FastQCFastQC Report
Sat 18 Jun 2016
SRR3552286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848668
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180162.1228560520721884No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC45260.5333063105949559No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG36080.42513680261303594No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC32880.3874306560398177No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC21730.2560483015737603No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT14490.17073814495185397No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT12740.1501175960446252No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC11520.13574212766358576No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC10660.12560860077203334No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT9250.10899432993820904No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG8880.10463455674068069No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG253.889941E-545.01
TACCGCG207.0322433E-445.01
CGTTTTT139150.044.1430131
CTAACGG700.041.7857172
TACGAAT556.002665E-1140.90909212
CGATGTA403.4567893E-739.37510
ACAACGA3450.039.13043613
TCGATAG356.246357E-638.571431
CTTGCGG700.038.571432
TATCGAG356.246357E-638.571431
GTTTTTT161650.038.4441682
CGCGAGG1700.038.382352
CACAACG3600.038.12512
GCGAGAC3750.037.821
TAGCCGT301.1396704E-437.49999644
CGAATAT601.546141E-1037.49999614
CGACCAA3850.036.81818429
TACGGCT4400.036.818187
CGTAAGG1100.036.818182
GCAAGCG1050.036.428571