##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	416732
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.826053674783793	28.0	16.0	31.0	16.0	33.0
2	26.96686599541192	30.0	25.0	31.0	16.0	33.0
3	27.119594847527907	30.0	25.0	33.0	16.0	34.0
4	30.904703742453183	33.0	28.0	35.0	19.0	37.0
5	32.51443613641381	35.0	32.0	35.0	28.0	37.0
6	31.45651881784936	35.0	31.0	35.0	17.0	37.0
7	33.13282877244848	35.0	32.0	35.0	28.0	37.0
8	33.363802155821965	35.0	33.0	36.0	28.0	37.0
9	34.90288722728276	37.0	34.0	39.0	28.0	39.0
10	35.11192325043433	37.0	34.0	39.0	30.0	39.0
11	35.504300125740286	37.0	34.0	39.0	30.0	39.0
12	35.337415893187945	37.0	34.0	39.0	30.0	39.0
13	35.43848084620331	37.0	34.0	39.0	30.0	39.0
14	35.95018861042588	38.0	34.0	40.0	30.0	41.0
15	36.21011825345786	38.0	34.0	40.0	30.0	41.0
16	35.935349337223926	38.0	34.0	40.0	30.0	41.0
17	35.702940978854514	37.0	34.0	40.0	29.0	41.0
18	35.487529155428426	37.0	34.0	39.0	27.0	40.0
19	34.850181891479416	37.0	33.0	39.0	27.0	40.0
20	35.016051083190156	36.0	33.0	39.0	28.0	40.0
21	35.18502058877168	37.0	34.0	39.0	29.0	40.0
22	35.76852509526506	37.0	34.0	39.0	30.0	40.0
23	36.2276451052475	38.0	35.0	40.0	31.0	41.0
24	36.23792989259284	38.0	35.0	40.0	31.0	41.0
25	34.40783765105631	36.0	33.0	39.0	25.0	40.0
26	35.032906040332875	37.0	33.0	39.0	27.0	40.0
27	35.63559553861955	37.0	34.0	40.0	30.0	41.0
28	35.62663294395439	38.0	34.0	40.0	30.0	41.0
29	35.79510812704568	38.0	35.0	40.0	30.0	41.0
30	34.99171409922924	37.0	34.0	40.0	26.0	41.0
31	35.150972327539044	37.0	34.0	40.0	27.0	41.0
32	34.88012439649463	37.0	34.0	40.0	25.0	41.0
33	34.776376664139065	38.0	34.0	40.0	24.0	41.0
34	34.68415192497817	38.0	34.0	40.0	22.0	41.0
35	34.55341322480635	38.0	34.0	40.0	21.0	41.0
36	34.28418503978576	38.0	34.0	40.0	20.0	41.0
37	34.364402061756714	38.0	34.0	40.0	19.0	41.0
38	34.00969208028181	38.0	33.0	40.0	18.0	41.0
39	34.02830116237774	38.0	34.0	40.0	18.0	41.0
40	33.71751149419771	37.0	33.0	40.0	18.0	41.0
41	33.64349030072085	37.0	33.0	40.0	18.0	41.0
42	33.89991889271762	38.0	33.0	40.0	18.0	41.0
43	33.93965666183542	38.0	33.0	40.0	18.0	41.0
44	34.10557144639721	38.0	34.0	40.0	18.0	41.0
45	34.09909726154939	38.0	34.0	40.0	18.0	41.0
46	33.925573270111244	37.0	33.0	40.0	18.0	41.0
47	33.819593887678415	37.0	33.0	40.0	19.0	41.0
48	33.84986754076961	37.0	33.0	40.0	18.0	41.0
49	33.92407350527437	37.0	34.0	40.0	18.0	41.0
50	33.77127746369369	37.0	33.0	40.0	18.0	41.0
51	32.68012775596787	35.0	31.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	23.0
10	29.0
11	29.0
12	40.0
13	34.0
14	48.0
15	91.0
16	126.0
17	267.0
18	426.0
19	849.0
20	1520.0
21	2211.0
22	3205.0
23	4386.0
24	5988.0
25	8078.0
26	10095.0
27	10788.0
28	10722.0
29	11298.0
30	13224.0
31	16937.0
32	22473.0
33	30063.0
34	36817.0
35	46660.0
36	60560.0
37	68873.0
38	44440.0
39	6419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67379514892065	16.16050603265408	18.832007141280247	20.333691677145023
2	37.63737845905762	26.56791415106111	19.33592812646977	16.458779263411497
3	27.62902776844591	26.387942370636285	31.064809037942855	14.918220822974956
4	24.556069608285423	20.620686676329154	38.21616770490387	16.607076010481556
5	28.129589280400836	23.5249512876381	29.908190395745947	18.43726903621512
6	21.850733804939384	33.79270130443546	30.940028603514968	13.416536287110182
7	65.63186892295289	4.973700123820585	25.351544877763168	4.042886075463367
8	64.96885288386781	11.041388710250233	18.62683931159594	5.362919094286016
9	59.78038643540693	5.475461447645009	19.15523645892324	15.58891565802482
10	36.43852643905436	20.389362947889772	27.02408262384458	16.148027989211293
11	30.310127372028067	21.13780559208316	31.136077862991087	17.41598917289769
12	24.015194417515335	19.041254331320847	35.662008197114694	21.281543054049127
13	23.658130405152473	20.314494687233044	39.455093441348396	16.57228146626609
14	20.33537141376232	25.744603246210996	34.164642983980116	19.75538235604657
15	18.788813913978288	21.01134542103798	41.18810170565255	19.011738959331176
16	21.293541172744114	22.398087979804767	33.1184070337771	23.189963813674016
17	20.692195463751283	23.46664043078045	35.85109854774771	19.99006555772055
18	22.17156349884338	21.08621368169471	34.901087509478515	21.841135309983393
19	20.37808471631648	23.35841740015166	33.40516207058733	22.85833581294453
20	24.33794381041053	22.661806628720615	36.78095274660933	16.219296814259522
21	23.00759240951019	26.752445216590036	33.069694671875446	17.170267702024322
22	20.662920054135512	20.37592505495138	36.262394056611924	22.698760834301183
23	22.02854592399912	24.74539992129234	34.8123014311356	18.413752723572944
24	22.682443392876	22.050142537650096	32.917558526822994	22.34985554265091
25	19.64068034132248	27.040400065269765	32.12640258007544	21.19251701333231
26	19.47318660434044	21.54838121382567	36.57962431490742	22.398807866926465
27	23.03566800725646	22.86289509804863	32.533618728583356	21.567818166111554
28	17.578923624775637	24.721163721528463	37.30863000681493	20.391282646880967
29	22.09957478667345	20.97031185510112	35.60033786702245	21.32977549120298
30	22.402167340161064	24.356900837948608	34.921484311259995	18.319447510630333
31	22.738114663620745	20.985669447030705	33.336532831652	22.939683057696552
32	23.768753059520268	24.249637656815413	31.72062620581093	20.260983077853393
33	22.324659493391437	24.00943532054174	29.718860082739027	23.9470451033278
34	21.337694249541673	23.048625975447052	33.3355729821564	22.278106792854878
35	22.976397300903216	24.13445571734352	31.522417284969716	21.366729696783544
36	23.039027480491058	26.179175105343482	31.701429215898948	19.080368198266513
37	23.271551020799937	24.106380119597247	30.913392780012096	21.70867607959072
38	21.250347945442154	27.347071979113675	29.357476747645965	22.045103327798202
39	23.38577311077623	23.68260656729025	27.88050833629287	25.051111985640652
40	21.503268287532514	24.777554879394913	32.34668803931544	21.37248879375714
41	22.073418887918375	25.243561809508268	28.349874739640825	24.33314456293253
42	21.451436414770164	22.97615733852932	33.848372575180214	21.724033671520306
43	25.155975543034852	22.97759711277272	28.74341303283645	23.12301431135598
44	22.70907921637887	22.726836432047453	30.512175690851674	24.051908660722
45	21.241229375233964	21.546461514834476	31.17975101504084	26.032558094890724
46	25.20084850695411	24.199485520670358	29.117754336120093	21.481911636255436
47	19.48494476066153	23.750275956729986	36.022191720338256	20.742587562270238
48	21.88960770951115	23.8397819221946	30.354280448825627	23.916329919468627
49	21.080934509468914	21.86753117111237	34.76334910686004	22.28818521255867
50	21.56133918201626	22.29082479867157	32.196951518001974	23.950884501310192
51	20.81097683883167	21.497029265811122	30.952026722209958	26.73996717314725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	282.5
2	492.0
3	6480.0
4	12468.0
5	8648.0
6	4828.0
7	4641.5
8	4455.0
9	4345.0
10	4235.0
11	4099.5
12	3964.0
13	3786.5
14	3609.0
15	3527.0
16	3445.0
17	3154.0
18	2863.0
19	2701.5
20	2540.0
21	2419.5
22	2299.0
23	2303.5
24	2308.0
25	2356.0
26	2599.5
27	2795.0
28	3126.5
29	3458.0
30	3960.5
31	4463.0
32	5062.0
33	5661.0
34	6417.0
35	7173.0
36	8244.0
37	9315.0
38	10219.5
39	11124.0
40	12330.5
41	13537.0
42	14650.5
43	15764.0
44	18236.5
45	20709.0
46	38639.0
47	56569.0
48	44883.5
49	33198.0
50	33529.5
51	33861.0
52	30458.5
53	27056.0
54	25511.0
55	23966.0
56	22498.5
57	21031.0
58	19625.0
59	18219.0
60	17100.0
61	15981.0
62	14762.5
63	13544.0
64	11810.0
65	10076.0
66	8331.0
67	6586.0
68	5460.5
69	4335.0
70	3849.5
71	3364.0
72	2675.0
73	1986.0
74	1677.0
75	1118.0
76	868.0
77	603.5
78	339.0
79	285.0
80	231.0
81	178.0
82	125.0
83	78.0
84	31.0
85	20.0
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	416732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.09023135331974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.75128505246906	29.967963117114355
2	7.77804118770591	6.236469413815596
3	3.5822627966518215	4.3084123285848515
4	2.4718512861247146	3.9638835972696302
5	1.9307299815843801	3.870170582125428
6	1.6412581521378127	3.9479051417836177
7	1.3646085215182844	3.8295229934065733
8	1.1701790899849458	3.7530200353850915
9	1.0377543398719604	3.744343041604065
>10	4.209703699215848	23.748677830703187
>50	0.03993697981094961	1.1008656917680057
>100	0.015732749622495303	1.2735885592291825
>500	6.051057547113577E-4	0.14506780160362878
>1k	0.003630634528268146	2.5377161748755195
>5k	0.002420423018845431	7.572393690731226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	8456	2.0291218336964767	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	8213	1.9708109768388316	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	8200	1.9676914659781348	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6346	1.5228012247679563	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	2808	0.6738143459105612	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCACTGT	1808	0.43385197201078873	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCT	1772	0.42521332655039684	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTA	1537	0.36882216868395035	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCT	1453	0.34866532927636945	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTC	1083	0.25987925093345365	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT	598	0.14349749959206398	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATG	457	0.10966280487219604	No Hit
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	453	0.10870295537659695	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	421	0.10102415941180423	TruSeq Adapter, Index 13 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10414367027250127	0.0
2	0.0	0.0	0.0	1.0124012554831403	0.0
3	0.0	0.0	0.0	1.2286073543668352	0.0
4	0.0	0.0	0.0	1.9098605338682895	0.0
5	0.0	0.0	0.0	4.590480212702648	0.0
6	0.0	0.0	0.0	5.1627904744536055	0.0
7	0.0	0.0	0.0	5.910993156273096	0.0
8	0.0	0.0	0.0	6.700229404029448	0.0
9	0.0	0.0	0.0	6.98314504285728	0.0
10	0.0	0.0	0.0	9.518347523108377	0.0
11	0.0	0.0	0.0	10.188562433410441	0.0
12	0.0	0.0	0.0	12.778716297284586	0.0
13	0.0	0.0	0.0	13.158576735167925	0.0
14	0.0	0.0	0.0	13.353426182774541	0.0
15	0.0	0.0	0.0	13.906539454613517	0.0
16	0.0	0.0	0.0	14.325753721816419	0.0
17	0.0	0.0	0.0	14.787681291573481	0.0
18	0.0	0.0	0.0	15.216734016106274	0.0
19	0.0	0.0	0.0	15.961337262317269	0.0
20	0.0	0.0	0.0	16.326080070644924	0.0
21	0.0	0.0	0.0	16.645709952679418	0.0
22	0.0	0.0	0.0	17.03588877264045	0.0
23	0.0	0.0	0.0	17.356478504170546	0.0
24	0.0	0.0	0.0	17.6168376798518	0.0
25	0.0	0.0	0.0	17.85751994087327	0.0
26	0.0	0.0	0.0	18.104921148363935	0.0
27	0.0	0.0	0.0	18.383037539713772	0.0
28	0.0	0.0	0.0	18.622040064117947	0.0
29	0.0	0.0	0.0	18.86056266377432	0.0
30	0.0	0.0	0.0	19.151637023314745	0.0
31	0.0	0.0	0.0	19.401197892170508	0.0
32	0.0	0.0	0.0	19.68531334286784	0.0
33	0.0	0.0	0.0	19.9370338730887	0.0
34	0.0	0.0	0.0	20.193553650787557	0.0
35	0.0	0.0	0.0	20.451273240355913	0.0
36	0.0	0.0	0.0	20.69387520036858	0.0
37	0.0	0.0	0.0	20.955434187919334	0.0
38	0.0	0.0	0.0	21.23283069214747	0.0
39	0.0	0.0	0.0	21.532303734774388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	30	2.1621581E-6	45.000004	14
CGTGGCA	20	7.0281053E-4	45.0	15
GCGAACC	20	7.0281053E-4	45.0	33
CCGGACT	20	7.0281053E-4	45.0	38
CCGTCGA	20	7.0281053E-4	45.0	41
CGTTTTT	4605	0.0	43.72964	1
TGATACC	1005	0.0	42.089554	4
CCGATGA	520	0.0	41.971153	18
ATACCTG	1020	0.0	41.691177	6
CGATGAA	525	0.0	41.57143	19
GATACCT	1025	0.0	41.26829	5
GCGAGAC	225	0.0	41.0	21
TACGGCT	1000	0.0	40.725	7
ACGGCTG	1005	0.0	40.52239	8
CAACGAG	100	0.0	40.5	14
AACGAGC	100	0.0	40.5	15
CGGCTGT	1025	0.0	39.951218	9
CGAGGGA	130	0.0	39.80769	4
ACACGAC	240	0.0	39.375004	26
CGGGATC	40	3.4511504E-7	39.375	6
>>END_MODULE