FastQCFastQC Report
Sat 18 Jun 2016
SRR3552266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549396
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219503.995296653051715No Hit
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC172903.1470924433377747No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG146452.665654646193274No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC105261.9159222127572824No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC43710.7956009872660158RNA PCR Primer, Index 27 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT40230.7322586986436014RNA PCR Primer, Index 27 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT23370.4253762313522486No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC19410.35329707533363913No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA12560.22861469686710498No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT10910.19858171519268436No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT6180.11248716772601183RNA PCR Primer, Index 38 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT207.0300663E-445.00000411
CATACGA207.0300663E-445.00000418
CGTCATA207.0300663E-445.00000439
TCGTCGG253.8881375E-545.02
AATACGG253.8881375E-545.02
TGCGACG302.1634241E-644.9999961
CGTTTTT99200.044.2741971
TACGGCT14750.041.03397
CGTTCAT556.002665E-1140.90909217
CACGACC1050.040.71428727
CGAGACA1000.040.522
TCAAGCG1000.040.517
AACACGT950.040.26315741
CCCTCGT900.040.014
TCGTGTA850.039.70588317
CGTGTAC850.039.70588318
GTTTTTT112300.039.650492
ACGGCTG15500.039.0483868
GAATCTG22250.039.033711
ACACGAC1050.038.5714326