FastQCFastQC Report
Sat 18 Jun 2016
SRR3552339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277939
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64792.3310870370836767No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC5590.20112326805522074No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG5480.19716556510601246No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC5060.18205433566358087No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC4990.17953579742317557No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA3920.14103814146269505No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.11333422081823709No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT2800.10074152961621075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT207.024045E-445.00000445
CATATGG207.024045E-445.0000042
CGTATGA207.024045E-445.00000415
TCCGAAT207.024045E-445.00000432
AAGGTCG207.024045E-445.00000426
GTATGAC207.024045E-445.00000416
GTATCAC207.024045E-445.00000424
CGTGTTT207.024045E-445.0000041
TTAGTCA207.024045E-445.00000432
TATAGCG207.024045E-445.0000041
GGACCGT207.024045E-445.0000048
TATCGAT207.024045E-445.00000413
GCGTATG207.024045E-445.0000041
AATCGTA207.024045E-445.00000412
CGTATGG302.1595424E-644.9999962
CGTTTTT28450.043.7346231
CTAGCGG556.002665E-1140.909092
TCTCGTA403.4456207E-739.37500428
GCTAATG403.4456207E-739.3750041
TACGGTT356.2312065E-638.57142633