##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408540
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.810848386938854	28.0	16.0	30.0	16.0	33.0
2	26.088725706173204	28.0	25.0	31.0	16.0	33.0
3	27.039418416801293	30.0	25.0	31.0	16.0	33.0
4	28.240093993244237	32.0	22.0	35.0	19.0	35.0
5	30.009742008126498	32.0	28.0	35.0	19.0	35.0
6	30.472411514172418	33.0	28.0	35.0	17.0	35.0
7	31.966566309296518	33.0	31.0	35.0	28.0	35.0
8	33.21373672100651	35.0	33.0	35.0	28.0	37.0
9	33.97536593723993	35.0	32.0	37.0	27.0	39.0
10	34.13994957654085	35.0	32.0	39.0	27.0	39.0
11	34.58730356880599	37.0	33.0	39.0	27.0	39.0
12	32.56977284965976	35.0	30.0	37.0	23.0	39.0
13	33.694438733049395	35.0	32.0	37.0	27.0	39.0
14	34.796548685563224	37.0	33.0	39.0	27.0	40.0
15	35.28111323248641	37.0	33.0	39.0	27.0	40.0
16	35.714475938708574	37.0	34.0	39.0	30.0	41.0
17	33.838796690654526	36.0	31.0	39.0	24.0	41.0
18	34.127551769716554	36.0	32.0	39.0	26.0	40.0
19	33.94051990013218	37.0	32.0	39.0	25.0	40.0
20	34.294404464679104	35.0	32.0	39.0	27.0	40.0
21	35.08152690067068	36.0	34.0	39.0	30.0	40.0
22	35.80370832721398	37.0	34.0	39.0	30.0	40.0
23	36.219733685808	38.0	35.0	40.0	31.0	40.0
24	35.97264649728301	38.0	35.0	40.0	31.0	41.0
25	34.82202477113624	36.0	33.0	39.0	27.0	40.0
26	35.126920252606844	37.0	34.0	39.0	27.0	40.0
27	35.78732804621335	37.0	35.0	40.0	30.0	40.0
28	35.686625544622316	38.0	35.0	40.0	30.0	40.0
29	35.82623243746023	38.0	35.0	40.0	30.0	41.0
30	35.36698242522152	37.0	34.0	40.0	29.0	41.0
31	35.122357663876244	37.0	34.0	40.0	27.0	41.0
32	34.75480246732266	37.0	34.0	40.0	25.0	40.0
33	34.61516375385519	38.0	34.0	40.0	23.0	41.0
34	33.20387232584325	36.0	32.0	39.0	19.0	40.0
35	33.231989523669654	36.0	33.0	39.0	18.0	40.0
36	33.007524355020315	36.0	32.0	39.0	16.0	40.0
37	32.93443971214569	36.0	32.0	39.0	15.0	40.0
38	33.03907328535762	37.0	33.0	39.0	15.0	40.0
39	32.514732951485776	36.0	32.0	39.0	15.0	40.0
40	32.61773388162726	36.0	32.0	39.0	15.0	40.0
41	32.44763793019043	36.0	31.0	39.0	13.0	40.0
42	32.58667449943702	36.0	32.0	39.0	13.0	40.0
43	32.62917706956479	36.0	32.0	39.0	12.0	40.0
44	32.230268761932734	36.0	31.0	39.0	12.0	40.0
45	32.416323982963725	36.0	31.0	39.0	12.0	40.0
46	32.08410681940569	35.0	31.0	39.0	11.0	40.0
47	32.22310912028198	36.0	31.0	39.0	12.0	40.0
48	32.261350173789594	36.0	31.0	39.0	12.0	40.0
49	31.903218779066922	35.0	31.0	38.0	12.0	40.0
50	31.89417682479072	35.0	31.0	38.0	12.0	40.0
51	28.927847946345523	33.0	24.0	36.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	13.0
10	33.0
11	32.0
12	37.0
13	30.0
14	61.0
15	104.0
16	182.0
17	372.0
18	700.0
19	1050.0
20	1578.0
21	2304.0
22	3343.0
23	4732.0
24	7145.0
25	10100.0
26	12600.0
27	13257.0
28	12975.0
29	13664.0
30	16668.0
31	21247.0
32	28475.0
33	38079.0
34	52028.0
35	65541.0
36	58473.0
37	34041.0
38	9195.0
39	470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.772262201987566	15.898565623929114	19.08650315758555	24.24266901649777
2	40.6043471875459	25.044548881382482	20.121163166397416	14.229940764674206
3	27.154501395212222	23.71738385470211	35.116757233073876	14.011357517011799
4	23.359279385127525	20.21833847358888	40.14123464042689	16.28114750085671
5	24.951045185293975	23.184755470700544	33.501248347775004	18.362950996230477
6	20.48098105448671	32.07568414353552	34.50800411220444	12.93533068977334
7	63.08562197092083	4.808586674499437	27.673911979243158	4.431879375336564
8	61.50242326332794	9.077446516864935	23.58594017721643	5.834190042590689
9	55.85964654623782	6.218240563959466	24.938316933470407	12.983795956332306
10	31.27869976012141	20.086650022029666	32.31898957262447	16.315660645224458
11	24.714838204337397	20.46531551378078	36.196455671415286	18.62339061046654
12	22.106525676800313	17.480540461154355	41.02217653106183	19.3907573309835
13	20.361776080677537	18.71640475840799	44.62108973417536	16.300729426739117
14	18.243501248347773	24.191511235129976	37.60390659421354	19.961080922308707
15	15.455769324913105	20.604347187545898	46.380281000636415	17.559602486904588
16	17.484456846330836	21.032212268076567	39.142066872276885	22.34126401331571
17	16.945464336417487	20.15053605522103	40.60630538013414	22.297694228227346
18	18.16223625593577	20.89073285357615	40.35149556958927	20.59553532089881
19	18.732315073187447	22.91452489352328	37.32437460224213	21.028785431047144
20	19.88887257061732	21.966759680814608	40.164732951485774	17.97963479708229
21	19.653644685954863	24.87100406324962	37.48421207225731	17.99113917853821
22	17.134429921182747	20.46433641748666	37.37357419102169	25.027659470308905
23	17.85822685661135	25.473882606354337	37.232094776521265	19.43579576051305
24	20.123855681206244	20.813629020414158	36.19816908992999	22.8643462084496
25	17.609781171978263	26.059382190238413	35.06462035541196	21.26621628237137
26	16.84412786997601	22.681010427375533	37.50966857590444	22.965193126744015
27	19.5133891418221	23.878934743231998	35.274636510500805	21.333039604445098
28	15.955353208988104	23.8253292211289	39.096538894600286	21.122778675282714
29	20.57252655798698	24.717775493219758	33.69829147696676	21.011406471826504
30	19.95838840749988	25.73285357614921	36.039555490282474	18.26920252606844
31	20.83786165369364	25.293484114162627	32.71968473099329	21.14896950115044
32	22.86140891956724	26.348949919224555	31.152641112253388	19.637000048954814
33	20.91423116463504	23.51201840701033	30.92622509423802	24.64752533411661
34	20.563225143192835	25.52430606550154	33.209232878053555	20.70323591325207
35	19.503353404807363	25.334606158515687	29.34498457923337	25.817055857443577
36	19.746414059822783	29.384393205071717	28.63954569931953	22.22964703578597
37	21.19180496401821	26.920986929064473	31.86224115141724	20.024966955500073
38	22.166250550741665	26.928574925343906	27.97694228227346	22.928232241640966
39	24.187350075879962	21.538160278063348	31.986341606697017	22.28814803935967
40	22.54393694619866	21.819895236696528	33.13824839672982	22.49791942037499
41	17.582366475742887	24.956430214911638	31.906545258726194	25.554658050619278
42	19.26959416458609	24.482792382630834	31.898222940226173	24.34939051255691
43	21.776080677534633	23.188427081803496	29.671023645175502	25.36446859548637
44	21.74205708131395	22.91917560092035	31.78856415528467	23.55020316248103
45	21.11714887159152	21.215548049150634	31.651735448181327	26.015567631076514
46	24.215009546188867	23.279238263083172	30.678513731825525	21.827238458902436
47	18.68336025848142	22.375042835462867	37.53267733881627	21.408919567239437
48	20.326528614089195	23.425123610907132	31.453468448621923	24.79487932638175
49	19.729769422822734	20.600186028295884	35.80163509081118	23.8684094580702
50	20.641797620796005	19.57066627502815	34.570911049101674	25.216625055074164
51	20.211484799530034	20.01860282958829	31.37342732657757	28.39648504430411
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	469.5
2	732.0
3	9173.5
4	17615.0
5	12180.0
6	6745.0
7	6425.5
8	6106.0
9	5919.0
10	5732.0
11	5500.5
12	5269.0
13	5039.0
14	4809.0
15	4412.5
16	4016.0
17	3743.5
18	3471.0
19	3214.5
20	2958.0
21	2859.5
22	2761.0
23	2580.0
24	2399.0
25	2446.0
26	2591.5
27	2690.0
28	3062.0
29	3434.0
30	3958.0
31	4482.0
32	4708.0
33	4934.0
34	5760.5
35	6587.0
36	7110.5
37	7634.0
38	8314.5
39	8995.0
40	10478.5
41	11962.0
42	13909.5
43	15857.0
44	18233.0
45	20609.0
46	37688.0
47	54767.0
48	46562.0
49	38357.0
50	37003.0
51	35649.0
52	30843.5
53	26038.0
54	23064.5
55	20091.0
56	18045.0
57	15999.0
58	15060.5
59	14122.0
60	13423.5
61	12725.0
62	11945.0
63	11165.0
64	9735.0
65	8305.0
66	7182.5
67	6060.0
68	5267.0
69	4474.0
70	3766.0
71	3058.0
72	2665.5
73	2273.0
74	1806.0
75	1039.5
76	740.0
77	564.5
78	389.0
79	301.5
80	214.0
81	165.0
82	116.0
83	98.0
84	80.0
85	58.0
86	36.0
87	32.0
88	28.0
89	19.0
90	10.0
91	7.0
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	408540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.70278696704882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.07907644160159	35.68431397194438
2	7.8528962286466175	7.17798486824355
3	3.437159681543274	4.712633300919049
4	2.211879157922088	4.043561678054742
5	1.727367391640486	3.9472751956985954
6	1.3269308306909868	3.6386662245047585
7	1.1055055166911303	3.5367278184162383
8	0.9209623373189992	3.3672436405732467
9	0.7294992544926582	3.000613411862901
>10	2.527219414311394	16.53130418871015
>50	0.04455538067696434	1.4040571796604107
>100	0.029884706551622328	2.2908432052294407
>500	0.0016300749028157634	0.5358958955973044
>1k	0.0032601498056315267	2.2386939290128356
>5k	0.0016300749028157634	5.129502140619981
>10k+	5.433583009385878E-4	2.760683350952379
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11117	2.721153375434474	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCG	7658	1.8744798550937483	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGC	6613	1.618690948254761	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTC	6385	1.5628824594898907	No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	2565	0.6278454986047878	TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT	1603	0.3923728398688011	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCT	1330	0.3255495177950751	No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	1208	0.2956870808243991	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTC	1190	0.2912811475008567	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTA	1119	0.2739021882802174	No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	866	0.21197434767709403	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCC	781	0.19116855142703285	No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC	511	0.12507955157389727	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT	415	0.10158124051500465	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3395016399862926	0.0
2	0.0	0.0	0.0	1.527879766975082	0.0
3	0.0	0.0	0.0	1.9031184216967738	0.0
4	0.0	0.0	0.0	2.7818573456699465	0.0
5	0.0	0.0	0.0	5.72526557986978	0.0
6	0.0	0.0	0.0	6.682821755519655	0.0
7	0.0	0.0	0.0	7.861408919567239	0.0
8	0.0	0.0	0.0	9.40495422724825	0.0
9	0.0	0.0	0.0	10.024477407353013	0.0
10	0.0	0.0	0.0	13.185245018847604	0.0
11	0.0	0.0	0.0	14.739560385763939	0.0
12	0.0	0.0	0.0	17.7701081901405	0.0
13	0.0	0.0	0.0	18.4070103294659	0.0
14	0.0	0.0	0.0	18.702207862143243	0.0
15	0.0	0.0	0.0	19.373623145836394	0.0
16	0.0	0.0	0.0	20.18382532922113	0.0
17	0.0	0.0	0.0	21.073089538356097	0.0
18	0.0	0.0	0.0	22.084985558329663	0.0
19	0.0	0.0	0.0	22.683702942184365	0.0
20	0.0	0.0	0.0	23.219513389141824	0.0
21	0.0	0.0	0.0	23.788613110099377	0.0
22	0.0	0.0	0.0	24.407646742057082	0.0
23	0.0	0.0	0.0	24.988740392617615	0.0
24	0.0	0.0	0.0	25.438390365692467	0.0
25	0.0	0.0	0.0	25.831497527781856	0.0
26	0.0	0.0	0.0	26.187399030694667	0.0
27	0.0	0.0	0.0	26.566554070592844	0.0
28	0.0	0.0	0.0	26.956234395652814	0.0
29	0.0	0.0	0.0	27.314338865227395	0.0
30	0.0	0.0	0.0	27.778430508640525	0.0
31	0.0	0.0	0.0	28.16297057815636	0.0
32	0.0	0.0	0.0	28.578352180936996	0.0
33	0.0	0.0	0.0	28.95750722083517	0.0
34	0.0	0.0	0.0	29.33568316443922	0.0
35	0.0	0.0	0.0	29.714348656190335	0.0
36	0.0	0.0	0.0	30.05507416654428	0.0
37	0.0	0.0	0.0	30.399960836148235	0.0
38	0.0	0.0	0.0	30.738238605766877	0.0
39	2.447740735301317E-4	0.0	0.0	31.099525138297352	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	30	2.1620526E-6	45.000004	10
TCTACGG	25	3.8863767E-5	45.0	2
CGAGATT	20	7.0279423E-4	45.0	22
TAGTCCG	20	7.0279423E-4	45.0	1
CGTTTTT	6095	0.0	44.0402	1
CGGGTAC	60	3.6379788E-12	41.250004	6
TACGGCT	790	0.0	40.727848	7
CACGACG	45	1.9230356E-8	40.0	26
TATGCGG	45	1.9230356E-8	40.0	2
CGGTCTA	45	1.9230356E-8	40.0	31
TGACGGG	120	0.0	39.375004	3
TTTAGCG	40	3.450932E-7	39.375	1
CACGGGT	40	3.450932E-7	39.375	4
ACGGGTA	40	3.450932E-7	39.375	5
CCGACTT	40	3.450932E-7	39.375	12
GCGAGAC	115	0.0	39.130436	21
GTTTTTT	7040	0.0	39.11932	2
CACGTGA	110	0.0	38.863636	43
TGGGTAC	145	0.0	38.793102	6
TTGATAG	35	6.238406E-6	38.571426	1
>>END_MODULE