##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552303_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665153
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.115803431691656	28.0	16.0	31.0	16.0	33.0
2	26.059033034504843	28.0	23.0	31.0	16.0	33.0
3	26.459002665552138	30.0	23.0	33.0	16.0	34.0
4	30.186914890258333	33.0	28.0	35.0	19.0	37.0
5	32.04200537319985	33.0	32.0	35.0	28.0	37.0
6	30.35232796063462	35.0	28.0	35.0	17.0	37.0
7	32.83905657796026	35.0	32.0	35.0	28.0	37.0
8	33.356525491127606	35.0	33.0	36.0	28.0	37.0
9	34.9663085034571	37.0	34.0	39.0	28.0	39.0
10	34.86804840390106	37.0	34.0	39.0	30.0	39.0
11	35.076266663459386	37.0	34.0	39.0	30.0	39.0
12	34.920284505970805	37.0	34.0	39.0	27.0	39.0
13	35.22802122218497	37.0	34.0	39.0	30.0	39.0
14	35.44684456057478	38.0	34.0	40.0	27.0	41.0
15	36.03576320034639	38.0	34.0	40.0	30.0	41.0
16	35.76299137190992	38.0	34.0	40.0	27.0	41.0
17	35.35668334954514	37.0	33.0	40.0	27.0	41.0
18	35.31287538355837	37.0	33.0	39.0	27.0	40.0
19	34.85297367673302	37.0	32.0	39.0	27.0	40.0
20	35.309163455626	37.0	34.0	39.0	29.0	40.0
21	35.7786644576511	37.0	34.0	39.0	30.0	40.0
22	36.46314607315911	38.0	35.0	40.0	31.0	41.0
23	36.90524886755378	38.0	35.0	40.0	32.0	41.0
24	36.868673823917206	38.0	35.0	40.0	32.0	41.0
25	35.066363678732564	37.0	34.0	40.0	27.0	41.0
26	35.7842240807754	37.0	34.0	40.0	30.0	41.0
27	36.310851788986895	38.0	35.0	40.0	31.0	41.0
28	36.33573779265823	38.0	35.0	40.0	31.0	41.0
29	36.53237525802334	38.0	35.0	40.0	31.0	41.0
30	35.558047546955365	38.0	34.0	40.0	29.0	41.0
31	35.583337968858295	37.0	34.0	40.0	30.0	41.0
32	35.51355703123943	38.0	35.0	40.0	30.0	41.0
33	35.58499022029518	38.0	35.0	40.0	28.0	41.0
34	35.501639472422134	39.0	35.0	40.0	26.0	41.0
35	35.41332896341142	39.0	35.0	40.0	25.0	41.0
36	35.23047479301755	39.0	35.0	40.0	24.0	41.0
37	35.39923596525912	39.0	35.0	40.0	24.0	41.0
38	35.04143407606971	38.0	35.0	40.0	23.0	41.0
39	34.97674971021705	38.0	35.0	40.0	23.0	41.0
40	34.681291372060265	38.0	34.0	40.0	23.0	41.0
41	34.43049343534495	38.0	34.0	40.0	22.0	41.0
42	34.84852958642598	38.0	34.0	40.0	23.0	41.0
43	34.79693844874788	38.0	34.0	40.0	22.0	41.0
44	34.95570041779861	38.0	35.0	40.0	23.0	41.0
45	34.94896061507653	38.0	35.0	40.0	23.0	41.0
46	34.77389412661448	38.0	34.0	40.0	23.0	41.0
47	34.68465150123355	38.0	34.0	40.0	23.0	41.0
48	34.666365482828766	38.0	34.0	40.0	23.0	41.0
49	34.78030468177998	38.0	34.0	40.0	23.0	41.0
50	34.70688247666327	38.0	34.0	40.0	23.0	41.0
51	33.608202924740624	37.0	33.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	24.0
10	28.0
11	25.0
12	29.0
13	29.0
14	50.0
15	75.0
16	165.0
17	252.0
18	540.0
19	1002.0
20	1914.0
21	2876.0
22	4015.0
23	5300.0
24	7280.0
25	10585.0
26	13367.0
27	14391.0
28	14520.0
29	15449.0
30	19001.0
31	25076.0
32	34430.0
33	45943.0
34	57745.0
35	84599.0
36	115816.0
37	120867.0
38	63154.0
39	6596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48203496037754	17.533559947861622	21.970433870102067	19.013971221658778
2	34.97360757600131	26.16315343988526	21.544516825452188	17.31872215866124
3	26.987775744828635	24.567430350611062	31.71014789078603	16.734646013774274
4	23.764457200072762	21.39643059566746	37.983591745057154	16.85552045920262
5	27.429628972582247	25.250731786521296	29.77540505718233	17.54423418371412
6	22.347640317340524	32.25438357791365	30.444424064839215	14.95355203990661
7	69.42447827793004	4.266086148600397	22.388683505900147	3.920752067569416
8	69.83611289432656	9.049647224022142	16.40870596689784	4.705533914753448
9	64.37240755134533	5.973212178250718	17.300831538007046	12.353548732396908
10	34.871074775277265	27.16277307626967	24.829024299672405	13.137127848780656
11	26.529685651271212	20.88917888064851	35.084409150977294	17.496726317102983
12	22.392592381001066	19.28503667577234	37.79867188451379	20.523699058712808
13	21.674712434582723	20.60879226283276	41.212021895714216	16.5044734068703
14	18.569411849604528	25.636808373411828	35.83536419440339	19.95841558258025
15	16.751484244978222	22.75551639998617	41.99875818044871	18.4942411745869
16	19.43086778530654	23.71995616046233	35.01750725021161	21.83166880401953
17	19.30217558967636	23.54856702142214	36.927443761059486	20.221813627842014
18	20.180920780632423	22.58021838584506	36.724332597161855	20.514528236360658
19	19.339309903135067	25.30695945143448	33.88137766799518	21.472352977435268
20	22.107094157284113	24.186465369621725	37.39425365291895	16.31218682017521
21	20.751917228066326	27.165779903270376	34.58287040725968	17.499432461403615
22	19.68238886391552	21.365009253510095	36.71591348155988	22.236688401014504
23	19.772292991236604	25.770461833593174	35.56805727404071	18.889187901129514
24	20.76063702636837	23.08536532196352	34.23513086462814	21.918866787039974
25	18.422227667920012	27.58042134666761	32.73066497482534	21.266686010587037
26	19.207610880504184	23.191205632388336	35.60759704909998	21.993586438007494
27	20.99351577757298	23.716648650761556	32.93573057627343	22.35410499539204
28	16.79057299598739	24.68980820953976	36.16295799613021	22.356660798342638
29	22.761680395337613	21.54481750815226	33.695255076651534	21.99824701985859
30	19.490102277220426	24.56397249956025	34.58256972455961	21.363355498659704
31	21.039069206633663	23.068827773459642	31.093447672941416	24.798655346965283
32	22.312009417382168	24.0277049039845	31.996247479903122	21.664038198730218
33	19.503031633323463	22.988545492540812	33.363301375773695	24.14512149836203
34	21.774388749656094	25.011238015915133	32.33932644068357	20.8750467937452
35	18.820782586863473	24.09986875200142	33.119898730066616	23.95944993106849
36	20.36959917492667	26.00273921939764	31.790430171704852	21.83723143397083
37	18.80183957675903	25.662065720217754	31.789377782254608	23.746716920768606
38	18.819128832013085	25.214649862512832	31.643697014070447	24.32252429140363
39	21.238572178130447	25.73919083278584	30.557931784115834	22.46430520496788
40	19.747336327130753	23.41190673424009	35.4784538294197	21.36230310920946
41	18.61436391326507	26.811274999887242	32.21995540875558	22.354405678092107
42	19.56933216868901	23.91585093955827	34.578961532158765	21.93585535959396
43	21.35027580120664	23.11633564007078	31.754949613096535	23.778438945626043
44	20.9744224261185	23.380184709382654	31.00489661777065	24.640496246728198
45	20.035841377848403	22.312911465482376	31.69962399628356	25.951623160385655
46	22.496778936575495	24.96583492820449	30.6622686810403	21.875117454179716
47	17.87288037489119	23.939905555563907	36.50678866366084	21.68042540588406
48	20.293376110458798	24.11565459375512	31.631369023367554	23.959600272418527
49	20.13266120727111	21.481523799787418	35.53633524918327	22.849479743758202
50	19.466198002564823	22.207371837757627	33.466585883247916	24.859844276429634
51	19.666903704861888	21.991782341807074	31.627159465566567	26.714154487764468
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	191.0
1	679.5
2	1168.0
3	10032.5
4	18897.0
5	12752.0
6	6607.0
7	6348.5
8	6090.0
9	5932.5
10	5775.0
11	5565.5
12	5356.0
13	5219.0
14	5082.0
15	4781.0
16	4480.0
17	4203.5
18	3927.0
19	3879.0
20	3831.0
21	3670.0
22	3509.0
23	3716.0
24	3923.0
25	4454.5
26	5799.5
27	6613.0
28	7527.5
29	8442.0
30	9849.5
31	11257.0
32	12491.5
33	13726.0
34	15649.5
35	17573.0
36	18929.5
37	20286.0
38	21875.0
39	23464.0
40	25387.5
41	27311.0
42	29540.5
43	31770.0
44	33983.0
45	36196.0
46	60990.0
47	85784.0
48	70794.5
49	55805.0
50	54342.5
51	52880.0
52	47485.5
53	42091.0
54	38687.0
55	35283.0
56	32772.0
57	30261.0
58	28139.5
59	26018.0
60	23971.0
61	21924.0
62	19419.5
63	16915.0
64	14307.5
65	11700.0
66	9297.0
67	6894.0
68	5440.5
69	3987.0
70	3249.0
71	2511.0
72	1956.0
73	1401.0
74	1026.5
75	488.0
76	324.0
77	235.5
78	147.0
79	109.0
80	71.0
81	48.5
82	26.0
83	19.0
84	12.0
85	7.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	665153.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.40150395906014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80289003336344	31.717550378988008
2	8.561285019779906	7.260227213216801
3	3.861878317466621	4.912483463024084
4	2.611078175385744	4.4285456636413665
5	1.8970239590694458	4.021833445545753
6	1.5994347372693969	4.069106300675199
7	1.2700319185782782	3.769588439661073
8	1.0374145023184995	3.5190348101795417
9	0.9019017010491052	3.4417789692945044
>10	3.397972034413202	20.345784583075776
>50	0.033351186557785226	0.9483963727058796
>100	0.018850669956718913	1.5209695016429672
>500	0.002537590186481376	0.6956956002461896
>1k	0.0029001030702644295	2.7932347431890756
>5k	3.625128837830537E-4	1.3730996016348236
>10k+	0.0010875386513491611	5.182670913279004
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	12336	1.8546108940349062	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	10957	1.6472901723362896	RNA PCR Primer, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10424	1.5671582327674987	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	8933	1.3429992798649335	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	3640	0.5472425141283284	Illumina PCR Primer Index 6 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	2652	0.3987052602934964	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	2595	0.39013580334148684	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	2264	0.34037281647981743	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	2078	0.31240932537326	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	1969	0.2960221182194172	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	1817	0.2731702330140584	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	1157	0.17394494199079008	RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	956	0.14372633063370382	RNA PCR Primer, Index 27 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1879266875440688	0.0
2	0.0	0.0	0.0	1.0460751135453046	0.0
3	0.0	0.0	0.0	1.3308216305120777	0.0
4	0.0	0.0	0.0	2.059375812782924	0.0
5	0.0	0.0	0.0	4.50933845295744	0.0
6	0.0	0.0	0.0	5.100330299946028	0.0
7	0.0	0.0	0.0	5.978925149552058	0.0
8	0.0	0.0	0.0	6.967269184683825	0.0
9	0.0	0.0	0.0	7.290503087259623	0.0
10	0.0	0.0	0.0	9.90899838082366	0.0
11	0.0	0.0	0.0	10.992508490527744	0.0
12	0.0	0.0	0.0	13.235601433053748	0.0
13	0.0	0.0	0.0	13.660015064203273	0.0
14	0.0	0.0	0.0	13.860871107850373	0.0
15	0.0	0.0	0.0	14.509894715952571	0.0
16	0.0	0.0	0.0	14.995497276566445	0.0
17	0.0	0.0	0.0	15.587240830305207	0.0
18	0.0	0.0	0.0	16.136437781983993	0.0
19	0.0	0.0	0.0	16.85491909380248	0.0
20	0.0	0.0	0.0	17.29602061480592	0.0
21	0.0	0.0	0.0	17.741031010910273	0.0
22	0.0	0.0	0.0	18.304660732192442	0.0
23	0.0	0.0	0.0	18.77778496075339	0.0
24	0.0	0.0	0.0	19.168973153545124	0.0
25	0.0	0.0	0.0	19.502881291973427	0.0
26	0.0	0.0	0.0	19.846862300854088	0.0
27	0.0	0.0	0.0	20.223617724042438	0.0
28	0.0	0.0	0.0	20.568801463723386	0.0
29	0.0	0.0	0.0	20.92165261225613	0.0
30	0.0	0.0	0.0	21.31509592529839	0.0
31	0.0	0.0	0.0	21.70613377674009	0.0
32	1.5034135003525506E-4	0.0	0.0	22.08318988262851	0.0
33	1.5034135003525506E-4	0.0	0.0	22.449120728614318	0.0
34	1.5034135003525506E-4	0.0	0.0	22.818960449701045	0.0
35	1.5034135003525506E-4	0.0	0.0	23.19376143533894	0.0
36	1.5034135003525506E-4	0.0	0.0	23.576229829828627	0.0
37	1.5034135003525506E-4	0.0	0.0	23.943213065264683	0.0
38	1.5034135003525506E-4	0.0	0.0	24.32959033485529	0.0
39	1.5034135003525506E-4	0.0	0.0	24.738969831001288	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	25	3.8890263E-5	45.000004	1
CACTGCG	25	3.8890263E-5	45.000004	1
GGGTCGA	20	7.031141E-4	45.0	7
ACGGATA	20	7.031141E-4	45.0	5
CGTTTTT	6395	0.0	43.6982	1
ACGGCTG	1190	0.0	41.596638	8
TACGGCT	1205	0.0	41.26556	7
AACGGGC	60	3.6379788E-12	41.250004	4
ACCACCG	1045	0.0	40.47847	14
TGATACC	1425	0.0	40.26316	4
CGGCGAT	95	0.0	40.263157	31
CGGCTGT	1265	0.0	40.197628	9
CGAACCC	45	1.9261279E-8	40.0	34
GCGAACC	45	1.9261279E-8	40.0	33
GATACCT	1435	0.0	39.825787	5
CGACCAC	1460	0.0	39.452057	12
TCACGAC	80	0.0	39.375	25
CGACGGT	80	0.0	39.375	28
CCGTAGG	40	3.4552977E-7	39.375	2
CGATGAA	680	0.0	39.375	19
>>END_MODULE