SRR3552325_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	349911
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	14922	4.264512976156794	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	14736	4.211356602107394	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC	12264	3.504891243773417	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8345	2.38489215829168	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	4272	1.2208818813926972	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	2766	0.7904867237669008	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	2344	0.6698846278053563	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC	2106	0.6018673319787031	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	1870	0.5344216100665597	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA	1454	0.4155342358485444	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	611	0.17461583088271018	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTT	547	0.1563254656184001	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	533	0.15232444821683228	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTAT	421	0.12031630900428966	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.10917061767135071	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGT	363	0.10374066548350866	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	25	3.8852264E-5	45.0	6
ATGATCC	45	3.8380676E-10	45.0	3
ACGCACA	25	3.8852264E-5	45.0	32
CCTACGC	25	3.8852264E-5	45.0	29
CGATTAT	20	7.026554E-4	45.0	10
CCTCGTC	20	7.026554E-4	45.0	36
ACGGGTC	20	7.026554E-4	45.0	5
ACGGGTA	20	7.026554E-4	45.0	5
TACGCAC	25	3.8852264E-5	45.0	31
CTACGCA	25	3.8852264E-5	45.0	30
TCGTCCC	20	7.026554E-4	45.0	38
AATTAGA	20	7.026554E-4	45.0	41
TGTAGAG	25	3.8852264E-5	45.0	1
ATACGTC	20	7.026554E-4	45.0	40
TGTCGCG	20	7.026554E-4	45.0	1
CAGTAGC	30	2.1611577E-6	44.999996	27
TGATACC	1590	0.0	44.292454	4
TACGGCT	1465	0.0	44.0785	7
CGTTTTT	4420	0.0	43.930992	1
GATACCT	1610	0.0	43.742233	5