FastQCFastQC Report
Sat 18 Jun 2016
SRR3552295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008989
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC273502.7106341099853415No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG270192.6778289951624847No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243962.4178658042852796No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC175201.736391576122237No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC83040.8230020347099919Illumina PCR Primer Index 11 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT76260.7558060593326588Illumina PCR Primer Index 11 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT54040.5355856208541421No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA53940.5345945297718806No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC48460.48028273846394753No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT40040.396832869337525No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC38770.3842460125928033No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT25240.25015138916281543Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC19840.1966324707206917Illumina PCR Primer Index 11 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT16770.1662059744952621No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT15690.15550219080683736No Hit
CGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTG15540.15401555418344504Illumina PCR Primer Index 11 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG15320.1518351538024696No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT14820.14687969839116186No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC13320.13201333215723857No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTAACACCTCGT13200.13082402285852474No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATG12880.12765253139528776No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC11560.11457012910943529No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGT11190.11090309210506755No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC10660.10565030936908132No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTAT10430.10337079987987977No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCCC207.032875E-445.00000421
CCGTACG207.032875E-445.0000041
TACGTTG207.032875E-445.0000041
TACCGCC207.032875E-445.00000415
GCGTACG302.1652377E-644.9999961
CGTTTTT141400.043.758841
TACGGCT20900.042.846897
TGTAGCG700.041.7857131
ACGGCTG21500.041.5465138
TGATACC30950.040.783524
GATACCT31350.040.3349275
CCGATGA6900.040.10869618
AATGATC8050.039.409942
ATGATAC86850.039.3782353
TTGCACG403.4576624E-739.3750041
GGCGACT6700.039.29104610
GAATGAT91150.039.2484931
CGATGAA6950.039.1726619
CGACCAC20400.039.1544112
GAATCTG32650.039.1424181
Produced by FastQC (version 0.11.5)