SRR3552306_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	630192
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG	15680	2.488130601467489	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	13480	2.1390306446289387	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	12454	1.9762231193033233	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8996	1.4275014598725468	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	5289	0.8392680325995886	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT	3407	0.5406288877040648	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT	3101	0.4920722573437937	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTA	2653	0.42098281158757966	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTC	2140	0.33957904892477214	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	1863	0.29562419072282736	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	1813	0.28769010079467844	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC	1614	0.2561124228806459	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGGGTTT	958	0.15201716302333257	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	896	0.14217889151242796	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGGTTCGTATGC	869	0.13789448295122758	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTT	844	0.13392743798715312	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTAT	719	0.11409221316678092	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGGTTCGTATGCCGTCTTC	677	0.10742757762713585	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGT	668	0.10599944144006905	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.2107557E-7	45.000004	6
GCGATAC	30	2.1639353E-6	45.000004	9
AACACGT	35	1.2107557E-7	45.000004	41
ACACGAC	40	6.8066583E-9	45.0	26
CGACGTC	25	3.8887934E-5	45.0	18
GTATGCG	20	7.0308585E-4	45.0	1
ACGCTAG	20	7.0308585E-4	45.0	1
ACGTCGC	25	3.8887934E-5	45.0	20
TGCGTAG	25	3.8887934E-5	45.0	1
CGTTTTT	4465	0.0	42.83315	1
TACGGCT	1725	0.0	42.130436	7
ATACGGC	2800	0.0	41.142853	6
ACGGCTG	1770	0.0	41.059322	8
ATGATAC	4835	0.0	40.904858	3
TACGGGA	160	0.0	40.78125	4
TGATACG	2845	0.0	40.72935	4
TGATACC	2035	0.0	40.68796	4
GAATGAT	4910	0.0	40.55499	1
CGATAAG	50	1.0804797E-9	40.5	10
AATGATA	4875	0.0	40.476925	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content