SRR3552312_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	844170
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	22901	2.7128422000307997	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	21585	2.5569494296172572	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20924	2.478647665754528	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	15423	1.8270016702796832	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	6684	0.7917836454742527	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	5063	0.5997607117049883	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	4913	0.5819917789070922	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	4081	0.48343343165476144	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	3852	0.4563061942499734	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC	3244	0.38428278664250093	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	1790	0.21204259805489414	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	1283	0.15198360519800513	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	1163	0.1377684589596882	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	1117	0.1323193195683334	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	1061	0.12568558465711882	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	1048	0.12414561048130116	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTG	1038	0.1229610149614414	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGT	971	0.11502422497838113	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	897	0.10625821813141903	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	35	1.2114106E-7	45.000004	28
CACGACG	35	1.2114106E-7	45.000004	26
ACGTAAG	35	1.2114106E-7	45.000004	1
TACGGTA	20	7.032222E-4	45.0	4
ATACCGG	20	7.032222E-4	45.0	2
TACGTTC	20	7.032222E-4	45.0	31
CGTTTTT	12065	0.0	44.0862	1
TACGGCT	1840	0.0	43.04348	7
ACGGCTG	1895	0.0	42.031662	8
TGATACC	2805	0.0	41.149734	4
AATGATA	6255	0.0	41.11511	2
ATGATAC	6280	0.0	40.98726	3
GAATGAT	6520	0.0	40.824387	1
ATACGGC	3460	0.0	40.708096	6
ACAACGG	50	1.0804797E-9	40.5	2
TGATACG	3515	0.0	40.391182	4
GATACCT	2875	0.0	40.147827	5
GGCGACT	545	0.0	40.04587	10
CGGTCTA	45	1.9272193E-8	40.0	31
GAATCTG	2720	0.0	39.705883	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content