##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552301_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 407193 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.99989439897051 28.0 16.0 31.0 16.0 33.0 2 26.101600469556207 28.0 23.0 31.0 16.0 33.0 3 26.54833948520726 30.0 23.0 33.0 16.0 34.0 4 30.11500934446319 33.0 28.0 35.0 19.0 37.0 5 32.07320607181361 33.0 32.0 35.0 28.0 37.0 6 30.408233933294532 35.0 28.0 35.0 17.0 37.0 7 32.92710090792327 35.0 32.0 35.0 28.0 37.0 8 33.23356491884684 35.0 33.0 36.0 28.0 37.0 9 35.1154047343643 37.0 34.0 39.0 30.0 39.0 10 34.975785438354784 37.0 34.0 39.0 30.0 39.0 11 34.99227884565796 37.0 34.0 39.0 30.0 39.0 12 34.674851974370874 37.0 33.0 39.0 27.0 39.0 13 35.07219181076295 37.0 34.0 39.0 30.0 39.0 14 35.43114935669326 38.0 34.0 40.0 27.0 41.0 15 35.850134948292336 38.0 34.0 40.0 29.0 41.0 16 35.41492117988276 37.0 33.0 40.0 27.0 41.0 17 35.12799581525223 37.0 33.0 39.0 27.0 41.0 18 35.0946013315553 37.0 33.0 39.0 27.0 40.0 19 34.45391988565619 37.0 32.0 39.0 27.0 40.0 20 35.120650404108126 36.0 34.0 39.0 29.0 40.0 21 35.638687305528336 37.0 34.0 39.0 30.0 40.0 22 36.3617400102654 38.0 35.0 40.0 31.0 40.0 23 36.803641025263204 38.0 35.0 40.0 32.0 41.0 24 36.765843224220454 38.0 35.0 40.0 32.0 41.0 25 35.07584855338869 37.0 34.0 40.0 27.0 41.0 26 35.71904968896813 37.0 34.0 40.0 30.0 41.0 27 36.17942106077462 38.0 35.0 40.0 31.0 41.0 28 36.16099245321015 38.0 35.0 40.0 30.0 41.0 29 36.389336260692104 38.0 35.0 40.0 31.0 41.0 30 35.49120932825466 38.0 34.0 40.0 29.0 41.0 31 35.44763294064485 37.0 34.0 40.0 30.0 41.0 32 35.338488137075046 37.0 34.0 40.0 29.0 41.0 33 35.38746000054029 38.0 35.0 40.0 27.0 41.0 34 35.276947295262936 38.0 35.0 40.0 25.0 41.0 35 35.15134837779628 38.0 35.0 40.0 24.0 41.0 36 34.81144567809368 38.0 34.0 40.0 23.0 41.0 37 34.97456734275884 38.0 35.0 40.0 23.0 41.0 38 34.51901186906455 38.0 34.0 40.0 22.0 41.0 39 34.54784340595246 38.0 34.0 40.0 23.0 41.0 40 34.27445216396156 37.0 34.0 40.0 22.0 41.0 41 33.94717247103953 37.0 33.0 40.0 21.0 41.0 42 34.33990515554049 37.0 34.0 40.0 22.0 41.0 43 34.346771678295056 37.0 34.0 40.0 22.0 41.0 44 34.50038679446847 37.0 34.0 40.0 22.0 41.0 45 34.40205504515058 37.0 34.0 40.0 22.0 41.0 46 34.27306461555086 37.0 34.0 40.0 22.0 41.0 47 34.14777267782108 37.0 34.0 40.0 22.0 41.0 48 34.14683945942096 37.0 34.0 40.0 22.0 41.0 49 34.22244979653383 37.0 34.0 40.0 22.0 41.0 50 34.14502459521652 37.0 34.0 40.0 22.0 41.0 51 32.94143808955459 35.0 32.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 10.0 9 11.0 10 16.0 11 16.0 12 10.0 13 25.0 14 28.0 15 45.0 16 74.0 17 166.0 18 344.0 19 645.0 20 1032.0 21 1596.0 22 2193.0 23 3025.0 24 4324.0 25 6690.0 26 8604.0 27 9510.0 28 9733.0 29 10608.0 30 13109.0 31 18081.0 32 24344.0 33 31971.0 34 37826.0 35 53680.0 36 68822.0 37 66050.0 38 31714.0 39 2889.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33989042051312 17.034673975240242 20.85178281551991 18.77365278872672 2 35.13542718072266 26.27697430947978 21.368982276217913 17.218616233579652 3 26.82290707354989 24.78063228002446 32.52904642270373 15.867414223721921 4 23.603549176925927 20.840485961202674 39.07631025091296 16.479654610958438 5 28.2576075718394 23.690731422200283 30.698219271942296 17.35344173401802 6 21.58853418403558 32.69948157262036 30.041282635015826 15.670701608328239 7 67.03184976166094 4.389319069826839 24.45105883450845 4.127772334003777 8 66.70817032709306 10.041675569079036 17.634880756791006 5.615273347036909 9 62.225038249675215 5.260404771201863 18.67566485671414 13.838892122408783 10 37.77839992337786 21.03842649554388 25.66547067361178 15.517702907466486 11 30.144673410397527 19.33603966669368 31.12357039536534 19.39571652754345 12 23.90267023254329 18.05409228547642 36.16073950190696 21.88249798007333 13 21.67497967794142 20.724815996345715 40.12323394557372 17.476970380139146 14 19.627056457257368 23.814014484531906 36.24006306591714 20.31886599229358 15 17.585027247521445 19.998870314568276 42.546409196621745 19.86969324128853 16 20.07475570552539 21.2884307932602 34.29651295577282 24.340300545441597 17 19.32228697448139 22.50554405404808 36.89307036221153 21.279098609259 18 22.01928815082774 19.57622061282979 37.35624138921838 21.04824984712409 19 19.59513056462169 23.32235573794245 33.9146301630922 23.167883534343662 20 23.185811150977546 21.689469121522226 38.08169590341681 17.04302382408342 21 21.134940924819436 27.179494735911476 34.237327262502056 17.448237076767036 22 20.18944333522433 20.606690193593703 36.35229485772103 22.851571613460937 23 20.862342918468638 25.098172119854713 34.037667641634314 20.00181732004234 24 22.18014553295366 21.205423472407432 32.96864140591808 23.645789588720827 25 17.729676099540022 27.03263562978735 31.654031380696622 23.583656889976005 26 19.548960812194707 21.399680249906066 35.61701699194239 23.43434194595683 27 21.892075747864034 22.790666833663646 32.06734889843391 23.24990852003841 28 16.30799154209429 24.16790072520893 37.79583637243273 21.728271360264053 29 24.37247202186678 20.03104179099346 34.38271286588915 21.213773321250613 30 22.75137342734281 22.94612137242045 35.46622854518619 18.836276655050554 31 24.471442289037384 21.016815122067424 33.478964520509926 21.032778068385262 32 25.292920064932357 23.083648294543373 32.257921919089966 19.365509721434307 33 23.143079571603636 20.698538530868653 31.832325211877414 24.326056685650293 34 23.361403560473782 21.242506624622724 35.642803289840444 19.753286525063054 35 23.104031749072306 23.236401411615624 30.66211845488503 22.99744838442704 36 25.097680952275702 24.476353964827492 32.04401843843092 18.38194664446589 37 23.737392342206277 22.65854275490983 34.25206228987237 19.35200261301152 38 23.05417823980275 25.758546929834253 30.83893878332879 20.348336047034206 39 22.97067975137097 21.641825866358214 34.825746022156565 20.561748360114247 40 24.680434093906328 20.703695790448265 30.981868548820827 23.634001566824576 41 20.033988796467522 23.391119199003914 32.97036049244462 23.604531512083952 42 21.05144243638766 20.51435068873974 33.142514728887775 25.29169214598483 43 23.81008514389982 20.643036594440474 32.08404859612027 23.462829665539438 44 23.567448359868663 20.695837109184097 32.37187279742039 23.364841733526852 45 22.62023168374702 20.7336570127679 31.05480693430388 25.591304369181202 46 24.58416524842028 23.513419926177512 31.450442419196794 20.451972406205414 47 19.639090062943126 22.401907694876876 37.529623544609066 20.42937869757093 48 21.932105905553385 22.915668982521805 32.4804208323817 22.671804279543114 49 22.177689695058607 19.699749258950916 35.87021387892228 22.252347167068194 50 21.39918908232705 20.70025761739519 33.06466466761462 24.835888632663135 51 20.776143008352303 19.629021127573413 31.280744020648687 28.314091843425597 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 253.0 1 640.5 2 1028.0 3 7022.0 4 13016.0 5 8623.0 6 4230.0 7 4010.5 8 3791.0 9 3740.0 10 3689.0 11 3647.5 12 3606.0 13 3399.0 14 3192.0 15 3068.0 16 2944.0 17 2823.5 18 2703.0 19 2528.0 20 2353.0 21 2203.0 22 2053.0 23 2118.5 24 2184.0 25 2299.5 26 2701.0 27 2987.0 28 3284.0 29 3581.0 30 3908.5 31 4236.0 32 4865.0 33 5494.0 34 6063.5 35 6633.0 36 7348.5 37 8064.0 38 8630.5 39 9197.0 40 10292.5 41 11388.0 42 13449.0 43 15510.0 44 17501.0 45 19492.0 46 35875.0 47 52258.0 48 44106.0 49 35954.0 50 36692.5 51 37431.0 52 33044.0 53 28657.0 54 26430.5 55 24204.0 56 22198.5 57 20193.0 58 19262.0 59 18331.0 60 17230.0 61 16129.0 62 14842.5 63 13556.0 64 11887.0 65 10218.0 66 8457.5 67 6697.0 68 5432.5 69 4168.0 70 3346.5 71 2525.0 72 2039.0 73 1553.0 74 1170.0 75 530.0 76 273.0 77 191.5 78 110.0 79 86.0 80 62.0 81 42.0 82 22.0 83 15.5 84 9.0 85 9.0 86 9.0 87 7.0 88 5.0 89 3.0 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 407193.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.60008521276635 #Duplication Level Percentage of deduplicated Percentage of total 1 76.4775285245707 32.57949232008481 2 8.424122275764224 7.177366535806384 3 3.500711103966895 4.473917740028012 4 2.236230474184596 3.810544350225948 5 1.6495537262052897 3.5135564649690796 6 1.3536241254893715 3.4598701855142133 7 1.1892369337755904 3.546311630190639 8 0.957002117840047 3.2614697415027094 9 0.7756086893429014 2.973689663199266 >10 3.354800196266458 21.105601339211002 >50 0.0501145543077046 1.4407986492793212 >100 0.023891822402510327 1.950440211472713 >500 0.0017481821270129508 0.5995052960044039 >1k 0.0034963642540259015 2.911138139231323 >5k 0.0023309095026839343 7.196297733280193 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG 7936 1.9489529535134447 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC 7740 1.900818530770421 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6980 1.714174850746452 No Hit GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC 6333 1.555282138936573 No Hit GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC 3743 0.9192201241180472 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT 2265 0.5562472832293286 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA 1708 0.41945711247491974 No Hit GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT 1536 0.3772167006800215 No Hit GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT 1405 0.3450452242548374 No Hit CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC 1070 0.2627746547705879 No Hit CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT 816 0.20039637223626142 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC 810 0.1989228694992301 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC 789 0.1937656099196204 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATG 496 0.12180955959459029 No Hit GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTTGTCT 445 0.10928478632982394 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGT 443 0.10879361875081349 No Hit TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC 426 0.10461869432922472 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.21046530760597554 0.0 2 7.36751368515667E-4 0.0 0.0 1.642464384210927 0.0 3 7.36751368515667E-4 0.0 0.0 1.8971347739278426 0.0 4 7.36751368515667E-4 0.0 0.0 2.607608676966451 0.0 5 7.36751368515667E-4 0.0 0.0 5.012610727591094 0.0 6 7.36751368515667E-4 0.0 0.0 5.510163485128674 0.0 7 7.36751368515667E-4 0.0 0.0 6.354726137237133 0.0 8 7.36751368515667E-4 0.0 0.0 7.248896714825648 0.0 9 7.36751368515667E-4 0.0 0.0 7.61064163676684 0.0 10 7.36751368515667E-4 0.0 0.0 10.610693209362637 0.0 11 7.36751368515667E-4 0.0 0.0 12.171869359247335 0.0 12 7.36751368515667E-4 0.0 0.0 15.354144103656006 0.0 13 7.36751368515667E-4 0.0 0.0 15.979154847946797 0.0 14 7.36751368515667E-4 0.0 0.0 16.224493053662513 0.0 15 7.36751368515667E-4 0.0 0.0 16.99685407165644 0.0 16 7.36751368515667E-4 0.0 0.0 17.678103503743923 0.0 17 7.36751368515667E-4 0.0 0.0 18.434255991630504 0.0 18 7.36751368515667E-4 0.0 0.0 19.14325639193208 0.0 19 7.36751368515667E-4 0.0 0.0 20.2351219200723 0.0 20 7.36751368515667E-4 0.0 0.0 20.82575093383236 0.0 21 7.36751368515667E-4 0.0 0.0 21.355475167795124 0.0 22 7.36751368515667E-4 0.0 0.0 21.973855149769275 0.0 23 7.36751368515667E-4 0.0 0.0 22.507508724364122 0.0 24 7.36751368515667E-4 0.0 0.0 22.97387234063454 0.0 25 7.36751368515667E-4 0.0 0.0 23.341511273523857 0.0 26 7.36751368515667E-4 0.0 0.0 23.722411731046456 0.0 27 7.36751368515667E-4 0.0 0.0 24.143096762468904 0.0 28 7.36751368515667E-4 0.0 0.0 24.537749912203797 0.0 29 7.36751368515667E-4 0.0 0.0 24.91840478593689 0.0 30 7.36751368515667E-4 0.0 0.0 25.37224362894254 0.0 31 7.36751368515667E-4 0.0 0.0 25.76959820036199 0.0 32 7.36751368515667E-4 0.0 0.0 26.146078149673496 0.0 33 7.36751368515667E-4 0.0 0.0 26.582726127413782 0.0 34 7.36751368515667E-4 0.0 0.0 26.993342223466513 0.0 35 7.36751368515667E-4 0.0 0.0 27.423605022679663 0.0 36 7.36751368515667E-4 0.0 0.0 27.8062245667288 0.0 37 7.36751368515667E-4 0.0 0.0 28.207754062569837 0.0 38 7.36751368515667E-4 0.0 0.0 28.652506305363797 0.0 39 7.36751368515667E-4 0.0 0.0 29.133113781425514 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATCG 25 3.8863524E-5 45.000004 21 TTATGCG 25 3.8863524E-5 45.000004 1 TGCGGTC 25 3.8863524E-5 45.000004 26 TAACGCC 25 3.8863524E-5 45.000004 12 TACCGAT 20 7.0279156E-4 45.0 30 CGTTTTT 3955 0.0 43.2933 1 TACGGCT 990 0.0 42.045452 7 AACACGT 75 0.0 42.0 41 ACGGGCT 135 0.0 41.666668 5 TACGGGT 65 0.0 41.53846 4 ACGGGCC 65 0.0 41.53846 5 GTGTACG 50 1.0786607E-9 40.500004 1 GTGTAAG 50 1.0786607E-9 40.500004 1 GTCAAGC 95 0.0 40.26316 16 TATGCGG 45 1.9230356E-8 40.000004 2 GACCTAT 45 1.9230356E-8 40.000004 33 CGACCAC 855 0.0 40.0 12 ACGGCTG 1040 0.0 39.80769 8 TGATCCG 85 0.0 39.705883 4 ATCCGGC 85 0.0 39.705883 6 >>END_MODULE