##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	844170
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.82685833422178	30.0	25.0	31.0	16.0	33.0
2	28.481297605932454	31.0	27.0	33.0	16.0	33.0
3	28.32679436606371	31.0	27.0	33.0	16.0	34.0
4	32.42989682173022	35.0	32.0	35.0	28.0	37.0
5	33.630178755463945	35.0	33.0	35.0	30.0	37.0
6	33.370068824999706	35.0	33.0	35.0	28.0	37.0
7	34.28412049705628	35.0	33.0	36.0	32.0	37.0
8	34.25297274245709	35.0	35.0	37.0	31.0	37.0
9	36.09012758093749	39.0	35.0	39.0	30.0	39.0
10	35.99928687349704	37.0	35.0	39.0	31.0	39.0
11	36.16231327813118	37.0	35.0	39.0	32.0	39.0
12	36.07588755819325	37.0	35.0	39.0	32.0	39.0
13	36.14036864612578	37.0	35.0	39.0	32.0	39.0
14	37.2103012426407	39.0	36.0	40.0	32.0	41.0
15	37.198252721608206	39.0	36.0	40.0	32.0	41.0
16	37.07424807799377	39.0	36.0	40.0	32.0	41.0
17	36.16888422947984	38.0	34.0	40.0	29.0	41.0
18	35.86464811590083	38.0	34.0	39.0	29.0	41.0
19	35.390745939798855	37.0	34.0	39.0	28.0	40.0
20	35.64550742149093	37.0	34.0	39.0	30.0	40.0
21	36.21190636957011	38.0	35.0	39.0	31.0	40.0
22	36.67828754871649	38.0	35.0	40.0	32.0	41.0
23	36.918538919885805	38.0	35.0	40.0	32.0	41.0
24	36.84105689612282	38.0	35.0	40.0	32.0	41.0
25	36.11440586611702	38.0	35.0	40.0	31.0	41.0
26	36.33654477178767	38.0	35.0	40.0	31.0	41.0
27	36.715806058021485	38.0	35.0	40.0	32.0	41.0
28	36.62670907518628	38.0	35.0	40.0	32.0	41.0
29	36.6922574836822	38.0	35.0	40.0	32.0	41.0
30	36.35450442446427	38.0	35.0	40.0	31.0	41.0
31	36.22715092931519	38.0	35.0	40.0	31.0	41.0
32	35.95051707594442	38.0	35.0	40.0	30.0	41.0
33	35.50752218155111	38.0	35.0	40.0	25.0	41.0
34	35.03878958029781	38.0	35.0	40.0	22.0	41.0
35	34.682285558596014	38.0	35.0	40.0	19.0	41.0
36	34.477326841749885	38.0	35.0	40.0	18.0	41.0
37	34.28489877631283	38.0	34.0	40.0	15.0	41.0
38	34.13539571413341	38.0	34.0	40.0	15.0	41.0
39	33.97471243943755	38.0	34.0	40.0	15.0	41.0
40	33.694284326616675	38.0	33.0	40.0	15.0	41.0
41	33.4366478315979	38.0	33.0	40.0	12.0	41.0
42	33.53223521328643	38.0	33.0	40.0	12.0	41.0
43	33.591732707866896	38.0	33.0	40.0	13.0	41.0
44	33.53688711989291	38.0	33.0	40.0	12.0	41.0
45	33.48749540969236	38.0	33.0	40.0	12.0	41.0
46	33.34480969472973	38.0	33.0	40.0	12.0	41.0
47	33.29389577928616	37.0	33.0	40.0	12.0	41.0
48	33.22419891727969	37.0	33.0	40.0	10.0	41.0
49	33.22091521849865	37.0	33.0	40.0	10.0	41.0
50	33.162617719179785	37.0	33.0	40.0	10.0	41.0
51	31.252171955885665	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	27.0
10	30.0
11	30.0
12	31.0
13	28.0
14	35.0
15	85.0
16	179.0
17	387.0
18	794.0
19	1432.0
20	2221.0
21	3278.0
22	4762.0
23	6978.0
24	10627.0
25	17592.0
26	24055.0
27	24937.0
28	22058.0
29	20160.0
30	21260.0
31	25236.0
32	32621.0
33	45865.0
34	60330.0
35	77795.0
36	107075.0
37	152438.0
38	145891.0
39	35918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39205373325278	16.58777260504401	18.680005212220287	22.340168449482924
2	37.440089081583096	28.962057405498893	19.472499615006456	14.125353897911557
3	23.175189831432057	27.805892178115784	35.736759183576766	13.282158806875394
4	21.42388381487141	19.670327066823035	44.21408010234905	14.691709015956503
5	26.73537320681853	22.926424772261512	33.969579587050006	16.368622433869955
6	19.818875345013446	32.988142198846205	34.798441072295866	12.394541383844485
7	62.23758247746307	4.6325976995155	28.691021950554983	4.438797872466446
8	60.6274802468697	10.690737647630217	23.235959581600863	5.445822523899214
9	56.43981662461353	6.609806081713399	23.20444934077259	13.745927952900484
10	31.62526505324757	24.723100797232785	29.683239157989505	13.968394991530142
11	24.854353980833245	20.25397727945793	37.11479915182961	17.77686958787922
12	22.207849129914592	18.57291777722497	40.47525972256773	18.743973370292714
13	19.83143205752396	20.530106495137236	44.98335643294597	14.655105014392836
14	17.626662875961003	25.81494248788751	37.12534205195636	19.433052584195128
15	15.72965161045761	21.352926543231813	46.22374640179111	16.693675444519467
16	17.756376085385643	23.813568357084474	36.211781987040524	22.218273570489355
17	18.22642358766599	22.20263690962721	39.22491915135577	20.346020351351033
18	18.241704869872184	22.765438241112573	39.70420649869102	19.288650390324225
19	18.194794887285738	24.669793998839097	35.91587002617956	21.219541087695607
20	18.831633438762335	24.86691069334376	40.41152848359928	15.889927384294634
21	18.735681201653694	27.177108876174234	37.25185685347738	16.835353068694694
22	17.46401791108426	21.17239418600519	37.53307982989209	23.830508073018468
23	17.34022767925892	26.63776253598209	37.80304914886812	18.218960635890873
24	20.244145136643095	22.22490730540057	35.209969555895135	22.320978002061196
25	16.672945022921922	29.20880865228568	34.287288105476385	19.830958219316013
26	17.260149022116398	22.427946977504533	37.12842200030799	23.183482000071077
27	19.856190103889027	23.64772498430411	35.22217088974969	21.27391402205717
28	16.31910634113982	22.15726690121658	39.073527843917695	22.450098913725906
29	19.78985275477688	20.228034637573	34.593979885568075	25.388132722082048
30	18.684388215643768	23.72626366727081	38.429818638425914	19.15952947865951
31	22.811755925939085	21.504791688877834	33.08918819669024	22.594264188492836
32	20.659819704561876	24.74300200196643	32.062617719179784	22.53456057429191
33	18.160441617209806	22.597344136844473	32.46040489474869	26.781809351197033
34	19.306774701778078	23.148891810891172	34.33123659926318	23.213096888067568
35	16.446568819076727	26.88249997038511	32.67943660637076	23.991494604167407
36	21.20923510667283	27.131738867763598	29.855123967921156	21.803902057642418
37	18.214340713363423	25.29857730078065	34.86584455737589	21.621237428480043
38	20.34815262328678	26.593695582643306	27.519220062309724	25.53893173176019
39	20.629257140149495	23.992205361479325	32.35118518781762	23.02735231055356
40	18.737813473589444	26.161318217894504	32.92938626106116	22.171482047454898
41	18.177025954487842	29.854768589265195	30.44469715815535	21.523508298091617
42	18.850350047976118	26.51326154684483	34.006775886373596	20.629612518805455
43	21.551464752360307	25.186040726393973	29.48718859945272	23.775305921793002
44	21.272610966985322	22.847412251086865	31.498276413518607	24.381700368409206
45	19.49500692988379	22.19612163426798	31.20094293803381	27.10792849781442
46	23.031616854425057	25.624222609190088	29.715933994337636	21.62822654204722
47	16.916853240461045	23.09534809339351	38.29880237392942	21.68899629221602
48	20.24367129843515	24.761007853868296	30.31948541170617	24.675835435990383
49	18.92118886006373	21.80141440705071	35.82228698011064	23.455109752774916
50	19.29871945224303	21.544949477001076	33.481526232867786	25.674804837888104
51	19.435421775234847	21.723941860051884	30.02795645426869	28.812679910444576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	358.0
1	953.5
2	1549.0
3	16566.5
4	31584.0
5	21488.5
6	11393.0
7	11018.0
8	10643.0
9	10736.0
10	10829.0
11	10765.0
12	10701.0
13	10487.0
14	10273.0
15	9936.5
16	9600.0
17	9102.0
18	8604.0
19	8154.5
20	7705.0
21	7266.0
22	6827.0
23	6409.5
24	5992.0
25	6217.5
26	6811.5
27	7180.0
28	8176.0
29	9172.0
30	9871.5
31	10571.0
32	11690.5
33	12810.0
34	14510.5
35	16211.0
36	17772.5
37	19334.0
38	21056.0
39	22778.0
40	25539.5
41	28301.0
42	32375.0
43	36449.0
44	41625.0
45	46801.0
46	92821.0
47	138841.0
48	106543.5
49	74246.0
50	72187.0
51	70128.0
52	59764.0
53	49400.0
54	44275.0
55	39150.0
56	35035.5
57	30921.0
58	28673.5
59	26426.0
60	24051.0
61	21676.0
62	19356.5
63	17037.0
64	14975.5
65	12914.0
66	10555.5
67	8197.0
68	6789.0
69	5381.0
70	4376.0
71	3371.0
72	2754.0
73	2137.0
74	1627.0
75	828.5
76	540.0
77	406.5
78	273.0
79	210.5
80	148.0
81	116.5
82	85.0
83	55.0
84	25.0
85	25.5
86	26.0
87	16.5
88	7.0
89	7.5
90	8.0
91	5.5
92	3.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	844170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.391285056099356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.55484549288579	33.30040847826763
2	8.461636020000228	7.173992491295754
3	3.270392191363324	4.159083828879724
4	1.8789922273174275	3.1861158050563243
5	1.3109222629180555	2.7785839666873056
6	1.0370453141731903	2.6377010117524695
7	0.8374203210826128	2.48495264799483
8	0.7032797730246358	2.385034666598095
9	0.6031499781010974	2.301147238793733
>10	3.2557394333168834	21.08799515137037
>50	0.051607820379877205	1.5090492539087528
>100	0.026790892875089404	1.9587842814095036
>500	0.00338411278418755	1.0297813732045975
>1k	0.0031021033855052537	2.939670764697127
>5k	5.640187973645916E-4	1.4043234027205023
>10k+	0.0011280375947291831	9.663375637363272
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	22901	2.7128422000307997	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	21585	2.5569494296172572	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20924	2.478647665754528	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	15423	1.8270016702796832	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	6684	0.7917836454742527	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	5063	0.5997607117049883	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	4913	0.5819917789070922	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	4081	0.48343343165476144	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	3852	0.4563061942499734	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC	3244	0.38428278664250093	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	1790	0.21204259805489414	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	1283	0.15198360519800513	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	1163	0.1377684589596882	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	1117	0.1323193195683334	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	1061	0.12568558465711882	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	1048	0.12414561048130116	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTG	1038	0.1229610149614414	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGT	971	0.11502422497838113	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	897	0.10625821813141903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2353791297961311	0.0
2	0.0	0.0	0.0	1.4422450454292381	0.0
3	0.0	0.0	0.0	1.8557873414122747	0.0
4	0.0	0.0	0.0	2.881291682954855	0.0
5	0.0	0.0	0.0	6.564080694646813	0.0
6	0.0	0.0	0.0	7.385123849461602	0.0
7	0.0	0.0	0.0	8.69137732921094	0.0
8	0.0	0.0	0.0	10.179940059466695	0.0
9	1.1845955198597438E-4	0.0	0.0	10.655199782034424	0.0
10	1.1845955198597438E-4	0.0	0.0	14.450880746769016	0.0
11	1.1845955198597438E-4	0.0	0.0	15.869907720009003	0.0
12	1.1845955198597438E-4	0.0	0.0	18.853193077223782	0.0
13	1.1845955198597438E-4	0.0	0.0	19.396093203975504	0.0
14	1.1845955198597438E-4	0.0	0.0	19.67245933875878	0.0
15	1.1845955198597438E-4	0.0	0.0	20.44872478292287	0.0
16	1.1845955198597438E-4	0.0	0.0	21.063056019522133	0.0
17	1.1845955198597438E-4	0.0	0.0	21.74550149851333	0.0
18	1.1845955198597438E-4	0.0	0.0	22.440503690015046	0.0
19	1.1845955198597438E-4	0.0	0.0	23.049859625430898	0.0
20	1.1845955198597438E-4	0.0	0.0	23.53151616380587	0.0
21	1.1845955198597438E-4	0.0	0.0	24.081997701884692	0.0
22	1.1845955198597438E-4	0.0	0.0	24.72440385230463	0.0
23	2.3691910397194877E-4	0.0	0.0	25.280097610670836	0.0
24	2.3691910397194877E-4	0.0	0.0	25.759977255766017	0.0
25	2.3691910397194877E-4	0.0	0.0	26.159422865062723	0.0
26	2.3691910397194877E-4	0.0	0.0	26.548325574232678	0.0
27	3.553786559579232E-4	0.0	0.0	26.973003068102397	0.0
28	3.553786559579232E-4	0.0	0.0	27.404314296883328	0.0
29	3.553786559579232E-4	0.0	0.0	27.831716360448723	0.0
30	3.553786559579232E-4	0.0	0.0	28.313372898823697	0.0
31	4.7383820794389753E-4	0.0	0.0	28.727981330774607	0.0
32	4.7383820794389753E-4	0.0	0.0	29.160003316867456	0.0
33	4.7383820794389753E-4	0.0	0.0	29.561107359891963	0.0
34	4.7383820794389753E-4	0.0	0.0	29.972872762595212	0.0
35	4.7383820794389753E-4	0.0	0.0	30.40548704644799	0.0
36	4.7383820794389753E-4	0.0	0.0	30.809315659168178	0.0
37	4.7383820794389753E-4	0.0	0.0	31.209945863984743	0.0
38	4.7383820794389753E-4	0.0	0.0	31.644100122013338	0.0
39	4.7383820794389753E-4	0.0	0.0	32.13203501664357	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	35	1.2114106E-7	45.000004	28
CACGACG	35	1.2114106E-7	45.000004	26
ACGTAAG	35	1.2114106E-7	45.000004	1
TACGGTA	20	7.032222E-4	45.0	4
ATACCGG	20	7.032222E-4	45.0	2
TACGTTC	20	7.032222E-4	45.0	31
CGTTTTT	12065	0.0	44.0862	1
TACGGCT	1840	0.0	43.04348	7
ACGGCTG	1895	0.0	42.031662	8
TGATACC	2805	0.0	41.149734	4
AATGATA	6255	0.0	41.11511	2
ATGATAC	6280	0.0	40.98726	3
GAATGAT	6520	0.0	40.824387	1
ATACGGC	3460	0.0	40.708096	6
ACAACGG	50	1.0804797E-9	40.5	2
TGATACG	3515	0.0	40.391182	4
GATACCT	2875	0.0	40.147827	5
GGCGACT	545	0.0	40.04587	10
CGGTCTA	45	1.9272193E-8	40.0	31
GAATCTG	2720	0.0	39.705883	1
>>END_MODULE