##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533696
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.93919759563497	30.0	25.0	31.0	16.0	33.0
2	28.657241200983332	31.0	28.0	33.0	16.0	34.0
3	28.28668380501259	31.0	27.0	33.0	16.0	34.0
4	32.36208440760283	35.0	32.0	35.0	25.0	37.0
5	33.35833320841827	35.0	32.0	35.0	28.0	37.0
6	33.22834535016189	35.0	33.0	35.0	28.0	37.0
7	34.1553018947116	35.0	33.0	36.0	31.0	37.0
8	34.45956686952872	35.0	35.0	37.0	32.0	37.0
9	35.749199919055044	37.0	35.0	39.0	30.0	39.0
10	35.5302794100012	37.0	35.0	39.0	30.0	39.0
11	35.66166881520566	37.0	35.0	39.0	30.0	39.0
12	35.51675860414918	37.0	35.0	39.0	30.0	39.0
13	35.69667188811608	37.0	35.0	39.0	30.0	39.0
14	36.64860707219091	38.0	35.0	40.0	31.0	41.0
15	36.925931616500776	39.0	35.0	40.0	31.0	41.0
16	36.64275917376184	38.0	35.0	40.0	31.0	41.0
17	35.75911005516249	38.0	34.0	40.0	29.0	41.0
18	35.54365031778391	38.0	33.0	39.0	29.0	41.0
19	35.022108091497785	37.0	33.0	39.0	27.0	40.0
20	35.53403810408922	37.0	34.0	39.0	30.0	40.0
21	35.78799166566735	37.0	34.0	39.0	30.0	40.0
22	36.21266226466003	38.0	35.0	40.0	31.0	41.0
23	36.46742714953832	38.0	35.0	40.0	31.0	41.0
24	36.406519816524764	38.0	35.0	40.0	31.0	41.0
25	35.680537234680415	38.0	34.0	40.0	29.0	41.0
26	35.86918395491066	38.0	34.0	40.0	30.0	41.0
27	36.15083118479434	38.0	35.0	40.0	31.0	41.0
28	36.11826770296199	38.0	35.0	40.0	30.0	41.0
29	36.13411380261422	38.0	35.0	40.0	30.0	41.0
30	35.57201290622377	38.0	34.0	40.0	29.0	41.0
31	35.66546873126274	38.0	34.0	40.0	29.0	41.0
32	35.51845057860655	38.0	34.0	40.0	29.0	41.0
33	35.31463604748771	38.0	34.0	40.0	27.0	41.0
34	35.0832177869049	38.0	34.0	40.0	25.0	41.0
35	34.84209550005996	38.0	34.0	40.0	23.0	41.0
36	34.56210464384219	38.0	34.0	40.0	23.0	41.0
37	34.55739784446576	38.0	34.0	40.0	23.0	41.0
38	34.35942371687253	38.0	34.0	40.0	22.0	41.0
39	34.15834295179278	37.0	33.0	40.0	21.0	41.0
40	34.0578531598513	37.0	33.0	40.0	21.0	41.0
41	33.89394898968701	37.0	33.0	40.0	20.0	41.0
42	33.811471324499344	37.0	33.0	40.0	19.0	41.0
43	33.703479134188754	37.0	33.0	40.0	18.0	41.0
44	33.52180454790742	36.0	33.0	40.0	18.0	41.0
45	33.492563182036214	36.0	33.0	40.0	20.0	41.0
46	33.286929263101094	36.0	33.0	40.0	18.0	40.0
47	33.28193016248951	36.0	33.0	40.0	19.0	41.0
48	33.214091917496106	36.0	33.0	39.0	18.0	40.0
49	33.135213679697806	35.0	33.0	39.0	18.0	40.0
50	32.97175170883799	35.0	32.0	39.0	18.0	40.0
51	30.415909056841347	34.0	27.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	18.0
9	28.0
10	47.0
11	46.0
12	27.0
13	58.0
14	80.0
15	122.0
16	236.0
17	378.0
18	686.0
19	1096.0
20	1669.0
21	2345.0
22	3170.0
23	4545.0
24	6568.0
25	9272.0
26	11864.0
27	12983.0
28	13496.0
29	14067.0
30	16384.0
31	20169.0
32	26311.0
33	36744.0
34	48131.0
35	51912.0
36	66139.0
37	85546.0
38	80503.0
39	19055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.494955929967624	18.947115961146423	22.60294249910061	18.954985609785346
2	37.09358885957549	21.925590598393093	24.424016668665306	16.55680387336611
3	30.881063376903704	21.207391473797816	31.765836731022905	16.14570841827557
4	26.521465403525603	23.313084602470322	32.113787624415394	18.05166236958868
5	24.8928228804413	26.874287984170763	30.113585262021825	18.11930387336611
6	24.758851480992924	31.3708553183835	29.55034326657873	14.319949934044848
7	74.44425290802255	5.297210396930088	16.115728804413	4.142807890634368
8	75.55968191629691	5.1806646480393335	14.90773773833793	4.351915697325818
9	70.32261811967861	5.567589039453172	16.625382240076746	7.484410600791462
10	40.35293500419714	21.78487378582564	22.880253927329417	14.9819372826478
11	31.560851121237558	22.365541431826358	28.778555582204103	17.295051864731985
12	26.885155594195947	21.125322280849023	33.355693128672506	18.633828996282528
13	24.42738937522485	20.959310169085022	35.474127593236595	19.13917286245353
14	21.58588409881281	23.1159686413239	34.97009533517208	20.32805192469121
15	21.052809089818922	21.483203921333494	38.614117400167885	18.8498695886797
16	25.916439321261542	21.639097913418876	32.90768527401367	19.536777491305912
17	24.88776382060199	21.841085561817962	32.87489507135148	20.396255546228563
18	26.51434524523324	21.282902626214177	32.629999100611585	19.572753027941
19	24.92729943638326	24.109230723108286	30.99273743854179	19.97073240196666
20	25.86584872286845	24.324147079985607	31.473348123276175	18.33665607386977
21	25.229906163808607	25.158142463125078	31.549983511212375	18.06196786185394
22	24.386354778750448	21.196898608945915	32.73211716033097	21.684629451972658
23	23.454176160211055	23.587023324139587	32.36617849862094	20.592622017028418
24	24.030721609305672	21.71104898668905	32.611636587120756	21.646592816884517
25	23.336131430627173	23.469165967142345	31.24081874325459	21.953883858975896
26	22.27429098213215	22.38502818083703	32.00042720949754	23.34025362753328
27	22.92597283846984	22.21601810768677	31.82354748770836	23.03446156613503
28	20.7307156133829	23.711063976495982	32.557111164408205	23.001109245712914
29	23.910428408682098	24.150452692169324	30.360354958628132	21.578763940520446
30	25.15739297277851	22.363292960786666	32.090178678498624	20.389135387936204
31	25.794085022184916	22.301272634608466	29.676819762561458	22.227822580645164
32	26.868292061398247	23.03333733061518	30.154057740736302	19.944312867250268
33	24.721002218491424	22.74965523444058	30.06224517328217	22.467097373785823
34	22.801932186113444	24.717629511931886	31.165307590838232	21.31513071111644
35	23.255561218371508	25.43432965583403	30.376656373665906	20.933452752128552
36	23.28760193068713	28.617602530279413	27.660878102890035	20.433917436143425
37	24.85853369708598	25.86528660510853	27.645700923372107	21.630478774433385
38	23.60969540712316	26.205929967622016	28.091835052164527	22.0925395730903
39	24.73674151576928	24.466175500659553	27.311615601391054	23.48546738218012
40	24.540937162729342	22.934966722628612	30.208770536035495	22.315325578606547
41	21.179660330974936	26.19225176879722	27.75362753327737	24.874460366950473
42	22.721736719031057	24.404529919654635	29.60786665067754	23.265866710636768
43	23.291162009833315	23.953149358436264	29.024950533637128	23.730738098093298
44	23.273923731862332	23.549174061638087	28.980168485429907	24.19673372106967
45	22.935528840388535	22.99361434224727	28.88273474037654	25.188122076987646
46	23.7243674301475	24.04870937762322	28.739207339009475	23.48771585321981
47	21.51168455450294	23.77833073510013	31.856899808130475	22.85308490226646
48	22.40189171363473	24.133027041611705	30.00059959227725	23.464481652476316
49	23.203096894112004	22.684074829116202	31.385845125314788	22.72698315145701
50	21.558902446336493	23.273361614102413	30.928468641323896	24.2392672982372
51	21.302202002638207	22.432808190430507	29.709422592637008	26.55556721429428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	536.0
2	952.0
3	7935.0
4	14918.0
5	10217.5
6	5517.0
7	4936.5
8	4356.0
9	4289.0
10	4222.0
11	4044.0
12	3866.0
13	3646.5
14	3427.0
15	3243.0
16	3059.0
17	2849.5
18	2640.0
19	2453.5
20	2267.0
21	2277.0
22	2287.0
23	2369.5
24	2452.0
25	2639.0
26	3417.5
27	4009.0
28	4371.0
29	4733.0
30	5670.0
31	6607.0
32	7477.5
33	8348.0
34	9624.5
35	10901.0
36	11460.5
37	12020.0
38	13134.0
39	14248.0
40	15335.5
41	16423.0
42	18182.5
43	19942.0
44	22622.5
45	25303.0
46	33157.0
47	41011.0
48	39701.0
49	38391.0
50	39690.0
51	40989.0
52	39385.5
53	37782.0
54	35438.0
55	33094.0
56	31816.5
57	30539.0
58	29579.0
59	28619.0
60	27194.0
61	25769.0
62	23943.5
63	22118.0
64	19701.0
65	17284.0
66	15019.0
67	12754.0
68	11164.5
69	9575.0
70	8112.0
71	6649.0
72	5669.0
73	4689.0
74	4086.5
75	2865.0
76	2246.0
77	1828.0
78	1410.0
79	1169.5
80	929.0
81	671.5
82	414.0
83	326.5
84	239.0
85	182.5
86	126.0
87	95.5
88	65.0
89	51.5
90	38.0
91	28.5
92	19.0
93	14.0
94	9.0
95	7.5
96	6.0
97	5.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.08362029702314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66774743342168	29.18285889121877
2	7.281568473814113	5.691801147946502
3	3.2222270794186687	3.7780889904835515
4	2.1838265581876413	3.4140739197904266
5	1.7691324943039006	3.4572051331249547
6	1.4525633462912941	3.406286057029336
7	1.287880571820738	3.5234524679868326
8	1.109121785705119	3.4678795788524113
9	1.0242602405502197	3.6028618494302127
>10	5.939717536016281	34.6778466797907
>50	0.04146559716525796	1.1510255387295687
>100	0.017561899975797483	1.2621813924739445
>500	4.878305548832635E-4	0.11725703268451297
>1k	0.001951322219533054	1.732734817946104
>5k	4.878305548832635E-4	1.5344465025121308
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8048	1.5079745772874444	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	2853	0.5345739896870128	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	2647	0.49597523683894956	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	2380	0.44594675620578006	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1208	0.22634608466242956	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	615	0.1152341407842667	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8737258664108407E-4	0.0	0.0	0.10511602110564816	0.0
2	1.8737258664108407E-4	0.0	0.0	0.566052584242715	0.0
3	1.8737258664108407E-4	0.0	0.0	0.7760972538673702	0.0
4	1.8737258664108407E-4	0.0	0.0	1.1107446936083463	0.0
5	1.8737258664108407E-4	0.0	0.0	2.3037459527521285	0.0
6	1.8737258664108407E-4	0.0	0.0	2.873358616141024	0.0
7	1.8737258664108407E-4	0.0	0.0	3.4733256385657754	0.0
8	1.8737258664108407E-4	0.0	0.0	4.374962525482672	0.0
9	1.8737258664108407E-4	0.0	0.0	4.852200503657513	0.0
10	1.8737258664108407E-4	0.0	0.0	6.080053063916536	0.0
11	1.8737258664108407E-4	0.0	0.0	6.755531238757645	0.0
12	1.8737258664108407E-4	0.0	0.0	7.816434824319463	0.0
13	1.8737258664108407E-4	0.0	0.0	8.092809389615061	0.0
14	1.8737258664108407E-4	0.0	0.0	8.23952212495503	0.0
15	1.8737258664108407E-4	0.0	0.0	8.47111464204341	0.0
16	1.8737258664108407E-4	0.0	0.0	8.79414498141264	0.0
17	1.8737258664108407E-4	0.0	0.0	9.16739117400168	0.0
18	1.8737258664108407E-4	0.0	0.0	9.579236119438782	0.0
19	1.8737258664108407E-4	0.0	0.0	9.8278795419115	0.0
20	1.8737258664108407E-4	0.0	0.0	10.081582024223527	0.0
21	3.7474517328216813E-4	0.0	0.0	10.366200983331336	0.0
22	3.7474517328216813E-4	0.0	0.0	10.674054143182635	0.0
23	3.7474517328216813E-4	0.0	0.0	10.980783067514091	0.0
24	3.7474517328216813E-4	0.0	0.0	11.228489627053603	0.0
25	3.7474517328216813E-4	0.0	0.0	11.437784806331694	0.0
26	3.7474517328216813E-4	0.0	0.0	11.660945557021225	0.0
27	3.7474517328216813E-4	0.0	0.0	11.90715313586761	0.0
28	3.7474517328216813E-4	0.0	0.0	12.133873965703321	0.0
29	3.7474517328216813E-4	0.0	0.0	12.36340538433865	0.0
30	3.7474517328216813E-4	0.0	0.0	12.649523324139585	0.0
31	3.7474517328216813E-4	0.0	0.0	12.895356157812687	0.0
32	3.7474517328216813E-4	0.0	0.0	13.16554742774913	0.0
33	3.7474517328216813E-4	0.0	0.0	13.418125674541312	0.0
34	3.7474517328216813E-4	0.0	0.0	13.666769097014031	0.0
35	3.7474517328216813E-4	0.0	0.0	13.925343266578727	0.0
36	3.7474517328216813E-4	0.0	0.0	14.199281988247991	0.0
37	3.7474517328216813E-4	0.0	0.0	14.476031298716872	0.0
38	3.7474517328216813E-4	0.0	0.0	14.810491365871208	0.0
39	3.7474517328216813E-4	0.0	0.0	15.292413658712075	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	25	3.8879876E-5	45.0	2
CGTAAGG	40	6.8030204E-9	45.0	2
TACGTTG	25	3.8879876E-5	45.0	1
CGTTTTT	4645	0.0	43.595264	1
TACGGGA	190	0.0	40.26316	4
AGACACG	285	0.0	40.263157	24
ACGTTGG	45	1.9250365E-8	40.0	2
GCGTTAG	45	1.9250365E-8	40.0	1
TCGTACG	45	1.9250365E-8	40.0	30
CACGACC	295	0.0	38.898304	27
ACGACCA	295	0.0	38.898304	28
TCGACGG	35	6.2420004E-6	38.571426	2
TGTCGTG	35	6.2420004E-6	38.571426	27
GCGAGAC	300	0.0	38.250004	21
GACACGA	300	0.0	38.250004	25
CGACGGG	100	0.0	38.25	3
GCGATAT	65	9.094947E-12	38.07692	9
TGCTAGG	160	0.0	37.96875	2
CGAGACA	305	0.0	37.62295	22
CGCATCG	30	1.1391027E-4	37.499996	21
>>END_MODULE