Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552266_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 549396 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 21950 | 3.995296653051715 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC | 17290 | 3.1470924433377747 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG | 14645 | 2.665654646193274 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGC | 10526 | 1.9159222127572824 | No Hit |
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 4371 | 0.7956009872660158 | RNA PCR Primer, Index 27 (95% over 23bp) |
GAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT | 4023 | 0.7322586986436014 | RNA PCR Primer, Index 27 (95% over 21bp) |
GAATGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT | 2337 | 0.4253762313522486 | No Hit |
GAATGATCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC | 1941 | 0.35329707533363913 | No Hit |
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTA | 1256 | 0.22861469686710498 | No Hit |
GAATGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT | 1091 | 0.19858171519268436 | No Hit |
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT | 618 | 0.11248716772601183 | RNA PCR Primer, Index 38 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGATCT | 20 | 7.0300663E-4 | 45.000004 | 11 |
CATACGA | 20 | 7.0300663E-4 | 45.000004 | 18 |
CGTCATA | 20 | 7.0300663E-4 | 45.000004 | 39 |
TCGTCGG | 25 | 3.8881375E-5 | 45.0 | 2 |
AATACGG | 25 | 3.8881375E-5 | 45.0 | 2 |
TGCGACG | 30 | 2.1634241E-6 | 44.999996 | 1 |
CGTTTTT | 9920 | 0.0 | 44.274197 | 1 |
TACGGCT | 1475 | 0.0 | 41.0339 | 7 |
CGTTCAT | 55 | 6.002665E-11 | 40.909092 | 17 |
CACGACC | 105 | 0.0 | 40.714287 | 27 |
CGAGACA | 100 | 0.0 | 40.5 | 22 |
TCAAGCG | 100 | 0.0 | 40.5 | 17 |
AACACGT | 95 | 0.0 | 40.263157 | 41 |
CCCTCGT | 90 | 0.0 | 40.0 | 14 |
TCGTGTA | 85 | 0.0 | 39.705883 | 17 |
CGTGTAC | 85 | 0.0 | 39.705883 | 18 |
GTTTTTT | 11230 | 0.0 | 39.65049 | 2 |
ACGGCTG | 1550 | 0.0 | 39.048386 | 8 |
GAATCTG | 2225 | 0.0 | 39.03371 | 1 |
ACACGAC | 105 | 0.0 | 38.57143 | 26 |