15	22.65342773981793	20.782414383915114	37.13178374584563	19.43237413042132
16	26.44009454410338	20.571341576698387	33.65785562413558	19.33070825506265
17	24.800538777532356	20.97748900769977	32.7436368515575	21.47833536321037
18	26.571692504593024	21.050254938794048	32.89071692504593	19.487335631566996
19	24.93033049150548	24.353879817517495	30.547499122680264	20.16829056829676
20	27.16414136201309	23.66234543690523	30.31810581508164	18.855407386000042
21	25.607672935202196	25.103730157091842	30.943325145015795	18.34527176269017
22	24.04655987449167	21.417697087298475	32.47528022624528	22.060462811964577
23	24.55875977953471	23.365604937761905	31.568028404516646	20.507606878186735
24	26.09045682554755	20.28776087360403	32.52869351609108	21.093088784757345
25	23.583644695828088	21.911834527176268	30.95055013108189	23.553970645913754
26	20.984197923332577	21.91389880890944	32.929422207542885	24.172481060215098
27	21.127923539004602	23.17620708874347	32.02191235059761	23.673957021654317
28	20.099137130235533	24.853177961728218	32.426253535082466	22.621431372953783
29	23.261874780670066	23.455917263588137	31.386629647214253	21.895578308527547
30	24.325495943686395	22.04807712156555	33.37943562537415	20.246991309373904
31	27.24697066655657	22.25631154139917	30.30959065293231	20.187127139111947
32	26.94532749829697	22.507895877629377	30.079939310117044	20.46683731395661
33	25.510393658526514	23.28148545713519	29.504262741778998	21.703858142559294
34	23.718597114134138	23.68634271205334	32.5844291228867	20.01063105092583
35	22.526990483661212	25.97898561195632	30.16792931899346	21.326094585389015
36	23.542617096381314	29.378083520838928	28.4953450446917	18.583954338088063
37	25.12979171397312	27.65208595669137	26.680325330801146	20.53779699853436
38	24.060493776190576	28.12712879053733	26.545372912495097	21.267004520776997
39	25.405889395784737	24.8508556447784	27.39430877526165	22.348946184175215
40	25.511167764176456	22.457579010383338	29.01812439361724	23.013128831822968
41	22.517443180645294	25.761977994756723	27.403340007844267	24.31723881675371
42	23.22807216728939	24.43025824164482	27.85386949610883	24.48780009495696
43	22.932105773796007	23.077379600767912	28.04042895774415	25.950085667691923
44	21.73998307288979	22.72309724831245	29.38505047168838	26.151869207109385
45	23.93276634395062	22.68981070536507	28.524761059389387	24.852661891294925
46	23.482752926119357	24.111584749086557	29.100437627727434	23.305224697066656
47	21.807846334867783	23.811747827343478	31.596154243131103	22.78425159465764
48	22.62865635901988	23.446111925355574	30.92061804595091	23.00461366967364
49	23.581580414094915	22.116456453976838	31.877928699708935	22.424034432219308
50	21.91828540759243	22.41242284747022	31.26380488409058	24.405486860846768
51	21.684763536527466	21.448919348512685	30.74515409863138	26.12116301632847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	151.0
1	467.0
2	783.0
3	5399.0
4	10015.0
5	6813.0
6	3611.0
7	3246.5
8	2882.0
9	2736.5
10	2591.0
11	2478.0
12	2365.0
13	2283.0
14	2201.0
15	2105.0
16	2009.0
17	1925.5
18	1842.0
19	1734.5
20	1627.0
21	1566.5
22	1506.0
23	1508.5
24	1511.0
25	1708.0
26	2189.5
27	2474.0
28	2889.0
29	3304.0
30	3725.5
31	4147.0
32	4768.0
33	5389.0
34	6332.5
35	7276.0
36	7494.5
37	7713.0
38	8469.5
39	9226.0
40	10590.5
41	11955.0
42	13548.5
43	15142.0
44	17275.5
45	19409.0
46	23286.0
47	27163.0
48	28887.0
49	30611.0
50	31274.0
51	31937.0
52	29939.0
53	27941.0
54	26687.0
55	25433.0
56	24359.0
57	23285.0
58	22642.5
59	22000.0
60	21074.0
61	20148.0
62	19049.0
63	17950.0
64	16162.5
65	14375.0
66	11611.5
67	8848.0
68	7327.0
69	5806.0
70	4962.0
71	4118.0
72	3502.0
73	2886.0
74	2333.5
75	1367.5
76	954.0
77	765.5
78	577.0
79	418.5
80	260.0
81	207.0
82	154.0
83	143.5
84	133.0
85	102.5
86	72.0
87	47.5
88	23.0
89	24.5
90	26.0
91	21.5
92	17.0
93	12.5
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	387544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.41608713221852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69045078471687	29.4400531607508
2	7.582966241746098	5.977817162106716
3	3.1161837614795176	3.6848331198744337
4	2.1506586910113574	3.3908220142626684
5	1.8000390254800813	3.5475247534858303
6	1.4215108881676362	3.361823821644774
7	1.2285867287083845	3.3898257083779906
8	1.124911760248098	3.5471695966397077
9	0.9804492884564824	3.478092712427013
>10	5.828397235435649	34.957167205814685
>50	0.051229041932789376	1.3691552980142954
>100	0.020624679219694422	1.4218655479474258
>500	0.0033265611644668425	1.109537649089922
>1k	0.0	0.0
>5k	6.653122328933685E-4	1.3243122495637218
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5050	1.303077844064158	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCG	966	0.2492620192803914	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGC	902	0.23274776541502384	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	895	0.23094151889849926	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTC	863	0.2226843919658155	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGC	605	0.15611130607105259	No Hit
CTGTCTCTTATACACATCTGACGCTAGCTGGATCGTATGCCGTCTTCTGCT	471	0.12153458704043928	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.580352166463679E-4	0.0	0.0	0.15017649608818612	0.0
2	2.580352166463679E-4	0.0	0.0	0.8169394959024008	0.0
3	2.580352166463679E-4	0.0	0.0	1.0651693743162067	0.0
4	2.580352166463679E-4	0.0	0.0	1.3923580290238011	0.0
5	2.580352166463679E-4	0.0	0.0	2.4781702206717173	0.0
6	2.580352166463679E-4	0.0	0.0	3.0063683091468323	0.0
7	2.580352166463679E-4	0.0	0.0	3.58410915921805	0.0
8	2.580352166463679E-4	0.0	0.0	4.394339739487645	0.0
9	2.580352166463679E-4	0.0	0.0	4.742171211526949	0.0
10	2.580352166463679E-4	0.0	0.0	5.663098899737836	0.0
11	2.580352166463679E-4	0.0	0.0	6.568286439733295	0.0
12	2.580352166463679E-4	0.0	0.0	7.516049790475404	0.0
13	2.580352166463679E-4	0.0	0.0	7.822079557417997	0.0
14	2.580352166463679E-4	0.0	0.0	7.941291827508619	0.0
15	2.580352166463679E-4	0.0	0.0	8.189263670705778	0.0
16	2.580352166463679E-4	0.0	0.0	8.675143983650889	0.0
17	2.580352166463679E-4	0.0	0.0	9.262948207171315	0.0
18	2.580352166463679E-4	0.0	0.0	9.910358565737052	0.0
19	2.580352166463679E-4	0.0	0.0	10.311087257188861	0.0
20	2.580352166463679E-4	0.0	0.0	10.675949053526825	0.0
21	2.580352166463679E-4	0.0	0.0	11.13963833784035	0.0
22	2.580352166463679E-4	0.0	0.0	11.571589290506369	0.0
23	2.580352166463679E-4	0.0	0.0	12.014635757488183	0.0
24	2.580352166463679E-4	0.0	0.0	12.386722539892245	0.0
25	2.580352166463679E-4	0.0	0.0	12.689913919451726	0.0
26	2.580352166463679E-4	0.0	0.0	13.017618644592615	0.0
27	2.580352166463679E-4	0.0	0.0	13.32055198893545	0.0
28	2.580352166463679E-4	0.0	0.0	13.636129058893959	0.0
29	2.580352166463679E-4	0.0	0.0	13.95273826971905	0.0
30	2.580352166463679E-4	0.0	0.0	14.305730446091282	0.0
31	2.580352166463679E-4	0.0	0.0	14.628016431682596	0.0
32	2.580352166463679E-4	0.0	0.0	14.961397931589703	0.0
33	2.580352166463679E-4	0.0	0.0	15.316196354478459	0.0
34	2.580352166463679E-4	0.0	0.0	15.676155481700143	0.0
35	2.580352166463679E-4	0.0	0.0	16.01882624940652	0.0
36	2.580352166463679E-4	0.0	0.0	16.33620956588155	0.0
37	2.580352166463679E-4	0.0	0.0	16.6915240592036	0.0
38	2.580352166463679E-4	0.0	0.0	17.109541110170717	0.0
39	2.580352166463679E-4	0.0	0.0	17.5618768449518	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	35	1.2091368E-7	45.000004	14
CCGCTCG	20	7.0274924E-4	45.0	19
GGACGCA	20	7.0274924E-4	45.0	9
GTAAGTG	20	7.0274924E-4	45.0	1
CGCGTAA	20	7.0274924E-4	45.0	31
ACGAGTT	20	7.0274924E-4	45.0	36
GCGTAAG	20	7.0274924E-4	45.0	1
ACGGTCT	45	3.8380676E-10	45.0	30
CGTTTTT	2545	0.0	43.320236	1
CGCGAGG	110	0.0	42.954544	2
CACGACG	50	1.0768417E-9	40.5	26
GCGATCA	50	1.0768417E-9	40.5	9
CGTAAGG	50	1.0768417E-9	40.5	2
AATAAGG	45	1.9226718E-8	40.0	2
TTGCTAG	40	3.4503137E-7	39.375	1
CAATTGG	40	3.4503137E-7	39.375	2
ACGGGAT	80	0.0	39.375	5
TACGAAT	40	3.4503137E-7	39.375	12
AGACCTA	40	3.4503137E-7	39.375	32
AAGCTAC	40	3.4503137E-7	39.375	8
>>END_MODULE