##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438460
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.89407699676139	30.0	25.0	31.0	16.0	33.0
2	28.523527801851937	31.0	27.0	33.0	16.0	34.0
3	28.432349587191535	31.0	27.0	33.0	16.0	34.0
4	32.69627560096702	35.0	32.0	35.0	28.0	37.0
5	33.75785248369292	35.0	33.0	35.0	30.0	37.0
6	33.444485243807875	35.0	33.0	36.0	28.0	37.0
7	34.37680518177257	35.0	35.0	37.0	32.0	37.0
8	34.335189070838844	35.0	35.0	37.0	31.0	37.0
9	36.17539342243306	39.0	35.0	39.0	30.0	39.0
10	36.02835606440724	37.0	35.0	39.0	31.0	39.0
11	36.08790083473977	37.0	35.0	39.0	31.0	39.0
12	35.86432057656343	37.0	35.0	39.0	31.0	39.0
13	36.06932217306026	37.0	35.0	39.0	32.0	39.0
14	37.148741048214205	39.0	36.0	40.0	32.0	41.0
15	37.09719928841856	39.0	36.0	40.0	32.0	41.0
16	36.921529900104915	38.0	35.0	40.0	32.0	41.0
17	35.88772522008849	38.0	34.0	40.0	29.0	41.0
18	35.7015075491493	38.0	34.0	39.0	29.0	41.0
19	35.24687542763308	37.0	33.0	39.0	28.0	40.0
20	35.72597956484058	37.0	34.0	39.0	30.0	40.0
21	36.2874446927884	38.0	35.0	39.0	31.0	40.0
22	36.74966473566574	38.0	35.0	40.0	32.0	41.0
23	36.98812206358619	38.0	35.0	40.0	32.0	41.0
24	36.94622086393286	38.0	35.0	40.0	33.0	41.0
25	36.304335629247824	38.0	35.0	40.0	31.0	41.0
26	36.517130410983896	38.0	35.0	40.0	31.0	41.0
27	36.85117684623455	38.0	35.0	40.0	33.0	41.0
28	36.70166947954203	38.0	35.0	40.0	32.0	41.0
29	36.78593258221959	39.0	35.0	40.0	32.0	41.0
30	36.434979245541214	38.0	35.0	40.0	31.0	41.0
31	36.22440587510833	38.0	35.0	40.0	30.0	41.0
32	36.10076631847831	38.0	35.0	40.0	30.0	41.0
33	35.78806048442275	38.0	35.0	40.0	29.0	41.0
34	35.45876020617616	38.0	35.0	40.0	25.0	41.0
35	35.29080873967979	39.0	35.0	40.0	24.0	41.0
36	34.96123021484286	38.0	35.0	40.0	23.0	41.0
37	34.986532408885644	38.0	35.0	40.0	23.0	41.0
38	34.58462801623865	38.0	34.0	40.0	21.0	41.0
39	34.6452561237057	38.0	34.0	40.0	22.0	41.0
40	34.442101902111936	38.0	34.0	40.0	21.0	41.0
41	34.187852939834876	38.0	34.0	40.0	19.0	41.0
42	34.32062217762168	38.0	34.0	40.0	20.0	41.0
43	34.27290516808831	38.0	34.0	40.0	20.0	41.0
44	34.182819413401454	38.0	34.0	40.0	19.0	41.0
45	34.01658075993249	37.0	34.0	40.0	20.0	41.0
46	33.87098481047302	37.0	33.0	40.0	20.0	41.0
47	33.8471650777722	37.0	33.0	40.0	20.0	41.0
48	33.74046207179674	37.0	33.0	40.0	20.0	41.0
49	33.730741686812934	37.0	33.0	40.0	20.0	41.0
50	33.628506591251195	36.0	33.0	40.0	19.0	41.0
51	31.493614012680748	35.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	15.0
10	20.0
11	20.0
12	14.0
13	24.0
14	27.0
15	38.0
16	89.0
17	208.0
18	372.0
19	543.0
20	911.0
21	1304.0
22	2079.0
23	2850.0
24	4368.0
25	6745.0
26	9210.0
27	10662.0
28	10589.0
29	10403.0
30	11581.0
31	13765.0
32	18370.0
33	26476.0
34	36485.0
35	41932.0
36	55687.0
37	78569.0
38	75555.0
39	19537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.744469278839574	16.90188386625918	20.463440222597274	18.890206632303975
2	34.14222506043881	29.733841171372532	20.86279250102632	15.26114126716234
3	25.8890206632304	28.060712493728047	32.24877982028007	13.801487022761483
4	22.964466541987864	20.26821146740866	40.72686219951649	16.040459791086985
5	28.199151575970443	23.337818729188523	30.76152898782101	17.701500707020024
6	20.82903799662455	34.78310450212106	30.676230442913834	13.711627058340556
7	66.22930255895635	4.583086256443005	24.98084203804224	4.206769146558409
8	65.06135109245997	10.842038042238745	18.726907813711627	5.369703051589655
9	60.97659991789445	5.561738813118643	19.227067463394608	14.234593805592299
10	36.72284815034439	20.88103817908133	26.48907540026456	15.90703827030972
11	29.21908497924554	19.092505587738902	31.917620763581628	19.770788669433927
12	23.821557268621994	17.91748392099621	36.95890161018109	21.302057200200704
13	20.430369931122566	20.68033572047621	41.81955024403594	17.069744104365277
14	19.635542580851162	23.913926013775484	36.045021210600744	20.405510194772614
15	17.117182867308305	19.960999863157415	44.30506773707978	18.6167495324545
16	19.499384208365644	21.6838480135018	34.18396204898965	24.63280572914291
17	18.970943757697395	21.518268485152582	37.8882908361082	21.62249692104183
18	21.5344615244264	20.076859918806733	37.70993933312047	20.678739223646396
19	19.102312639693473	23.691556812480044	34.26082196779638	22.945308580030105
20	22.051954568261642	22.157551430005018	38.830223965698124	16.96027003603521
21	20.439036628198696	27.637640833827486	34.67249007891256	17.25083245906126
22	19.888473292888747	20.396159284769418	35.77772202709483	23.937645395247
23	20.052684395383842	25.921406741778043	34.68868311818638	19.337225744651736
24	22.313551977375358	20.52615974091137	32.63878118870592	24.521507093007344
25	17.22962185832231	28.624275874652195	31.524654472471838	22.621447794553664
26	19.56324408155818	21.038407152305798	35.19249190348036	24.205856862655658
27	21.907813711627057	23.141221548145783	31.475391141723303	23.475573598503853
28	16.068740592072253	23.031747479815717	38.660311088810836	22.23920083930119
29	25.31017652693518	19.70898143502258	33.41627514482507	21.56456689321717
30	23.197783150116315	22.598184555033527	35.63221274460612	18.571819550244037
31	24.756876339916982	20.50335264334261	33.88678556766866	20.852985449071753
32	25.314053733521874	23.002098252976328	32.8034484331524	18.880399580349405
33	23.087624868859187	20.29900104912649	31.963919171646214	24.649454910368107
34	24.062628289923822	20.712265657072482	36.263285134333806	18.96182091866989
35	23.096519636911005	22.73753592117867	30.216667426903253	23.94927701500707
36	25.446791041372073	23.72143411029512	32.979747297358934	17.85202755097386
37	23.722346394197874	22.365096017880763	34.81457829676595	19.097979291155408
38	23.137344341559093	25.187930483966607	31.000319299365962	20.674405875108334
39	23.253432468184098	21.125986406969847	35.8096519636911	19.810929161154952
40	24.987684167312867	20.484422752360533	31.20239018382521	23.32550289650139
41	19.543401906673356	23.793504538612417	33.51434566437075	23.148747890343476
42	20.908862838115223	20.234685033982576	34.093417871641655	24.76303425626055
43	23.818136203986683	20.379510103544224	32.29279751858778	23.509556173881315
44	23.266204442822605	20.60621265337773	32.63057063358117	23.49701227021849
45	22.116954796332617	20.343702960361266	31.087898554030012	26.451443689276104
46	25.236737672763766	23.7070656388268	30.906354057382657	20.149842631026775
47	19.024996578935365	21.492724535875567	39.07448798065958	20.40779090452949
48	22.23144642612781	23.02672991835059	31.886146968936735	22.855676686584868
49	21.622724992017517	19.217488482415728	37.07704237558728	22.082744149979476
50	21.510514071979202	20.23514117593395	33.30041508917575	24.953929662911097
51	20.69516033389591	19.280892213656887	30.767914975140265	29.25603247730694
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	304.0
1	729.5
2	1155.0
3	7777.5
4	14400.0
5	9491.5
6	4583.0
7	4386.5
8	4190.0
9	4163.5
10	4137.0
11	4081.5
12	4026.0
13	3853.5
14	3681.0
15	3555.5
16	3430.0
17	3226.5
18	3023.0
19	2904.5
20	2786.0
21	2574.5
22	2363.0
23	2348.0
24	2333.0
25	2432.5
26	2871.0
27	3210.0
28	3403.0
29	3596.0
30	4086.5
31	4577.0
32	5250.0
33	5923.0
34	6540.5
35	7158.0
36	8053.0
37	8948.0
38	9457.0
39	9966.0
40	11239.0
41	12512.0
42	14674.5
43	16837.0
44	19256.5
45	21676.0
46	43260.5
47	64845.0
48	50769.5
49	36694.0
50	37846.5
51	38999.0
52	34397.5
53	29796.0
54	27059.0
55	24322.0
56	22326.5
57	20331.0
58	19509.5
59	18688.0
60	17715.5
61	16743.0
62	15324.0
63	13905.0
64	12159.5
65	10414.0
66	8550.5
67	6687.0
68	5481.5
69	4276.0
70	3445.5
71	2615.0
72	2063.5
73	1512.0
74	1109.5
75	491.0
76	275.0
77	207.5
78	140.0
79	106.5
80	73.0
81	56.5
82	40.0
83	29.5
84	19.0
85	16.5
86	14.0
87	11.0
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	438460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.9219458681319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.32468232379792	30.47029836133025
2	8.27036634519851	6.603382350852698
3	3.251914926779308	3.894683150239611
4	2.081725235960641	3.324260887293793
5	1.5930499822328223	3.179882757796361
6	1.3349252634041089	3.1975688462172336
7	1.196751176436047	3.3443644978312133
8	0.9992013638524672	3.191205020726542
9	0.8071095893661608	2.8999246802693444
>10	4.05262384536492	24.27483957487782
>50	0.05362792847011344	1.4735592450153203
>100	0.02652564203898083	2.0207628577947947
>500	0.0011532887843035144	0.3761593198492475
>1k	0.0040365107450623	3.0055912362005968
>5k	0.0011532887843035144	3.9289541688293186
>10k+	0.0011532887843035144	4.8145630448758645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	10630	2.424394471559549	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	10284	2.3454819139716276	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	9405	2.145007526342198	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7662	1.7474798157186517	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	3965	0.9043014186014688	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT	2075	0.47324727455184057	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT	2057	0.46914199698946313	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA	1665	0.3797381745199106	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC	1190	0.2714044610682845	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1078	0.24586051179126944	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT	1026	0.23400082105551248	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	896	0.20435159421612006	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC	738	0.16831638005747387	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATG	490	0.11175477808694066	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGT	468	0.10673721662181272	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	439	0.10012315832687133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.28070975687634E-4	0.0	0.0	0.22556219495507002	0.0
2	4.56141951375268E-4	0.0	0.0	1.6854445103316151	0.0
3	4.56141951375268E-4	0.0	0.0	1.9520594809104594	0.0
4	4.56141951375268E-4	0.0	0.0	2.7439219084979247	0.0
5	4.56141951375268E-4	0.0	0.0	5.847055603703873	0.0
6	4.56141951375268E-4	0.0	0.0	6.3670574282716785	0.0
7	4.56141951375268E-4	0.0	0.0	7.372622360078457	0.0
8	4.56141951375268E-4	0.0	0.0	8.406924234821876	0.0
9	4.56141951375268E-4	0.0	0.0	8.7440131368882	0.0
10	4.56141951375268E-4	0.0	0.0	12.264972859553893	0.0
11	4.56141951375268E-4	0.0	0.0	13.785750125439037	0.0
12	4.56141951375268E-4	0.0	0.0	17.48528942206815	0.0
13	4.56141951375268E-4	0.0	0.0	18.098800346667883	0.0
14	4.56141951375268E-4	0.0	0.0	18.364274962368288	0.0
15	4.56141951375268E-4	0.0	0.0	19.101172284815036	0.0
16	4.56141951375268E-4	0.0	0.0	19.79177119919719	0.0
17	4.56141951375268E-4	0.0	0.0	20.51201934041874	0.0
18	4.56141951375268E-4	0.0	0.0	21.217670939196278	0.0
19	4.56141951375268E-4	0.0	0.0	21.977375359211788	0.0
20	4.56141951375268E-4	0.0	0.0	22.494184190119967	0.0
21	4.56141951375268E-4	0.0	0.0	23.018519363225835	0.0
22	4.56141951375268E-4	0.0	0.0	23.598047712448114	0.0
23	4.56141951375268E-4	0.0	0.0	24.089540665054965	0.0
24	4.56141951375268E-4	0.0	0.0	24.535647493499976	0.0
25	4.56141951375268E-4	0.0	0.0	24.8930347124025	0.0
26	4.56141951375268E-4	0.0	0.0	25.270264106189845	0.0
27	6.842129270629019E-4	0.0	0.0	25.654791771199196	0.0
28	6.842129270629019E-4	0.0	0.0	26.051407197919993	0.0
29	6.842129270629019E-4	0.0	0.0	26.431145372439904	0.0
30	6.842129270629019E-4	0.0	0.0	26.89093645942617	0.0
31	9.12283902750536E-4	0.0	0.0	27.286867673219906	0.0
32	9.12283902750536E-4	0.0	0.0	27.656342653833875	0.0
33	9.12283902750536E-4	0.0	0.0	28.072572184463805	0.0
34	9.12283902750536E-4	0.0	0.0	28.45687177849747	0.0
35	9.12283902750536E-4	0.0	0.0	28.858960908634767	0.0
36	9.12283902750536E-4	0.0	0.0	29.24166400583862	0.0
37	9.12283902750536E-4	0.0	0.0	29.622770606212654	0.0
38	9.12283902750536E-4	0.0	0.0	30.062947589289788	0.0
39	9.12283902750536E-4	0.0	0.0	30.50015964968298	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCTA	20	7.028507E-4	45.000004	1
CGCATCG	20	7.028507E-4	45.000004	21
TAACGCC	20	7.028507E-4	45.000004	12
TATTGCG	20	7.028507E-4	45.000004	1
GTAGACG	20	7.028507E-4	45.000004	1
CTACGGG	35	1.209628E-7	45.000004	3
TACGACC	20	7.028507E-4	45.000004	6
TACGAAG	20	7.028507E-4	45.000004	1
TCATGCG	20	7.028507E-4	45.000004	1
CATACGC	20	7.028507E-4	45.000004	22
CGTACAA	20	7.028507E-4	45.000004	17
CGGCTAG	20	7.028507E-4	45.000004	3
ATAACGC	20	7.028507E-4	45.000004	11
CGCACGG	40	6.7975634E-9	45.000004	2
TCGGTAT	20	7.028507E-4	45.000004	33
TCTATTG	20	7.028507E-4	45.000004	1
CGAATAT	55	1.8189894E-12	45.0	14
AATACGG	25	3.886846E-5	45.0	2
CGCCCGA	25	3.886846E-5	45.0	35
TATGACG	30	2.16242E-6	44.999996	1
>>END_MODULE