FastQCFastQC Report
Sat 18 Jun 2016
SRR3552282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416732
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC84562.0291218336964767No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC82131.9708109768388316No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG82001.9676914659781348No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63461.5228012247679563No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC28080.6738143459105612TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCACTGT18080.43385197201078873No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT17720.42521332655039684No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTA15370.36882216868395035No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCT14530.34866532927636945No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTC10830.25987925093345365No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT5980.14349749959206398No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATG4570.10966280487219604No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC4530.10870295537659695TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT4210.10102415941180423TruSeq Adapter, Index 13 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT302.1621581E-645.00000414
CGTGGCA207.0281053E-445.015
GCGAACC207.0281053E-445.033
CCGGACT207.0281053E-445.038
CCGTCGA207.0281053E-445.041
CGTTTTT46050.043.729641
TGATACC10050.042.0895544
CCGATGA5200.041.97115318
ATACCTG10200.041.6911776
CGATGAA5250.041.5714319
GATACCT10250.041.268295
GCGAGAC2250.041.021
TACGGCT10000.040.7257
ACGGCTG10050.040.522398
CAACGAG1000.040.514
AACGAGC1000.040.515
CGGCTGT10250.039.9512189
CGAGGGA1300.039.807694
ACACGAC2400.039.37500426
CGGGATC403.4511504E-739.3756
Produced by FastQC (version 0.11.5)