##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	630192
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.706383134028993	30.0	25.0	31.0	16.0	33.0
2	28.28960380328535	31.0	27.0	33.0	16.0	34.0
3	28.22525198669612	31.0	26.0	33.0	16.0	34.0
4	32.71973462056008	35.0	32.0	35.0	28.0	37.0
5	33.67316785995379	35.0	33.0	35.0	30.0	37.0
6	33.3060511717064	35.0	33.0	35.0	28.0	37.0
7	34.32568804427857	35.0	33.0	36.0	32.0	37.0
8	34.20399179932465	35.0	35.0	37.0	30.0	37.0
9	35.94338233427273	39.0	35.0	39.0	30.0	39.0
10	35.8696333815726	37.0	35.0	39.0	31.0	39.0
11	36.03516864701551	37.0	35.0	39.0	31.0	39.0
12	35.90329613831975	37.0	35.0	39.0	31.0	39.0
13	36.078265036687235	37.0	35.0	39.0	32.0	39.0
14	37.11282275877828	39.0	36.0	40.0	32.0	41.0
15	37.11346700688044	39.0	36.0	40.0	32.0	41.0
16	36.893995798105976	38.0	35.0	40.0	32.0	41.0
17	35.91571140223932	38.0	34.0	40.0	29.0	41.0
18	35.721802244395356	38.0	33.0	39.0	29.0	41.0
19	35.3374447787341	37.0	33.0	39.0	27.0	41.0
20	35.897558204483715	37.0	34.0	39.0	30.0	41.0
21	36.500514129027344	38.0	35.0	40.0	31.0	41.0
22	36.98582019448041	39.0	35.0	40.0	32.0	41.0
23	37.224794983116254	39.0	35.0	40.0	33.0	41.0
24	37.17749828623658	39.0	35.0	40.0	33.0	41.0
25	36.44838081092746	38.0	35.0	40.0	31.0	41.0
26	36.67363438444157	38.0	35.0	40.0	31.0	41.0
27	37.0004189199482	39.0	35.0	40.0	32.0	41.0
28	36.97698161830046	39.0	35.0	40.0	32.0	41.0
29	36.96020577855638	39.0	35.0	40.0	32.0	41.0
30	36.626832774773405	39.0	35.0	40.0	31.0	41.0
31	36.606586564094755	39.0	35.0	40.0	31.0	41.0
32	36.40853422449032	39.0	35.0	40.0	30.0	41.0
33	36.373384619290626	39.0	35.0	40.0	30.0	41.0
34	36.17389938304517	39.0	35.0	40.0	30.0	41.0
35	35.61994598471577	39.0	35.0	40.0	26.0	41.0
36	35.64498279889303	39.0	35.0	40.0	27.0	41.0
37	35.389858963617435	38.0	35.0	40.0	25.0	41.0
38	35.34305735394927	38.0	35.0	40.0	25.0	41.0
39	35.30586551400208	38.0	35.0	40.0	25.0	41.0
40	34.84548518546729	38.0	34.0	40.0	23.0	41.0
41	34.72349379236804	38.0	34.0	40.0	22.0	41.0
42	34.552014306750955	38.0	34.0	40.0	22.0	41.0
43	34.70091495925051	38.0	34.0	40.0	23.0	41.0
44	34.74456990885317	38.0	34.0	40.0	23.0	41.0
45	34.59453626831188	38.0	34.0	40.0	23.0	41.0
46	34.42622089775814	38.0	34.0	40.0	22.0	41.0
47	34.41132067687308	38.0	34.0	40.0	22.0	41.0
48	34.270842854241245	37.0	34.0	40.0	22.0	41.0
49	34.25083783989641	37.0	34.0	40.0	21.0	41.0
50	34.167444207479626	37.0	34.0	40.0	21.0	41.0
51	31.873524259273363	35.0	30.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	24.0
10	18.0
11	23.0
12	19.0
13	23.0
14	33.0
15	51.0
16	118.0
17	223.0
18	456.0
19	763.0
20	1099.0
21	1828.0
22	2672.0
23	3868.0
24	5532.0
25	8113.0
26	11357.0
27	13058.0
28	13532.0
29	14004.0
30	15448.0
31	19271.0
32	25531.0
33	36479.0
34	48797.0
35	61288.0
36	85742.0
37	119270.0
38	112901.0
39	28645.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7092314723132	17.496413791352477	21.641182369817454	16.15317236651687
2	32.779375174549976	30.882492954528146	21.10277502729327	15.235356843628608
3	27.34976007312057	28.9616497829233	29.664451468758728	14.0241386751974
4	24.94890446086272	20.531203188869423	38.34450453195217	16.175387818315688
5	28.76615380709371	24.55235864625384	27.65665067154137	19.024836875111077
6	21.557715743773326	36.02584609134994	28.72442049407165	13.692017670805088
7	66.93674308782084	5.311397161499987	22.74576636961434	5.006093381064818
8	65.4119696854292	11.648037423515373	16.89850077436718	6.041492116688247
9	60.40841521314139	6.80316474979054	17.53386269581334	15.25455734125473
10	36.546322390636504	21.597703557011197	25.401464950364332	16.454509101987966
11	28.60747200853073	21.362854495137988	30.130658592936754	19.899014903394523
12	25.061885901439563	18.828547490288674	35.423489984004874	20.686076624266892
13	21.48853047959987	21.118008479955318	39.83595475664559	17.557506283799224
14	19.037055373600424	26.617443572752432	32.71495036433341	21.630550689313733
15	16.213630131769367	22.49917485464747	42.69873308452027	18.588461929062888
16	18.05354558610709	23.29639221062787	33.55072739736461	25.099334805900426
17	17.872965699342423	22.48203722040267	36.44365526696626	23.201341813288646
18	18.47072003452916	23.740383883007084	35.98649300530632	21.802403077157436
19	19.892350267854873	25.092035443166527	31.977080001015562	23.038534287963035
20	21.059454896285576	25.182960113743114	36.60201970193211	17.155565288039202
21	20.699088531749055	28.141106202554145	32.629420874907964	18.53038439078884
22	18.565294386472694	22.925394165587633	32.685435549800694	25.823875898138983
23	19.056731916622237	27.760904613197248	33.27589052225354	19.906472947926982
24	21.959022012339098	22.219418843780943	30.371854926752484	25.44970421712748
25	18.282999466829157	30.02386574250387	29.51164089674258	22.181493893924394
26	19.150512859572956	23.871613730418666	32.83792875821972	24.13994465178866
27	21.172912382258104	25.06379008302232	31.285703404676667	22.477594130042906
28	16.87882423134537	23.50204382156549	36.801165359128646	22.817966587960495
29	25.927177749003476	21.352540178231397	30.606545306827126	22.113736765938
30	21.297160230532917	24.534427603016223	35.0954947063752	19.072917460075658
31	24.556325691217914	22.84002335796075	31.159075329423413	21.444575621397924
32	27.612537131540865	24.648043770787314	29.4965661258791	18.242852971792722
33	24.166761875745806	21.64943382334273	30.149700408764314	24.034103892147154
34	29.523859395231927	21.35333358722421	30.257445349988576	18.865361667555288
35	23.13961459364765	21.12419707009927	33.69941224261812	22.036776093634955
36	24.907647193236347	22.63595856500876	34.41792342651129	18.03847081524361
37	24.26403381826491	22.798924772132935	35.395879351054916	17.541162058547236
38	24.281806179703963	23.12089014141722	31.44295706705258	21.154346611826234
39	31.043237616472442	20.564050321171962	29.526874349404626	18.865837712950974
40	26.526518902175845	20.354907710665955	34.211161042983726	18.907412344174475
41	22.78051768349963	23.470307461852897	32.82888389570163	20.920290958945845
42	21.631185416507982	21.097062482545002	37.612981440576846	19.658770660370173
43	24.231345367760937	20.745582298728007	32.696067230304415	22.327005103206645
44	23.588525401782316	20.881731281895043	32.02008276842613	23.509660547896512
45	22.7287874171681	19.555151445908546	31.054186660573286	26.661874476350068
46	26.40322314469241	23.38192170005332	30.4154924213573	19.79936273389697
47	19.887907177495112	20.91521314139183	39.2693020539772	19.927577627135857
48	22.440303907380606	22.408567547668014	31.622108817630185	23.5290197273212
49	21.282085459669435	19.565465762815144	37.06362505395181	22.088823723563614
50	22.395873003782974	19.413765963388936	33.150055855993095	25.040305176835
51	21.89650138370528	19.306497067560365	30.025928605885188	28.771072942849163
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	301.0
1	819.5
2	1338.0
3	8136.5
4	14935.0
5	9955.5
6	4976.0
7	4778.5
8	4581.0
9	4614.0
10	4647.0
11	4560.0
12	4473.0
13	4243.5
14	4014.0
15	3820.5
16	3627.0
17	3445.0
18	3263.0
19	3152.0
20	3041.0
21	3053.5
22	3066.0
23	3041.0
24	3016.0
25	3330.5
26	4553.0
27	5461.0
28	5951.0
29	6441.0
30	7425.0
31	8409.0
32	9299.0
33	10189.0
34	11259.0
35	12329.0
36	13294.0
37	14259.0
38	15548.0
39	16837.0
40	18635.5
41	20434.0
42	23740.0
43	27046.0
44	31443.5
45	35841.0
46	67893.0
47	99945.0
48	80641.5
49	61338.0
50	59979.5
51	58621.0
52	49306.0
53	39991.0
54	35567.5
55	31144.0
56	28587.5
57	26031.0
58	24561.0
59	23091.0
60	22679.0
61	22267.0
62	19705.5
63	17144.0
64	14945.5
65	12747.0
66	10515.0
67	8283.0
68	6935.5
69	5588.0
70	4502.5
71	3417.0
72	2791.5
73	2166.0
74	1662.5
75	880.0
76	601.0
77	413.5
78	226.0
79	166.5
80	107.0
81	89.5
82	72.0
83	50.5
84	29.0
85	24.5
86	20.0
87	16.5
88	13.0
89	11.5
90	10.0
91	7.0
92	4.0
93	4.5
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	630192.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.46507654673651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35963231111667	30.135387342388743
2	9.146805730324937	7.219587766108036
3	3.33986660754937	3.9542427396847524
4	1.7904929637055207	2.8264776747612594
5	1.3161447089302925	2.5970875842258114
6	1.0756079018326559	2.546936890806025
7	0.9152911683316004	2.5285425214530957
8	0.8074793734720577	2.5493788227188476
9	0.7646017077447524	2.7157558431520905
>10	4.399319836498785	26.755519481196107
>50	0.04542038918311566	1.2477213553975508
>100	0.030009899968603454	2.0713198781762867
>500	0.0044609310764140275	1.2502820961453525
>1k	0.0028387743213543807	2.6553290139717802
>5k	8.110783775298231E-4	2.2862621279360424
>10k+	0.0012166175662947348	6.660168861878227
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG	15680	2.488130601467489	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	13480	2.1390306446289387	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	12454	1.9762231193033233	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8996	1.4275014598725468	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	5289	0.8392680325995886	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCT	3407	0.5406288877040648	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCT	3101	0.4920722573437937	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTA	2653	0.42098281158757966	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTC	2140	0.33957904892477214	No Hit
CTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGCT	1863	0.29562419072282736	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	1813	0.28769010079467844	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCC	1614	0.2561124228806459	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGGGTTT	958	0.15201716302333257	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTCTGC	896	0.14217889151242796	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGGTTCGTATGC	869	0.13789448295122758	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTT	844	0.13392743798715312	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTAT	719	0.11409221316678092	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGGTTCGTATGCCGTCTTC	677	0.10742757762713585	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGT	668	0.10599944144006905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5868179856297763E-4	0.0	0.0	0.33894432173052025	0.0
2	1.5868179856297763E-4	0.0	0.0	1.8927564932591971	0.0
3	1.5868179856297763E-4	0.0	0.0	2.3451583009622463	0.0
4	1.5868179856297763E-4	0.0	0.0	3.327874679462767	0.0
5	1.5868179856297763E-4	0.0	0.0	6.976603955619875	0.0
6	1.5868179856297763E-4	0.0	0.0	7.801907989945922	0.0
7	1.5868179856297763E-4	0.0	0.0	9.12832914413385	0.0
8	1.5868179856297763E-4	0.0	0.0	10.53091756163201	0.0
9	1.5868179856297763E-4	0.0	0.0	11.00251986696118	0.0
10	1.5868179856297763E-4	0.0	0.0	14.881020387437479	0.0
11	1.5868179856297763E-4	0.0	0.0	16.668570848249423	0.0
12	1.5868179856297763E-4	0.0	0.0	20.394895523903827	0.0
13	1.5868179856297763E-4	0.0	0.0	21.08896971081829	0.0
14	1.5868179856297763E-4	0.0	0.0	21.440291212836723	0.0
15	1.5868179856297763E-4	0.0	0.0	22.330178739177903	0.0
16	1.5868179856297763E-4	0.0	0.0	23.220859674511896	0.0
17	1.5868179856297763E-4	0.0	0.0	24.17517201106964	0.0
18	3.1736359712595527E-4	0.0	0.0	25.136783710361286	0.0
19	3.1736359712595527E-4	0.0	0.0	25.939237565694263	0.0
20	3.1736359712595527E-4	0.0	0.0	26.604907710665955	0.0
21	3.1736359712595527E-4	0.0	0.0	27.287715489882448	0.0
22	3.1736359712595527E-4	0.0	0.0	28.061765303272654	0.0
23	3.1736359712595527E-4	0.0	0.0	28.753141899611546	0.0
24	3.1736359712595527E-4	0.0	0.0	29.310908421560413	0.0
25	3.1736359712595527E-4	0.0	0.0	29.78663645365222	0.0
26	4.7604539568893287E-4	0.0	0.0	30.238244852362456	0.0
27	4.7604539568893287E-4	0.0	0.0	30.701437022367788	0.0
28	4.7604539568893287E-4	0.0	0.0	31.16843755553863	0.0
29	4.7604539568893287E-4	0.0	0.0	31.638611724680732	0.0
30	4.7604539568893287E-4	0.0	0.0	32.14337852590956	0.0
31	4.7604539568893287E-4	0.0	0.0	32.62529514814533	0.0
32	4.7604539568893287E-4	0.0	0.0	33.09356513570467	0.0
33	4.7604539568893287E-4	0.0	0.0	33.57802066671744	0.0
34	6.347271942519105E-4	0.0	0.0	34.044386472694036	0.0
35	6.347271942519105E-4	0.0	0.0	34.52360550435423	0.0
36	6.347271942519105E-4	0.0	0.0	34.98251326579836	0.0
37	7.934089928148881E-4	0.0	0.0	35.43951684565974	0.0
38	7.934089928148881E-4	0.0	0.0	35.905882651636325	0.0
39	7.934089928148881E-4	0.0	0.0	36.40811054408815	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.2107557E-7	45.000004	6
GCGATAC	30	2.1639353E-6	45.000004	9
AACACGT	35	1.2107557E-7	45.000004	41
ACACGAC	40	6.8066583E-9	45.0	26
CGACGTC	25	3.8887934E-5	45.0	18
GTATGCG	20	7.0308585E-4	45.0	1
ACGCTAG	20	7.0308585E-4	45.0	1
ACGTCGC	25	3.8887934E-5	45.0	20
TGCGTAG	25	3.8887934E-5	45.0	1
CGTTTTT	4465	0.0	42.83315	1
TACGGCT	1725	0.0	42.130436	7
ATACGGC	2800	0.0	41.142853	6
ACGGCTG	1770	0.0	41.059322	8
ATGATAC	4835	0.0	40.904858	3
TACGGGA	160	0.0	40.78125	4
TGATACG	2845	0.0	40.72935	4
TGATACC	2035	0.0	40.68796	4
GAATGAT	4910	0.0	40.55499	1
CGATAAG	50	1.0804797E-9	40.5	10
AATGATA	4875	0.0	40.476925	2
>>END_MODULE