##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466930
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.03378236566509	30.0	28.0	31.0	16.0	33.0
2	28.53995888034609	31.0	28.0	31.0	16.0	33.0
3	28.27688946951363	31.0	27.0	33.0	16.0	34.0
4	32.60959672756088	35.0	32.0	35.0	28.0	37.0
5	33.418199730152274	35.0	33.0	35.0	28.0	37.0
6	33.23809350437967	35.0	33.0	35.0	28.0	37.0
7	33.99764846979205	35.0	33.0	35.0	30.0	37.0
8	33.95860193176708	35.0	35.0	37.0	30.0	37.0
9	35.30961171910136	37.0	34.0	39.0	30.0	39.0
10	35.29546612982674	37.0	34.0	39.0	30.0	39.0
11	35.579433748099284	37.0	35.0	39.0	30.0	39.0
12	35.571809478936885	37.0	35.0	39.0	30.0	39.0
13	35.5593793502238	37.0	35.0	39.0	30.0	39.0
14	36.74575632321761	38.0	36.0	40.0	31.0	41.0
15	36.76470991369156	38.0	35.0	40.0	31.0	41.0
16	36.60658985286874	38.0	35.0	40.0	31.0	41.0
17	35.68865997044525	38.0	33.0	40.0	29.0	41.0
18	35.39362645364402	37.0	33.0	39.0	28.0	40.0
19	34.91208318163322	37.0	33.0	39.0	27.0	40.0
20	35.44793437988564	37.0	34.0	39.0	29.0	40.0
21	35.930972522647934	38.0	34.0	39.0	30.0	40.0
22	36.38354357184161	38.0	35.0	40.0	31.0	41.0
23	36.58211295054933	38.0	35.0	40.0	31.0	41.0
24	36.55106332855032	38.0	35.0	40.0	31.0	41.0
25	35.816724134238534	38.0	34.0	40.0	29.0	41.0
26	35.98120489152549	38.0	35.0	40.0	30.0	41.0
27	36.27281177050093	38.0	35.0	40.0	30.0	41.0
28	36.22011222238879	38.0	35.0	40.0	30.0	41.0
29	36.24872678988285	38.0	35.0	40.0	30.0	41.0
30	35.684027584434496	38.0	35.0	40.0	29.0	41.0
31	35.93226179512989	38.0	35.0	40.0	30.0	41.0
32	35.83228963656222	38.0	35.0	40.0	29.0	41.0
33	35.44605615402737	38.0	35.0	40.0	27.0	41.0
34	35.528342578116636	38.0	35.0	40.0	27.0	41.0
35	35.242734456984984	38.0	35.0	40.0	25.0	41.0
36	35.11852097744844	38.0	34.0	40.0	25.0	41.0
37	35.03992675561647	38.0	34.0	40.0	24.0	41.0
38	34.787876127042594	38.0	34.0	40.0	23.0	41.0
39	34.731295911592746	38.0	34.0	40.0	24.0	41.0
40	34.434726832715825	38.0	34.0	40.0	23.0	41.0
41	34.40183110958816	38.0	34.0	40.0	23.0	41.0
42	34.46353629023622	38.0	34.0	40.0	23.0	41.0
43	34.321328678816954	37.0	33.0	40.0	23.0	41.0
44	34.24279013984965	37.0	33.0	40.0	23.0	41.0
45	34.1819394770094	37.0	33.0	40.0	23.0	41.0
46	33.90385068425674	37.0	33.0	40.0	22.0	41.0
47	33.77770972094318	37.0	33.0	40.0	21.0	41.0
48	33.771543914505386	36.0	33.0	40.0	21.0	40.0
49	33.74119032831474	36.0	33.0	40.0	21.0	40.0
50	33.593907009616004	36.0	33.0	39.0	20.0	40.0
51	30.764187351423125	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	23.0
10	42.0
11	32.0
12	15.0
13	28.0
14	62.0
15	96.0
16	162.0
17	369.0
18	566.0
19	873.0
20	1205.0
21	1783.0
22	2545.0
23	3452.0
24	4751.0
25	6715.0
26	8762.0
27	9886.0
28	10765.0
29	11677.0
30	13934.0
31	17718.0
32	23366.0
33	31524.0
34	42064.0
35	48584.0
36	62644.0
37	76753.0
38	69996.0
39	16528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.43771014927291	18.810528344719764	20.715310646135396	16.036450859871927
2	33.18484569421541	29.425181504722335	21.499368213650868	15.890604587411389
3	29.33523226179513	28.07165956353201	28.0560255284518	14.53708264622106
4	26.586212066048443	21.485875827211785	35.410018632343174	16.51789347439659
5	28.222859957595357	26.135823356820083	26.059152335467843	19.58216435011672
6	22.59010986657529	36.54701989591588	27.18094789368856	13.681922343820274
7	70.61122652217678	5.619043539716874	19.470584455914164	4.299145482192192
8	69.73079476581073	10.710384854260809	14.532799348938813	5.026021030989656
9	64.62638939455593	6.432441693615745	14.942925063714046	13.998243848114278
10	39.18060522990598	21.649497783393656	23.037286102841968	16.132610883858394
11	30.157197010258496	22.965326708500204	27.09121281562547	19.786263465615832
12	25.4605615402737	20.60030411410704	32.71346882830402	21.225665517315228
13	21.62294134024372	22.892296489837875	37.34542650932688	18.139335660591524
14	21.09673826911957	26.554943995888035	30.687255048936674	21.661062686055725
15	19.639132203970615	22.745593557920888	38.885057717430875	18.73021652067762
16	21.631293769944104	23.759235859764846	30.1400638211295	24.469406549161548
17	21.43918788683529	22.28599575953569	33.24309853725398	23.031717816375046
18	22.656929304178355	23.258090077741848	31.804338980146916	22.28064163793288
19	22.747092711969675	24.988542179769986	29.39091512646435	22.873449981795986
20	23.714689568029467	24.250315893174566	33.37930739082946	18.655687147966503
21	23.69884136808515	27.249694815068644	30.05289872143576	18.99856509541045
22	21.697470712954832	21.44218619493286	30.475445998329516	26.384897093782794
23	21.84053284218191	27.069582164350116	31.03591544771165	20.053969545756324
24	24.11003790718095	22.216606343563274	29.179962735313648	24.493393013942132
25	21.09695243398368	29.328164821279422	28.481356948579016	21.093525796157884
26	21.825755466558157	22.427558734713983	30.583385089842157	25.1633007088857
27	23.959694172574046	25.423082689054034	28.028184096117194	22.589039042254726
28	20.870151842888657	23.78879061101236	33.1276636754974	22.21339387060159
29	27.509690960101086	22.97946158953162	26.986272032210394	22.524575418156896
30	22.149572741096097	28.272117876341206	30.021844816139463	19.55646456642323
31	27.90289765061144	23.242670207525755	26.652389009059174	22.20204313280363
32	27.4321632792924	26.271175550939112	26.199644486325575	20.097016683442913
33	21.387359989720085	27.22742166920095	25.965348124986615	25.41987021609235
34	25.853554065919944	23.587903968474933	29.91497654894738	20.643565416657744
35	21.779281691045767	26.55965562289851	25.86126400102799	25.799798685027735
36	26.958002270147556	28.773263658364208	24.52166277600497	19.747071295483263
37	24.584841410918123	27.34178570663697	28.23035572783929	19.843017154605615
38	23.920287837577366	28.615638318377485	24.212194547362557	23.25187929668259
39	26.433940847664534	24.002312980532416	28.952519649626286	20.61122652217677
40	22.330113721542844	25.348767481207034	29.070952819480432	23.250165977769687
41	20.242006296447006	28.59100935900456	28.156040519992292	23.010943824556144
42	21.296125757608205	24.023729466943653	30.453387017325937	24.226757758122204
43	24.861328250487226	25.20163621956182	25.786734628316875	24.15030090163408
44	23.733964405799586	23.296853918146187	28.940954746964216	24.028226929090014
45	21.96046516608485	22.428629559034544	28.16032381727454	27.450581457606066
46	26.074358040819824	25.766817295954425	26.23755166727347	21.921272995952286
47	20.414409012057483	23.418927890690252	35.298010408412395	20.86865268883987
48	23.16899749427109	25.248538324802432	26.958644764739898	24.623819416186578
49	21.07018182596963	23.067269183817704	32.892296489837875	22.970252500374787
50	22.60595806651961	22.68648405542587	30.67676097059516	24.03079690745936
51	22.94519521127364	22.270147559591376	26.59949028762341	28.185166941511575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	133.0
1	423.0
2	713.0
3	5089.0
4	9465.0
5	6708.5
6	3952.0
7	3560.0
8	3168.0
9	3014.5
10	2861.0
11	2831.0
12	2801.0
13	2620.5
14	2440.0
15	2259.0
16	2078.0
17	1917.5
18	1757.0
19	1633.0
20	1509.0
21	1511.0
22	1513.0
23	1609.5
24	1706.0
25	2103.5
26	2839.5
27	3178.0
28	3631.5
29	4085.0
30	4914.5
31	5744.0
32	6415.0
33	7086.0
34	7849.0
35	8612.0
36	9653.0
37	10694.0
38	11610.0
39	12526.0
40	14027.0
41	15528.0
42	17395.0
43	19262.0
44	21957.5
45	24653.0
46	45037.5
47	65422.0
48	51417.5
49	37413.0
50	36782.0
51	36151.0
52	33133.5
53	30116.0
54	27993.5
55	25871.0
56	24795.5
57	23720.0
58	22400.0
59	21080.0
60	20154.0
61	19228.0
62	17996.0
63	16764.0
64	15188.0
65	13612.0
66	11722.5
67	9833.0
68	8525.5
69	7218.0
70	6011.0
71	4804.0
72	3930.0
73	3056.0
74	2522.0
75	1626.5
76	1265.0
77	957.0
78	649.0
79	494.0
80	339.0
81	265.5
82	192.0
83	150.0
84	108.0
85	85.5
86	63.0
87	45.0
88	27.0
89	22.5
90	18.0
91	14.5
92	11.0
93	11.5
94	12.0
95	7.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	466930.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.69816158590429
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85460259730586	25.224624937726638
2	7.080820779768611	4.772212855949428
3	2.878621726186431	2.910127802211752
4	1.933356618460727	2.6060225492826836
5	1.5339983852313865	2.5846462729021757
6	1.286544521758608	2.601251110900892
7	1.1587915976234586	2.733440255077244
8	1.1033687177421336	2.9745197871445153
9	0.9471124541318752	2.872435366742246
>10	7.118702103214888	37.51097007894646
>50	0.06723212330375865	1.5262294497791946
>100	0.030383748031506317	2.0316322935542064
>500	6.464627240746025E-4	0.11502271616948541
>1k	0.003232313620373012	1.8702083680208943
>5k	0.0019393881722238075	5.437001799541698
>10k+	6.464627240746025E-4	2.229654356050536
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	10235	2.19197738419035	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	9855	2.11059473582764	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	9607	2.0574818495277665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5496	1.1770500931617158	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	3058	0.6549161544557	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT	1714	0.36707857708864283	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT	1411	0.30218662326258755	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC	1378	0.29511918274687854	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA	1024	0.21930482085109115	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	528	0.11307904825134389	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	495	0.10601160773563488	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14241963463474183	0.0
2	0.0	0.0	0.0	1.1841175336774248	0.0
3	0.0	0.0	0.0	1.425695500396205	0.0
4	0.0	0.0	0.0	1.9928040605658235	0.0
5	0.0	0.0	0.0	5.1131861306834	0.0
6	0.0	0.0	0.0	5.607478637054805	0.0
7	0.0	0.0	0.0	6.394962842396077	0.0
8	0.0	0.0	0.0	7.26854132311053	0.0
9	0.0	0.0	0.0	7.501766860128927	0.0
10	0.0	0.0	0.0	10.422547276893752	0.0
11	0.0	0.0	0.0	11.033987963934637	0.0
12	0.0	0.0	0.0	13.905938791681837	0.0
13	0.0	0.0	0.0	14.218619493285932	0.0
14	0.0	0.0	0.0	14.383954768380699	0.0
15	0.0	0.0	0.0	14.819137772257083	0.0
16	0.0	0.0	0.0	15.227764332983531	0.0
17	0.0	0.0	0.0	15.629751782922494	0.0
18	0.0	0.0	0.0	16.070717238129912	0.0
19	0.0	0.0	0.0	16.36283811277922	0.0
20	0.0	0.0	0.0	16.624975906452786	0.0
21	0.0	0.0	0.0	16.920095089199666	0.0
22	0.0	0.0	0.0	17.25140813398154	0.0
23	0.0	0.0	0.0	17.561518857216285	0.0
24	0.0	0.0	0.0	17.84014734542651	0.0
25	0.0	0.0	0.0	18.07380121217313	0.0
26	0.0	0.0	0.0	18.27682950335168	0.0
27	0.0	0.0	0.0	18.513481678195877	0.0
28	0.0	0.0	0.0	18.73214400445463	0.0
29	0.0	0.0	0.0	18.967297025250037	0.0
30	0.0	0.0	0.0	19.253207118840084	0.0
31	0.0	0.0	0.0	19.490501788276614	0.0
32	0.0	0.0	0.0	19.72072901719744	0.0
33	0.0	0.0	0.0	19.964448632557342	0.0
34	0.0	0.0	0.0	20.21095239115071	0.0
35	0.0	0.0	0.0	20.469235217270256	0.0
36	0.0	0.0	0.0	20.70781487589146	0.0
37	0.0	0.0	0.0	20.94211123723042	0.0
38	0.0	0.0	0.0	21.17747842288994	0.0
39	0.0	0.0	0.0	21.429122138222002	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAC	20	7.0289784E-4	45.0	25
ACGATTG	25	3.8872357E-5	45.0	1
TGATTCG	20	7.0289784E-4	45.0	15
TACGGCT	1135	0.0	43.414097	7
ACGGCTG	1150	0.0	42.847824	8
CGTTTTT	2885	0.0	42.114388	1
CTACGAA	70	0.0	41.785713	11
CGAATAT	65	0.0	41.53846	14
CGAATGG	55	6.002665E-11	40.909092	2
GAATCTG	1390	0.0	40.791367	1
GATACCT	1250	0.0	40.5	5
ATGATAC	3150	0.0	40.5	3
CCTCGAT	50	1.0786607E-9	40.5	15
TGATACC	1260	0.0	40.357143	4
TATGCGG	45	1.924127E-8	40.000004	2
AATGATA	3185	0.0	39.91366	2
ATACGGC	1885	0.0	39.867374	6
GAATGAT	3265	0.0	39.62481	1
CGGACGG	40	3.4523327E-7	39.375	2
AATGACT	250	0.0	38.7	2
>>END_MODULE