SRR3552324_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552324_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290900
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	10170	3.496046751460983	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	10081	3.465452045376418	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC	10026	3.446545204537642	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6809	2.3406668958404953	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3617	1.2433826057064283	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAGTTAT	2160	0.7425232038501204	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	1913	0.657614300446889	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	1913	0.657614300446889	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA	1906	0.6552079752492266	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	1431	0.49192162255070465	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC	1402	0.4819525610175318	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATG	562	0.19319353729804056	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	561	0.19284977655551735	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	534	0.18356823650739085	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGT	450	0.15469233413544173	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTAT	421	0.14472327260226883	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGGAGTTA	366	0.1258164317634926	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	298	0.10244070127191475	TruSeq Adapter, Index 22 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	30	2.1598935E-6	45.000004	17
AACAAGG	30	2.1598935E-6	45.000004	2
TACGGGA	30	2.1598935E-6	45.000004	4
AACGGGA	45	3.8198777E-10	45.000004	4
ACGGCTA	65	0.0	45.000004	30
CGGGCTT	30	2.1598935E-6	45.000004	6
GACGGGC	30	2.1598935E-6	45.000004	4
GCGATAC	30	2.1598935E-6	45.000004	33
CGACTGA	30	2.1598935E-6	45.000004	41
CGTGGGA	20	7.0245925E-4	45.0	4
CGAACCC	25	3.8835995E-5	45.0	34
GCAAGCC	20	7.0245925E-4	45.0	40
GCGACGA	25	3.8835995E-5	45.0	33
GATCGCG	20	7.0245925E-4	45.0	10
GCGCGAT	20	7.0245925E-4	45.0	31
GACCGTT	25	3.8835995E-5	45.0	22
CGCGATT	20	7.0245925E-4	45.0	32
CGACAGT	20	7.0245925E-4	45.0	30
ATCACGG	20	7.0245925E-4	45.0	2
AGACGGT	20	7.0245925E-4	45.0	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content