##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552291_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	950771
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.687028737729694	28.0	16.0	31.0	16.0	33.0
2	26.675483370864278	30.0	25.0	33.0	16.0	33.0
3	26.953005508161272	31.0	25.0	33.0	16.0	33.0
4	29.452053123202116	32.0	28.0	35.0	19.0	37.0
5	32.0870283170185	33.0	32.0	35.0	28.0	37.0
6	30.93101388241753	35.0	30.0	35.0	17.0	37.0
7	33.219859461426566	35.0	32.0	35.0	28.0	37.0
8	33.614731623072224	35.0	33.0	37.0	28.0	37.0
9	35.29482809214837	37.0	34.0	39.0	30.0	39.0
10	35.33211677680535	37.0	34.0	39.0	30.0	39.0
11	35.59826498704735	37.0	34.0	39.0	30.0	39.0
12	35.41269033237236	37.0	34.0	39.0	30.0	39.0
13	35.64251539014126	37.0	34.0	39.0	31.0	39.0
14	35.952905589253355	38.0	34.0	40.0	27.0	41.0
15	36.45917681544768	38.0	34.0	40.0	30.0	41.0
16	36.165563526863984	38.0	34.0	40.0	28.0	41.0
17	35.81353448937757	38.0	34.0	40.0	29.0	41.0
18	35.64970744795539	38.0	34.0	39.0	27.0	40.0
19	34.97856581658465	37.0	33.0	39.0	27.0	40.0
20	35.189230634926815	35.0	34.0	39.0	30.0	40.0
21	35.58971929097543	37.0	34.0	39.0	30.0	40.0
22	36.2264898698004	37.0	35.0	40.0	31.0	40.0
23	36.65525768034574	38.0	35.0	40.0	32.0	41.0
24	36.564811084898466	38.0	35.0	40.0	32.0	41.0
25	34.883410411129496	36.0	34.0	39.0	27.0	41.0
26	35.43305275402805	37.0	34.0	39.0	30.0	41.0
27	36.0657981785309	37.0	35.0	40.0	31.0	41.0
28	36.05188420765884	38.0	35.0	40.0	31.0	41.0
29	36.191366796000295	38.0	35.0	40.0	31.0	41.0
30	35.22286544288793	37.0	34.0	40.0	28.0	41.0
31	35.13669011780965	37.0	34.0	40.0	29.0	41.0
32	34.82674587256027	37.0	34.0	40.0	26.0	41.0
33	34.48025970501835	38.0	34.0	40.0	22.0	41.0
34	34.09436762374957	38.0	34.0	40.0	18.0	41.0
35	33.73074063049883	38.0	33.0	40.0	15.0	41.0
36	33.47279418492992	38.0	33.0	40.0	12.0	41.0
37	33.494701668435404	38.0	33.0	40.0	10.0	41.0
38	33.20556474692644	38.0	33.0	40.0	10.0	41.0
39	33.08441359696499	38.0	33.0	40.0	10.0	41.0
40	32.735184392456226	37.0	32.0	40.0	10.0	41.0
41	32.552912320632416	37.0	32.0	40.0	10.0	41.0
42	32.850485553303585	37.0	33.0	40.0	10.0	41.0
43	32.89143442532429	37.0	33.0	40.0	10.0	41.0
44	32.979888953281076	38.0	33.0	40.0	10.0	41.0
45	33.02579275135653	38.0	33.0	40.0	10.0	41.0
46	32.86749385498716	37.0	33.0	40.0	10.0	41.0
47	32.80532641403661	37.0	33.0	40.0	10.0	41.0
48	32.836718831348456	37.0	33.0	40.0	10.0	41.0
49	32.91821058909033	37.0	33.0	40.0	10.0	41.0
50	32.89046258247254	37.0	33.0	40.0	10.0	41.0
51	31.913042152106026	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	18.0
10	30.0
11	40.0
12	39.0
13	38.0
14	56.0
15	107.0
16	217.0
17	401.0
18	831.0
19	1527.0
20	2663.0
21	4093.0
22	6180.0
23	9193.0
24	14334.0
25	24418.0
26	32397.0
27	33513.0
28	30269.0
29	27748.0
30	29541.0
31	36159.0
32	47764.0
33	61406.0
34	79878.0
35	121441.0
36	150674.0
37	150813.0
38	77025.0
39	7946.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.62167756483949	15.140028461112088	18.829350074834004	28.408943899214428
2	42.48972675859907	23.645967325465335	18.58849291785298	15.275812998082609
3	23.638394524023134	22.673388229131934	39.03726554554146	14.65095170130347
4	21.064904167249527	18.537797219309383	45.48024708368261	14.91705152975848
5	24.12589361686463	21.72615698207034	38.25789806378192	15.89005133728311
6	19.60703471182861	27.82531229917614	38.83742772970568	13.730225259289567
7	59.86152291140559	3.8366757084513514	32.30704344158583	3.994757938557234
8	59.35835232669066	8.08186198359016	27.246203344443614	5.313582345275571
9	54.43918672319623	5.315370367838312	28.11413053195775	12.131312377007712
10	30.39122985450755	22.305055581207252	34.306157844528286	12.997556719756915
11	22.906357051277332	18.358469074046223	42.10225175147328	16.63292212320317
12	19.639745006946992	16.787638663779187	45.09024780940942	18.482368519864405
13	19.126582531440274	18.2188981363546	47.851690890866465	14.802828441338661
14	16.15993756645922	22.585669945759808	43.28487091002986	17.96952157775111
15	14.236866711332171	20.157850838950704	49.16062858459082	16.444653865126302
16	16.960656141173846	21.56555048481706	41.680488782261975	19.79330459174712
17	17.25504879723929	21.09572126200736	43.362912836003616	18.286317104749724
18	17.548600030922273	20.325188715263717	43.96463501726494	18.161576236549074
19	16.971594632145912	22.43495016150051	40.91668761457806	19.67676759177552
20	18.253291276237917	22.590823657852415	44.33191588721154	14.82396917869813
21	17.48118106252715	24.04890346886895	42.64749345531153	15.82242201329237
22	16.695818446292535	19.364705065678276	43.41402924573846	20.52544724229073
23	16.59305973783382	23.234406602641435	42.99773552201319	17.17479813751156
24	18.0732268863901	21.110551331498332	40.65048260832525	20.165739173786328
25	16.05644261341585	24.891903518302513	39.47417411763716	19.57747975064448
26	16.20211386338035	20.834775145644954	41.53586931027555	21.42724168069914
27	17.907571854842015	21.337840552562078	40.77743221027987	19.977155382316035
28	15.03558690788844	21.24717729085132	42.62719414033453	21.090041660925714
29	17.962264309702334	20.135132434624108	40.23524066257805	21.6673625930955
30	17.63179566898864	22.2182838980154	42.529273610575	17.620646822420962
31	20.209387959876775	21.456165575096424	37.79122417490647	20.543222290120333
32	19.13047410995918	26.076731410613068	36.66624244954884	18.12655202987891
33	17.71877770777611	23.836970206285216	35.91716617355809	22.527085912380585
34	18.380556411585967	23.89492317287759	35.3841250942656	22.340395321270844
35	15.941798813804798	27.606647657532672	34.884951265867386	21.566602262795143
36	19.559599525017067	25.480162941444362	32.24299016272057	22.717247370818
37	18.14327529973043	27.301632043888592	35.2384538443011	19.31663881207988
38	20.191823267642786	28.738571117545654	30.594643715468816	20.474961899342745
39	19.48860451149646	25.904765711196493	31.750232179988664	22.856397597318388
40	18.7583550613134	27.324981515002033	34.27460450518579	19.642058918498776
41	17.98677073659167	25.841764210309314	31.790199743155817	24.381265309943192
42	18.950199364515747	25.67705577894151	34.87359206370409	20.499152792838654
43	20.157956016748514	23.548467506897033	32.36867763110149	23.924898845252958
44	19.652997409470842	23.515546856183033	33.453481437696354	23.37797429664977
45	19.127318776024932	23.13743267306218	33.17865185202326	24.55659669888964
46	21.000956066182077	25.16073796950054	32.60290858682059	21.23539737749679
47	16.52416828026938	23.98811070173575	38.46099639134975	21.026724626645112
48	18.96050678870096	24.417025761198015	33.529104274320524	23.0933631757805
49	18.838500543243324	22.533922469238124	37.129024759905384	21.498552227613168
50	18.07564597573969	22.274554019842842	35.530953300006	24.118846704411474
51	17.854982955937864	21.803883374650677	33.548036277926016	26.79309739148544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	1162.5
2	2001.0
3	28337.0
4	54673.0
5	36814.0
6	18955.0
7	18066.5
8	17178.0
9	16765.0
10	16352.0
11	15769.0
12	15186.0
13	14483.5
14	13781.0
15	12902.5
16	12024.0
17	11365.0
18	10706.0
19	9934.0
20	9162.0
21	8724.0
22	8286.0
23	8156.0
24	8026.0
25	8371.0
26	9317.0
27	9918.0
28	10912.5
29	11907.0
30	13171.5
31	14436.0
32	15793.0
33	17150.0
34	18658.0
35	20166.0
36	22044.0
37	23922.0
38	25718.0
39	27514.0
40	29760.5
41	32007.0
42	35911.0
43	39815.0
44	43337.0
45	46859.0
46	85322.0
47	123785.0
48	99395.5
49	75006.0
50	72369.0
51	69732.0
52	60862.5
53	51993.0
54	47156.0
55	42319.0
56	38575.5
57	34832.0
58	32026.5
59	29221.0
60	27251.5
61	25282.0
62	22292.5
63	19303.0
64	16904.0
65	14505.0
66	12121.0
67	9737.0
68	7975.5
69	6214.0
70	5156.0
71	4098.0
72	3278.5
73	2459.0
74	2004.0
75	1214.5
76	880.0
77	632.0
78	384.0
79	305.0
80	226.0
81	165.0
82	104.0
83	74.0
84	44.0
85	30.5
86	17.0
87	14.0
88	11.0
89	6.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	950771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.663501301437854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18460989236928	36.01704144163547
2	9.120163844393764	8.511575548443897
3	4.07999002069083	5.7115985892108005
4	2.5209839870098674	4.705517582349558
5	1.7823238492740872	4.158473563009254
6	1.2947900902081044	3.6251663435708834
7	0.9528937064859303	3.1125749698916705
8	0.7032074009759157	2.62512955764963
9	0.5169899596504567	2.171210548948145
>10	1.7668705897471177	11.970289543048136
>50	0.04416654008240329	1.4640199572645123
>100	0.026180439313529983	2.1575771518147464
>500	0.0020489039396179836	0.7006017375008624
>1k	0.003414839899363306	3.374892585089446
>5k	4.553119865817742E-4	1.205629889142879
>10k+	9.106239731635484E-4	8.488700991430084
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32809	3.4507783682926805	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	18508	1.9466306818361097	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	15873	1.669487184611226	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	12717	1.3375460547282152	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCT	5778	0.6077173157363865	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	5571	0.5859455115900674	TruSeq Adapter, Index 19 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTA	4447	0.46772566685353256	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCC	4382	0.4608891099959927	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTC	3284	0.3454038880024738	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT	3274	0.34435211002439076	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGAACACT	3171	0.3335187968501353	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCG	1728	0.18174723461275114	No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	1661	0.1747003221595947	TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGC	1637	0.17217605501219538	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	1481	0.15576831855409978	No Hit
CCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGC	1255	0.13199813624942283	TruSeq Adapter, Index 19 (95% over 23bp)
CGTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	1178	0.12389944581818334	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCT	1108	0.116536999971602	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGAACACTCGT	1086	0.11422308841981929	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGT	1067	0.1122247102614615	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTC	1010	0.1062295757863881	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATG	966	0.10160175268282268	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTG	960	0.10097068589597284	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18816308027905773	0.0
2	0.0	0.0	0.0	1.077441360748277	0.0
3	0.0	0.0	0.0	1.3748841729501635	0.0
4	0.0	0.0	0.0	2.3927949001389397	0.0
5	0.0	0.0	0.0	5.2015679906097265	0.0
6	0.0	0.0	0.0	6.058767042747412	0.0
7	0.0	0.0	0.0	7.135998047900073	0.0
8	0.0	0.0	0.0	8.422638048489068	0.0
9	0.0	0.0	0.0	9.022887740581066	0.0
10	0.0	0.0	0.0	11.705868184873118	0.0
11	0.0	0.0	0.0	13.07160188941396	0.0
12	0.0	0.0	0.0	15.463345011574816	0.0
13	0.0	0.0	0.0	15.988497756031684	0.0
14	0.0	0.0	0.0	16.25806845181437	0.0
15	0.0	0.0	0.0	17.001780660116896	0.0
16	0.0	0.0	0.0	17.50726515638361	0.0
17	0.0	0.0	0.0	18.06554890715009	0.0
18	0.0	0.0	0.0	18.63855754960974	0.0
19	0.0	0.0	0.0	19.389001136971995	0.0
20	0.0	0.0	0.0	19.857883759601417	0.0
21	0.0	0.0	0.0	20.330868316345367	0.0
22	0.0	0.0	0.0	20.90398213660282	0.0
23	0.0	0.0	0.0	21.39305889641144	0.0
24	0.0	0.0	0.0	21.779902836750384	0.0
25	0.0	0.0	0.0	22.129829370058616	0.0
26	0.0	0.0	0.0	22.48585621563973	0.0
27	0.0	0.0	0.0	22.85303190778852	0.0
28	0.0	0.0	0.0	23.19485975066551	0.0
29	0.0	0.0	0.0	23.571606622414862	0.0
30	0.0	0.0	0.0	23.996840458953837	0.0
31	0.0	0.0	0.0	24.375375353265927	0.0
32	0.0	0.0	0.0	24.74213033422349	0.0
33	0.0	0.0	0.0	25.133286564272574	0.0
34	0.0	0.0	0.0	25.517816593059738	0.0
35	0.0	0.0	0.0	25.908867645311016	0.0
36	0.0	0.0	0.0	26.28771807301653	0.0
37	0.0	0.0	0.0	26.672353279601502	0.0
38	0.0	0.0	0.0	27.118307142308716	0.0
39	0.0	0.0	0.0	27.683742983326162	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	40	6.8121153E-9	45.0	1
ACGTTAG	20	7.0326723E-4	45.0	1
ATCAGCG	20	7.0326723E-4	45.0	1
TAGTGCG	40	6.8121153E-9	45.0	1
CGTTTTT	18625	0.0	44.166443	1
TACGGCT	1410	0.0	40.05319	7
GCGTAAG	45	1.927765E-8	40.0	1
TACGAAT	85	0.0	39.705883	12
CGAATAT	85	0.0	39.705883	14
CTAGCGG	125	0.0	39.6	2
CGTTTTC	165	0.0	39.545452	1
ACGTACG	40	3.4573895E-7	39.375	1
GCTATCG	35	6.247148E-6	38.57143	1
CTAAGCG	35	6.247148E-6	38.57143	1
AGACGTT	35	6.247148E-6	38.57143	11
GTATGCG	35	6.247148E-6	38.57143	1
CGAATTG	35	6.247148E-6	38.57143	32
GTTTTTT	21730	0.0	38.56995	2
TGATACC	1880	0.0	38.29787	4
ACGGCTG	1490	0.0	38.204697	8
>>END_MODULE