##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552324_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	290900
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.264156067377105	28.0	25.0	31.0	16.0	33.0
2	27.122093502921967	30.0	25.0	31.0	16.0	33.0
3	27.210553454795463	30.0	25.0	31.0	16.0	33.0
4	30.680873152286008	33.0	28.0	35.0	19.0	37.0
5	32.50631488484015	35.0	32.0	35.0	28.0	37.0
6	31.72743554486078	35.0	32.0	35.0	19.0	37.0
7	33.2322344448264	35.0	32.0	35.0	28.0	37.0
8	32.90050532829151	35.0	32.0	36.0	28.0	37.0
9	34.79368855276727	37.0	32.0	39.0	28.0	39.0
10	35.09339635613613	37.0	34.0	39.0	30.0	39.0
11	35.5929975936748	37.0	35.0	39.0	31.0	39.0
12	35.291323478858715	37.0	34.0	39.0	30.0	39.0
13	35.281113784805775	37.0	34.0	39.0	30.0	39.0
14	35.90126847713991	38.0	34.0	40.0	30.0	41.0
15	36.06936060501891	38.0	34.0	40.0	30.0	41.0
16	35.749529047782744	37.0	34.0	40.0	29.0	41.0
17	35.542327260226884	37.0	34.0	40.0	29.0	41.0
18	35.30677208662771	37.0	34.0	39.0	27.0	40.0
19	34.60079408731523	37.0	32.0	39.0	27.0	40.0
20	34.465097971811616	35.0	33.0	39.0	27.0	40.0
21	34.88172567892747	36.0	33.0	39.0	27.0	40.0
22	35.40205912684771	37.0	34.0	39.0	30.0	40.0
23	35.81138535579237	37.0	34.0	40.0	30.0	40.0
24	35.81153661051908	37.0	34.0	40.0	30.0	41.0
25	34.13102784462014	35.0	33.0	39.0	25.0	40.0
26	34.65973186662083	36.0	33.0	39.0	27.0	40.0
27	35.08757992437263	37.0	34.0	39.0	27.0	40.0
28	35.03149192162255	37.0	34.0	40.0	27.0	41.0
29	35.08069783430732	37.0	34.0	40.0	27.0	41.0
30	34.448384324510144	36.0	33.0	40.0	24.0	41.0
31	34.567968374011684	37.0	33.0	39.0	25.0	40.0
32	34.01708147129598	37.0	33.0	39.0	23.0	40.0
33	33.89026469577174	37.0	33.0	40.0	21.0	41.0
34	33.490360948779646	37.0	33.0	40.0	16.0	41.0
35	33.3558714334823	37.0	33.0	40.0	15.0	41.0
36	33.32210725335167	37.0	33.0	40.0	15.0	41.0
37	33.059659676864904	37.0	33.0	40.0	12.0	41.0
38	32.89175317978687	37.0	32.0	40.0	13.0	41.0
39	32.626338948092126	36.0	31.0	40.0	12.0	41.0
40	32.496493640426266	36.0	31.0	40.0	12.0	41.0
41	32.023485733929185	35.0	30.0	39.0	12.0	40.0
42	32.41066689584049	36.0	31.0	40.0	10.0	41.0
43	32.69944998281196	37.0	32.0	40.0	11.0	41.0
44	32.99875558611207	37.0	33.0	40.0	12.0	41.0
45	32.94348229632176	37.0	33.0	40.0	12.0	41.0
46	32.795751117222416	36.0	32.0	40.0	12.0	41.0
47	32.46599174974218	35.0	31.0	40.0	12.0	41.0
48	32.5966620831901	36.0	32.0	40.0	11.0	41.0
49	32.75582330697834	36.0	32.0	40.0	12.0	41.0
50	32.614530766586455	36.0	32.0	40.0	11.0	41.0
51	31.66971124097628	35.0	30.0	39.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	10.0
10	17.0
11	16.0
12	16.0
13	22.0
14	24.0
15	46.0
16	84.0
17	177.0
18	415.0
19	763.0
20	1457.0
21	2152.0
22	3113.0
23	4125.0
24	5327.0
25	7000.0
26	8671.0
27	8784.0
28	8804.0
29	9094.0
30	10753.0
31	13108.0
32	16984.0
33	22168.0
34	26025.0
35	33901.0
36	40380.0
37	41049.0
38	23462.0
39	2945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.60948779649364	13.765898934341697	16.810244070127194	21.81436919903747
2	36.27397731179099	32.927466483327606	17.317978686833964	13.48057751804744
3	22.454451701615675	32.8473702303197	32.560330010312825	12.137848057751805
4	20.55379855620488	16.939154348573393	48.355792368511516	14.15125472671021
5	31.763836369886562	19.85733929185287	31.702303196974906	16.676521141285665
6	18.106909590924715	36.62289446545204	33.153661051907875	12.116534891715366
7	57.30457201787556	3.719834994843589	35.37023031969749	3.6053626675833614
8	54.83190099690616	16.014437951185975	23.015125472671023	6.1385355792368514
9	50.57579924372637	4.322103815744242	22.865589549673427	22.236507390855962
10	37.081127535235474	16.41285665177037	30.534891715366104	15.971124097628051
11	30.559642488827777	17.29391543485734	34.18803712616019	17.95840495015469
12	21.52354761086284	15.228257133035408	38.771742866964594	24.476452389137158
13	21.69095909247164	18.825369542798214	45.28979030594706	14.193881058783086
14	17.953592299759364	26.4637332416638	35.71227225850808	19.870402200068753
15	13.0904090752836	18.997937435544863	47.896871777243035	20.0147817119285
16	15.80474389824682	20.294259195599864	35.39841870058439	28.502578205568923
17	16.04159504984531	23.295290477827432	40.14850464077002	20.514609831557237
18	17.104847026469578	19.565830182193196	38.795806118941215	24.533516672396015
19	16.520797524922653	22.059470608456515	35.10244070127192	26.317291165348916
20	21.292196631144723	20.895152973530422	42.47988999656239	15.33276039876246
21	19.170161567548984	27.99759367480234	36.313853557923686	16.51839119972499
22	17.846682708834653	17.254726710209695	40.71158473702303	24.187005843932624
23	19.435201100034377	25.301821931935375	37.2193193537298	18.043657614300447
24	20.693709178411826	20.986249570299073	34.7363355104847	23.5837057408044
25	16.323822619456855	30.18047438982468	33.57958061189412	19.916122378824337
26	16.54039188724648	18.83361980061877	39.09144035751117	25.534547954623584
27	23.645238913716053	21.3970436576143	32.95737366792712	22.000343760742524
28	16.252664145754554	22.442763836369885	39.3881058783087	21.91646613956686
29	20.750773461670676	18.004812650395323	35.352698521828806	25.89171536610519
30	22.81024407012719	24.71467858370574	35.15228600893778	17.322791337229287
31	25.867652114128564	18.794431075971126	31.869714678583705	23.468202131316605
32	25.903059470608458	26.19216225507047	31.096253007906498	16.808525266414577
33	25.384324510140942	20.284977655551735	29.59367480233757	24.73702303196975
34	22.586455826744587	20.061533172911652	34.96115503609488	22.390855964248882
35	23.7273977311791	21.53317291165349	33.37744929529048	21.361980061876935
36	24.558267445857684	26.829494671708492	27.789962186318323	20.822275696115504
37	22.820556892402887	25.639051220350634	33.910965967686494	17.629425919559985
38	26.934685458920594	24.609144035751118	26.233757304915777	22.222413200412515
39	22.11687865245789	26.759023719491232	31.71467858370574	19.409419044345135
40	25.084565142660708	20.023375730491576	34.59951873496047	20.292540391887247
41	19.616019250601582	26.993812306634585	30.826400825025786	22.563767617738055
42	20.4637332416638	22.007562736335508	37.15297353042283	20.375730491577862
43	23.865589549673427	24.65211412856652	27.409762805087656	24.072533516672394
44	23.48710897215538	20.152629769680303	32.38123066345823	23.979030594706085
45	20.97078033688553	18.74527328979031	31.58989343416982	28.694052939154346
46	27.267445857683054	24.982468202131315	28.635613612925404	19.114472327260227
47	18.67033344792025	21.604331385355792	40.24338260570643	19.481952561017533
48	22.236163630113438	22.556204881402543	30.34238569955311	24.865245788930903
49	20.2636644895153	18.687177724303886	39.33447920247507	21.71467858370574
50	22.149192162255073	19.895840495015467	33.5259539360605	24.429013406668957
51	20.937091784118252	18.874183568236507	30.39154348573393	29.79718116191131
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	165.5
2	302.0
3	6044.0
4	11786.0
5	7949.5
6	4113.0
7	4006.5
8	3900.0
9	3814.5
10	3729.0
11	3556.5
12	3384.0
13	3171.5
14	2959.0
15	2758.0
16	2557.0
17	2335.5
18	2114.0
19	2003.0
20	1892.0
21	1816.5
22	1741.0
23	1659.5
24	1578.0
25	1494.0
26	1624.0
27	1838.0
28	2040.5
29	2243.0
30	2404.5
31	2566.0
32	3055.0
33	3544.0
34	3863.0
35	4182.0
36	4754.0
37	5326.0
38	5924.5
39	6523.0
40	6828.0
41	7133.0
42	8342.0
43	9551.0
44	11152.5
45	12754.0
46	33918.0
47	55082.0
48	39519.5
49	23957.0
50	24215.5
51	24474.0
52	21087.0
53	17700.0
54	16262.0
55	14824.0
56	13822.5
57	12821.0
58	11501.5
59	10182.0
60	9286.0
61	8390.0
62	7816.5
63	7243.0
64	6588.5
65	5934.0
66	5047.5
67	4161.0
68	3686.0
69	3211.0
70	2686.5
71	2162.0
72	1828.0
73	1494.0
74	1234.0
75	748.0
76	522.0
77	448.0
78	374.0
79	245.0
80	116.0
81	110.0
82	104.0
83	58.0
84	12.0
85	7.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	290900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.27122697453561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.83849340868278	27.45459168373544
2	7.872655522679761	5.553564396655382
3	2.80089437780385	2.9637294399366088
4	1.55335800730143	2.1915537139296433
5	1.1814788628959407	2.0836104569409484
6	0.9465805268735263	2.003223396781899
7	0.8866069689481842	2.1890200947283516
8	0.8286326595077417	2.338151248960851
9	0.6996898204731369	2.221102662271207
>10	5.29465282686025	29.29387622157393
>50	0.06197252695206724	1.4440692863583062
>100	0.02099069461279697	1.5480732999021782
>500	0.0029986706589709955	0.5841852557362581
>1k	0.006996898204265656	5.056357838122761
>5k	9.995568863236653E-4	2.400553654983815
>10k+	0.0029986706589709955	10.67433734938243
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	10170	3.496046751460983	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	10081	3.465452045376418	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC	10026	3.446545204537642	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6809	2.3406668958404953	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3617	1.2433826057064283	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAGTTAT	2160	0.7425232038501204	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	1913	0.657614300446889	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	1913	0.657614300446889	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA	1906	0.6552079752492266	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	1431	0.49192162255070465	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC	1402	0.4819525610175318	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATG	562	0.19319353729804056	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	561	0.19284977655551735	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	534	0.18356823650739085	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGT	450	0.15469233413544173	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTAT	421	0.14472327260226883	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGGAGTTA	366	0.1258164317634926	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	298	0.10244070127191475	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19903746992093502	0.0
2	0.0	0.0	0.0	1.861808181505672	0.0
3	0.0	0.0	0.0	2.118253695427982	0.0
4	0.0	0.0	0.0	3.1354417325541424	0.0
5	0.0	0.0	0.0	7.529047782743211	0.0
6	0.0	0.0	0.0	8.442763836369886	0.0
7	0.0	0.0	0.0	9.524922653832933	0.0
8	0.0	0.0	0.0	10.59333104159505	0.0
9	0.0	0.0	0.0	10.826744585768305	0.0
10	0.0	0.0	0.0	14.958061189412168	0.0
11	0.0	0.0	0.0	15.772774149192163	0.0
12	0.0	0.0	0.0	20.025438294946717	0.0
13	0.0	0.0	0.0	20.490546579580613	0.0
14	0.0	0.0	0.0	20.747335854245446	0.0
15	0.0	0.0	0.0	21.679614987968375	0.0
16	0.0	0.0	0.0	22.20075627363355	0.0
17	0.0	0.0	0.0	22.782055689240288	0.0
18	0.0	0.0	0.0	23.244070127191474	0.0
19	0.0	0.0	0.0	24.403918872464764	0.0
20	0.0	0.0	0.0	24.838088690271572	0.0
21	0.0	0.0	0.0	25.222756961155035	0.0
22	0.0	0.0	0.0	25.74699209350292	0.0
23	0.0	0.0	0.0	26.131316603643864	0.0
24	0.0	0.0	0.0	26.443795118597457	0.0
25	0.0	0.0	0.0	26.725678927466483	0.0
26	0.0	0.0	0.0	27.090409075283603	0.0
27	0.0	0.0	0.0	27.480233757304916	0.0
28	0.0	0.0	0.0	27.745273289790305	0.0
29	0.0	0.0	0.0	28.03643863870746	0.0
30	0.0	0.0	0.0	28.356823650739084	0.0
31	0.0	0.0	0.0	28.640082502578206	0.0
32	0.0	0.0	0.0	28.914403575111724	0.0
33	0.0	0.0	0.0	29.216225507047096	0.0
34	0.0	0.0	0.0	29.507047095221726	0.0
35	0.0	0.0	0.0	29.810587830869714	0.0
36	0.0	0.0	0.0	30.061876933654176	0.0
37	0.0	0.0	0.0	30.343073221038157	0.0
38	0.0	0.0	0.0	30.59573736679271	0.0
39	0.0	0.0	0.0	30.909247163973873	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGA	30	2.1598935E-6	45.000004	17
AACAAGG	30	2.1598935E-6	45.000004	2
TACGGGA	30	2.1598935E-6	45.000004	4
AACGGGA	45	3.8198777E-10	45.000004	4
ACGGCTA	65	0.0	45.000004	30
CGGGCTT	30	2.1598935E-6	45.000004	6
GACGGGC	30	2.1598935E-6	45.000004	4
GCGATAC	30	2.1598935E-6	45.000004	33
CGACTGA	30	2.1598935E-6	45.000004	41
CGTGGGA	20	7.0245925E-4	45.0	4
CGAACCC	25	3.8835995E-5	45.0	34
GCAAGCC	20	7.0245925E-4	45.0	40
GCGACGA	25	3.8835995E-5	45.0	33
GATCGCG	20	7.0245925E-4	45.0	10
GCGCGAT	20	7.0245925E-4	45.0	31
GACCGTT	25	3.8835995E-5	45.0	22
CGCGATT	20	7.0245925E-4	45.0	32
CGACAGT	20	7.0245925E-4	45.0	30
ATCACGG	20	7.0245925E-4	45.0	2
AGACGGT	20	7.0245925E-4	45.0	23
>>END_MODULE