##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	349911
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.11922746069715	30.0	28.0	31.0	16.0	33.0
2	28.686234499629904	31.0	28.0	31.0	16.0	33.0
3	28.223525410747303	31.0	27.0	31.0	16.0	34.0
4	32.047837878774885	35.0	32.0	35.0	25.0	37.0
5	33.24193009079452	35.0	32.0	35.0	28.0	37.0
6	33.275904444272975	35.0	33.0	35.0	28.0	37.0
7	34.022682910797315	35.0	33.0	35.0	31.0	37.0
8	33.79209284646667	35.0	33.0	37.0	28.0	37.0
9	35.164750465118274	37.0	34.0	39.0	29.0	39.0
10	35.32506265879038	37.0	34.0	39.0	30.0	39.0
11	35.676592047692125	37.0	35.0	39.0	30.0	39.0
12	35.61955468676321	37.0	35.0	39.0	30.0	39.0
13	35.395380539622934	37.0	34.0	39.0	30.0	39.0
14	36.74606399913121	38.0	35.0	40.0	31.0	41.0
15	36.49021608351838	38.0	35.0	40.0	30.0	41.0
16	36.51966071372435	38.0	35.0	40.0	31.0	41.0
17	35.64992240884111	37.0	33.0	40.0	29.0	41.0
18	35.21316277567724	37.0	33.0	39.0	27.0	40.0
19	34.63470425336728	37.0	33.0	39.0	27.0	40.0
20	34.98918010579833	36.0	34.0	39.0	27.0	40.0
21	35.59107315860319	37.0	34.0	39.0	30.0	40.0
22	36.03319129721558	37.0	35.0	40.0	31.0	40.0
23	36.227100605582564	38.0	35.0	40.0	31.0	41.0
24	36.12520612384292	38.0	35.0	40.0	31.0	41.0
25	35.52245285229672	37.0	34.0	40.0	29.0	41.0
26	35.61162124082981	37.0	34.0	40.0	30.0	41.0
27	35.97142987788323	38.0	35.0	40.0	31.0	41.0
28	35.85385140792945	38.0	35.0	40.0	30.0	41.0
29	35.770475921019916	38.0	35.0	40.0	30.0	41.0
30	35.32251915487081	37.0	34.0	40.0	28.0	41.0
31	35.318369528251466	37.0	34.0	40.0	28.0	41.0
32	34.83786734341018	37.0	34.0	40.0	25.0	41.0
33	34.62148374872468	37.0	34.0	40.0	24.0	41.0
34	34.14075865005673	37.0	33.0	40.0	21.0	41.0
35	33.91991963670762	37.0	33.0	40.0	18.0	41.0
36	33.76560039552915	37.0	33.0	40.0	18.0	41.0
37	33.49449145639891	37.0	33.0	40.0	16.0	41.0
38	33.52300442112423	37.0	33.0	40.0	17.0	41.0
39	32.9369639708383	37.0	32.0	40.0	15.0	41.0
40	33.01258319972793	37.0	32.0	40.0	15.0	41.0
41	32.59871224397061	36.0	31.0	40.0	12.0	41.0
42	32.89768826930277	37.0	32.0	40.0	15.0	41.0
43	32.919273758184225	37.0	32.0	40.0	15.0	40.0
44	32.89042928058849	37.0	32.0	40.0	14.0	40.0
45	32.83581539305709	36.0	32.0	40.0	15.0	40.0
46	32.502333450505986	36.0	31.0	39.0	12.0	40.0
47	32.346779609672176	36.0	31.0	39.0	12.0	40.0
48	32.331775794416295	35.0	31.0	39.0	12.0	40.0
49	32.30245691047152	35.0	31.0	39.0	12.0	40.0
50	32.13817227809357	35.0	31.0	39.0	11.0	40.0
51	29.187627711046524	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	8.0
10	13.0
11	16.0
12	8.0
13	14.0
14	33.0
15	50.0
16	116.0
17	295.0
18	514.0
19	743.0
20	1119.0
21	1556.0
22	2419.0
23	3462.0
24	5183.0
25	7323.0
26	9585.0
27	10279.0
28	10565.0
29	10484.0
30	12012.0
31	14703.0
32	18882.0
33	25384.0
34	32942.0
35	38400.0
36	45483.0
37	50488.0
38	39536.0
39	8290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.87020413762357	14.898645655609569	17.31354544441301	20.917604762353857
2	35.23010136863374	33.60540251663994	18.010294046200322	13.154202068525997
3	22.94412007624796	33.23159317655061	32.26734798277276	11.556938764428669
4	21.140518589012636	17.17693927884519	47.50436539577207	14.178176736370105
5	29.5266510626988	20.512073070009233	31.570885167942702	18.39039069934926
6	18.4249709211771	35.550182760759164	34.32958666632373	11.695259651740015
7	60.46108867683496	3.810683288036101	31.880106655692448	3.8481213794364852
8	56.61639674088554	13.260514816624799	23.978954648467756	6.144133794021908
9	52.54250366521772	4.541154750779484	22.801512384577794	20.114829199424996
10	33.67113351680856	17.204660613698913	31.725495911817575	17.398709957674953
11	26.95714053002049	18.23492259460263	35.20695262509609	19.60098425028079
12	22.645186918959393	16.043793993329732	40.50830068217346	20.80271840553741
13	17.56903898419884	19.953073781618755	48.08737078857195	14.390516445610455
14	18.479556230012776	24.65484080237546	35.53932285638348	21.326280111228282
15	13.162204103329131	19.88905750319367	50.82635298690238	16.122385406574814
16	16.1718265501799	22.419986796642576	35.106641403099644	26.30154525007788
17	16.16696817190657	20.372609034868866	41.2093360883196	22.251086704904964
18	17.615336471274123	21.534333016109812	39.530051927490135	21.320278585125934
19	17.017755943654244	23.841205335070917	35.01004541154751	24.13099330972733
20	18.70161269579979	22.860098710815034	42.415357047935046	16.022931545450128
21	18.957677809500133	27.54986267936704	36.80793115963773	16.684528351495096
22	17.997147846166598	17.976856972201503	37.136300373523554	26.88969480810835
23	17.003752382748758	27.254644752522783	37.93621806687986	17.805384797848596
24	22.481716779409624	18.23092157720106	33.970066674097126	25.317294969292192
25	16.711678112434306	31.284526636773354	32.63715630546053	19.36663894533181
26	17.06405343072953	19.128578409938527	36.302374032253915	27.50499412707803
27	23.087870915747146	22.23851207878575	31.65490653337563	23.018710472091474
28	16.62422730351432	19.476381136917674	40.17164364652726	23.727747913040744
29	22.16077802641243	18.64931368262215	31.553737950507415	27.636170340458
30	24.888042959495415	22.94354850233345	34.76598335005187	17.402425188119263
31	28.18345236360103	18.625879152127254	31.45799931982704	21.732669164444673
32	24.96577701186873	27.272935117787096	31.09390673628437	16.667381134059802
33	24.755723598286423	20.250292217163793	29.73956234585366	25.25442183869613
34	22.902109393531497	19.537539545770212	36.63217218092601	20.928178879772286
35	22.875816993464053	21.035920562657363	34.22956123128453	21.85870121259406
36	25.68567435719369	26.145219784459478	28.378644855406087	19.79046100294075
37	22.740353975725256	26.096350214768897	34.55935937995662	16.60393642954923
38	27.21949295678044	24.62569053273547	26.402428045988835	21.752388464495258
39	23.147028815898903	25.37559550857218	33.3050404245651	18.172335250963815
40	25.523061578515684	19.4838115978063	37.09657598646513	17.896550837212892
41	18.88337320061387	29.127692470371038	31.206221010485525	20.78271331852957
42	20.054242364486967	20.858732649159357	39.65351189302422	19.433513093329445
43	25.30357719534396	23.95094752665678	27.166336582731038	23.579138695268227
44	23.587426517028614	20.0236631600607	32.15874893901592	24.23016138389476
45	19.635564472108623	18.825072661333884	31.591176041907797	29.948186824649696
46	27.991975102240286	24.099842531386553	28.589841416817418	19.318340949555743
47	17.743940602038805	19.755023420241148	42.73458107918871	19.76645489853134
48	22.856097693413467	22.652903166805274	29.115689418166333	25.375309721614926
49	19.13286521429735	18.12803827258932	39.98159531995279	22.757501193160547
50	22.221364861350455	18.54471565626688	34.838287450237345	24.39563203214532
51	21.52833149000746	18.342092703573197	29.514933797451352	30.614642008967998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	286.0
2	505.0
3	7463.0
4	14421.0
5	9965.5
6	5510.0
7	4980.0
8	4450.0
9	4249.5
10	4049.0
11	3836.5
12	3624.0
13	3446.0
14	3268.0
15	2987.5
16	2707.0
17	2559.5
18	2412.0
19	2197.5
20	1983.0
21	1944.5
22	1906.0
23	1828.0
24	1750.0
25	1710.5
26	1943.0
27	2215.0
28	2483.5
29	2752.0
30	2934.5
31	3117.0
32	3782.0
33	4447.0
34	4838.5
35	5230.0
36	5961.5
37	6693.0
38	7519.5
39	8346.0
40	8723.5
41	9101.0
42	10592.0
43	12083.0
44	14722.0
45	17361.0
46	45105.5
47	72850.0
48	50260.5
49	27671.0
50	27713.5
51	27756.0
52	23838.5
53	19921.0
54	18024.0
55	16127.0
56	14966.5
57	13806.0
58	12660.0
59	11514.0
60	10524.0
61	9534.0
62	8995.5
63	8457.0
64	7793.0
65	7129.0
66	5995.5
67	4862.0
68	4414.0
69	3966.0
70	3240.5
71	2515.0
72	2111.0
73	1707.0
74	1430.0
75	873.0
76	593.0
77	452.0
78	311.0
79	240.0
80	169.0
81	134.5
82	100.0
83	78.5
84	57.0
85	36.5
86	16.0
87	15.5
88	15.0
89	11.0
90	7.0
91	5.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	349911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.358529804793662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20945862099656	23.136071209527135
2	8.597133015202358	5.219926377555928
3	2.8671981468329344	2.6113176119063035
4	1.5797950627108321	1.9184102198709068
5	1.0367955741677615	1.5737794669925074
6	0.8741218486111899	1.5922232516450485
7	0.7823938376029469	1.662662864656917
8	0.6896975757192445	1.6750563527013294
9	0.6867066075369327	1.8762662710852842
>10	6.5321824018197345	34.808192645070385
>50	0.10265712280239912	2.08020100394302
>100	0.02890346175989878	1.6429805747506323
>500	0.0029900132855067703	0.5116538032971122
>1k	0.0059800265710135405	4.481736330240583
>5k	9.966710951689235E-4	2.5249858004224723
>10k+	0.0029900132855067703	12.684536216334438
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	14922	4.264512976156794	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	14736	4.211356602107394	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC	12264	3.504891243773417	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8345	2.38489215829168	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	4272	1.2208818813926972	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	2766	0.7904867237669008	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	2344	0.6698846278053563	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC	2106	0.6018673319787031	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	1870	0.5344216100665597	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA	1454	0.4155342358485444	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	611	0.17461583088271018	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTT	547	0.1563254656184001	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	533	0.15232444821683228	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTAT	421	0.12031630900428966	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.10917061767135071	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGT	363	0.10374066548350866	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.857869572548448E-4	0.0	0.0	0.2163407266419175	0.0
2	2.857869572548448E-4	0.0	0.0	2.064524979208999	0.0
3	2.857869572548448E-4	0.0	0.0	2.371174384343447	0.0
4	2.857869572548448E-4	0.0	0.0	3.445161769707154	0.0
5	2.857869572548448E-4	0.0	0.0	9.151184158257385	0.0
6	2.857869572548448E-4	0.0	0.0	10.182875073947375	0.0
7	2.857869572548448E-4	0.0	0.0	11.465772725064374	0.0
8	2.857869572548448E-4	0.0	0.0	12.688369328200599	0.0
9	2.857869572548448E-4	0.0	0.0	12.93843291579859	0.0
10	2.857869572548448E-4	0.0	0.0	18.013437702730123	0.0
11	2.857869572548448E-4	0.0	0.0	18.761056382908798	0.0
12	2.857869572548448E-4	0.0	0.0	23.265916190116915	0.0
13	2.857869572548448E-4	0.0	0.0	23.640011317163506	0.0
14	2.857869572548448E-4	0.0	0.0	23.882930230830127	0.0
15	2.857869572548448E-4	0.0	0.0	24.58510878480528	0.0
16	2.857869572548448E-4	0.0	0.0	25.09609586437694	0.0
17	2.857869572548448E-4	0.0	0.0	25.633946917930558	0.0
18	2.857869572548448E-4	0.0	0.0	26.10892484088811	0.0
19	2.857869572548448E-4	0.0	0.0	26.515599681061754	0.0
20	2.857869572548448E-4	0.0	0.0	26.81081760790601	0.0
21	2.857869572548448E-4	0.0	0.0	27.196630000200052	0.0
22	2.857869572548448E-4	0.0	0.0	27.63674191437251	0.0
23	2.857869572548448E-4	0.0	0.0	28.030270554512434	0.0
24	2.857869572548448E-4	0.0	0.0	28.34520778140727	0.0
25	2.857869572548448E-4	0.0	0.0	28.627851082132313	0.0
26	2.857869572548448E-4	0.0	0.0	28.913923826344412	0.0
27	2.857869572548448E-4	0.0	0.0	29.230289988025525	0.0
28	2.857869572548448E-4	0.0	0.0	29.502073384374885	0.0
29	2.857869572548448E-4	0.0	0.0	29.770998911151693	0.0
30	5.715739145096896E-4	0.0	0.0	30.090794516319864	0.0
31	5.715739145096896E-4	0.0	0.0	30.376295686617453	0.0
32	5.715739145096896E-4	0.0	0.0	30.673228335205238	0.0
33	5.715739145096896E-4	0.0	0.0	30.951870618528712	0.0
34	5.715739145096896E-4	0.0	0.0	31.233656558381988	0.0
35	5.715739145096896E-4	0.0	0.0	31.554595311379178	0.0
36	5.715739145096896E-4	0.0	0.0	31.80465889897717	0.0
37	5.715739145096896E-4	0.0	0.0	32.08501590404417	0.0
38	5.715739145096896E-4	0.0	0.0	32.34593939601785	0.0
39	5.715739145096896E-4	0.0	0.0	32.665735001186015	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	25	3.8852264E-5	45.0	6
ATGATCC	45	3.8380676E-10	45.0	3
ACGCACA	25	3.8852264E-5	45.0	32
CCTACGC	25	3.8852264E-5	45.0	29
CGATTAT	20	7.026554E-4	45.0	10
CCTCGTC	20	7.026554E-4	45.0	36
ACGGGTC	20	7.026554E-4	45.0	5
ACGGGTA	20	7.026554E-4	45.0	5
TACGCAC	25	3.8852264E-5	45.0	31
CTACGCA	25	3.8852264E-5	45.0	30
TCGTCCC	20	7.026554E-4	45.0	38
AATTAGA	20	7.026554E-4	45.0	41
TGTAGAG	25	3.8852264E-5	45.0	1
ATACGTC	20	7.026554E-4	45.0	40
TGTCGCG	20	7.026554E-4	45.0	1
CAGTAGC	30	2.1611577E-6	44.999996	27
TGATACC	1590	0.0	44.292454	4
TACGGCT	1465	0.0	44.0785	7
CGTTTTT	4420	0.0	43.930992	1
GATACCT	1610	0.0	43.742233	5
>>END_MODULE