FastQCFastQC Report
Sat 18 Jun 2016
SRR3552291_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552291_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950771
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328093.4507783682926805No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC185081.9466306818361097No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG158731.669487184611226No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC127171.3375460547282152No Hit
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT57780.6077173157363865TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC55710.5859455115900674TruSeq Adapter, Index 19 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA44470.46772566685353256No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC43820.4608891099959927No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC32840.3454038880024738No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT32740.34435211002439076No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT31710.3335187968501353No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG17280.18174723461275114No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT16610.1747003221595947TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC16370.17217605501219538No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT14810.15576831855409978No Hit
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC12550.13199813624942283TruSeq Adapter, Index 19 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT11780.12389944581818334No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT11080.116536999971602No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGAACACTCGT10860.11422308841981929No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGT10670.1122247102614615No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC10100.1062295757863881No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATG9660.10160175268282268No Hit
CGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG9600.10097068589597284TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG406.8121153E-945.01
ACGTTAG207.0326723E-445.01
ATCAGCG207.0326723E-445.01
TAGTGCG406.8121153E-945.01
CGTTTTT186250.044.1664431
TACGGCT14100.040.053197
GCGTAAG451.927765E-840.01
TACGAAT850.039.70588312
CGAATAT850.039.70588314
CTAGCGG1250.039.62
CGTTTTC1650.039.5454521
ACGTACG403.4573895E-739.3751
GCTATCG356.247148E-638.571431
CTAAGCG356.247148E-638.571431
AGACGTT356.247148E-638.5714311
GTATGCG356.247148E-638.571431
CGAATTG356.247148E-638.5714332
GTTTTTT217300.038.569952
TGATACC18800.038.297874
ACGGCTG14900.038.2046978
Produced by FastQC (version 0.11.5)