##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	484782
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.916783213898206	30.0	25.0	31.0	16.0	33.0
2	28.51553069214616	31.0	28.0	33.0	16.0	34.0
3	28.155300733113027	31.0	26.0	33.0	16.0	34.0
4	32.32621054412086	35.0	32.0	35.0	25.0	37.0
5	33.41614993956046	35.0	33.0	35.0	28.0	37.0
6	33.201377113836735	35.0	33.0	35.0	28.0	37.0
7	34.239901646513275	35.0	35.0	37.0	31.0	37.0
8	34.49652833644814	35.0	35.0	37.0	32.0	37.0
9	35.8231122442665	37.0	35.0	39.0	30.0	39.0
10	35.61006184223012	37.0	35.0	39.0	31.0	39.0
11	35.90085027909452	37.0	35.0	39.0	31.0	39.0
12	35.806484151639296	37.0	35.0	39.0	31.0	39.0
13	36.029741203262496	37.0	35.0	39.0	32.0	39.0
14	36.9788832918714	39.0	36.0	40.0	32.0	41.0
15	37.36031453313036	39.0	36.0	40.0	32.0	41.0
16	37.00613471622296	39.0	36.0	40.0	32.0	41.0
17	36.11383673486227	38.0	34.0	40.0	30.0	41.0
18	35.927121881588015	38.0	34.0	39.0	30.0	41.0
19	35.34953855547442	37.0	34.0	39.0	27.0	41.0
20	35.951906217640094	37.0	34.0	39.0	30.0	40.0
21	36.16307742449183	38.0	35.0	40.0	31.0	41.0
22	36.551076566374164	38.0	35.0	40.0	31.0	41.0
23	36.77913783927621	38.0	35.0	40.0	32.0	41.0
24	36.73276854338651	38.0	35.0	40.0	32.0	41.0
25	35.93425292193192	38.0	35.0	40.0	30.0	41.0
26	36.19505055880788	38.0	35.0	40.0	30.0	41.0
27	36.48703747251342	38.0	35.0	40.0	31.0	41.0
28	36.4764120780062	38.0	35.0	40.0	31.0	41.0
29	36.562343486350564	38.0	35.0	40.0	31.0	41.0
30	35.99739057968324	38.0	35.0	40.0	30.0	41.0
31	36.08052485447067	38.0	35.0	40.0	30.0	41.0
32	35.921187255302385	38.0	35.0	40.0	30.0	41.0
33	35.83014427103316	38.0	35.0	40.0	29.0	41.0
34	35.59238791869335	38.0	35.0	40.0	27.0	41.0
35	35.35364143058117	38.0	35.0	40.0	25.0	41.0
36	35.09242504878481	38.0	35.0	40.0	24.0	41.0
37	35.02657689435664	38.0	35.0	40.0	24.0	41.0
38	34.865382790615165	38.0	34.0	40.0	23.0	41.0
39	34.6983530741653	38.0	34.0	40.0	23.0	41.0
40	34.657167551600516	38.0	34.0	40.0	23.0	41.0
41	34.568389502910584	38.0	34.0	40.0	22.0	41.0
42	34.514631318819596	38.0	34.0	40.0	22.0	41.0
43	34.3920958286405	38.0	34.0	40.0	21.0	41.0
44	34.255768572265474	38.0	34.0	40.0	21.0	41.0
45	34.23093060385905	38.0	34.0	40.0	21.0	41.0
46	34.00081067366363	37.0	33.0	40.0	20.0	41.0
47	34.02877375810158	37.0	33.0	40.0	20.0	41.0
48	33.949525766220695	37.0	33.0	40.0	20.0	41.0
49	33.89575314264969	37.0	33.0	40.0	20.0	41.0
50	33.724674183447405	37.0	33.0	40.0	20.0	41.0
51	31.411750023722004	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	25.0
10	33.0
11	43.0
12	28.0
13	24.0
14	72.0
15	105.0
16	167.0
17	307.0
18	524.0
19	802.0
20	1214.0
21	1743.0
22	2514.0
23	3485.0
24	5363.0
25	7555.0
26	9974.0
27	10775.0
28	10842.0
29	10942.0
30	12569.0
31	15571.0
32	20720.0
33	29042.0
34	39133.0
35	47092.0
36	63743.0
37	85864.0
38	84036.0
39	20464.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.14559946532668	20.58801688181492	22.21307721821355	19.053306434644853
2	37.00962494482056	21.991740617432164	23.623814415551735	17.374820022195543
3	31.17483734957156	20.652169428732915	32.22933194714325	15.943661274552273
4	27.542895569554975	23.786155426562868	30.34745514478673	18.32349385909543
5	23.301607732960385	28.168743889005782	29.8123692711363	18.717279106897532
6	23.442289523950972	32.502856954259855	30.322289193905718	13.732564327883463
7	74.28431748703541	6.090778948063253	15.526154023870523	4.098749541030814
8	75.61893799687283	4.7885441291137045	15.145158029794835	4.447359844218639
9	69.7546526067387	6.898358437400728	17.20917855860985	6.137810397250723
10	35.618896741215636	24.732972758889563	24.7876365046557	14.860493995239096
11	27.456258689472797	25.09746649009245	30.203266622935672	17.243008197499083
12	24.525869359835966	22.66895223007455	34.22424925017843	18.58092915991105
13	22.05733711235153	24.33134893622288	35.18901279337929	18.4223011580463
14	19.814679587938496	25.714032286677313	34.7477835398179	19.723504585566296
15	19.47040112875478	24.37569876769352	37.21672834387416	18.937171759677547
16	21.427156948896616	23.8137967168748	35.761847593351234	18.997198740877344
17	21.19818805153657	23.123795850506003	33.72711858113544	21.950897516821993
18	22.781786452467294	23.021069264122843	34.52624066075061	19.670903622659257
19	22.688754945521904	24.66201302853654	32.44056091191504	20.20867111402651
20	23.371948628455677	24.73338531546138	32.98410419528778	18.910561860795163
21	23.105230804774106	24.870766653877414	32.975440507279565	19.048562034068922
22	21.69593755543729	23.187329562566266	32.596507296062974	22.52022558593347
23	20.701469939065394	25.337161858319824	33.195539438345484	20.7658287642693
24	21.49564133981872	23.348226625576032	34.54006130590657	20.616070728698674
25	20.703326443638584	24.387662908276297	33.112615567409684	21.79639508067544
26	19.640993271202316	25.905252257715837	33.471539784893004	20.982214686188843
27	20.94260925529413	25.173789455879138	33.49072366548263	20.392877623344102
28	18.68654364229695	25.392856995515512	34.18773799357237	21.732861368615172
29	19.914105721747095	24.764739614919694	33.56168339583566	21.759471267497553
30	21.339076120813065	24.56258689472794	33.17986228861633	20.91847469584267
31	21.413336303740653	25.501153095618236	31.158747643270583	21.92676295737053
32	20.541604267485177	26.382992767883295	31.757573507267185	21.31782945736434
33	20.098312231064686	25.931243321740492	30.242872053830382	23.72757239336444
34	19.10838273698281	25.731153384407833	31.59255087853922	23.567913000070135
35	19.891415110297	26.474992883399135	30.66780532280489	22.965786683498976
36	20.649281532730175	27.548258804988635	29.268413431191753	22.534046231089437
37	20.323155562706535	27.919147163054735	29.633732275538282	22.123964998700448
38	20.055200069309503	28.22980226163513	29.045220325837178	22.66977734321819
39	21.489246712955516	25.193592171326497	29.13288859734891	24.18427251836908
40	21.451704064919902	24.959260038532786	30.726388356003316	22.862647540544
41	19.24576407539884	26.182902830550642	29.77420778824296	24.797125305807562
42	19.98217755609738	26.60144147266194	29.97120355128697	23.445177419953712
43	21.1740534920851	25.60965547400687	29.87672809634021	23.339562937567816
44	21.18993692010017	25.793449426752645	28.958789724040905	24.057823929106277
45	20.63484205271648	25.1605876455809	29.341642222689785	24.862928079012836
46	20.902178711255782	25.68618471807947	29.915302135805373	23.49633443485938
47	19.788069689056112	25.76952114558709	31.438460998964484	23.003948166392316
48	20.07562161961459	25.774265546163015	30.185114133775592	23.9649987004468
49	20.16122710826722	25.132327520411234	31.10985968950992	23.596585681811618
50	19.84108320853497	25.013511227727104	31.111097359225383	24.034308204512545
51	19.766410469035566	24.33258660593834	29.82618991629227	26.07481300873382
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186.0
1	472.0
2	758.0
3	6936.5
4	13115.0
5	9242.5
6	5370.0
7	4944.0
8	4518.0
9	4362.5
10	4207.0
11	4063.0
12	3919.0
13	3751.5
14	3584.0
15	3344.5
16	3105.0
17	2948.0
18	2791.0
19	2684.5
20	2578.0
21	2972.0
22	3366.0
23	3273.5
24	3181.0
25	3240.5
26	3819.0
27	4338.0
28	5075.5
29	5813.0
30	6633.5
31	7454.0
32	8458.5
33	9463.0
34	11026.0
35	12589.0
36	13327.0
37	14065.0
38	15131.0
39	16197.0
40	18231.0
41	20265.0
42	22391.5
43	24518.0
44	27210.0
45	29902.0
46	33447.5
47	36993.0
48	39320.0
49	41647.0
50	40810.0
51	39973.0
52	35989.0
53	32005.0
54	28971.0
55	25937.0
56	24149.5
57	22362.0
58	20769.0
59	19176.0
60	18741.0
61	18306.0
62	16387.5
63	14469.0
64	12700.0
65	10931.0
66	9541.0
67	8151.0
68	6691.0
69	5231.0
70	4397.5
71	3564.0
72	3208.0
73	2852.0
74	2425.5
75	1587.0
76	1175.0
77	880.0
78	585.0
79	498.5
80	412.0
81	287.5
82	163.0
83	141.5
84	120.0
85	90.5
86	61.0
87	50.5
88	40.0
89	27.0
90	14.0
91	14.0
92	14.0
93	15.0
94	16.0
95	10.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	484782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.792353943917426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77534522255431	28.247456755946683
2	7.773724943325165	5.720272791549578
3	2.8083898869031643	3.0998182419437836
4	1.5746050642019318	2.3173370737600916
5	1.1363602325947217	2.090468394270867
6	0.9351752859639696	2.064438007247434
7	0.8059401299823555	2.075670416394235
8	0.6646845272742768	1.9564246710816504
9	0.6434326372509536	2.1306061195924784
>10	6.786542760639515	44.7849466910359
>50	0.06785784409431286	1.6524045348661471
>100	0.025660529279361993	1.6991904936364808
>500	0.001140467967971644	0.29833916310051556
>1k	5.70233983985822E-4	0.2240690760839315
>5k	5.70233983985822E-4	1.6385575694901733
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7810	1.6110334129567516	No Hit
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	1068	0.22030520935183237	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	750	0.15470871443246653	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	672	0.13861900813149003	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16564146358569418	0.0
2	0.0	0.0	0.0	0.7925211744660486	0.0
3	0.0	0.0	0.0	1.050162753567583	0.0
4	0.0	0.0	0.0	1.400835839614507	0.0
5	0.0	0.0	0.0	2.3887025508372837	0.0
6	0.0	0.0	0.0	2.9572055068051206	0.0
7	0.0	0.0	0.0	3.4988922856046636	0.0
8	0.0	0.0	0.0	4.410436031040756	0.0
9	0.0	0.0	0.0	4.770597918239538	0.0
10	0.0	0.0	0.0	5.573433007001085	0.0
11	0.0	0.0	0.0	6.488277205011737	0.0
12	0.0	0.0	0.0	7.236861104578965	0.0
13	0.0	0.0	0.0	7.546278533443899	0.0
14	0.0	0.0	0.0	7.663238321554843	0.0
15	0.0	0.0	0.0	7.916341778366358	0.0
16	0.0	0.0	0.0	8.437400728574906	0.0
17	0.0	0.0	0.0	9.050253516013383	0.0
18	0.0	0.0	0.0	9.748505513818582	0.0
19	0.0	0.0	0.0	10.123313159316972	0.0
20	0.0	0.0	0.0	10.50265892710538	0.0
21	0.0	0.0	0.0	10.958533938966381	0.0
22	0.0	0.0	0.0	11.391718339377286	0.0
23	0.0	0.0	0.0	11.878122537552962	0.0
24	0.0	0.0	0.0	12.25849969677092	0.0
25	0.0	0.0	0.0	12.609379061103754	0.0
26	0.0	0.0	0.0	12.918177655110958	0.0
27	0.0	0.0	0.0	13.22718252740407	0.0
28	0.0	0.0	0.0	13.569398203728687	0.0
29	0.0	0.0	0.0	13.916977115486961	0.0
30	0.0	0.0	0.0	14.314062815863625	0.0
31	0.0	0.0	0.0	14.66700496305556	0.0
32	0.0	0.0	0.0	15.012521091954735	0.0
33	0.0	0.0	0.0	15.350817480847061	0.0
34	0.0	0.0	0.0	15.68498830402119	0.0
35	0.0	0.0	0.0	16.037105338069484	0.0
36	0.0	0.0	0.0	16.386128197829127	0.0
37	0.0	0.0	0.0	16.724630865007363	0.0
38	0.0	0.0	0.0	17.059420523039222	0.0
39	0.0	0.0	0.0	17.402461312507477	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.029245E-4	45.000004	1
TCGTTTA	25	3.887456E-5	45.000004	38
TCGCAAG	35	1.2099918E-7	45.000004	1
CCGATGT	25	3.887456E-5	45.000004	11
CGTGCAA	20	7.029245E-4	45.000004	10
GACACGA	145	0.0	45.000004	25
ACATGCG	20	7.029245E-4	45.000004	1
CATCGTT	25	3.887456E-5	45.000004	36
TTCGGTT	20	7.029245E-4	45.000004	16
ATTGCGG	50	2.1827873E-11	45.000004	2
CGTTTAT	25	3.887456E-5	45.000004	39
AATACGG	25	3.887456E-5	45.000004	2
TAGTCGA	20	7.029245E-4	45.000004	44
CGTTTTT	4165	0.0	43.7575	1
CACGTGA	155	0.0	42.09677	43
AAACACG	150	0.0	42.0	40
CGTAAGG	60	3.6379788E-12	41.250004	2
GCGAGAC	165	0.0	40.90909	21
TACGAGC	45	1.9243089E-8	40.000004	34
AACACGT	165	0.0	39.545452	41
>>END_MODULE