FastQCFastQC Report
Sat 18 Jun 2016
SRR3552281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646735
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113801.7596078764872785No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC46860.7245626106519673No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG45620.7053893789573783No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC43190.6678160297494338No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC18350.28373290451266747No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT8140.1258629887048018No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT7790.12045118943616784No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG253.8889062E-545.0000041
CTTCGCA207.030996E-445.030
AACGACC207.030996E-445.040
CGTTTTT62850.043.317421
TTTACGG900.042.5000042
GACCTAT556.002665E-1140.90909233
AAACACG2150.039.7674440
CACGACC2250.039.00000427
GTTTACG356.244052E-638.5714261
GAAACGT356.244052E-638.57142631
CGAAAGG1000.038.2500042
ATTGCGG659.094947E-1238.076922
AACACGT2150.037.6744241
CGTCCCG301.1393698E-437.50000441
ATCGAGT301.1393698E-437.50000423
TCAAACG301.1393698E-437.5000041
CACGTGA2300.037.17391243
TACGAAT552.744855E-936.8181812
CGAATAT552.744855E-936.8181814
GTACGAG552.744855E-936.818181