##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277939
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.019597825422125	30.0	26.0	31.0	16.0	33.0
2	28.5988148478623	31.0	28.0	33.0	16.0	34.0
3	28.240538391517564	31.0	26.0	33.0	16.0	34.0
4	32.17616455409281	35.0	32.0	35.0	25.0	37.0
5	33.39047057088066	35.0	32.0	35.0	28.0	37.0
6	33.21622730167411	35.0	33.0	35.0	28.0	37.0
7	34.2104310658094	35.0	33.0	36.0	31.0	37.0
8	34.42919849319455	35.0	35.0	37.0	31.0	37.0
9	35.80276607457032	37.0	35.0	39.0	30.0	39.0
10	35.66727231514829	37.0	35.0	39.0	31.0	39.0
11	35.91311762652956	37.0	35.0	39.0	31.0	39.0
12	35.79662084126373	37.0	35.0	39.0	31.0	39.0
13	35.946139260773045	37.0	35.0	39.0	31.0	39.0
14	36.91629818053602	39.0	36.0	40.0	31.0	41.0
15	37.27477971785176	39.0	36.0	40.0	32.0	41.0
16	36.91873396680567	38.0	35.0	40.0	32.0	41.0
17	36.04393769855976	38.0	34.0	40.0	29.0	41.0
18	35.807673626227334	38.0	34.0	39.0	29.0	41.0
19	35.22087580368354	37.0	33.0	39.0	27.0	41.0
20	35.76409931675656	37.0	34.0	39.0	30.0	40.0
21	36.02393690701916	38.0	35.0	39.0	31.0	40.0
22	36.37485203587838	38.0	35.0	40.0	31.0	41.0
23	36.6386473290902	38.0	35.0	40.0	31.0	41.0
24	36.55070716955879	38.0	35.0	40.0	31.0	41.0
25	35.780286321818814	38.0	35.0	40.0	30.0	41.0
26	36.01614742803277	38.0	35.0	40.0	30.0	41.0
27	36.27718312291546	38.0	35.0	40.0	31.0	41.0
28	36.23320584732621	38.0	35.0	40.0	31.0	41.0
29	36.22530123516311	38.0	35.0	40.0	31.0	41.0
30	35.67384210204397	38.0	35.0	40.0	29.0	41.0
31	35.72245708590734	38.0	35.0	40.0	30.0	41.0
32	35.5101407143294	38.0	35.0	40.0	28.0	41.0
33	35.30547350317875	38.0	35.0	40.0	26.0	41.0
34	35.03147813009329	38.0	34.0	40.0	24.0	41.0
35	34.74948459913866	38.0	34.0	40.0	23.0	41.0
36	34.413022281867605	38.0	34.0	40.0	21.0	41.0
37	34.345025347288434	38.0	34.0	40.0	21.0	41.0
38	34.18669923976124	38.0	34.0	40.0	20.0	41.0
39	33.976815056541184	37.0	33.0	40.0	18.0	41.0
40	33.913427046941955	37.0	33.0	40.0	18.0	41.0
41	33.77301854003936	37.0	33.0	40.0	18.0	41.0
42	33.657575942922726	37.0	33.0	40.0	18.0	41.0
43	33.537193412943125	37.0	33.0	40.0	17.0	41.0
44	33.34585646490777	37.0	33.0	40.0	16.0	41.0
45	33.339016834629184	37.0	33.0	40.0	17.0	41.0
46	33.15510237857947	36.0	33.0	40.0	17.0	41.0
47	33.157721658349494	36.0	33.0	40.0	17.0	41.0
48	33.09059182050738	36.0	32.0	40.0	18.0	41.0
49	33.02383256757778	36.0	32.0	40.0	17.0	40.0
50	32.88576989915053	36.0	32.0	39.0	17.0	40.0
51	30.55622996412882	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	17.0
10	22.0
11	18.0
12	26.0
13	19.0
14	38.0
15	51.0
16	96.0
17	155.0
18	306.0
19	526.0
20	848.0
21	1178.0
22	1634.0
23	2444.0
24	3532.0
25	5032.0
26	6781.0
27	7425.0
28	7207.0
29	7265.0
30	8050.0
31	9729.0
32	12911.0
33	17524.0
34	23807.0
35	27400.0
36	34583.0
37	44951.0
38	43630.0
39	10725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.90701916607601	19.41649066881582	22.235454542183717	21.441035622924453
2	39.5795480303232	21.867388167907347	23.04966197618902	15.503401825580433
3	30.790209362486014	20.084982676054818	33.449066161999575	15.675741799459594
4	27.84423920356625	22.876602419955457	31.996229388462936	17.282928988015357
5	22.520768945703914	27.659666329662265	31.611612620035334	18.20795210459849
6	23.17990638233569	32.06351033859948	31.230953554556933	13.525629724507896
7	71.21382749452218	6.387012977667762	18.116205354412298	4.28295417339776
8	72.91959746563094	5.242877034169368	17.579396918028774	4.258128582170908
9	67.27375431299674	6.989663199479022	19.502840551344	6.233741936180241
10	37.64782919993236	22.425064492568513	25.819334458280412	14.107771849218715
11	28.426381328277067	22.49018669564185	30.731923191779494	18.35150878430159
12	25.4304001957264	20.99093686024631	36.44037000924663	17.13829293478065
13	21.685333832243767	23.18890116176571	37.39705474942344	17.728710256567087
14	19.38806716581696	25.0011693213259	36.1370660468664	19.47369746599074
15	18.51665293463674	22.60028279586528	40.5851643705993	18.297899898898677
16	20.32532318242492	21.15644080175866	38.199748865758316	20.318487150058107
17	20.933010480716995	21.112186486962965	34.96018910624274	22.994613926077303
18	21.564084205527113	21.839324456085688	36.526360100597614	20.07023123778959
19	22.752114672643998	22.741680728505177	34.196712228222744	20.30949237062809
20	22.655690637154198	22.574377831106826	34.362216169735085	20.40771536200389
21	22.94028545831999	23.456585797603072	34.57305379957473	19.03007494450221
22	21.23307632250242	22.0850618301138	33.84699520398361	22.83486664340017
23	20.66496605370243	23.894091869079187	34.06610803089887	21.374834046319517
24	20.445133644432772	22.829469775742158	35.52110355149871	21.20429302832636
25	20.146866758533346	23.45766517113467	34.43453419635244	21.960933873979542
26	19.421887536473832	24.333756687618507	34.135547728098615	22.10880804780905
27	20.092538290776034	24.399598473046243	34.98357553276079	20.524287703416938
28	19.760091243042538	24.155300263726932	35.618966751697315	20.465641741533215
29	19.167155383015697	24.518689352699692	35.10842307124945	21.20573219303516
30	21.11866272815258	25.25877980420164	33.77179884794865	19.850758619697128
31	23.150763296982433	24.22006267562307	31.657306099539827	20.971867927854674
32	22.177887953831597	25.17171033931906	32.56757777785773	20.082823928991616
33	21.564803787881516	25.428241448663197	31.72746537909397	21.279489384361316
34	20.74016240973739	24.60431965287347	31.602258049428112	23.053259887961026
35	20.406995779649492	25.18214428345788	30.867564465584174	23.543295471308454
36	23.90560518674961	26.602959642223656	29.84827606057444	19.643159110452295
37	21.52954425251584	27.00232784891649	30.81359578900406	20.654532109563608
38	21.2712141872857	27.267493946513444	29.57699351296508	21.88429835323578
39	22.07786600656979	25.39657982506953	30.3534228733643	22.172131294996383
40	21.84112341197169	24.992174541895885	31.153958242635976	22.012743803496452
41	20.541197888745373	25.634042002022028	29.65578778077204	24.168972328460562
42	20.83658644522719	25.593745390175542	30.451645864740108	23.118022299857163
43	22.238692662778522	24.15314151666373	31.184180701520837	22.42398511903691
44	21.083762983964107	25.03247115374237	31.102508104296266	22.78125775799726
45	20.729728465598566	25.455945369307653	30.144384199410663	23.669941965683115
46	21.824573017820455	25.6218091019972	30.860368642040157	21.693249238142183
47	21.20501261068076	24.519049143876895	31.7886298792181	22.487308366224244
48	20.52932477989775	24.982819971288663	31.758767211510442	22.72908803730315
49	20.56926160056703	24.105649081273228	33.27420764988001	22.050881668279732
50	19.56148651322772	23.802345118892994	33.80382026271952	22.832348105159767
51	20.34475190599376	23.654111153886284	31.412288307866117	24.58884863225384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	137.0
1	376.0
2	615.0
3	5569.0
4	10523.0
5	7321.0
6	4119.0
7	3708.5
8	3298.0
9	3086.0
10	2874.0
11	2800.0
12	2726.0
13	2581.0
14	2436.0
15	2205.0
16	1974.0
17	1827.0
18	1680.0
19	1552.5
20	1425.0
21	1737.5
22	2050.0
23	1820.0
24	1590.0
25	1622.0
26	1963.5
27	2273.0
28	2802.5
29	3332.0
30	3694.0
31	4056.0
32	4464.0
33	4872.0
34	5597.5
35	6323.0
36	6445.5
37	6568.0
38	7676.0
39	8784.0
40	9889.5
41	10995.0
42	11743.0
43	12491.0
44	14361.5
45	16232.0
46	18952.5
47	21673.0
48	21743.5
49	21814.0
50	21231.0
51	20648.0
52	18802.5
53	16957.0
54	15329.5
55	13702.0
56	12906.5
57	12111.0
58	11919.5
59	11728.0
60	11615.5
61	11503.0
62	10685.0
63	9867.0
64	8507.0
65	7147.0
66	6487.5
67	5828.0
68	4914.5
69	4001.0
70	3564.0
71	3127.0
72	2463.0
73	1799.0
74	1554.0
75	1020.5
76	732.0
77	552.5
78	373.0
79	321.5
80	270.0
81	210.5
82	151.0
83	112.0
84	73.0
85	64.0
86	55.0
87	37.5
88	20.0
89	16.5
90	13.0
91	10.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	277939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.76866473976447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.39905849769777	28.45860033075267
2	7.272277095243767	5.3478383681937265
3	2.551914464560151	2.814914621759032
4	1.5145230165472514	2.227479561443306
5	1.1406192504592712	2.096952340792919
6	0.9336871450733095	2.0598257765417007
7	0.8287222458580986	2.1329707294238767
8	0.7197587619128986	2.117165488822666
9	0.6997654627041913	2.3156497525172908
>10	6.840707230220695	43.74850483910254
>50	0.06397855658834557	1.6705758928417291
>100	0.03098961334747989	2.035198837990023
>500	0.0029989948400786994	0.5928798493187479
>1k	0.0	0.0
>5k	9.996649466928996E-4	2.3814436104997943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6479	2.3310870370836767	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	559	0.20112326805522074	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	548	0.19716556510601246	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	506	0.18205433566358087	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	499	0.17953579742317557	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	392	0.14103814146269505	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	315	0.11333422081823709	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	280	0.10074152961621075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.597911772007527E-4	0.0	0.0	0.13672064733628603	0.0
2	3.597911772007527E-4	0.0	0.0	0.6656136778213925	0.0
3	3.597911772007527E-4	0.0	0.0	0.8760915164838328	0.0
4	3.597911772007527E-4	0.0	0.0	1.1984644112557072	0.0
5	3.597911772007527E-4	0.0	0.0	2.167741842634535	0.0
6	3.597911772007527E-4	0.0	0.0	2.6635340848171722	0.0
7	3.597911772007527E-4	0.0	0.0	3.142416141671374	0.0
8	3.597911772007527E-4	0.0	0.0	3.8803478461101175	0.0
9	3.597911772007527E-4	0.0	0.0	4.189048676148364	0.0
10	3.597911772007527E-4	0.0	0.0	4.991023210128842	0.0
11	3.597911772007527E-4	0.0	0.0	5.766013405819263	0.0
12	3.597911772007527E-4	0.0	0.0	6.571585851571748	0.0
13	3.597911772007527E-4	0.0	0.0	6.884244384559202	0.0
14	3.597911772007527E-4	0.0	0.0	7.035356678983518	0.0
15	3.597911772007527E-4	0.0	0.0	7.268861152986807	0.0
16	3.597911772007527E-4	0.0	0.0	7.699171400918907	0.0
17	3.597911772007527E-4	0.0	0.0	8.24065712260604	0.0
18	3.597911772007527E-4	0.0	0.0	8.896556438643012	0.0
19	3.597911772007527E-4	0.0	0.0	9.19194499512483	0.0
20	3.597911772007527E-4	0.0	0.0	9.506402483998288	0.0
21	3.597911772007527E-4	0.0	0.0	9.860077211186628	0.0
22	3.597911772007527E-4	0.0	0.0	10.21087360895736	0.0
23	3.597911772007527E-4	0.0	0.0	10.565627709677303	0.0
24	3.597911772007527E-4	0.0	0.0	10.851661695551902	0.0
25	3.597911772007527E-4	0.0	0.0	11.12294424316127	0.0
26	3.597911772007527E-4	0.0	0.0	11.362565167176971	0.0
27	3.597911772007527E-4	0.0	0.0	11.617657111812305	0.0
28	3.597911772007527E-4	0.0	0.0	11.891098406484875	0.0
29	3.597911772007527E-4	0.0	0.0	12.14655014229741	0.0
30	3.597911772007527E-4	0.0	0.0	12.454171598804054	0.0
31	3.597911772007527E-4	0.0	0.0	12.732649969957437	0.0
32	3.597911772007527E-4	0.0	0.0	13.007890220516012	0.0
33	3.597911772007527E-4	0.0	0.0	13.280971724011383	0.0
34	3.597911772007527E-4	0.0	0.0	13.554772809861156	0.0
35	3.597911772007527E-4	0.0	0.0	13.87642612227863	0.0
36	3.597911772007527E-4	0.0	0.0	14.15346532872321	0.0
37	3.597911772007527E-4	0.0	0.0	14.420430382206167	0.0
38	3.597911772007527E-4	0.0	0.0	14.701787082777155	0.0
39	3.597911772007527E-4	0.0	0.0	15.00545083633459	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	20	7.024045E-4	45.000004	45
CATATGG	20	7.024045E-4	45.000004	2
CGTATGA	20	7.024045E-4	45.000004	15
TCCGAAT	20	7.024045E-4	45.000004	32
AAGGTCG	20	7.024045E-4	45.000004	26
GTATGAC	20	7.024045E-4	45.000004	16
GTATCAC	20	7.024045E-4	45.000004	24
CGTGTTT	20	7.024045E-4	45.000004	1
TTAGTCA	20	7.024045E-4	45.000004	32
TATAGCG	20	7.024045E-4	45.000004	1
GGACCGT	20	7.024045E-4	45.000004	8
TATCGAT	20	7.024045E-4	45.000004	13
GCGTATG	20	7.024045E-4	45.000004	1
AATCGTA	20	7.024045E-4	45.000004	12
CGTATGG	30	2.1595424E-6	44.999996	2
CGTTTTT	2845	0.0	43.734623	1
CTAGCGG	55	6.002665E-11	40.90909	2
TCTCGTA	40	3.4456207E-7	39.375004	28
GCTAATG	40	3.4456207E-7	39.375004	1
TACGGTT	35	6.2312065E-6	38.571426	33
>>END_MODULE