FastQCFastQC Report
Sat 18 Jun 2016
SRR3552287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103785
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251702.280335391403217No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC68370.6194141069139371No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG50510.4576072332927155No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC41010.37153974732398065No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC25570.2316574332863737No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT18350.16624614395013523No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT13070.1184107412222489No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.1150586391371508No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC12110.10971339527172412No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC11850.10735786407679031No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.033166E-445.021
TGTTACG207.033166E-445.01
TCGAACG207.033166E-445.01
CGTTTTT163300.044.2284161
CACGACC5800.040.73275827
ACACGAC5950.039.70588326
TCAAGCG5750.039.5217417
TAGCACG403.4580626E-739.3751
CGCACGG800.039.3752
ACGACCA5900.039.27966328
CGACCAA5850.039.2307729
CATACGA700.038.57142618
ACATACG700.038.57142617
TAAGGGA9200.038.396744
GCGAGAC6100.037.99180221
CACGTGA5450.037.9816543
GTTTTTT198250.037.3846172
AGACACG6200.037.37903224
ACACGTG5600.037.3660742
CGAGACA6150.037.31707422