FastQCFastQC Report
Sat 18 Jun 2016
SRR3552279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466930
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG102352.19197738419035No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC98552.11059473582764No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC96072.0574818495277665No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54961.1770500931617158No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC30580.6549161544557No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT17140.36707857708864283No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT14110.30218662326258755No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC13780.29511918274687854No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA10240.21930482085109115No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT5280.11307904825134389No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC4950.10601160773563488No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC207.0289784E-445.025
ACGATTG253.8872357E-545.01
TGATTCG207.0289784E-445.015
TACGGCT11350.043.4140977
ACGGCTG11500.042.8478248
CGTTTTT28850.042.1143881
CTACGAA700.041.78571311
CGAATAT650.041.5384614
CGAATGG556.002665E-1140.9090922
GAATCTG13900.040.7913671
GATACCT12500.040.55
ATGATAC31500.040.53
CCTCGAT501.0786607E-940.515
TGATACC12600.040.3571434
TATGCGG451.924127E-840.0000042
AATGATA31850.039.913662
ATACGGC18850.039.8673746
GAATGAT32650.039.624811
CGGACGG403.4523327E-739.3752
AATGACT2500.038.72