FastQCFastQC Report
Sat 18 Jun 2016
SRR3552300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438460
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG106302.424394471559549No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC102842.3454819139716276No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC94052.145007526342198No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76621.7474798157186517No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC39650.9043014186014688No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT20750.47324727455184057No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT20570.46914199698946313No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA16650.3797381745199106No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC11900.2714044610682845No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC10780.24586051179126944No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT10260.23400082105551248No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT8960.20435159421612006No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC7380.16831638005747387No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATG4900.11175477808694066No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGT4680.10673721662181272No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC4390.10012315832687133No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA207.028507E-445.0000041
CGCATCG207.028507E-445.00000421
TAACGCC207.028507E-445.00000412
TATTGCG207.028507E-445.0000041
GTAGACG207.028507E-445.0000041
CTACGGG351.209628E-745.0000043
TACGACC207.028507E-445.0000046
TACGAAG207.028507E-445.0000041
TCATGCG207.028507E-445.0000041
CATACGC207.028507E-445.00000422
CGTACAA207.028507E-445.00000417
CGGCTAG207.028507E-445.0000043
ATAACGC207.028507E-445.00000411
CGCACGG406.7975634E-945.0000042
TCGGTAT207.028507E-445.00000433
TCTATTG207.028507E-445.0000041
CGAATAT551.8189894E-1245.014
AATACGG253.886846E-545.02
CGCCCGA253.886846E-545.035
TATGACG302.16242E-644.9999961