##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552334_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	381335
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.42908728545767	28.0	16.0	31.0	16.0	33.0
2	26.703649022513012	30.0	25.0	33.0	16.0	33.0
3	26.94004222009519	30.0	25.0	33.0	16.0	34.0
4	30.571791207206264	33.0	28.0	35.0	19.0	37.0
5	32.368547340265124	33.0	32.0	35.0	28.0	37.0
6	30.982925773925814	35.0	28.0	35.0	17.0	37.0
7	33.21707160370803	35.0	32.0	35.0	28.0	37.0
8	34.01281812579491	35.0	35.0	37.0	31.0	37.0
9	35.411782291161316	37.0	34.0	39.0	30.0	39.0
10	35.40358477454207	37.0	34.0	39.0	31.0	39.0
11	35.737742404972	37.0	35.0	39.0	31.0	39.0
12	35.45508542357769	37.0	34.0	39.0	30.0	39.0
13	35.74712523109602	37.0	35.0	39.0	31.0	39.0
14	36.004208897688386	38.0	34.0	40.0	29.0	41.0
15	36.41525692632462	38.0	34.0	40.0	31.0	41.0
16	36.00416431746365	38.0	34.0	40.0	29.0	41.0
17	36.0865092372848	38.0	34.0	40.0	30.0	41.0
18	35.758933745918945	38.0	34.0	39.0	28.0	40.0
19	35.07343936433844	37.0	33.0	40.0	27.0	41.0
20	35.88496728598214	37.0	34.0	39.0	31.0	40.0
21	35.6414464971744	37.0	34.0	39.0	30.0	40.0
22	36.348706517891095	38.0	35.0	40.0	31.0	41.0
23	36.89311760001049	38.0	35.0	40.0	32.0	41.0
24	36.930753799152974	39.0	35.0	40.0	32.0	41.0
25	34.7935804476379	37.0	33.0	40.0	25.0	41.0
26	35.65624713178701	37.0	34.0	40.0	30.0	41.0
27	36.37834187787641	38.0	35.0	40.0	31.0	41.0
28	36.531199601400346	38.0	35.0	40.0	31.0	41.0
29	36.666678904375416	38.0	35.0	40.0	31.0	41.0
30	35.760158390916125	38.0	35.0	40.0	29.0	41.0
31	35.773264976988735	38.0	35.0	40.0	30.0	41.0
32	35.75407974615496	38.0	35.0	40.0	30.0	41.0
33	35.89272424508634	38.0	35.0	40.0	30.0	41.0
34	35.78249046114309	39.0	35.0	40.0	29.0	41.0
35	35.67337118281826	39.0	35.0	40.0	27.0	41.0
36	35.440072377306045	39.0	35.0	40.0	25.0	41.0
37	35.46843064497096	39.0	35.0	40.0	26.0	41.0
38	35.075193727300146	38.0	35.0	40.0	24.0	41.0
39	35.10016389788506	38.0	35.0	40.0	24.0	41.0
40	34.86550670670146	38.0	34.0	40.0	23.0	41.0
41	34.75162783379443	38.0	34.0	40.0	23.0	41.0
42	34.976986114571176	38.0	34.0	40.0	23.0	41.0
43	35.02873326602594	38.0	35.0	40.0	23.0	41.0
44	35.025615272660524	38.0	35.0	40.0	23.0	41.0
45	35.002729883173586	38.0	35.0	40.0	23.0	41.0
46	34.86230479761889	38.0	34.0	40.0	23.0	41.0
47	34.88703633288316	38.0	34.0	40.0	23.0	41.0
48	34.841994571702045	38.0	34.0	40.0	23.0	41.0
49	34.867245335466194	38.0	34.0	40.0	24.0	41.0
50	34.82099990821718	38.0	34.0	40.0	24.0	41.0
51	33.73691111489897	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	15.0
9	33.0
10	35.0
11	31.0
12	39.0
13	51.0
14	63.0
15	67.0
16	101.0
17	205.0
18	310.0
19	490.0
20	780.0
21	1150.0
22	1712.0
23	2616.0
24	3877.0
25	5800.0
26	7328.0
27	8271.0
28	8094.0
29	8664.0
30	10138.0
31	13114.0
32	18229.0
33	25183.0
34	31997.0
35	44294.0
36	60683.0
37	72696.0
38	48457.0
39	6808.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.120812409036674	19.65122530058872	22.036791797238646	20.191170493135957
2	38.412419526138436	19.660403582152174	23.257503245178125	18.669673646531265
3	31.23028308442708	18.737068456868634	32.83176209894187	17.200886359762414
4	27.376453774240495	24.12786657400973	29.708261764590187	18.787417887159584
5	22.998151231856504	28.054073190239553	30.24558459097644	18.702190986927505
6	23.89814729830726	30.478450706072092	30.620058478765387	15.003343516855258
7	73.58595460684175	6.001547196034982	16.07064654437699	4.341851652746273
8	75.17353508070332	4.388529770411843	15.610159046507663	4.827776102377175
9	69.13422581195012	6.731089462021582	17.715132363931975	6.41955236209632
10	35.367852413232455	24.4577602370619	25.375063920175172	14.799323429530464
11	27.295684896482097	24.81833558419762	30.47058360758913	17.415395911731153
12	24.410033172931936	22.47866049536497	34.52109037984974	18.590215951853356
13	22.363538620897636	23.919912937443456	35.41321934781754	18.303329093841374
14	19.912150733606936	24.82489149960009	35.309898121074646	19.953059645718334
15	19.32106940092045	24.056800451046982	37.372913579923164	19.249216568109407
16	21.41450430723642	23.494040672899157	36.10840861709521	18.98304640276922
17	21.224382760564858	22.771316558931122	34.18070725215362	21.823593428350403
18	22.94649061848506	22.58355514180445	35.03061612492952	19.439338114780966
19	22.631806679166612	24.30959654896613	33.14933064103741	19.90926613082985
20	23.17961897019681	24.523581627702676	33.48132219701837	18.815477205082146
21	22.996577812159913	24.316676937600796	33.3942596404736	19.29248560976569
22	21.54168906604429	22.950686404342637	33.21803663445527	22.2895878951578
23	20.560924121835132	24.96807269198998	33.6376152202132	20.833387965961688
24	21.20969751006333	23.33538752015944	34.896613214103084	20.558301755674144
25	20.836010332122672	23.188272778528066	33.60509787981696	22.370619009532298
26	19.43435561907509	25.790971193307723	33.9006385461602	20.874034641456987
27	20.73164015891539	25.016586465968242	33.9617396777112	20.290033697405168
28	18.667575753602474	25.114400723773063	34.68341484521484	21.53460867740963
29	19.838986717715397	24.605661688541574	33.976687164828824	21.57866442891421
30	21.128141922456635	24.284684070436754	33.54845477073964	21.038719236366973
31	21.395361034261214	25.595867150930285	31.308429596024496	21.70034221878401
32	20.244404526203994	26.400146852505014	31.72171450299605	21.633734118294935
33	20.10515688305558	25.889047687728638	30.17478070462979	23.831014724585994
34	19.391086577418807	25.723314146354255	31.342520356117326	23.543078920109615
35	19.916608756080613	26.552244089842265	30.60956901412144	22.92157813995568
36	20.990205462388715	27.568935450456948	29.062897452371274	22.377961634783063
37	20.493004838265566	28.02863623847798	29.332476693720743	22.14588222953571
38	20.116433057547827	28.414386300759176	29.042705232931677	22.426475408761327
39	21.6413389801618	25.228998125008196	28.761849817089963	24.367813077740045
40	21.571321803663444	24.924541413717595	30.834568030734133	22.669568751884825
41	19.228237638821508	26.24490277577458	29.89104068601099	24.635818899392923
42	20.02281458560059	26.66421912491641	29.797946687295944	23.515019602187053
43	21.118176931044882	25.550500216345206	30.054414097840482	23.27690875476943
44	21.297284539840298	25.754782540286097	28.980292918300183	23.96764000157342
45	20.655329303630666	25.224802339150614	29.350833256847654	24.769035100371063
46	20.742391860175434	25.847876539001142	30.127840350348116	23.281891250475304
47	19.808042797015748	25.94883763619914	31.16891971625998	23.07419985052513
48	19.91739546592891	25.88878545111254	30.300654280357165	23.893164802601387
49	20.294491719878845	25.065624713178703	31.1243394915232	23.51554407541925
50	19.73251865157932	24.944995869773294	30.938413730709218	24.384071747938165
51	19.558918011721975	24.44176380347988	30.007211506942717	25.992106677855432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	316.5
2	501.0
3	5411.5
4	10322.0
5	7068.0
6	3814.0
7	3805.5
8	3797.0
9	3642.0
10	3487.0
11	3439.0
12	3391.0
13	3232.0
14	3073.0
15	2909.0
16	2745.0
17	2510.5
18	2276.0
19	2189.5
20	2103.0
21	2416.5
22	2730.0
23	2673.5
24	2617.0
25	2611.5
26	3024.5
27	3443.0
28	4012.0
29	4581.0
30	5155.5
31	5730.0
32	6473.0
33	7216.0
34	8228.5
35	9241.0
36	10056.0
37	10871.0
38	11763.5
39	12656.0
40	14142.5
41	15629.0
42	17191.5
43	18754.0
44	20467.0
45	22180.0
46	25154.0
47	28128.0
48	30498.0
49	32868.0
50	32252.0
51	31636.0
52	28550.5
53	25465.0
54	23185.5
55	20906.0
56	19458.0
57	18010.0
58	16586.5
59	15163.0
60	14894.0
61	14625.0
62	13178.0
63	11731.0
64	10244.0
65	8757.0
66	7822.0
67	6887.0
68	5613.0
69	4339.0
70	3622.5
71	2906.0
72	2679.0
73	2452.0
74	2041.0
75	1287.5
76	945.0
77	699.5
78	454.0
79	389.5
80	325.0
81	211.0
82	97.0
83	78.0
84	59.0
85	43.5
86	28.0
87	26.0
88	24.0
89	13.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	381335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.81294179760037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82658021079567	30.188792246434836
2	7.974455853506362	6.349730935263648
3	2.968616245321715	3.54568037383213
4	1.848058456477124	2.9430657506514764
5	1.4095566179049617	2.805929779453633
6	1.1462023189785857	2.73802117282614
7	1.0077288186875981	2.8084454164321744
8	0.9261565224894149	2.9498412580271225
9	0.8454946670160983	3.029546697128408
>10	5.976998730565319	37.752774564825415
>50	0.048311922195975944	1.3287543344785258
>100	0.01919240744771647	1.3350779720013264
>500	0.0019854214601086003	0.6094405662599305
>1k	0.0	0.0
>5k	6.618071533695335E-4	1.6148989323852634
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6129	1.6072482200689682	No Hit
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	949	0.24886254867767188	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	746	0.19562851560963457	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	618	0.16206222874900023	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	412	0.10804148583266682	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16652025122267822	0.0
2	0.0	0.0	0.0	0.8142446929864817	0.0
3	0.0	0.0	0.0	1.0667785542895354	0.0
4	0.0	0.0	0.0	1.445185991319968	0.0
5	0.0	0.0	0.0	2.3609162547366487	0.0
6	0.0	0.0	0.0	2.933903260912321	0.0
7	0.0	0.0	0.0	3.4937784362830584	0.0
8	0.0	0.0	0.0	4.3730578100620185	0.0
9	0.0	0.0	0.0	4.74569604153828	0.0
10	0.0	0.0	0.0	5.526636684280226	0.0
11	0.0	0.0	0.0	6.536247656260244	0.0
12	0.0	0.0	0.0	7.296209369714293	0.0
13	0.0	0.0	0.0	7.5891276698965475	0.0
14	0.0	0.0	0.0	7.713952299159532	0.0
15	0.0	0.0	0.0	7.964912740765993	0.0
16	0.0	0.0	0.0	8.482043347712642	0.0
17	0.0	0.0	0.0	9.088072167516751	0.0
18	0.0	0.0	0.0	9.80345365623402	0.0
19	0.0	0.0	0.0	10.181074383416156	0.0
20	0.0	0.0	0.0	10.571806941403228	0.0
21	0.0	0.0	0.0	11.050651002399466	0.0
22	0.0	0.0	0.0	11.522676911377136	0.0
23	0.0	0.0	0.0	11.980804279701575	0.0
24	0.0	0.0	0.0	12.383337485413088	0.0
25	0.0	0.0	0.0	12.740241519923426	0.0
26	0.0	0.0	0.0	13.05544993247407	0.0
27	0.0	0.0	0.0	13.39006385461602	0.0
28	0.0	0.0	0.0	13.733331585089227	0.0
29	0.0	0.0	0.0	14.060340645364311	0.0
30	2.6223661609870585E-4	0.0	0.0	14.473625552335873	0.0
31	2.6223661609870585E-4	0.0	0.0	14.805354871700736	0.0
32	2.6223661609870585E-4	0.0	0.0	15.159898776666186	0.0
33	2.6223661609870585E-4	0.0	0.0	15.542501999554197	0.0
34	2.6223661609870585E-4	0.0	0.0	15.892325645429871	0.0
35	2.6223661609870585E-4	0.0	0.0	16.262865983977342	0.0
36	2.6223661609870585E-4	0.0	0.0	16.61032950030813	0.0
37	2.6223661609870585E-4	0.0	0.0	16.94468118583398	0.0
38	2.6223661609870585E-4	0.0	0.0	17.28689996984279	0.0
39	2.6223661609870585E-4	0.0	0.0	17.65193333945219	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	35	1.209064E-7	45.000004	1
CGCGGGT	35	1.209064E-7	45.000004	4
GTGCGAG	35	1.209064E-7	45.000004	1
TCGTTCA	20	7.0273504E-4	45.0	16
CGACGGT	20	7.0273504E-4	45.0	28
CCGGATG	20	7.0273504E-4	45.0	1
CGTTCAT	25	3.8858852E-5	45.0	17
CGTGTAC	20	7.0273504E-4	45.0	18
TGTAGCG	25	3.8858852E-5	45.0	1
CGGTCTA	20	7.0273504E-4	45.0	31
ACGGTTA	20	7.0273504E-4	45.0	35
TGACCGG	30	2.1616725E-6	44.999996	2
CGTTTTT	3685	0.0	43.59566	1
GCGAGAC	175	0.0	41.142857	21
CACGACC	185	0.0	40.135136	27
TGAATAG	45	1.9224899E-8	40.0	1
CCAGTAG	110	0.0	38.863632	26
ACACGTG	180	0.0	38.75	42
CCTTATC	35	6.237311E-6	38.57143	43
TCCATCG	35	6.237311E-6	38.57143	30
>>END_MODULE