##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	203037
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.658096800090625	28.0	16.0	31.0	16.0	33.0
2	26.852755901633692	30.0	25.0	33.0	16.0	33.0
3	27.12985317946975	31.0	25.0	33.0	16.0	34.0
4	30.498086555652417	33.0	28.0	35.0	19.0	37.0
5	32.3274378561543	33.0	32.0	35.0	28.0	37.0
6	31.03990898210671	35.0	28.0	35.0	17.0	37.0
7	33.13439422371292	35.0	32.0	35.0	28.0	37.0
8	33.886449267867434	35.0	35.0	37.0	31.0	37.0
9	35.33947999625684	37.0	34.0	39.0	30.0	39.0
10	35.36079630806208	37.0	34.0	39.0	30.0	39.0
11	35.67004043597965	37.0	35.0	39.0	31.0	39.0
12	35.414151115313956	37.0	34.0	39.0	30.0	39.0
13	35.64078960977556	37.0	35.0	39.0	30.0	39.0
14	35.93082049084649	38.0	34.0	40.0	27.0	41.0
15	36.357826406024515	38.0	34.0	40.0	30.0	41.0
16	35.8758748405463	38.0	34.0	40.0	27.0	41.0
17	36.07201643050281	38.0	34.0	40.0	30.0	41.0
18	35.62149263434743	38.0	34.0	39.0	27.0	40.0
19	34.94290203263444	37.0	33.0	39.0	27.0	41.0
20	35.67298078675315	37.0	34.0	39.0	30.0	40.0
21	35.523608997374865	37.0	34.0	39.0	30.0	40.0
22	36.13389677743466	38.0	34.0	40.0	31.0	41.0
23	36.672463639632184	38.0	35.0	40.0	32.0	41.0
24	36.66262799391244	38.0	35.0	40.0	32.0	41.0
25	34.625048636455425	36.0	33.0	40.0	25.0	41.0
26	35.42519836285997	37.0	34.0	40.0	29.0	41.0
27	36.093973019695916	38.0	35.0	40.0	31.0	41.0
28	36.247979432320214	38.0	35.0	40.0	31.0	41.0
29	36.27399439511025	38.0	35.0	40.0	31.0	41.0
30	35.35282731718849	37.0	34.0	40.0	28.0	41.0
31	35.37668011249181	37.0	34.0	40.0	29.0	41.0
32	35.27501391372016	38.0	34.0	40.0	28.0	41.0
33	35.32956554716628	38.0	35.0	40.0	27.0	41.0
34	35.17248087786955	38.0	35.0	40.0	25.0	41.0
35	35.00725483532558	38.0	35.0	40.0	23.0	41.0
36	34.742667592606274	38.0	34.0	40.0	22.0	41.0
37	34.75498061929599	38.0	34.0	40.0	22.0	41.0
38	34.35126110019356	38.0	34.0	40.0	21.0	41.0
39	34.339307613883186	38.0	34.0	40.0	21.0	41.0
40	34.11931815383403	37.0	33.0	40.0	20.0	41.0
41	33.95674679984436	37.0	33.0	40.0	20.0	41.0
42	34.18122805202992	38.0	34.0	40.0	20.0	41.0
43	34.22648088771997	38.0	34.0	40.0	20.0	41.0
44	34.24098070794978	38.0	34.0	40.0	20.0	41.0
45	34.243226604017984	37.0	34.0	40.0	20.0	41.0
46	34.089151238444224	37.0	34.0	40.0	20.0	41.0
47	34.08758994665997	37.0	34.0	40.0	20.0	41.0
48	34.049532843767395	37.0	34.0	40.0	20.0	41.0
49	34.08398469244522	37.0	34.0	40.0	20.0	41.0
50	34.03399380408497	37.0	34.0	40.0	20.0	41.0
51	32.97084275279875	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	23.0
10	22.0
11	21.0
12	24.0
13	28.0
14	35.0
15	50.0
16	61.0
17	130.0
18	207.0
19	345.0
20	499.0
21	705.0
22	1129.0
23	1624.0
24	2296.0
25	3596.0
26	4746.0
27	5201.0
28	5069.0
29	5375.0
30	6000.0
31	7679.0
32	10614.0
33	14312.0
34	17378.0
35	24018.0
36	30909.0
37	34953.0
38	22675.0
39	3298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.84894871378123	18.48727079300817	21.80292262001507	22.860857873195524
2	40.986125681526026	19.64765042824707	22.62346271861779	16.742761171609118
3	30.966277082502202	18.28533715529682	33.92435861443973	16.824027147761246
4	27.764397622108284	22.87858863162872	31.627732876273782	17.729280869989214
5	22.376709663755868	27.25365327501884	32.17295369809444	18.19668336313086
6	23.65381679201328	30.26640464545871	31.469141092510235	14.610637470017778
7	70.39554366938046	6.354999335096559	18.96353866536641	4.285918330156573
8	71.86177888759192	5.082324896447445	18.201116052739156	4.8547801632214815
9	66.34406536739608	6.902190241187567	20.17120032309382	6.582544068322522
10	37.26709910016401	22.007318863064366	26.64046454587095	14.085117490900673
11	28.30518575432064	22.128971566758768	31.033259947694265	18.532582731226327
12	25.016130064963527	20.69622778114334	36.9026335101484	17.385008643744737
13	21.827056152326914	22.788949797327582	37.464600048267066	17.91939400207844
14	19.391539473100963	24.05571398316563	36.86470938794407	19.68803715578934
15	18.467569950304625	22.18807409486941	40.77335658032772	18.570999374498246
16	20.198781502878784	20.81443283736462	38.66093372143993	20.32585193831666
17	20.808522584553554	20.719376271320005	35.46890468239779	23.00319646172865
18	21.25523919285647	21.477366194338963	37.199131192836774	20.06826341996779
19	22.73280239562247	22.28362318198161	35.0079049631348	19.97566945926112
20	22.36144151066062	22.258997128602175	35.10296152917941	20.276599831557796
21	22.670252220038712	22.809635682166306	35.18274994212878	19.337362155666206
22	21.128661278486188	21.748745302580318	34.64639449952472	22.476198919408777
23	20.379044213616236	23.665144776567818	34.775435019232944	21.180375990583
24	20.10520250003694	22.979062929416806	36.11459980200653	20.801134768539725
25	20.27906243689574	22.329920162334943	35.18472002639913	22.206297374370187
26	19.194038524997907	24.216275851199534	34.821731999586284	21.767953624216275
27	19.907209030866298	23.958194811783073	35.87129439461773	20.2633017627329
28	19.63188975408423	23.72769495215158	36.18158266719859	20.458832626565602
29	19.190590877524784	24.257155099809395	35.56199116417205	20.99026285849377
30	20.76567325167334	24.94422198909558	34.27454109349527	20.015563665735804
31	23.035210331121913	23.82521412353413	32.06312150002216	21.07645404532179
32	21.896501622856917	25.228899166161835	32.473884070391115	20.40071514059014
33	21.44830745135123	25.416549692913115	31.76810138053655	21.367041475199102
34	21.026709417495333	24.44628318976344	31.398218058777466	23.12878933396376
35	20.379044213616236	25.313120268719498	30.8795933746066	23.42824214305767
36	23.960657417121016	26.402576870225623	29.927550150957703	19.70921556169565
37	21.945753729615785	27.008377783359684	30.766313529061208	20.279554957963327
38	21.43057669291804	27.469377502622677	29.522205312332233	21.577840492127052
39	21.894039017518974	25.70171939104695	30.367863985381977	22.0363776060521
40	21.826071110191737	24.95308736831218	31.35290612055931	21.867935400936776
41	20.619394494599508	25.390446076330914	30.04821781251693	23.941941616552647
42	20.69622778114334	25.7430911607244	30.398400291572468	23.16228076655979
43	22.480631609017077	24.360584524003016	31.125361387333346	22.033422479646568
44	21.520708048286767	25.051099060762322	30.99829095189547	22.429901939055444
45	21.070051271443134	25.259927993419918	30.21124228588878	23.458778449248165
46	21.78814698798741	25.460876588996094	31.180031225835688	21.57094519718081
47	21.102065140836398	24.73785566177593	32.03652536237237	22.12355383501529
48	20.177603096972476	25.316075395125026	31.78041440722627	22.72590710067623
49	20.47508582179603	24.1788442500628	33.2087255032334	22.137344424907777
50	19.848106502755655	23.67105502937888	33.52541654969291	22.95542191817255
51	20.450952289484185	23.8355570659535	31.416441338278243	24.297049306284077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	231.5
2	378.0
3	4055.5
4	7733.0
5	5253.0
6	2773.0
7	2661.0
8	2549.0
9	2471.0
10	2393.0
11	2293.0
12	2193.0
13	2135.0
14	2077.0
15	1825.0
16	1573.0
17	1449.0
18	1325.0
19	1261.5
20	1198.0
21	1332.0
22	1466.0
23	1347.5
24	1229.0
25	1219.5
26	1398.0
27	1586.0
28	2006.0
29	2426.0
30	2713.0
31	3000.0
32	3188.0
33	3376.0
34	3892.5
35	4409.0
36	4548.5
37	4688.0
38	5506.5
39	6325.0
40	6965.0
41	7605.0
42	8251.0
43	8897.0
44	10017.0
45	11137.0
46	13338.5
47	15540.0
48	15632.0
49	15724.0
50	15626.5
51	15529.0
52	13933.5
53	12338.0
54	11161.5
55	9985.0
56	9396.0
57	8807.0
58	8671.5
59	8536.0
60	8479.5
61	8423.0
62	7848.0
63	7273.0
64	6448.5
65	5624.0
66	5083.0
67	4542.0
68	3813.5
69	3085.0
70	2715.5
71	2346.0
72	1865.0
73	1384.0
74	1223.5
75	800.0
76	537.0
77	431.5
78	326.0
79	264.5
80	203.0
81	142.0
82	81.0
83	52.0
84	23.0
85	24.0
86	25.0
87	16.5
88	8.0
89	4.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	203037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.96396223348454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.44640023084692	31.31547451942257
2	7.216370894050883	5.912222895334348
3	2.9949983167412113	3.680609938090102
4	1.8371567354398113	3.010288765101927
5	1.4764584235079112	3.02407935499441
6	1.26364641946809	3.105837852214129
7	1.1145577838695715	3.1959692075828543
8	1.0869042466214591	3.561912360801233
9	0.946231904968018	3.488526721730522
>10	5.5307074496224695	33.54610243453164
>50	0.06131871302842303	1.712495752005792
>100	0.02284422642235368	1.7681506326433112
>500	0.0012023277064396672	0.261036165821993
>1k	0.0012023277064396672	2.4172933997251733
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4908	2.4172933997251733	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	530	0.261036165821993	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	417	0.20538128518447374	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	397	0.19553086383270044	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	317	0.15612917842560717	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	255	0.12559287223510984	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	242	0.11919009835645719	No Hit
CTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGCT	224	0.11032471913986122	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11426488768057054	0.0
2	0.0	0.0	0.0	0.6516053724198052	0.0
3	0.0	0.0	0.0	0.8372858149007324	0.0
4	0.0	0.0	0.0	1.149051650684358	0.0
5	0.0	0.0	0.0	2.011948561099701	0.0
6	0.0	0.0	0.0	2.47393332249787	0.0
7	0.0	0.0	0.0	2.9344405206932724	0.0
8	0.0	0.0	0.0	3.6175672414387527	0.0
9	0.0	0.0	0.0	3.929825598289967	0.0
10	0.0	0.0	0.0	4.715396701093889	0.0
11	0.0	0.0	0.0	5.575831006171289	0.0
12	0.0	0.0	0.0	6.409669173598901	0.0
13	0.0	0.0	0.0	6.736703162477775	0.0
14	0.0	0.0	0.0	6.8682062875239485	0.0
15	0.0	0.0	0.0	7.115944384521048	0.0
16	0.0	0.0	0.0	7.531139644498293	0.0
17	0.0	0.0	0.0	8.072420297778237	0.0
18	0.0	0.0	0.0	8.733383570482227	0.0
19	0.0	0.0	0.0	9.099326723700607	0.0
20	0.0	0.0	0.0	9.42340558617395	0.0
21	0.0	0.0	0.0	9.78836369725715	0.0
22	0.0	0.0	0.0	10.132635923501628	0.0
23	0.0	0.0	0.0	10.480355797219225	0.0
24	0.0	0.0	0.0	10.810837433571221	0.0
25	0.0	0.0	0.0	11.09206696316435	0.0
26	0.0	0.0	0.0	11.31714909105237	0.0
27	0.0	0.0	0.0	11.572767525130887	0.0
28	0.0	0.0	0.0	11.835281254155646	0.0
29	0.0	0.0	0.0	12.082034309017569	0.0
30	0.0	0.0	0.0	12.355876022596867	0.0
31	0.0	0.0	0.0	12.650403621014888	0.0
32	0.0	0.0	0.0	12.937050882351492	0.0
33	0.0	0.0	0.0	13.197102006038309	0.0
34	0.0	0.0	0.0	13.461585819333422	0.0
35	0.0	0.0	0.0	13.775814260454991	0.0
36	0.0	0.0	0.0	14.074282027413723	0.0
37	0.0	0.0	0.0	14.349108783128198	0.0
38	0.0	0.0	0.0	14.63329343912686	0.0
39	0.0	0.0	0.0	14.947521880248427	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATTA	30	2.1566466E-6	45.000004	28
AGGCGTC	30	2.1566466E-6	45.000004	35
CGTCATA	30	2.1566466E-6	45.000004	38
TCGTTTA	20	7.019553E-4	45.0	38
ACCGGGT	20	7.019553E-4	45.0	5
GGCACCG	20	7.019553E-4	45.0	8
TTTCGCG	25	3.8794264E-5	45.0	1
CGCATCG	20	7.019553E-4	45.0	21
CAACCCG	20	7.019553E-4	45.0	23
TAACGCC	25	3.8794264E-5	45.0	12
GTTCGAC	25	3.8794264E-5	45.0	1
ACAATAC	20	7.019553E-4	45.0	14
CATCGTT	20	7.019553E-4	45.0	36
ATAACGC	25	3.8794264E-5	45.0	11
GCAATCG	20	7.019553E-4	45.0	45
ACTCATG	25	3.8794264E-5	45.0	22
TATCGCG	20	7.019553E-4	45.0	32
ATCGCGG	20	7.019553E-4	45.0	33
GCATCGT	20	7.019553E-4	45.0	35
CTAGCGG	20	7.019553E-4	45.0	2
>>END_MODULE