##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	848668
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.95867052840451	28.0	16.0	31.0	16.0	33.0
2	27.26637389414942	30.0	25.0	33.0	16.0	33.0
3	27.363593301503062	31.0	25.0	33.0	16.0	34.0
4	30.210856306588678	32.0	28.0	35.0	19.0	37.0
5	32.39669694156019	33.0	32.0	35.0	28.0	37.0
6	31.391016274915515	35.0	31.0	35.0	17.0	37.0
7	33.47056210438004	35.0	32.0	35.0	29.0	37.0
8	34.19977659108155	35.0	35.0	37.0	32.0	37.0
9	35.577406005646495	37.0	35.0	39.0	30.0	39.0
10	35.690454924658404	37.0	35.0	39.0	31.0	39.0
11	36.0542508966993	37.0	35.0	39.0	31.0	39.0
12	35.829568217489054	37.0	35.0	39.0	31.0	39.0
13	36.109872176163115	37.0	35.0	39.0	32.0	39.0
14	36.54285185726338	38.0	35.0	40.0	31.0	41.0
15	36.878919671767996	38.0	35.0	40.0	32.0	41.0
16	36.58042721064067	38.0	34.0	40.0	30.0	41.0
17	36.437709445861046	38.0	34.0	40.0	31.0	41.0
18	36.08418604212719	38.0	34.0	39.0	30.0	40.0
19	35.37748683819821	37.0	34.0	39.0	27.0	41.0
20	35.80355686793894	37.0	34.0	39.0	31.0	40.0
21	35.56203721596667	37.0	34.0	39.0	30.0	40.0
22	36.141677310797625	38.0	34.0	40.0	31.0	40.0
23	36.67334693896789	38.0	35.0	40.0	32.0	41.0
24	36.68162697309195	38.0	35.0	40.0	32.0	41.0
25	34.66129157691818	36.0	33.0	39.0	25.0	41.0
26	35.378358792837716	37.0	34.0	39.0	29.0	41.0
27	36.145675340651465	38.0	35.0	40.0	31.0	41.0
28	36.244230959574296	38.0	35.0	40.0	31.0	41.0
29	36.313867142392546	38.0	35.0	40.0	31.0	41.0
30	35.47134568523852	38.0	34.0	40.0	29.0	41.0
31	35.45586849038729	37.0	34.0	40.0	29.0	41.0
32	35.15357124340731	38.0	34.0	40.0	26.0	41.0
33	34.920514264706576	38.0	35.0	40.0	23.0	41.0
34	34.57088284228933	38.0	34.0	40.0	18.0	41.0
35	34.30838678965155	38.0	34.0	40.0	17.0	41.0
36	34.0194127738998	38.0	34.0	40.0	15.0	41.0
37	34.072616146714616	38.0	34.0	40.0	15.0	41.0
38	33.752494497259235	38.0	33.0	40.0	15.0	41.0
39	33.71487908110121	38.0	33.0	40.0	15.0	41.0
40	33.531001522385665	38.0	33.0	40.0	15.0	41.0
41	33.405998576592964	38.0	33.0	40.0	14.0	41.0
42	33.58835257132353	38.0	33.0	40.0	12.0	41.0
43	33.60043621298317	38.0	33.0	40.0	12.0	41.0
44	33.60113377669477	38.0	33.0	40.0	12.0	41.0
45	33.596643210301316	38.0	33.0	40.0	12.0	41.0
46	33.43926128945595	38.0	33.0	40.0	10.0	41.0
47	33.45124477416375	38.0	33.0	40.0	10.0	41.0
48	33.419738932067666	38.0	33.0	40.0	10.0	41.0
49	33.44046670782921	37.0	33.0	40.0	10.0	41.0
50	33.39531477562486	37.0	33.0	40.0	10.0	41.0
51	32.400037470483156	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	50.0
10	55.0
11	46.0
12	49.0
13	51.0
14	89.0
15	134.0
16	232.0
17	406.0
18	783.0
19	1414.0
20	2194.0
21	3590.0
22	5247.0
23	8178.0
24	12839.0
25	19928.0
26	25655.0
27	26555.0
28	23761.0
29	21856.0
30	23029.0
31	27843.0
32	37115.0
33	50296.0
34	64952.0
35	91966.0
36	124104.0
37	152533.0
38	107199.0
39	16502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.511884506073045	17.429901916886227	20.402206752228196	26.65600682481253
2	42.83323985351162	18.80488011802024	21.406839894988384	16.95504013347976
3	27.894535908034708	18.8318635791617	37.85355403997794	15.420046472825652
4	23.95070863989216	21.888300254045163	37.192282494450126	16.968708611612552
5	22.409705562127947	25.069402876036328	35.74872623923607	16.77216532259965
6	22.204914053552155	28.295517210499277	35.28588329004982	14.213685445898749
7	68.82950694500087	4.145672984017308	23.29285421389754	3.731965857084278
8	69.93794982254545	4.407966366117257	21.663713018518433	3.990370792818864
9	63.961643422398396	5.862480970179151	23.47490420282136	6.700971404601093
10	30.230667351661662	28.017552211229834	29.423284488162626	12.328495948945879
11	23.045171963594715	21.469172868542234	39.700330400109344	15.785324767753703
12	20.171020941051154	20.366975071523846	41.775464610424805	17.686539377000194
13	19.674831618489208	20.96508882154152	43.07997945015012	16.280100109819152
14	18.128408282155096	22.764850330164446	41.08355682080625	18.023184566874207
15	17.679351642809674	22.167325738686976	42.84466952919163	17.308653089311722
16	20.583785414319852	22.844151069676247	39.69043253663388	16.881630979370023
17	20.257627246461514	21.93684691775818	39.885090518318115	17.92043531746219
18	21.023062021897847	21.43453034637809	39.72024395876833	17.82216367295574
19	19.722435628537895	23.614299113434228	38.385917696908564	18.277347561119306
20	21.584530110714674	23.107151442024442	39.01160406660791	16.296714380652976
21	21.044153897637237	23.958367700914845	38.63631007649634	16.36116832495157
22	20.48339279906866	20.83252814999505	39.30029175130911	19.38378729962718
23	19.084023434370096	23.76394538264669	39.477510640203235	17.67452054277998
24	19.409121116856063	21.88712193696475	40.07515306338875	18.628603882790443
25	19.131509612710744	23.256326384404737	38.48795995607234	19.12420404681218
26	18.939561760311452	22.89576135779834	39.068281118175776	19.096395763714433
27	19.57231803249327	22.17027153138801	38.98909821037202	19.2683122257467
28	18.141016274915515	23.41174640731122	39.84066796438655	18.606569353386718
29	19.18818666427861	21.768465406967152	39.691257358590164	19.35209057016407
30	20.00299292538425	21.377264136270014	40.35865615293613	18.261086785409606
31	21.103423246782015	21.901026078513624	38.073074512058895	18.922476162645463
32	20.39419419608139	22.539202609265345	38.10005797320036	18.96654522145291
33	19.182295078876546	22.757898259390007	36.44416898009587	21.61563768163758
34	19.570668388580696	23.934919191014625	36.44381548497174	20.05059693543294
35	18.637912587725708	24.471053462602573	35.45520745450518	21.435826495166545
36	20.32043154684753	25.725725489826413	33.75018263914746	20.203660324178596
37	20.432842996318936	25.041123266106418	34.23128950308012	20.294744234494523
38	20.39242672046077	25.90671499337786	32.7208048376986	20.98005344846277
39	21.36795543133475	23.90840705670533	32.90686110469583	21.816776407264086
40	21.60302968887716	23.631031215976094	33.396098356483336	21.369840738663413
41	19.535554539584385	25.1985464280496	33.31396965597854	21.95192937638747
42	20.95236299707306	25.043126405143116	33.0074893833631	20.997021214420716
43	21.36159251910052	24.187314709639104	33.47504560087101	20.976047170389364
44	21.52478943473773	24.784250142576365	32.38993340151862	21.301027021167286
45	21.09647117600758	23.654597557584356	32.57410436118718	22.674826905220886
46	21.378913780182593	24.417086540319655	32.273044347141635	21.930955332356117
47	19.743056177445126	24.26531930036245	34.71192504018062	21.27969948201181
48	20.50896227971362	24.15738545579661	33.46349809348296	21.8701541710068
49	20.48080050149175	23.61076416219299	34.256623320309004	21.651812016006257
50	19.40558616561482	23.368737833876143	34.9147134097197	22.310962590789334
51	19.676363430693748	22.84803951604161	33.379012758817346	24.096584294447297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	507.0
2	849.0
3	17469.5
4	34090.0
5	24190.5
6	14291.0
7	13789.0
8	13287.0
9	13177.0
10	13067.0
11	12622.5
12	12178.0
13	11698.0
14	11218.0
15	10628.0
16	10038.0
17	9374.5
18	8711.0
19	8113.0
20	7515.0
21	7075.0
22	6635.0
23	6627.0
24	6619.0
25	6851.0
26	7726.0
27	8369.0
28	9734.0
29	11099.0
30	12941.5
31	14784.0
32	16487.0
33	18190.0
34	19762.5
35	21335.0
36	23783.5
37	26232.0
38	28356.0
39	30480.0
40	32537.0
41	34594.0
42	36485.5
43	38377.0
44	41851.0
45	45325.0
46	57796.0
47	70267.0
48	67043.5
49	63820.0
50	63549.5
51	63279.0
52	58084.5
53	52890.0
54	48987.5
55	45085.0
56	41037.5
57	36990.0
58	33598.0
59	30206.0
60	27396.0
61	24586.0
62	22644.5
63	20703.0
64	18197.0
65	15691.0
66	12783.0
67	9875.0
68	8404.5
69	6934.0
70	5716.0
71	4498.0
72	3905.5
73	3313.0
74	2936.5
75	2192.0
76	1824.0
77	1351.0
78	878.0
79	660.0
80	442.0
81	322.0
82	202.0
83	131.5
84	61.0
85	38.0
86	15.0
87	14.0
88	13.0
89	7.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	848668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.78796513747497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49608182168458	30.793315533588288
2	7.727127915481136	6.303476480589085
3	3.282872139692493	4.017050231536957
4	2.1723142159955167	3.5441710603866556
5	1.7081342012886847	3.4835659126145764
6	1.3600130281164033	3.3283298386394176
7	1.1783209078886767	3.3642918478204837
8	1.0326730214675506	3.3696504958423517
9	0.950738787601798	3.490083047119261
>10	5.010239827039427	30.093525080109018
>50	0.05578311678814296	1.5672018968133854
>100	0.020736047771155865	1.5780371086101974
>500	0.002336456086792429	0.7130769330352476
>1k	0.002336456086792429	2.2080845357068744
>5k	0.0	0.0
>10k+	2.920570108490536E-4	2.146139997588209
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18016	2.1228560520721884	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	4526	0.5333063105949559	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG	3608	0.42513680261303594	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC	3288	0.3874306560398177	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	2173	0.2560483015737603	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT	1449	0.17073814495185397	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT	1274	0.1501175960446252	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	1152	0.13574212766358576	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	1066	0.12560860077203334	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	925	0.10899432993820904	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	888	0.10463455674068069	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0999212884190284	0.0
2	0.0	0.0	0.0	0.5769040425702395	0.0
3	0.0	0.0	0.0	0.811153478156358	0.0
4	0.0	0.0	0.0	1.2407678856749635	0.0
5	0.0	0.0	0.0	2.435463573505776	0.0
6	0.0	0.0	0.0	3.2667662737371974	0.0
7	0.0	0.0	0.0	3.9693967487875117	0.0
8	0.0	0.0	0.0	4.896496627656516	0.0
9	0.0	0.0	0.0	5.420376401608167	0.0
10	0.0	0.0	0.0	6.581607884355249	0.0
11	0.0	0.0	0.0	7.323947645015483	0.0
12	0.0	0.0	0.0	8.272728558164088	0.0
13	0.0	0.0	0.0	8.585925238137882	0.0
14	0.0	0.0	0.0	8.74169875616849	0.0
15	0.0	0.0	0.0	8.988438352806988	0.0
16	0.0	0.0	0.0	9.286081247319329	0.0
17	0.0	0.0	0.0	9.68553073757936	0.0
18	0.0	0.0	0.0	10.116323462178379	0.0
19	0.0	0.0	0.0	10.473117874127457	0.0
20	0.0	0.0	0.0	10.74389513920638	0.0
21	0.0	0.0	0.0	11.035764280024697	0.0
22	0.0	0.0	0.0	11.368992350365515	0.0
23	0.0	0.0	0.0	11.68136420838302	0.0
24	0.0	0.0	0.0	11.945425066103589	0.0
25	0.0	0.0	0.0	12.175314728492179	0.0
26	0.0	0.0	0.0	12.403201251844067	0.0
27	0.0	0.0	0.0	12.645934570409159	0.0
28	0.0	0.0	0.0	12.893381157295904	0.0
29	0.0	0.0	0.0	13.149547290577706	0.0
30	0.0	0.0	0.0	13.454849246112731	0.0
31	0.0	0.0	0.0	13.73399256246259	0.0
32	0.0	0.0	0.0	14.03340293259555	0.0
33	0.0	0.0	0.0	14.30830430745592	0.0
34	0.0	0.0	0.0	14.572836492008653	0.0
35	0.0	0.0	0.0	14.865294791367178	0.0
36	0.0	0.0	0.0	15.141963641848166	0.0
37	0.0	0.0	0.0	15.416511521584413	0.0
38	0.0	0.0	0.0	15.725701923484802	0.0
39	0.0	0.0	0.0	16.05091743767881	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	25	3.889941E-5	45.0	1
TACCGCG	20	7.0322433E-4	45.0	1
CGTTTTT	13915	0.0	44.143013	1
CTAACGG	70	0.0	41.785717	2
TACGAAT	55	6.002665E-11	40.909092	12
CGATGTA	40	3.4567893E-7	39.375	10
ACAACGA	345	0.0	39.130436	13
TCGATAG	35	6.246357E-6	38.57143	1
CTTGCGG	70	0.0	38.57143	2
TATCGAG	35	6.246357E-6	38.57143	1
GTTTTTT	16165	0.0	38.444168	2
CGCGAGG	170	0.0	38.38235	2
CACAACG	360	0.0	38.125	12
GCGAGAC	375	0.0	37.8	21
TAGCCGT	30	1.1396704E-4	37.499996	44
CGAATAT	60	1.546141E-10	37.499996	14
CGACCAA	385	0.0	36.818184	29
TACGGCT	440	0.0	36.81818	7
CGTAAGG	110	0.0	36.81818	2
GCAAGCG	105	0.0	36.42857	1
>>END_MODULE