FastQCFastQC Report
Sat 18 Jun 2016
SRR3552273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619975
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150802.4323561433928784No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC81621.3165046977700714No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG41040.6619621758941893No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC30270.4882454937699101No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT24030.3875962740433082No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC19870.3204967942255736No Hit
TAGTGAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT14650.2362998508004355No Hit
TAGTGAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTAT11720.1890398806403484No Hit
TAGTGAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGT11660.1880720996814388No Hit
TAGTGAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC9970.1608129360054841No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT8120.1309730231057704No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT7920.12774708657607162No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.1269406024436469No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC7850.12661800879067703No Hit
TAGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT7830.12629541513770717No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC7050.11371426267188192No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT6390.10306867212387595No Hit
TAGTGAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC6240.10064921972660187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG453.8380676E-1045.0000042
CGGTCTA207.03077E-445.031
ACATACG207.03077E-445.017
CGTTTTT93900.043.7539941
TAGTGAA9250.042.3243261
AGTGAAT8900.041.2078672
TTAACGG501.0804797E-940.5000042
TCAAGCG2450.039.48979617
ACACGAC2450.038.5714326
GTTAACG356.243634E-638.5714261
TCGTAAC356.243634E-638.57142634
TCGAAGG356.243634E-638.5714262
ACGGGCT950.037.8947375
CACGACC2500.037.827
CGTTCTG1250.037.81
AGACACG2450.037.6530624
ATTACGG301.1393153E-437.5000042
CGTTCAA301.1393153E-437.5000049
TTGGTAG301.1393153E-437.5000041
GCGTAAG301.1393153E-437.5000041