##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552273_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	619975
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.982568651961774	30.0	26.0	31.0	16.0	33.0
2	28.578628170490745	31.0	28.0	33.0	16.0	33.0
3	28.182134763498528	31.0	27.0	33.0	16.0	34.0
4	31.689890721400058	35.0	30.0	35.0	19.0	37.0
5	33.12929714907859	35.0	32.0	35.0	28.0	37.0
6	33.159482237186985	35.0	33.0	35.0	28.0	37.0
7	34.012474696560346	35.0	33.0	36.0	30.0	37.0
8	34.29661518609621	35.0	35.0	37.0	31.0	37.0
9	35.57528287430945	37.0	35.0	39.0	30.0	39.0
10	35.527203516270816	37.0	35.0	39.0	30.0	39.0
11	35.80072260978265	37.0	35.0	39.0	31.0	39.0
12	35.734273156175654	37.0	35.0	39.0	30.0	39.0
13	35.892982781563774	37.0	35.0	39.0	31.0	39.0
14	36.92082745271987	38.0	36.0	40.0	31.0	41.0
15	37.10778660429856	39.0	36.0	40.0	32.0	41.0
16	36.83625307472075	38.0	35.0	40.0	31.0	41.0
17	36.00166619621759	38.0	34.0	40.0	29.0	41.0
18	35.69461026654301	38.0	34.0	39.0	29.0	40.0
19	35.10200250010081	37.0	33.0	39.0	27.0	40.0
20	35.43369329408444	37.0	34.0	39.0	30.0	40.0
21	35.65670551231904	37.0	34.0	39.0	30.0	40.0
22	36.08141134723174	38.0	35.0	40.0	31.0	41.0
23	36.286672849711685	38.0	35.0	40.0	31.0	41.0
24	36.179562079116096	38.0	35.0	40.0	31.0	41.0
25	35.488356788580184	37.0	34.0	40.0	29.0	41.0
26	35.658734626396225	37.0	34.0	40.0	30.0	41.0
27	35.95424009032622	38.0	35.0	40.0	30.0	41.0
28	35.872840033872336	38.0	35.0	40.0	30.0	41.0
29	35.88291624662285	38.0	35.0	40.0	30.0	41.0
30	35.30156377273277	38.0	34.0	40.0	27.0	41.0
31	35.330824630025404	38.0	34.0	40.0	27.0	41.0
32	34.949360861325054	38.0	34.0	40.0	25.0	41.0
33	34.54931408524537	38.0	34.0	40.0	22.0	41.0
34	34.13982660591153	38.0	33.0	40.0	20.0	41.0
35	33.73121658131376	38.0	33.0	40.0	16.0	41.0
36	33.374639299971776	37.0	33.0	40.0	15.0	41.0
37	33.284156619218514	37.0	33.0	40.0	15.0	41.0
38	33.14081535545788	37.0	33.0	40.0	13.0	41.0
39	32.930458486229284	37.0	32.0	40.0	12.0	41.0
40	32.828906004274366	37.0	32.0	40.0	11.0	41.0
41	32.7118045082463	37.0	32.0	40.0	10.0	41.0
42	32.657591031896445	37.0	32.0	40.0	10.0	41.0
43	32.573176337755555	36.0	32.0	40.0	10.0	41.0
44	32.48045485705069	36.0	31.0	40.0	10.0	41.0
45	32.46579297552321	36.0	31.0	40.0	10.0	41.0
46	32.24314851405298	36.0	31.0	40.0	10.0	40.0
47	32.23775797411186	36.0	31.0	39.0	10.0	40.0
48	32.1827234969152	36.0	31.0	39.0	10.0	40.0
49	32.11166095407073	35.0	31.0	39.0	10.0	40.0
50	31.950620589539902	35.0	31.0	39.0	10.0	40.0
51	29.57863623533207	34.0	25.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	33.0
10	45.0
11	50.0
12	36.0
13	48.0
14	73.0
15	156.0
16	327.0
17	595.0
18	1006.0
19	1640.0
20	2435.0
21	3320.0
22	4832.0
23	6749.0
24	10105.0
25	14806.0
26	19237.0
27	20076.0
28	18826.0
29	18304.0
30	19702.0
31	22936.0
32	29428.0
33	39315.0
34	50823.0
35	61404.0
36	75189.0
37	93416.0
38	85228.0
39	19819.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.72519053187629	17.379087866446227	20.470180249203594	25.42554135247389
2	41.097947497882984	23.454332836001452	20.57873301342796	14.868986652687607
3	29.859429815718375	20.82438808016452	35.04028388241461	14.275898221702487
4	24.258720109681843	20.742933182789628	38.5649421347635	16.43340457276503
5	23.991451268196297	23.648695511915804	34.59381426670431	17.766038953183596
6	21.736360337110366	30.241380700834714	34.921892011774666	13.100366950280254
7	65.94798177345861	6.167345457478124	23.69272954554619	4.191943223517077
8	65.82636396628897	5.431831928706803	23.701439574176376	5.040364530827856
9	61.641840396790194	5.45231662567039	24.19662083148514	8.709222146054277
10	33.87055929674584	21.01858945925239	30.3925158272511	14.718335416750675
11	25.82120246784145	21.455703859026574	35.70450421387959	17.01858945925239
12	22.799951610952053	19.88370498810436	39.485140529860075	17.831202871083512
13	20.904230009274567	19.51756119198355	41.527964837291826	18.050243961450057
14	19.23480785515545	22.087180934715107	39.33126335739344	19.346747852735998
15	18.46348643090447	21.394733658615266	42.87495463526756	17.26682527521271
16	21.397959595144968	21.46086535747409	38.035888543892895	19.105286503488045
17	21.098270091535948	21.43796120811323	38.31638372515021	19.147384975200612
18	21.777168434211056	21.837493447316426	37.514415903867096	18.87092221460543
19	21.21924271139965	23.300939554014274	36.100004032420664	19.37981370216541
20	22.159280616153875	23.374813500544377	36.59615307068834	17.869752812613413
21	21.491027864026776	23.94354611073027	37.133755393362634	17.431670631880316
22	20.940199201580707	20.48856808742288	37.36811968224526	21.203113028751158
23	20.677124077583773	22.912052905359086	37.70119762893665	18.709625388120486
24	21.35311907738215	21.24682446872858	37.351022218637844	20.049034235251423
25	20.891326263155772	23.769345538126537	35.050445582483164	20.288882616234524
26	20.125488931005282	21.38602362998508	36.231138352352914	22.25734908665672
27	21.97088592281947	21.59425783297714	35.671599661276666	20.763256582926733
28	20.01693616678092	22.128472922295252	36.21694423162224	21.637646679301582
29	22.198153151336747	22.632364208234204	33.75507076898262	21.41441187144643
30	23.537723295294168	21.225855881285536	35.23400137102303	20.002419452397273
31	24.590507681761363	21.622968668091456	33.0998830598008	20.686640590346386
32	23.079640308076936	24.717125690552038	33.08972135973225	19.113512641638774
33	22.113149723779184	24.896165167950322	31.562401709746364	21.428283398524133
34	21.417476511149644	25.228759224162268	32.599056413565066	20.75470785112303
35	21.612484374369934	27.799346747852738	29.852010161700072	20.73615871607726
36	22.254123150127022	29.55780475019154	28.777450703657404	19.410621396024034
37	22.090245574418326	28.062744465502643	29.47505947820477	20.37195048187427
38	21.942820275011087	28.412435985321988	28.61566998669301	21.029073752973908
39	22.603008185813945	26.174119924190492	29.384410661720228	21.83846122827533
40	22.636719222549296	26.426710754465905	31.45659099157224	19.479979031412554
41	19.99274164280818	27.613371506915602	30.330900439533853	22.06298641074237
42	21.828944715512723	26.177829751199646	31.162546876890197	20.830678656397435
43	22.9783459010444	24.927295455461916	30.20637928948748	21.88797935400621
44	22.226057502318643	24.415823218678174	30.933666680108068	22.424452598895115
45	21.58877374087665	23.7354732045647	30.34686882535586	24.328884229202792
46	22.935602242025887	24.621315375619986	30.82721077462801	21.615871607726117
47	20.739384652606958	24.237267631759345	33.77797491834348	21.245372797290212
48	21.567805153433607	24.17178112020646	31.385942981571834	22.8744707447881
49	21.007621275051413	23.26029275374007	33.669260857292635	22.062825113915885
50	20.7250292350498	23.54304609056817	33.420541150852856	22.311383523529173
51	20.374692527924513	22.4278398322513	32.717448284205005	24.480019355619177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	136.0
1	527.0
2	918.0
3	13725.0
4	26532.0
5	18853.0
6	11174.0
7	10262.5
8	9351.0
9	8985.5
10	8620.0
11	8382.0
12	8144.0
13	7659.0
14	7174.0
15	6655.0
16	6136.0
17	5843.0
18	5550.0
19	5109.0
20	4668.0
21	4389.0
22	4110.0
23	4044.5
24	3979.0
25	4139.0
26	4601.5
27	4904.0
28	5532.0
29	6160.0
30	6853.0
31	7546.0
32	8493.5
33	9441.0
34	10859.0
35	12277.0
36	13288.5
37	14300.0
38	15738.0
39	17176.0
40	18696.0
41	20216.0
42	21681.5
43	23147.0
44	26702.5
45	30258.0
46	44681.0
47	59104.0
48	50956.0
49	42808.0
50	43496.5
51	44185.0
52	40746.0
53	37307.0
54	35488.5
55	33670.0
56	31865.5
57	30061.0
58	28753.5
59	27446.0
60	26381.0
61	25316.0
62	23419.5
63	21523.0
64	19166.0
65	16809.0
66	14337.0
67	11865.0
68	9937.5
69	8010.0
70	6850.5
71	5691.0
72	4814.5
73	3938.0
74	3284.0
75	2067.0
76	1504.0
77	1193.5
78	883.0
79	662.0
80	441.0
81	357.0
82	273.0
83	198.5
84	124.0
85	97.0
86	70.0
87	56.0
88	42.0
89	37.5
90	33.0
91	24.0
92	15.0
93	9.0
94	3.0
95	4.5
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	619975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.157308170373014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28040102393916	32.358652508626
2	8.560617908317605	7.560276862127892
3	4.679495554837349	6.199017818905303
4	3.2239816407578816	5.694494033862823
5	2.417117293329032	5.336669660273398
6	1.8455168853525181	4.8895834704082874
7	1.4061065816857705	4.346291715352184
8	1.0680212905107802	3.7728756206083207
9	0.8420874688361507	3.3465884281026557
>10	2.6030985024789355	15.680440045794539
>50	0.04405883445144092	1.3433977151510899
>100	0.022029416641733923	1.7747094636568292
>500	0.004107179373882585	1.3384514516876733
>1k	0.002613659601561645	2.526537330088927
>5k	3.7337994308023496E-4	1.3457059311006148
>10k+	3.7337994308023496E-4	2.486307944253525
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15080	2.4323561433928784	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	8162	1.3165046977700714	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	4104	0.6619621758941893	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	3027	0.4882454937699101	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	2403	0.3875962740433082	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	1987	0.3204967942255736	No Hit
TAGTGAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT	1465	0.2362998508004355	No Hit
TAGTGAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTAT	1172	0.1890398806403484	No Hit
TAGTGAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGT	1166	0.1880720996814388	No Hit
TAGTGAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	997	0.1608129360054841	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	812	0.1309730231057704	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT	792	0.12774708657607162	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	787	0.1269406024436469	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC	785	0.12661800879067703	No Hit
TAGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	783	0.12629541513770717	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	705	0.11371426267188192	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	639	0.10306867212387595	No Hit
TAGTGAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC	624	0.10064921972660187	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.612968264849389E-4	0.0	0.0	0.09322956570829469	0.0
2	1.612968264849389E-4	0.0	0.0	0.7176095810314932	0.0
3	1.612968264849389E-4	0.0	0.0	0.9776200653252147	0.0
4	1.612968264849389E-4	0.0	0.0	1.7879753215855478	0.0
5	1.612968264849389E-4	0.0	0.0	3.896770031049639	0.0
6	1.612968264849389E-4	0.0	0.0	4.948909230210895	0.0
7	1.612968264849389E-4	0.0	0.0	5.780394370740756	0.0
8	1.612968264849389E-4	0.0	0.0	6.932698899149159	0.0
9	1.612968264849389E-4	0.0	0.0	7.835154643332393	0.0
10	1.612968264849389E-4	0.0	0.0	9.329247147062382	0.0
11	1.612968264849389E-4	0.0	0.0	9.974434453002138	0.0
12	1.612968264849389E-4	0.0	0.0	11.160288721319407	0.0
13	1.612968264849389E-4	0.0	0.0	11.433525545384894	0.0
14	1.612968264849389E-4	0.0	0.0	11.819992741642809	0.0
15	1.612968264849389E-4	0.0	0.0	12.007097060365338	0.0
16	1.612968264849389E-4	0.0	0.0	12.458728174523166	0.0
17	1.612968264849389E-4	0.0	0.0	12.720674220734708	0.0
18	1.612968264849389E-4	0.0	0.0	13.01504092906972	0.0
19	1.612968264849389E-4	0.0	0.0	13.22827533368281	0.0
20	1.612968264849389E-4	0.0	0.0	13.420863744505827	0.0
21	1.612968264849389E-4	0.0	0.0	13.626194604621155	0.0
22	1.612968264849389E-4	0.0	0.0	13.84426791402879	0.0
23	1.612968264849389E-4	0.0	0.0	14.08234202992056	0.0
24	1.612968264849389E-4	0.0	0.0	14.27138191056091	0.0
25	1.612968264849389E-4	0.0	0.0	14.433001330698819	0.0
26	1.612968264849389E-4	0.0	0.0	14.590910923827574	0.0
27	1.612968264849389E-4	0.0	0.0	14.770756885358281	0.0
28	1.612968264849389E-4	0.0	0.0	14.936408726158312	0.0
29	1.612968264849389E-4	0.0	0.0	15.111738376547441	0.0
30	1.612968264849389E-4	0.0	0.0	15.35432880358079	0.0
31	3.225936529698778E-4	0.0	0.0	15.547401104883262	0.0
32	3.225936529698778E-4	0.0	0.0	15.758054760272591	0.0
33	3.225936529698778E-4	0.0	0.0	15.960804871164159	0.0
34	3.225936529698778E-4	0.0	0.0	16.16355498205573	0.0
35	3.225936529698778E-4	0.0	0.0	16.36872454534457	0.0
36	4.838904794548167E-4	0.0	0.0	16.580507278519296	0.0
37	4.838904794548167E-4	0.0	0.0	16.80761321021009	0.0
38	4.838904794548167E-4	0.0	0.0	17.071172224686478	0.0
39	4.838904794548167E-4	0.0	0.0	17.44634864309045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	45	3.8380676E-10	45.000004	2
CGGTCTA	20	7.03077E-4	45.0	31
ACATACG	20	7.03077E-4	45.0	17
CGTTTTT	9390	0.0	43.753994	1
TAGTGAA	925	0.0	42.324326	1
AGTGAAT	890	0.0	41.207867	2
TTAACGG	50	1.0804797E-9	40.500004	2
TCAAGCG	245	0.0	39.489796	17
ACACGAC	245	0.0	38.57143	26
GTTAACG	35	6.243634E-6	38.571426	1
TCGTAAC	35	6.243634E-6	38.571426	34
TCGAAGG	35	6.243634E-6	38.571426	2
ACGGGCT	95	0.0	37.894737	5
CACGACC	250	0.0	37.8	27
CGTTCTG	125	0.0	37.8	1
AGACACG	245	0.0	37.65306	24
ATTACGG	30	1.1393153E-4	37.500004	2
CGTTCAA	30	1.1393153E-4	37.500004	9
TTGGTAG	30	1.1393153E-4	37.500004	1
GCGTAAG	30	1.1393153E-4	37.500004	1
>>END_MODULE