##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552275_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434187
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.055879148846003	30.0	28.0	31.0	16.0	33.0
2	28.613102188688284	31.0	28.0	33.0	16.0	34.0
3	28.32201102290027	31.0	27.0	33.0	16.0	34.0
4	32.55332379827125	35.0	32.0	35.0	28.0	37.0
5	33.45246633363044	35.0	33.0	35.0	28.0	37.0
6	33.30414084254019	35.0	33.0	35.0	28.0	37.0
7	34.15656848316509	35.0	33.0	36.0	31.0	37.0
8	34.27903184572546	35.0	35.0	37.0	31.0	37.0
9	35.557923198990295	37.0	34.0	39.0	30.0	39.0
10	35.46762800360214	37.0	34.0	39.0	30.0	39.0
11	35.70559689718946	37.0	35.0	39.0	31.0	39.0
12	35.63587117992939	37.0	35.0	39.0	30.0	39.0
13	35.715634047081096	37.0	35.0	39.0	30.0	39.0
14	36.76893366222388	38.0	36.0	40.0	31.0	41.0
15	36.927832938342235	39.0	35.0	40.0	31.0	41.0
16	36.69206586102302	38.0	35.0	40.0	31.0	41.0
17	35.76034289373012	38.0	34.0	40.0	29.0	41.0
18	35.520211337511256	38.0	33.0	39.0	29.0	41.0
19	34.99905800956731	37.0	33.0	39.0	27.0	40.0
20	35.5739923120683	37.0	34.0	39.0	30.0	40.0
21	35.93279854072093	38.0	34.0	39.0	30.0	40.0
22	36.35412852066045	38.0	35.0	40.0	31.0	41.0
23	36.553655452604524	38.0	35.0	40.0	31.0	41.0
24	36.4928452487062	38.0	35.0	40.0	31.0	41.0
25	35.790885033407264	38.0	34.0	40.0	29.0	41.0
26	35.967417264911205	38.0	35.0	40.0	30.0	41.0
27	36.214230274052426	38.0	35.0	40.0	30.0	41.0
28	36.202120284577845	38.0	35.0	40.0	30.0	41.0
29	36.16990144799361	38.0	35.0	40.0	30.0	41.0
30	35.46546073466041	38.0	34.0	40.0	27.0	41.0
31	35.67385942001948	38.0	35.0	40.0	29.0	41.0
32	35.54495413266634	38.0	34.0	40.0	29.0	41.0
33	35.2362714682844	38.0	34.0	40.0	25.0	41.0
34	35.2268423513371	38.0	34.0	40.0	26.0	41.0
35	34.767812025694	38.0	34.0	40.0	23.0	41.0
36	34.630944731187256	38.0	34.0	40.0	23.0	41.0
37	34.57461416394319	38.0	34.0	40.0	23.0	41.0
38	34.27157653269214	38.0	33.0	40.0	21.0	41.0
39	34.2198499724773	37.0	33.0	40.0	22.0	41.0
40	33.94344142040181	37.0	33.0	40.0	20.0	41.0
41	33.892527873934505	37.0	33.0	40.0	20.0	41.0
42	33.89850456139866	37.0	33.0	40.0	20.0	41.0
43	33.77581779279435	37.0	33.0	40.0	20.0	41.0
44	33.70049080235014	37.0	33.0	40.0	20.0	41.0
45	33.59788524299438	37.0	33.0	40.0	20.0	41.0
46	33.329692045132624	36.0	33.0	40.0	18.0	41.0
47	33.347136141800654	36.0	33.0	40.0	19.0	41.0
48	33.240007185843886	36.0	33.0	39.0	18.0	40.0
49	33.209296915845016	36.0	32.0	39.0	18.0	40.0
50	33.05820994179927	35.0	32.0	39.0	18.0	40.0
51	30.314576438262776	34.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	31.0
10	30.0
11	40.0
12	30.0
13	30.0
14	48.0
15	101.0
16	139.0
17	276.0
18	541.0
19	803.0
20	1257.0
21	1890.0
22	2690.0
23	3757.0
24	5011.0
25	7226.0
26	9446.0
27	10524.0
28	10833.0
29	11654.0
30	13271.0
31	16489.0
32	21369.0
33	29135.0
34	38907.0
35	42983.0
36	55189.0
37	69539.0
38	65782.0
39	15158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17672799968677	19.102598649890485	21.865233182937306	17.85544016748544
2	34.890266175634	25.671427288242164	22.668343363573758	16.769963172550074
3	29.819409609223673	24.230343147077182	30.575996056998484	15.374251186700661
4	26.771874791276566	22.30168107290177	32.94847611743327	17.977968018388392
5	26.554687266085814	25.81353195742848	28.146628065787322	19.485152710698387
6	23.946594439722976	33.300398215515436	28.639733571018937	14.113273773742648
7	72.6689191523468	5.075693192103863	17.79026087837729	4.465126777172048
8	72.51299555260752	7.467980386331235	15.03292360204244	4.98610045901881
9	66.83894266756029	6.140441791209779	16.252674538850773	10.76794100237916
10	38.826127912627506	21.923963637787406	23.600890860389647	15.649017589195438
11	29.999055706412214	21.458495993661717	29.255597242662724	19.286851057263345
12	25.84462455117265	20.004053552962205	33.89253938970997	20.258782506155182
13	23.12713185793218	21.658870486679703	36.87167050141989	18.34232715396822
14	21.194554420100093	24.59999953936898	32.88237556628826	21.323070474242662
15	19.537434331290434	21.767579407029693	38.9263151591365	19.768671102543376
16	22.54673677470767	22.715558042963053	32.62925882165979	22.108446360669483
17	22.499522095318376	21.812951562345255	33.12190369587298	22.56562264646339
18	23.277067254431845	21.81986102762174	33.72302717492693	21.180044543019484
19	22.7950168936426	24.038490327900192	31.20683023674131	21.959662541715897
20	24.43463300375184	23.383703335198888	33.33333333333333	18.84833032771594
21	24.285158238270608	25.53577145331389	31.640053709576748	18.53901659883875
22	22.934127461209112	21.133520810157837	31.450964676510353	24.481387052122702
23	22.02000520513051	25.435123575786474	32.06245235347903	20.48241886560399
24	23.653172480981695	21.399765538811618	31.685886495910747	23.261175484295936
25	21.840128792432754	25.29117638252642	31.223643268914085	21.645051556126738
26	21.57756911192643	22.77037313415648	31.712833410489026	23.939224343428062
27	22.2376533613397	23.683574128198217	30.966841476138164	23.11193103432392
28	20.571320652161397	23.5944420261316	33.52541646801954	22.308820853687468
29	25.354973778579275	22.050637167856245	30.43389138781216	22.160497665752313
30	22.730758866571314	23.088208536874667	34.445987558356194	19.735045038197825
31	26.620096870703176	22.32724609442475	29.766206726594763	21.28645030827731
32	26.455881912632112	23.345010329650588	29.709779426836825	20.489328330880475
33	23.117458606545107	21.68719929431328	31.48850610451257	23.706835994629042
34	24.650899266905736	22.41776008954667	32.24325002821365	20.688090615333945
35	22.312966532853356	22.59072703696794	31.347783328381553	23.748523101797154
36	25.790039775488445	24.285388553779825	30.567013752139054	19.35755791859268
37	24.439469629445377	23.90053133787976	31.31784231218346	20.3421567204914
38	23.94636412421376	25.23958570846202	28.57985153862276	22.234198628701456
39	25.225766777909058	22.44724047472633	30.832567534265188	21.494425213099426
40	24.04194506053843	22.073208087759422	31.360450681388436	22.52439617031371
41	21.007768542125856	23.70983009624885	31.3470923818539	23.93530897977139
42	22.360872158770302	22.618134582564657	31.326824617042888	23.694168641622156
43	24.19809897578693	21.806963359105637	30.123886712407327	23.871050952700106
44	23.038690702393207	22.308129907159817	30.584287415330262	24.068891975116713
45	22.826570118405204	21.627777892935534	30.02807546057344	25.517576528085822
46	24.91990778167011	23.742534898557537	29.28047131765806	22.057086002114296
47	21.1802748585287	22.732601390645044	34.176979043591814	21.91014470723444
48	22.75701483462195	23.68449539023508	30.09026064806178	23.468229127081187
49	22.14506652663484	21.90162303339345	33.626064345546965	22.32724609442475
50	22.10199752641143	21.857632771133176	31.95904068984101	24.08132901261438
51	21.975554311851806	21.471854293196248	29.70125775299583	26.851333641956117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	488.5
2	787.0
3	5621.5
4	10456.0
5	7252.5
6	4049.0
7	3662.0
8	3275.0
9	3157.0
10	3039.0
11	2926.0
12	2813.0
13	2792.0
14	2771.0
15	2657.5
16	2544.0
17	2395.5
18	2247.0
19	2081.0
20	1915.0
21	1894.5
22	1874.0
23	1848.5
24	1823.0
25	2097.0
26	2491.0
27	2611.0
28	3248.5
29	3886.0
30	4314.0
31	4742.0
32	5371.0
33	6000.0
34	7259.0
35	8518.0
36	8802.5
37	9087.0
38	10364.0
39	11641.0
40	13276.5
41	14912.0
42	16294.5
43	17677.0
44	20390.5
45	23104.0
46	32893.5
47	42683.0
48	38998.5
49	35314.0
50	35610.0
51	35906.0
52	33105.5
53	30305.0
54	28195.5
55	26086.0
56	24592.0
57	23098.0
58	22417.0
59	21736.0
60	20524.0
61	19312.0
62	17866.0
63	16420.0
64	14596.5
65	12773.0
66	11266.0
67	9759.0
68	8289.5
69	6820.0
70	5706.5
71	4593.0
72	3701.0
73	2809.0
74	2372.0
75	1492.0
76	1049.0
77	826.5
78	604.0
79	446.5
80	289.0
81	223.5
82	158.0
83	127.0
84	96.0
85	72.5
86	49.0
87	35.5
88	22.0
89	20.0
90	18.0
91	16.0
92	14.0
93	8.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434187.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.50654247805488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42005372765004	27.898319375836927
2	6.8995705405620456	5.037589300387289
3	2.629841406164943	2.880192510141242
4	1.7265089483040335	2.521154890400122
5	1.3540209638825158	2.471531191707694
6	1.1601405432829517	2.541163201432365
7	1.0339472919640165	2.6422088513907926
8	0.9534907992172622	2.7846921891247587
9	0.8897077278603261	2.9232137664168025
>10	6.8633886679659195	40.263851348750684
>50	0.04172601763414239	1.0545733628313827
>100	0.021183978183487676	1.4562486392520473
>500	0.003209693664164799	0.8169428317400446
>1k	0.002567754931331839	3.376978257422272
>5k	6.419387328329598E-4	1.3313402831655745
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5681	1.3084224078565225	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG	4610	1.0617544974861064	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGC	3972	0.9148132026062503	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC	3907	0.8998426945072054	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	1921	0.4424360932040803	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCT	852	0.19622881385209598	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCT	810	0.18655556246502084	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGGTCTTCGTATGCCGTCTTC	742	0.1708941078383277	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGC	556	0.12805542312413776	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTC	526	0.12114595784765551	No Hit
CTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTCTGCT	500	0.11515775460803755	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	482	0.1110120754421482	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTA	449	0.10341166363801771	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15039602751809705	0.0
2	0.0	0.0	0.0	1.030201272723504	0.0
3	0.0	0.0	0.0	1.2621289905040916	0.0
4	0.0	0.0	0.0	1.7229903244454579	0.0
5	0.0	0.0	0.0	3.7822412923463853	0.0
6	0.0	0.0	0.0	4.300451188082555	0.0
7	0.0	0.0	0.0	4.982185095362136	0.0
8	0.0	0.0	0.0	5.80349020122666	0.0
9	0.0	0.0	0.0	6.097142475477156	0.0
10	0.0	0.0	0.0	8.056436512378307	0.0
11	0.0	0.0	0.0	8.890639286758931	0.0
12	0.0	0.0	0.0	10.795348547975872	0.0
13	0.0	0.0	0.0	11.15464074235295	0.0
14	0.0	0.0	0.0	11.304115507834181	0.0
15	0.0	0.0	0.0	11.651891926750455	0.0
16	0.0	0.0	0.0	12.116207993330063	0.0
17	0.0	0.0	0.0	12.625435584206805	0.0
18	0.0	0.0	0.0	13.18671448016638	0.0
19	0.0	0.0	0.0	13.519290075474393	0.0
20	0.0	0.0	0.0	13.832058536989823	0.0
21	0.0	0.0	0.0	14.209545656594969	0.0
22	0.0	0.0	0.0	14.56975911300891	0.0
23	0.0	0.0	0.0	14.935500141644038	0.0
24	0.0	0.0	0.0	15.23237683302356	0.0
25	0.0	0.0	0.0	15.501155032278719	0.0
26	0.0	0.0	0.0	15.737228429225196	0.0
27	0.0	0.0	0.0	15.996333377093281	0.0
28	0.0	0.0	0.0	16.259353688618038	0.0
29	0.0	0.0	0.0	16.52030116055985	0.0
30	0.0	0.0	0.0	16.84182161142549	0.0
31	0.0	0.0	0.0	17.11889116901243	0.0
32	0.0	0.0	0.0	17.3835236891017	0.0
33	0.0	0.0	0.0	17.666120818909825	0.0
34	0.0	0.0	0.0	17.93812343529401	0.0
35	0.0	0.0	0.0	18.226017821814104	0.0
36	0.0	0.0	0.0	18.48489245417297	0.0
37	0.0	0.0	0.0	18.761040749723044	0.0
38	0.0	0.0	0.0	19.046631981150977	0.0
39	0.0	0.0	0.0	19.365388645906027	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGG	20	7.02843E-4	45.000004	24
ACGTTAC	20	7.02843E-4	45.000004	29
CGGTTAG	20	7.02843E-4	45.000004	32
ATACGCG	25	3.8867824E-5	45.0	1
ATACGAG	25	3.8867824E-5	45.0	1
CGTTTTT	3075	0.0	42.80488	1
TACGAGG	60	3.6379788E-12	41.250004	2
GCGTAAG	55	6.002665E-11	40.909092	1
TACGGCT	600	0.0	40.875	7
ACACGAC	155	0.0	40.64516	26
CGTAAGG	100	0.0	40.5	2
CGGTAGT	45	1.9235813E-8	40.0	12
TTAACGG	40	3.451587E-7	39.375004	2
ACGTAAG	40	3.451587E-7	39.375004	1
CACGACC	155	0.0	39.193546	27
GTCAAGC	155	0.0	39.193546	16
ACGACCA	155	0.0	39.193546	28
GGCGATA	110	0.0	38.86364	8
AGGGTAA	70	0.0	38.571426	6
TTGCACG	70	0.0	38.571426	1
>>END_MODULE