SRR3552303_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665153
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	12336	1.8546108940349062	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	10957	1.6472901723362896	RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10424	1.5671582327674987	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	8933	1.3429992798649335	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	3640	0.5472425141283284	Illumina PCR Primer Index 6 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	2652	0.3987052602934964	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	2595	0.39013580334148684	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	2264	0.34037281647981743	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	2078	0.31240932537326	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	1969	0.2960221182194172	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	1817	0.2731702330140584	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	1157	0.17394494199079008	RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	956	0.14372633063370382	RNA PCR Primer, Index 27 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	25	3.8890263E-5	45.000004	1
CACTGCG	25	3.8890263E-5	45.000004	1
GGGTCGA	20	7.031141E-4	45.0	7
ACGGATA	20	7.031141E-4	45.0	5
CGTTTTT	6395	0.0	43.6982	1
ACGGCTG	1190	0.0	41.596638	8
TACGGCT	1205	0.0	41.26556	7
AACGGGC	60	3.6379788E-12	41.250004	4
ACCACCG	1045	0.0	40.47847	14
TGATACC	1425	0.0	40.26316	4
CGGCGAT	95	0.0	40.263157	31
CGGCTGT	1265	0.0	40.197628	9
CGAACCC	45	1.9261279E-8	40.0	34
GCGAACC	45	1.9261279E-8	40.0	33
GATACCT	1435	0.0	39.825787	5
CGACCAC	1460	0.0	39.452057	12
TCACGAC	80	0.0	39.375	25
CGACGGT	80	0.0	39.375	28
CCGTAGG	40	3.4552977E-7	39.375	2
CGATGAA	680	0.0	39.375	19