##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432215
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.881880545561817	30.0	25.0	31.0	16.0	33.0
2	28.560739446803094	31.0	28.0	33.0	16.0	33.0
3	28.2083500109899	31.0	26.0	33.0	16.0	34.0
4	32.23455918929237	35.0	32.0	35.0	25.0	37.0
5	33.35184341126523	35.0	32.0	35.0	28.0	37.0
6	33.18414215147554	35.0	33.0	35.0	28.0	37.0
7	34.14181599435466	35.0	33.0	36.0	31.0	37.0
8	34.30359658966024	35.0	35.0	37.0	31.0	37.0
9	35.586682553821596	37.0	35.0	39.0	30.0	39.0
10	35.52778593986789	37.0	34.0	39.0	30.0	39.0
11	35.783422602177154	37.0	35.0	39.0	31.0	39.0
12	35.69727103409183	37.0	35.0	39.0	30.0	39.0
13	35.854801429843945	37.0	35.0	39.0	31.0	39.0
14	36.85872308920329	38.0	36.0	40.0	31.0	41.0
15	37.12447277396666	39.0	36.0	40.0	32.0	41.0
16	36.79267956919588	38.0	35.0	40.0	31.0	41.0
17	35.86416482537626	38.0	34.0	40.0	29.0	41.0
18	35.65881100841017	38.0	34.0	39.0	29.0	41.0
19	35.0558472056731	37.0	33.0	39.0	27.0	40.0
20	35.63622039956966	37.0	34.0	39.0	30.0	40.0
21	35.91476464259686	38.0	34.0	39.0	30.0	40.0
22	36.32839211966267	38.0	35.0	40.0	31.0	41.0
23	36.56100089076038	38.0	35.0	40.0	31.0	41.0
24	36.477438311951225	38.0	35.0	40.0	31.0	41.0
25	35.729861295882834	38.0	34.0	40.0	29.0	41.0
26	35.930863112108554	38.0	35.0	40.0	30.0	41.0
27	36.219203405712435	38.0	35.0	40.0	31.0	41.0
28	36.157555846048844	38.0	35.0	40.0	30.0	41.0
29	36.20168434691068	38.0	35.0	40.0	30.0	41.0
30	35.656846708235484	38.0	35.0	40.0	29.0	41.0
31	35.770621102923315	38.0	35.0	40.0	29.0	41.0
32	35.57862406441239	38.0	35.0	40.0	29.0	41.0
33	35.45075945999098	38.0	35.0	40.0	27.0	41.0
34	35.225612253161046	38.0	35.0	40.0	25.0	41.0
35	34.947419686961354	38.0	34.0	40.0	23.0	41.0
36	34.730724292308224	38.0	34.0	40.0	23.0	41.0
37	34.63530650255081	38.0	34.0	40.0	22.0	41.0
38	34.486679083326585	38.0	34.0	40.0	22.0	41.0
39	34.26885230730076	38.0	34.0	40.0	21.0	41.0
40	34.19207570306445	38.0	34.0	40.0	21.0	41.0
41	34.0804321923117	38.0	33.0	40.0	20.0	41.0
42	34.003468181344935	37.0	33.0	40.0	19.0	41.0
43	33.94701710954039	37.0	33.0	40.0	19.0	41.0
44	33.82557754821096	37.0	33.0	40.0	18.0	41.0
45	33.80378283955901	37.0	33.0	40.0	20.0	41.0
46	33.555107990236344	37.0	33.0	40.0	19.0	41.0
47	33.562053607579564	37.0	33.0	40.0	20.0	41.0
48	33.48941614705644	36.0	33.0	40.0	20.0	41.0
49	33.44170146801939	36.0	33.0	40.0	19.0	41.0
50	33.28491838552572	36.0	33.0	39.0	18.0	40.0
51	30.82666034265354	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	14.0
9	24.0
10	27.0
11	36.0
12	22.0
13	28.0
14	50.0
15	102.0
16	169.0
17	284.0
18	563.0
19	846.0
20	1200.0
21	1705.0
22	2457.0
23	3516.0
24	4999.0
25	7123.0
26	9803.0
27	10691.0
28	10664.0
29	10830.0
30	12462.0
31	15320.0
32	20242.0
33	27936.0
34	37046.0
35	43929.0
36	56170.0
37	72521.0
38	66352.0
39	15082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.69598463727543	19.020626308665825	21.309302083453836	19.97408697060491
2	37.72497483891119	23.017942459192763	23.00429184549356	16.252790856402484
3	29.690778894763024	22.021447659151118	32.863274064990804	15.424499381095059
4	27.270687042328472	22.327082586212878	32.963455687562906	17.438774683895748
5	23.954513378758257	26.863945027359073	30.79624723806439	18.385294355818285
6	23.45314253322999	32.32002591302939	30.727994169568383	13.49883738417223
7	70.83465404948926	5.9993290376317345	18.922526983098688	4.243489929780318
8	71.64582441608921	6.443783765024351	16.986453501151047	4.923938317735386
9	65.6356211607649	6.988420114989068	18.894994389366403	8.480964334879632
10	36.72015085084969	22.629941117268025	25.66778108117488	14.982126950707404
11	28.59572203648647	22.635725275615144	30.88624874194556	17.882303945952824
12	24.544960262832156	20.75749337713869	35.42820124243721	19.26934511759194
13	21.947179065974108	21.705632613398425	38.1058038244855	18.241384496141965
14	19.60112444038268	25.17150029499208	34.81438635864095	20.41298890598429
15	19.192994227409972	22.52652036602154	39.81166780421781	18.468817602350683
16	21.716969563758777	22.304408685492174	34.76950128986731	21.209120460881735
17	20.2868942540171	21.706095346066192	35.231539858635166	22.77547054128154
18	21.857408928426825	21.936304848281527	35.80046967365779	20.40581654963386
19	22.505466029638026	23.536203047094617	32.44241870365443	21.51591221961292
20	23.032518538227503	23.39044225674722	33.89354834977962	19.68349085524565
21	22.601483058200202	24.376756938097937	34.07100632786923	18.95075367583263
22	21.65496338627766	21.033282047129322	33.04443390442257	24.267320662170448
23	20.789653297548675	24.427194798884813	33.24549124856842	21.537660654998092
24	21.279687192716587	22.063093599250372	34.04532466480802	22.611894543225013
25	20.78942193121479	24.519047233437064	32.80149925384357	21.890031581504577
26	19.884548199391507	22.919380400957856	33.58374882870793	23.612322570942702
27	22.009647976122995	22.88698911421399	33.64159041217912	21.461772497483892
28	18.887822033015976	23.750216905938018	35.25004916534595	22.111911895700057
29	21.22092014390986	22.612357275892787	32.64879747347963	23.517925106717723
30	20.6753583286096	22.9809238457712	34.305843156762265	22.037874668856936
31	22.332172645558344	22.56006848443483	31.101882165126156	24.00587670488067
32	21.7454276228266	25.611327695706997	30.89110743495714	21.75213724650926
33	20.06385710815219	23.12784146778802	30.525779993753112	26.282521430306677
34	20.234374096225256	23.384889464733988	32.325347338708745	24.05538910033201
35	19.716576240991174	24.968129287507374	31.094709808775722	24.220584662725727
36	20.947213770924193	24.192357969991786	29.891142139907224	24.969286119176797
37	20.387538609256968	26.344296241453907	31.574332218918826	21.693832930370302
38	20.466665895445555	26.903045937785592	28.1612160614509	24.469072105317956
39	21.62997582221811	24.216651435049684	30.231713383385582	23.921659359346624
40	21.593882674132086	23.933690408708628	31.204840183704867	23.267586733454415
41	19.421352798954224	24.591696262276876	30.408014529805765	25.57893640896313
42	20.13118471131266	24.967666554839603	31.095172541443496	23.805976192404245
43	21.719514593431512	22.483717594252862	30.09150538505142	25.70526242726421
44	20.908344226831556	23.416818018810083	30.56094767650359	25.113890077854773
45	20.464814964774476	22.990409865460474	30.597503557257383	25.947271612507667
46	22.43119743646102	24.40984232384346	29.979061346783432	23.179898892912092
47	19.89010099140474	23.301134851867705	34.02727809076501	22.781486065962543
48	19.969459643927213	24.615064261999237	30.74025658526428	24.675219508809274
49	19.846141387966636	23.20604328864107	33.565008155663264	23.382807167729027
50	20.24663651192115	22.470761079555313	32.436634545307314	24.845967863216224
51	20.159874136714368	22.522355772011615	30.24166213574263	27.07610795553139
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	164.0
1	487.5
2	811.0
3	7591.5
4	14372.0
5	9999.0
6	5626.0
7	5064.5
8	4503.0
9	4251.0
10	3999.0
11	3778.0
12	3557.0
13	3420.0
14	3283.0
15	2996.5
16	2710.0
17	2518.0
18	2326.0
19	2185.0
20	2044.0
21	2505.0
22	2966.0
23	2746.5
24	2527.0
25	2703.0
26	3067.0
27	3255.0
28	3768.0
29	4281.0
30	5040.0
31	5799.0
32	6308.5
33	6818.0
34	7637.5
35	8457.0
36	9429.5
37	10402.0
38	11006.5
39	11611.0
40	12976.0
41	14341.0
42	16417.5
43	18494.0
44	21312.5
45	24131.0
46	32578.5
47	41026.0
48	38488.0
49	35950.0
50	35155.0
51	34360.0
52	30585.0
53	26810.0
54	24391.0
55	21972.0
56	21136.0
57	20300.0
58	19428.0
59	18556.0
60	17696.0
61	16836.0
62	15870.0
63	14904.0
64	13212.5
65	11521.0
66	10032.0
67	8543.0
68	7577.0
69	6611.0
70	5841.0
71	5071.0
72	4279.5
73	3488.0
74	3139.0
75	2269.0
76	1748.0
77	1387.5
78	1027.0
79	851.0
80	675.0
81	533.0
82	391.0
83	267.5
84	144.0
85	107.0
86	70.0
87	48.0
88	26.0
89	26.0
90	26.0
91	16.0
92	6.0
93	5.0
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.4299383349692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18146053343955	28.117158478360615
2	7.752623990933098	5.648552278477911
3	2.6712002533081862	2.9193498152511395
4	1.5079699037415037	2.1974100241716967
5	1.1434737213854163	2.082833857886423
6	0.8920291771260315	1.9497940749416787
7	0.7732823271390958	1.9719439245239119
8	0.7066731727389696	2.0595248084686157
9	0.632500570609626	2.0737761105727355
>10	6.631600134605565	41.93113558562955
>50	0.07296411019240219	1.7976134817061755
>100	0.027119403788326477	1.730808688614766
>500	0.003874200541189497	0.8171825746463299
>1k	0.002582800360792998	2.6441995744385136
>5k	6.457000901982495E-4	2.0587167223099256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8752	2.0249181541593884	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	3344	0.7736890205106255	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	3261	0.7544856147981907	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	2869	0.6637900119153662	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	1767	0.40882431197436464	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	631	0.14599215668128132	TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	607	0.14043936466804716	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	604	0.13974526566639286	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	552	0.12771421630438556	TruSeq Adapter, Index 20 (95% over 23bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	546	0.126326018301077	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC	534	0.12354962229445994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15362724569947828	0.0
2	0.0	0.0	0.0	0.9798364240019435	0.0
3	0.0	0.0	0.0	1.235958955612369	0.0
4	0.0	0.0	0.0	1.7074835440695024	0.0
5	0.0	0.0	0.0	3.460546255914302	0.0
6	0.0	0.0	0.0	4.056777298335319	0.0
7	0.0	0.0	0.0	4.774938398713603	0.0
8	0.0	0.0	0.0	5.839223534583483	0.0
9	0.0	0.0	0.0	6.233471767523108	0.0
10	0.0	0.0	0.0	7.765116897840195	0.0
11	0.0	0.0	0.0	8.675774788010596	0.0
12	0.0	0.0	0.0	10.13083766181183	0.0
13	0.0	0.0	0.0	10.479969459643927	0.0
14	0.0	0.0	0.0	10.62804391333017	0.0
15	0.0	0.0	0.0	10.957972305449834	0.0
16	0.0	0.0	0.0	11.45008849762271	0.0
17	0.0	0.0	0.0	12.037064886688338	0.0
18	0.0	0.0	0.0	12.707795888620247	0.0
19	0.0	0.0	0.0	13.0849230128524	0.0
20	0.0	0.0	0.0	13.446085860046505	0.0
21	0.0	0.0	0.0	13.852133776014252	0.0
22	0.0	0.0	0.0	14.274608701687818	0.0
23	0.0	0.0	0.0	14.68088798398945	0.0
24	0.0	0.0	0.0	15.038580336175283	0.0
25	0.0	0.0	0.0	15.356246312599053	0.0
26	0.0	0.0	0.0	15.638975972606225	0.0
27	0.0	0.0	0.0	15.9247133949539	0.0
28	0.0	0.0	0.0	16.21507814397927	0.0
29	0.0	0.0	0.0	16.526728595722037	0.0
30	0.0	0.0	0.0	16.885346413243408	0.0
31	0.0	0.0	0.0	17.219439399372998	0.0
32	0.0	0.0	0.0	17.54797959348935	0.0
33	0.0	0.0	0.0	17.884617609291674	0.0
34	0.0	0.0	0.0	18.196036694700553	0.0
35	0.0	0.0	0.0	18.531749245167337	0.0
36	0.0	0.0	0.0	18.849877954258876	0.0
37	0.0	0.0	0.0	19.17633585137027	0.0
38	0.0	0.0	0.0	19.47017109540391	0.0
39	0.0	0.0	0.0	19.798479923186378	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGG	40	6.7975634E-9	45.000004	2
CACGACG	20	7.028395E-4	45.000004	26
GCGTTAG	20	7.028395E-4	45.000004	1
TACGCTA	20	7.028395E-4	45.000004	1
TGCGATG	20	7.028395E-4	45.000004	1
GGACGTA	20	7.028395E-4	45.000004	8
GCTAACG	40	6.7975634E-9	45.000004	1
TGTAACG	20	7.028395E-4	45.000004	1
ACGAAAG	20	7.028395E-4	45.000004	1
CAAACGC	25	3.886753E-5	45.0	34
CGTTAGG	35	1.2095734E-7	45.0	2
CTAACGG	55	1.8189894E-12	45.0	2
TGCGACG	25	3.886753E-5	45.0	1
AAATTCG	25	3.886753E-5	45.0	30
GCGATAA	25	3.886753E-5	45.0	9
TCGTACA	25	3.886753E-5	45.0	34
AGTACGG	35	1.2095734E-7	45.0	2
CGTTTTT	4355	0.0	42.88175	1
AATGGGC	75	0.0	42.0	4
AATGCGG	50	1.0786607E-9	40.5	2
>>END_MODULE