##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646735
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.995896309926014	30.0	26.0	31.0	16.0	33.0
2	28.690418795951974	31.0	28.0	33.0	16.0	34.0
3	28.280100814089234	31.0	27.0	33.0	16.0	34.0
4	32.320319760025356	35.0	32.0	35.0	25.0	37.0
5	33.406282325836706	35.0	33.0	35.0	28.0	37.0
6	33.27435812195103	35.0	33.0	35.0	28.0	37.0
7	34.212895544542974	35.0	33.0	36.0	31.0	37.0
8	34.40713584389278	35.0	35.0	37.0	31.0	37.0
9	35.70937864813254	37.0	35.0	39.0	30.0	39.0
10	35.5906484108638	37.0	35.0	39.0	30.0	39.0
11	35.76749518736422	37.0	35.0	39.0	31.0	39.0
12	35.63853974193449	37.0	35.0	39.0	30.0	39.0
13	35.77168391999815	37.0	35.0	39.0	31.0	39.0
14	36.796640045768356	38.0	35.0	40.0	31.0	41.0
15	37.047452202215744	39.0	35.0	40.0	32.0	41.0
16	36.78493509706448	38.0	35.0	40.0	31.0	41.0
17	35.84317842702189	38.0	34.0	40.0	29.0	41.0
18	35.60432480072982	38.0	34.0	39.0	29.0	41.0
19	35.10356637571803	37.0	33.0	39.0	27.0	40.0
20	35.61781873564907	37.0	34.0	39.0	30.0	40.0
21	35.869394728907515	37.0	34.0	39.0	30.0	40.0
22	36.27889320973814	38.0	35.0	40.0	31.0	41.0
23	36.50819887589198	38.0	35.0	40.0	31.0	41.0
24	36.454087068119094	38.0	35.0	40.0	31.0	41.0
25	35.76031295662057	38.0	34.0	40.0	30.0	41.0
26	35.92359467169706	38.0	35.0	40.0	30.0	41.0
27	36.23845160691783	38.0	35.0	40.0	31.0	41.0
28	36.1882795890125	38.0	35.0	40.0	31.0	41.0
29	36.25920199154213	38.0	35.0	40.0	31.0	41.0
30	35.69819013970173	38.0	35.0	40.0	29.0	41.0
31	35.749316180506696	38.0	35.0	40.0	30.0	41.0
32	35.584684608069765	38.0	35.0	40.0	29.0	41.0
33	35.38496911408846	38.0	35.0	40.0	27.0	41.0
34	35.096597524488395	38.0	34.0	40.0	25.0	41.0
35	34.84917779306826	38.0	34.0	40.0	23.0	41.0
36	34.594224837066186	38.0	34.0	40.0	22.0	41.0
37	34.56304050345196	38.0	34.0	40.0	22.0	41.0
38	34.42751049502501	38.0	34.0	40.0	22.0	41.0
39	34.27684909584296	38.0	34.0	40.0	21.0	41.0
40	34.191177220963766	38.0	34.0	40.0	21.0	41.0
41	34.07512505121881	37.0	33.0	40.0	20.0	41.0
42	33.986283408196556	37.0	33.0	40.0	20.0	41.0
43	33.87609299017372	37.0	33.0	40.0	20.0	41.0
44	33.762194716537685	37.0	33.0	40.0	18.0	41.0
45	33.748935808329534	37.0	33.0	40.0	20.0	41.0
46	33.50811692578877	36.0	33.0	40.0	20.0	41.0
47	33.52166652492907	36.0	33.0	40.0	20.0	41.0
48	33.44641004429944	36.0	33.0	40.0	20.0	41.0
49	33.397764153787875	36.0	33.0	40.0	19.0	41.0
50	33.239032988782114	36.0	33.0	39.0	18.0	40.0
51	30.812878536030986	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	54.0
10	45.0
11	57.0
12	59.0
13	52.0
14	91.0
15	123.0
16	240.0
17	409.0
18	708.0
19	1147.0
20	1685.0
21	2369.0
22	3566.0
23	5037.0
24	7369.0
25	11106.0
26	14526.0
27	16022.0
28	16141.0
29	16667.0
30	18982.0
31	23168.0
32	31128.0
33	43546.0
34	58721.0
35	63319.0
36	80491.0
37	105225.0
38	100688.0
39	23983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.847232637788274	18.818217662566582	22.236155457799562	19.09839424184558
2	37.937176741632975	22.574779469179802	23.279395733955948	16.20864805523128
3	30.79514793539858	21.887944830572025	32.5853711334627	14.731536100566691
4	27.22846297169629	22.55993567690012	32.780041284297276	17.43156006710631
5	24.807378601745693	26.19852025945712	30.193510479562725	18.800590659234462
6	24.24532459198899	32.026718826103426	30.171863282488193	13.556093299419391
7	72.40260694101912	5.350568625480298	18.195860746673674	4.050963686826908
8	73.13644692184589	6.047762994116601	16.338376614842247	4.477413469195265
9	67.15115155357296	6.20083960200082	18.070925494986355	8.57708334943988
10	39.061284761146375	21.320942890055434	24.94731226854894	14.670460080249251
11	31.411474560677867	21.555505732641652	29.39751211856479	17.63550758811569
12	27.655995113918376	19.950366069564815	34.04562919897639	18.348009617540413
13	24.139562571996258	20.520305843970096	36.520831561613335	18.81930002242031
14	20.2192551817978	24.164456848631975	34.86296551137637	20.75332245819385
15	19.608804224295888	22.06653420643695	40.29331952035996	18.031342048907202
16	23.66146876232151	21.645960091845964	35.016505987769335	19.676065158063196
17	22.726619094374048	20.972423017155403	34.767872467084665	21.533085421385884
18	23.786094768336337	21.58565718570976	34.565471174437754	20.062776871516157
19	24.09472194948472	23.912421625549875	32.135264057148596	19.857592367816803
20	25.771915854252512	23.399073809210883	33.13923013289833	17.689780203638275
21	24.453601552413275	24.800575196950838	32.759012578567734	17.986810672068156
22	23.14440999791259	22.24945302171678	32.971773601243164	21.634363379127464
23	22.940307854066965	23.63719297703078	33.82900260539479	19.593496563507465
24	23.873302047979468	21.10137846258514	33.79313010738556	21.232189382049835
25	22.301870163204406	23.200692710306388	32.20298885942465	22.294448267064563
26	20.520769712478838	22.143227133215305	34.42986694704941	22.90613620725645
27	21.574060472991256	23.538852853177886	32.99713174638762	21.889954927443235
28	18.94531763396136	24.449426735834614	34.947698825639556	21.657556804564464
29	22.87892258807703	23.524009060898205	32.79055563716205	20.80651271386271
30	22.302643277385638	22.592870341020664	34.98341670081254	20.121069680781154
31	24.07647645480761	23.346656667723256	31.618359915575933	20.958506961893203
32	25.498851925440867	23.888764331604133	31.46945812427037	19.14292561868462
33	23.289446218311983	22.92376321058857	30.447091931007293	23.339698640092156
34	22.13410438587675	24.227079097311883	32.57794923732286	21.06086727948851
35	21.677348527604043	25.5135410948843	31.162377171484458	21.6467332060272
36	22.444277795387602	28.361693738548244	29.445909066310005	19.74811939975415
37	23.60132047902155	26.997765700016235	29.863236101339808	19.537677719622412
38	22.575552583361038	28.06404477877338	27.077705706355772	22.282696931509815
39	24.708883855056555	24.376754002798673	27.977919858983974	22.936442283160797
40	23.29501264041686	23.67121000100505	30.19459283941645	22.839184519161634
41	20.821201883306145	25.55528926067091	28.947250419414445	24.676258436608503
42	21.429488121100608	25.888037604273773	29.872822717187102	22.809651557438517
43	23.573333745660896	23.68883700433717	29.012965124819285	23.72486412518265
44	21.728528686401695	24.01833826837886	30.11511670158566	24.13801634363379
45	22.194561914849203	23.166675686332113	29.970698972531252	24.66806342628743
46	23.387631719328628	25.150022806868343	28.854322094830188	22.60802337897284
47	20.64879742089109	23.733213758339968	32.81838774768646	22.799601073082485
48	21.47788506884582	24.095958932174693	31.326586623578436	23.099569375401053
49	22.032053313953938	22.62240330274378	33.26911331534554	22.076430067956736
50	21.11204743828616	22.85480142562255	31.619287652593414	24.413863483497877
51	20.392896626902825	22.69105584203731	31.004352632840344	25.911694898219515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	212.0
1	705.5
2	1199.0
3	11481.0
4	21763.0
5	14913.5
6	8064.0
7	7261.0
8	6458.0
9	6185.5
10	5913.0
11	5638.0
12	5363.0
13	5182.0
14	5001.0
15	4588.0
16	4175.0
17	3796.5
18	3418.0
19	3358.5
20	3299.0
21	3234.0
22	3169.0
23	3064.0
24	2959.0
25	3241.5
26	3792.0
27	4060.0
28	4548.5
29	5037.0
30	6295.0
31	7553.0
32	8985.0
33	10417.0
34	11354.0
35	12291.0
36	13343.0
37	14395.0
38	16128.5
39	17862.0
40	19972.5
41	22083.0
42	24059.0
43	26035.0
44	30152.5
45	34270.0
46	45462.5
47	56655.0
48	52379.0
49	48103.0
50	48497.0
51	48891.0
52	45858.0
53	42825.0
54	40367.0
55	37909.0
56	35835.0
57	33761.0
58	32649.5
59	31538.0
60	32012.0
61	32486.0
62	29867.0
63	27248.0
64	23809.5
65	20371.0
66	16789.0
67	13207.0
68	10986.0
69	8765.0
70	7263.5
71	5762.0
72	4945.5
73	4129.0
74	3473.0
75	2236.0
76	1655.0
77	1309.0
78	963.0
79	724.5
80	486.0
81	384.0
82	282.0
83	219.5
84	157.0
85	117.5
86	78.0
87	67.5
88	57.0
89	42.0
90	27.0
91	22.5
92	18.0
93	14.5
94	11.0
95	9.5
96	8.0
97	6.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	646735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.710896199376194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.32945517486668	26.06845236554245
2	7.503071667382318	5.058705403112115
3	2.696574762446961	2.727118557321211
4	1.4626280897047899	1.9722601484132025
5	1.0058402918980949	1.6953888836663464
6	0.8427015997647694	1.7044935694031045
7	0.7139608418766934	1.6847781881647115
8	0.658164150354476	1.7749842683800292
9	0.5762617203250939	1.7483669133797877
>10	7.084183089151665	46.98433917518175
>50	0.09501962232488151	2.1722069662483583
>100	0.027015382817489338	1.5772602989544051
>500	0.002794694774223035	0.626350555752028
>1k	0.0018631298494820236	2.418413451915953
>5k	0.0	0.0
>10k+	4.657824623705059E-4	1.7868812545645725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11380	1.7596078764872785	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	4686	0.7245626106519673	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	4562	0.7053893789573783	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	4319	0.6678160297494338	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	1835	0.28373290451266747	No Hit
GAACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCT	814	0.1258629887048018	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCT	779	0.12045118943616784	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12246128630737474	0.0
2	0.0	0.0	0.0	0.7180684515296064	0.0
3	0.0	0.0	0.0	0.8966578273945279	0.0
4	0.0	0.0	0.0	1.260176115410485	0.0
5	0.0	0.0	0.0	2.7205888037604273	0.0
6	0.0	0.0	0.0	3.1759530565069154	0.0
7	0.0	0.0	0.0	3.73831631193611	0.0
8	0.0	0.0	0.0	4.510966624660796	0.0
9	0.0	0.0	0.0	4.816655971920493	0.0
10	0.0	0.0	0.0	6.153215768436841	0.0
11	0.0	0.0	0.0	6.887055749263609	0.0
12	0.0	0.0	0.0	8.253612376011812	0.0
13	0.0	0.0	0.0	8.541520097103142	0.0
14	0.0	0.0	0.0	8.666609971626709	0.0
15	0.0	0.0	0.0	8.94740504225069	0.0
16	0.0	0.0	0.0	9.345558845585904	0.0
17	0.0	0.0	0.0	9.808808862980973	0.0
18	0.0	0.0	0.0	10.338082831453377	0.0
19	0.0	0.0	0.0	10.660935313536456	0.0
20	0.0	0.0	0.0	10.972036460064787	0.0
21	0.0	0.0	0.0	11.342822021384338	0.0
22	0.0	0.0	0.0	11.711597485832682	0.0
23	0.0	0.0	0.0	12.065219912328851	0.0
24	0.0	0.0	0.0	12.367507557191121	0.0
25	0.0	0.0	0.0	12.650931216031296	0.0
26	0.0	0.0	0.0	12.902502570604653	0.0
27	0.0	0.0	0.0	13.158094118920424	0.0
28	0.0	0.0	0.0	13.419252089341075	0.0
29	0.0	0.0	0.0	13.680100814089233	0.0
30	0.0	0.0	0.0	13.99970621661113	0.0
31	1.5462283624668527E-4	0.0	0.0	14.301839238637154	0.0
32	1.5462283624668527E-4	0.0	0.0	14.593767153470896	0.0
33	1.5462283624668527E-4	0.0	0.0	14.894508569970698	0.0
34	1.5462283624668527E-4	0.0	0.0	15.183807896588247	0.0
35	1.5462283624668527E-4	0.0	0.0	15.478209776801936	0.0
36	1.5462283624668527E-4	0.0	0.0	15.788847054821527	0.0
37	1.5462283624668527E-4	0.0	0.0	16.080311101146528	0.0
38	1.5462283624668527E-4	0.0	0.0	16.410276233696955	0.0
39	1.5462283624668527E-4	0.0	0.0	16.776886978437844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	25	3.8889062E-5	45.000004	1
CTTCGCA	20	7.030996E-4	45.0	30
AACGACC	20	7.030996E-4	45.0	40
CGTTTTT	6285	0.0	43.31742	1
TTTACGG	90	0.0	42.500004	2
GACCTAT	55	6.002665E-11	40.909092	33
AAACACG	215	0.0	39.76744	40
CACGACC	225	0.0	39.000004	27
GTTTACG	35	6.244052E-6	38.571426	1
GAAACGT	35	6.244052E-6	38.571426	31
CGAAAGG	100	0.0	38.250004	2
ATTGCGG	65	9.094947E-12	38.07692	2
AACACGT	215	0.0	37.67442	41
CGTCCCG	30	1.1393698E-4	37.500004	41
ATCGAGT	30	1.1393698E-4	37.500004	23
TCAAACG	30	1.1393698E-4	37.500004	1
CACGTGA	230	0.0	37.173912	43
TACGAAT	55	2.744855E-9	36.81818	12
CGAATAT	55	2.744855E-9	36.81818	14
GTACGAG	55	2.744855E-9	36.81818	1
>>END_MODULE