FastQCFastQC Report
Sat 18 Jun 2016
SRR3552329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432215
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87522.0249181541593884No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC33440.7736890205106255No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG32610.7544856147981907No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC28690.6637900119153662No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC17670.40882431197436464TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC6310.14599215668128132TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT6070.14043936466804716No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT6040.13974526566639286No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT5520.12771421630438556TruSeq Adapter, Index 20 (95% over 23bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA5460.126326018301077No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC5340.12354962229445994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGG406.7975634E-945.0000042
CACGACG207.028395E-445.00000426
GCGTTAG207.028395E-445.0000041
TACGCTA207.028395E-445.0000041
TGCGATG207.028395E-445.0000041
GGACGTA207.028395E-445.0000048
GCTAACG406.7975634E-945.0000041
TGTAACG207.028395E-445.0000041
ACGAAAG207.028395E-445.0000041
CAAACGC253.886753E-545.034
CGTTAGG351.2095734E-745.02
CTAACGG551.8189894E-1245.02
TGCGACG253.886753E-545.01
AAATTCG253.886753E-545.030
GCGATAA253.886753E-545.09
TCGTACA253.886753E-545.034
AGTACGG351.2095734E-745.02
CGTTTTT43550.042.881751
AATGGGC750.042.04
AATGCGG501.0786607E-940.52