##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552267_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495387
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.978856934073765	26.0	16.0	30.0	16.0	33.0
2	24.783631786865623	28.0	16.0	31.0	16.0	33.0
3	25.991150353158236	30.0	16.0	31.0	16.0	33.0
4	26.98958188244746	28.0	19.0	35.0	19.0	35.0
5	29.578164142377574	32.0	28.0	35.0	19.0	35.0
6	28.78814946698238	33.0	25.0	35.0	15.0	35.0
7	30.40624804445817	32.0	28.0	35.0	23.0	35.0
8	32.09270731771322	35.0	32.0	35.0	26.0	37.0
9	32.81346906559922	35.0	31.0	37.0	25.0	39.0
10	31.875166284137453	35.0	28.0	37.0	17.0	39.0
11	32.685326825290126	35.0	31.0	39.0	18.0	39.0
12	32.9049046503037	35.0	31.0	39.0	23.0	39.0
13	33.27573392115659	35.0	32.0	39.0	25.0	39.0
14	32.94804264140964	36.0	31.0	39.0	19.0	41.0
15	33.754008482257305	36.0	32.0	39.0	25.0	41.0
16	33.927533423363954	36.0	32.0	39.0	24.0	41.0
17	31.91428519521102	34.0	27.0	38.0	18.0	41.0
18	32.641464148231584	35.0	30.0	39.0	18.0	39.0
19	32.56722925712625	36.0	30.0	37.0	18.0	40.0
20	32.68533691840924	34.0	30.0	37.0	25.0	39.0
21	33.343448657312365	35.0	32.0	38.0	25.0	39.0
22	33.95950438747888	35.0	32.0	38.0	27.0	40.0
23	34.9659518719708	36.0	33.0	39.0	29.0	40.0
24	32.86575747849661	35.0	31.0	37.0	24.0	39.0
25	32.07212542920989	35.0	30.0	38.0	19.0	39.0
26	31.875892988713876	34.0	29.0	37.0	21.0	39.0
27	33.22574875804169	35.0	31.0	38.0	24.0	39.0
28	33.539576129369564	35.0	32.0	38.0	24.0	40.0
29	33.75968687107252	35.0	32.0	39.0	24.0	40.0
30	32.29096847515175	35.0	30.0	38.0	20.0	40.0
31	32.499165299049025	35.0	30.0	38.0	22.0	40.0
32	32.57671073322473	35.0	31.0	38.0	21.0	40.0
33	32.910209593711585	35.0	31.0	38.0	20.0	40.0
34	32.23317123784032	35.0	31.0	38.0	16.0	40.0
35	32.115852858472266	35.0	31.0	38.0	15.0	40.0
36	31.554501833919744	35.0	30.0	38.0	12.0	40.0
37	31.776033686794364	35.0	31.0	38.0	10.0	40.0
38	31.432427576823777	35.0	30.0	39.0	10.0	40.0
39	31.116272732227532	35.0	30.0	38.0	10.0	40.0
40	30.95386838976396	35.0	29.0	38.0	10.0	40.0
41	30.58596208620735	35.0	27.0	38.0	10.0	40.0
42	31.490483197984606	35.0	30.0	39.0	10.0	40.0
43	31.53940050909693	35.0	30.0	39.0	10.0	40.0
44	31.679410238863756	35.0	31.0	39.0	10.0	40.0
45	31.957978307868395	35.0	31.0	39.0	10.0	40.0
46	31.784610819419967	35.0	31.0	39.0	10.0	40.0
47	31.733779852923067	35.0	31.0	39.0	10.0	40.0
48	31.612412114165288	35.0	31.0	38.0	10.0	40.0
49	31.69819757078809	35.0	31.0	38.0	10.0	40.0
50	31.76793294939108	35.0	31.0	39.0	10.0	40.0
51	30.25671646611639	34.0	27.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	26.0
9	50.0
10	63.0
11	67.0
12	52.0
13	78.0
14	107.0
15	168.0
16	308.0
17	569.0
18	1118.0
19	1779.0
20	2761.0
21	4125.0
22	6053.0
23	8625.0
24	12071.0
25	16470.0
26	19720.0
27	21125.0
28	22111.0
29	25053.0
30	31161.0
31	38818.0
32	48843.0
33	60823.0
34	70341.0
35	61656.0
36	31737.0
37	8702.0
38	797.0
39	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.34537442443988	15.181867913368741	18.62786064228573	27.844897019905652
2	43.197944233498255	22.11079418717084	18.17831311681574	16.512948462515165
3	25.01095103424192	21.095224541620997	38.25776615050456	15.636058273632534
4	22.23816935042704	18.82790626318414	43.32208959863703	15.6118347877518
5	24.250333577586815	21.36410523489716	37.16851673540081	17.217044452115214
6	19.89535454099522	27.393532732994608	38.06740992395844	14.643702802051727
7	58.69895657334568	4.7235797467434555	32.757016231754164	3.8204474481566932
8	57.836600475991496	9.122362920302713	27.093565232838163	5.947471370867625
9	52.133180725372284	5.634382815859116	28.0738089614786	14.158627497289997
10	29.846968127948454	19.560061123929373	35.20500134238485	15.387969405737334
11	23.384343957350513	20.010012374164038	39.59974726829731	17.005896400188135
12	20.44623698239962	16.910617355723907	44.448683554473575	18.1944621074029
13	18.496650093765076	18.78208350239308	47.79535999127955	14.9259064125623
14	17.243892148966363	22.011074170295142	41.287720509419906	19.457313171318585
15	15.005843915968727	20.114375225833538	48.186973013018104	16.692807845179626
16	16.774562110027112	20.44623698239962	40.79820423224671	21.980996675326566
17	16.977030079513593	19.942186613697977	42.92664119163401	20.154142115154414
18	17.789728030812274	19.898382476730315	42.46478006084132	19.84710943161609
19	17.662352867556073	22.21132165357589	40.22794300213772	19.898382476730315
20	18.624428981785957	20.87458895772396	44.19110715460842	16.309874905881664
21	19.34931679676697	22.805806369565612	40.916495588297636	16.92838124536978
22	16.85268285199248	18.74029798924881	41.73928665871329	22.667732500045418
23	17.375910147016373	23.328024352677808	41.41267332408803	17.883392176217786
24	19.239705523156644	19.64020048971814	39.99781988627073	21.122274100854483
25	17.2220910116737	23.56077167951521	38.91664496646057	20.300492342350527
26	16.998023767276894	19.19953490907109	41.236649326688024	22.56579199696399
27	20.025757639986516	20.362060368964062	39.12779301838765	20.484388972661776
28	15.824194013972914	21.042942184595073	42.60568000371427	20.527183797717743
29	19.851954128792237	19.396754456616748	37.81972478082792	22.931566633763097
30	20.011021686075733	21.06312842282902	40.48390450294416	18.441945388151083
31	19.802901569883748	22.9085543221764	36.05060286200485	21.237941245934998
32	19.27139791718394	22.903305900235573	37.2779261466288	20.54737003595169
33	18.18921368546207	21.78700692589834	37.09806676396433	22.925712624675253
34	18.564273991848797	24.396280080018247	35.977730541980314	21.061715386152642
35	20.174933940535382	21.85341964968802	36.202807098288815	21.76883931148779
36	21.61663507520383	25.008326823271503	33.21463825251773	20.16039984900694
37	20.569171173244353	24.022229085543223	34.86930420055431	20.539295540658113
38	19.395139557558032	25.97726625850093	34.90463011746372	19.72296406647732
39	21.393980867483403	24.29050419167237	33.35452888347898	20.960986057365254
40	20.13678195027322	22.466071980088294	39.48448384798148	17.91266222165701
41	21.010846065803097	26.517853718406016	33.098769245054875	19.37253097073601
42	19.293199054476602	23.34114540752987	37.57950854584395	19.786146992149572
43	23.22628571197872	21.723420275461407	32.84502823045417	22.205265782105705
44	21.233298411141188	22.532484703877977	34.03944794675678	22.194768938224055
45	19.077206305373377	22.03004923423505	34.44963230767057	24.443112152721003
46	22.53551263961307	23.366580067704643	34.18317396298248	19.91473332969981
47	18.40601388409466	21.408716821394183	40.65023910599188	19.53503018851928
48	19.663010938922497	23.346393829470696	34.52472511390085	22.465870117705954
49	17.948795588095773	21.216140108642335	39.28423636470073	21.550827938561167
50	19.819454285235583	19.829345541970216	37.500984079113906	22.850216093680295
51	19.352546594884405	20.242154113854422	35.06369767474722	25.34160161651396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	508.5
2	885.0
3	16947.5
4	33010.0
5	22256.5
6	11503.0
7	10547.0
8	9591.0
9	9041.5
10	8492.0
11	7917.5
12	7343.0
13	6828.5
14	6314.0
15	5783.0
16	5252.0
17	4813.0
18	4374.0
19	4089.0
20	3804.0
21	3798.0
22	3792.0
23	3677.0
24	3562.0
25	3547.5
26	3731.0
27	3929.0
28	4419.5
29	4910.0
30	5525.5
31	6141.0
32	6925.5
33	7710.0
34	8491.0
35	9272.0
36	9957.5
37	10643.0
38	11384.0
39	12125.0
40	12871.5
41	13618.0
42	15329.5
43	17041.0
44	19628.5
45	22216.0
46	42363.0
47	62510.0
48	50814.5
49	39119.0
50	37841.5
51	36564.0
52	31842.5
53	27121.0
54	24635.5
55	22150.0
56	20735.5
57	19321.0
58	17990.0
59	16659.0
60	15953.5
61	15248.0
62	14273.0
63	13298.0
64	11864.0
65	10430.0
66	9068.0
67	7706.0
68	6573.0
69	5440.0
70	4583.0
71	3726.0
72	3276.0
73	2826.0
74	2382.0
75	1505.5
76	1073.0
77	813.0
78	553.0
79	413.5
80	274.0
81	199.0
82	124.0
83	90.5
84	57.0
85	45.5
86	34.0
87	25.0
88	16.0
89	10.5
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	495387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.04426896340387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25916134918616	37.118599277473166
2	8.813223416451528	8.468497525091328
3	4.319578274222223	6.225929412456252
4	2.7299572949021313	5.246352101395378
5	1.9511601495443287	4.687103150769152
6	1.41784250473906	4.087152398725777
7	0.9984934442787486	3.35803213165866
8	0.6810985447380415	2.6178305339181946
9	0.48449560651362333	2.094951350783521
>10	1.262677940926028	9.077407303943374
>50	0.046728910003641995	1.5268442664291628
>100	0.030438097338683905	2.919809027273829
>500	8.574111926389833E-4	0.22718322214186185
>1k	0.00257223357791695	2.379141794161964
>5k	8.574111926389833E-4	3.5840573422398347
>10k+	8.574111926389833E-4	6.381109161538551
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20749	4.188442571161537	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	10232	2.0654558960973946	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	9568	1.9314192742239906	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC	7833	1.5811880408650207	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	3678	0.7424498422445482	RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	2824	0.570059367726646	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGACTATGT	1597	0.3223742245961238	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA	1307	0.26383413371767933	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	1128	0.22770076727891528	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC	1017	0.20529404283923477	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	596	0.12030997987432049	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	507	0.1023442278461082	RNA PCR Primer, Index 38 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.111831759816063	0.0
2	0.0	0.0	0.0	1.084808442692279	0.0
3	2.0186238233946388E-4	0.0	0.0	1.3444034663808295	0.0
4	2.0186238233946388E-4	0.0	0.0	2.271153663701308	0.0
5	2.0186238233946388E-4	0.0	0.0	5.001140522460218	0.0
6	2.0186238233946388E-4	0.0	0.0	5.745407126145821	0.0
7	2.0186238233946388E-4	0.0	0.0	6.577282003766752	0.0
8	2.0186238233946388E-4	0.0	0.0	7.55328662237806	0.0
9	2.0186238233946388E-4	0.0	0.0	8.032306055669608	0.0
10	2.0186238233946388E-4	0.0	0.0	10.93468338894642	0.0
11	2.0186238233946388E-4	0.0	0.0	11.757474459362074	0.0
12	2.0186238233946388E-4	0.0	0.0	14.170537377848026	0.0
13	2.0186238233946388E-4	0.0	0.0	14.614029031847828	0.0
14	2.0186238233946388E-4	0.0	0.0	14.825378946157247	0.0
15	2.0186238233946388E-4	0.0	0.0	15.320345507653613	0.0
16	2.0186238233946388E-4	0.0	0.0	15.775343317446763	0.0
17	2.0186238233946388E-4	0.0	0.0	16.312902841616758	0.0
18	2.0186238233946388E-4	0.0	0.0	16.941704162604186	0.0
19	2.0186238233946388E-4	0.0	0.0	17.58806751085515	0.0
20	2.0186238233946388E-4	0.0	0.0	17.959090569595084	0.0
21	2.0186238233946388E-4	0.0	0.0	18.289539289484786	0.0
22	2.0186238233946388E-4	0.0	0.0	18.72455272342633	0.0
23	2.0186238233946388E-4	0.0	0.0	19.066911323874063	0.0
24	2.0186238233946388E-4	0.0	0.0	19.360217365413302	0.0
25	2.0186238233946388E-4	0.0	0.0	19.616178866219744	0.0
26	2.0186238233946388E-4	0.0	0.0	19.877792513731688	0.0
27	2.0186238233946388E-4	0.0	0.0	20.149701142742945	0.0
28	2.0186238233946388E-4	0.0	0.0	20.397991873020487	0.0
29	2.0186238233946388E-4	0.0	0.0	20.662633456267525	0.0
30	2.0186238233946388E-4	0.0	0.0	20.98460395609897	0.0
31	2.0186238233946388E-4	0.0	0.0	21.27044108949165	0.0
32	2.0186238233946388E-4	0.0	0.0	21.553654011913917	0.0
33	2.0186238233946388E-4	0.0	0.0	21.84696005345316	0.0
34	2.0186238233946388E-4	0.0	0.0	22.13663257211029	0.0
35	2.0186238233946388E-4	0.0	0.0	22.427919989826137	0.0
36	2.0186238233946388E-4	0.0	0.0	22.706288215072256	0.0
37	2.0186238233946388E-4	0.0	0.0	22.986271339377094	0.0
38	2.0186238233946388E-4	0.0	0.0	23.263024665564497	0.0
39	2.0186238233946388E-4	0.0	0.0	23.551284147545253	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	30	2.1629912E-6	45.000004	1
GCGCATG	20	7.029396E-4	45.0	1
CCGTCGG	25	3.8875813E-5	45.0	2
CACCCGT	20	7.029396E-4	45.0	16
TAGTACG	20	7.029396E-4	45.0	1
TGCGTAG	20	7.029396E-4	45.0	1
CGTTTTT	9070	0.0	43.83407	1
ACGGGTA	95	0.0	42.63158	5
AACACGT	70	0.0	41.785713	41
ACGGGCT	120	0.0	41.250004	5
TACGGCT	975	0.0	39.692307	7
TCGAGCG	40	3.4529148E-7	39.375	1
TCGTTCA	75	0.0	39.0	16
GTTTTTT	10400	0.0	38.834137	2
GTTGATC	105	0.0	38.57143	16
CACGGGC	105	0.0	38.57143	4
GTGCACG	70	0.0	38.571426	1
CGTATGG	35	6.2410927E-6	38.571426	2
CGAGACA	70	0.0	38.571426	22
ACGGCTG	1015	0.0	38.128082	8
>>END_MODULE