FastQCFastQC Report
Sat 18 Jun 2016
SRR3552268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408540
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111172.721153375434474No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG76581.8744798550937483No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC66131.618690948254761No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC63851.5628824594898907No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC25650.6278454986047878TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT16030.3923728398688011No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT13300.3255495177950751No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT12080.2956870808243991TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC11900.2912811475008567No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA11190.2739021882802174No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC8660.21197434767709403TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC7810.19116855142703285No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC5110.12507955157389727TruSeq Adapter, Index 20 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT4150.10158124051500465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA302.1620526E-645.00000410
TCTACGG253.8863767E-545.02
CGAGATT207.0279423E-445.022
TAGTCCG207.0279423E-445.01
CGTTTTT60950.044.04021
CGGGTAC603.6379788E-1241.2500046
TACGGCT7900.040.7278487
CACGACG451.9230356E-840.026
TATGCGG451.9230356E-840.02
CGGTCTA451.9230356E-840.031
TGACGGG1200.039.3750043
TTTAGCG403.450932E-739.3751
CACGGGT403.450932E-739.3754
ACGGGTA403.450932E-739.3755
CCGACTT403.450932E-739.37512
GCGAGAC1150.039.13043621
GTTTTTT70400.039.119322
CACGTGA1100.038.86363643
TGGGTAC1450.038.7931026
TTGATAG356.238406E-638.5714261
Produced by FastQC (version 0.11.5)