FastQCFastQC Report
Sat 18 Jun 2016
SRR3552275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434187
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56811.3084224078565225No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG46101.0617544974861064No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC39720.9148132026062503No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC39070.8998426945072054No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC19210.4424360932040803No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT8520.19622881385209598No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT8100.18655556246502084No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGGTCTTCGTATGCCGTCTTC7420.1708941078383277No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC5560.12805542312413776No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTC5260.12114595784765551No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT5000.11515775460803755No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4820.1110120754421482No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTA4490.10341166363801771No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGG207.02843E-445.00000424
ACGTTAC207.02843E-445.00000429
CGGTTAG207.02843E-445.00000432
ATACGCG253.8867824E-545.01
ATACGAG253.8867824E-545.01
CGTTTTT30750.042.804881
TACGAGG603.6379788E-1241.2500042
GCGTAAG556.002665E-1140.9090921
TACGGCT6000.040.8757
ACACGAC1550.040.6451626
CGTAAGG1000.040.52
CGGTAGT451.9235813E-840.012
TTAACGG403.451587E-739.3750042
ACGTAAG403.451587E-739.3750041
CACGACC1550.039.19354627
GTCAAGC1550.039.19354616
ACGACCA1550.039.19354628
GGCGATA1100.038.863648
AGGGTAA700.038.5714266
TTGCACG700.038.5714261