FastQCFastQC Report
Sat 18 Jun 2016
SRR3552304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578999
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81231.4029385197556472No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC79921.3803132647897491No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCG79321.369950552591628No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC77831.3442164839662936No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC28130.4858384902219175Illumina Single End Adapter 1 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC19030.328670688550412No Hit
GAACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCT18630.3217622137516645Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCT18160.3136447558631362No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTA15040.25975865243290575No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTC14660.25319560137409564No Hit
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT10940.18894678574574394Illumina Single End Adapter 1 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC9970.17219373435878127Illumina Single End Adapter 1 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT7670.13247000426598318No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC6240.10777220686046089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA302.1636279E-645.00000428
CATGACG253.8883984E-545.01
CTACCGT351.2105374E-745.027
GTACGCG253.8883984E-545.01
CGTTTTT48650.043.4275471
TAACGGG1700.041.029413
TACGGCT9300.040.8870937
CGCACGG1050.040.7142872
TTAACGG900.040.02
TAATCGT403.454261E-739.37521
ACGGCTG9850.038.6040578
TTAATCG356.242919E-638.57142620
TAGCGAG356.242919E-638.5714261
GTTAACG356.242919E-638.5714261
ACTACCG356.242919E-638.57142626
ACCGTAT356.242919E-638.57142629
TACGGGA1950.038.0769234
CGCATCG301.1392219E-437.50000421
TACGCGG601.546141E-1037.5000042
GCGATAC301.1392219E-437.5000049
Produced by FastQC (version 0.11.5)