##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	252111
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.926508561704964	25.0	16.0	30.0	16.0	33.0
2	24.794951430124033	28.0	16.0	31.0	16.0	33.0
3	26.073963452606193	30.0	19.0	31.0	16.0	33.0
4	26.88325777137848	28.0	19.0	35.0	19.0	35.0
5	29.54817124203228	32.0	28.0	35.0	19.0	35.0
6	28.755786141818486	33.0	25.0	35.0	15.0	35.0
7	30.49479395980342	32.0	28.0	35.0	23.0	35.0
8	32.13157696411501	35.0	32.0	35.0	26.0	37.0
9	32.96821638087985	35.0	32.0	37.0	26.0	39.0
10	31.903209300665182	35.0	28.0	37.0	17.0	39.0
11	32.66568297297619	35.0	31.0	39.0	18.0	39.0
12	32.70656972524007	35.0	30.0	39.0	22.0	39.0
13	33.158406416221425	35.0	31.0	38.0	25.0	39.0
14	32.868153313421466	36.0	31.0	39.0	18.0	41.0
15	33.693849137879745	36.0	31.0	39.0	25.0	41.0
16	33.86366322770526	36.0	32.0	39.0	23.0	41.0
17	31.855670716470126	34.0	27.0	38.0	18.0	41.0
18	32.643537965419995	35.0	30.0	39.0	18.0	39.0
19	32.54261813248926	36.0	30.0	37.0	18.0	40.0
20	32.75625418962282	35.0	30.0	37.0	25.0	39.0
21	33.36344705308376	35.0	32.0	38.0	25.0	39.0
22	34.02266065344233	35.0	32.0	38.0	27.0	40.0
23	35.01491803213664	36.0	33.0	39.0	29.0	40.0
24	32.89757289447902	35.0	31.0	37.0	24.0	39.0
25	32.04933937828972	35.0	30.0	38.0	19.0	39.0
26	31.91500569193728	34.0	29.0	37.0	21.0	39.0
27	33.336105921598026	35.0	31.0	38.0	24.0	39.0
28	33.72062702539754	35.0	33.0	38.0	24.0	40.0
29	33.94875669843839	36.0	33.0	39.0	24.0	40.0
30	32.38057839602397	35.0	30.0	38.0	20.0	40.0
31	32.601766682136045	35.0	30.0	38.0	22.0	40.0
32	32.58293370777157	35.0	31.0	38.0	21.0	40.0
33	32.92635386793912	35.0	32.0	38.0	20.0	40.0
34	32.1845020645668	35.0	31.0	38.0	16.0	40.0
35	32.03042310728211	35.0	31.0	38.0	15.0	40.0
36	31.399125781897656	35.0	30.0	38.0	10.0	40.0
37	31.660102097885456	35.0	31.0	38.0	10.0	40.0
38	31.20721031609093	35.0	30.0	38.0	10.0	40.0
39	30.977509906350775	35.0	29.0	38.0	10.0	40.0
40	30.714875590513703	35.0	28.0	38.0	10.0	40.0
41	30.47706764084074	35.0	27.0	38.0	10.0	40.0
42	31.285663854413333	35.0	30.0	38.0	10.0	40.0
43	31.334114735176172	35.0	30.0	38.0	10.0	40.0
44	31.508851259960892	35.0	30.0	38.0	10.0	40.0
45	31.821344566480636	35.0	31.0	39.0	10.0	40.0
46	31.632269119554483	35.0	30.0	39.0	10.0	40.0
47	31.618664001174086	35.0	30.0	39.0	10.0	40.0
48	31.44454625145273	35.0	30.0	38.0	10.0	40.0
49	31.50344491117008	35.0	31.0	38.0	10.0	40.0
50	31.561026690624367	35.0	31.0	39.0	10.0	40.0
51	29.958137487059272	34.0	27.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	14.0
10	32.0
11	25.0
12	34.0
13	45.0
14	55.0
15	80.0
16	166.0
17	325.0
18	588.0
19	986.0
20	1490.0
21	2195.0
22	3263.0
23	4488.0
24	6420.0
25	8795.0
26	10139.0
27	10687.0
28	11325.0
29	12822.0
30	15328.0
31	19264.0
32	24560.0
33	30827.0
34	36037.0
35	31531.0
36	15966.0
37	4250.0
38	357.0
39	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.72500604892289	14.841081904399253	18.141215575678967	28.292696470998884
2	43.61412235086926	21.536148759871644	19.261357100642176	15.58837178861692
3	25.61173451376576	20.433459864900776	38.85748737659206	15.097318244741404
4	22.410366862215454	19.089210704808597	43.351936250302444	15.148486182673505
5	24.50745901606832	22.050604693964164	37.51720472331632	15.924731566651198
6	19.769466623828393	27.91786157684512	37.842061631582915	14.470610167743573
7	58.83162575214885	4.528957483013434	32.79944151584024	3.8399752489974652
8	58.251325804903395	9.229664711178806	26.82231239414385	5.696697089773949
9	53.184509997580435	5.170738285913744	28.283970156002713	13.360781560503113
10	31.143424919975725	18.800845659253266	34.90724323809751	15.148486182673505
11	24.537207817191632	20.184363236828222	39.023684012200974	16.25474493377917
12	20.4524990976197	17.21820943949292	43.184153011966956	19.145138450920427
13	19.659197734331308	18.781806426534345	46.717120633371806	14.841875205762541
14	17.99802467960541	21.952235324916405	41.14060870013605	18.909131295342128
15	15.166732114029138	19.275239874499725	48.06533630028043	17.492691711190705
16	16.795379812860208	19.313318339937567	41.67410386694749	22.21719798025473
17	17.015124290491094	19.029316451880323	43.87948165688923	20.076077600739357
18	17.96192946757579	18.919047562383238	43.32893051076708	19.790092459273893
19	17.86712995466283	21.47625450694337	41.03073646132061	19.625879077073193
20	18.55928539413195	20.34183355744097	44.475647631400456	16.62323341702663
21	18.832974364466445	23.17114286960902	41.02478670109594	16.971096064828586
22	16.84853100420053	18.6037102704761	42.231794725339235	22.315963999984135
23	16.852894161698618	23.5792964210209	41.50116416975062	18.06664524752986
24	18.78339302926092	19.843243650614216	40.4230676170417	20.950295703083167
25	16.472109507320187	23.255629464799235	39.768593992328775	20.5036670355518
26	15.757344978997345	20.183569935464934	42.72086501580653	21.33822006973119
27	17.84967732467048	21.269202851125097	40.177937495785585	20.70318232841883
28	15.42772826255102	21.571450670537978	44.16507014767305	18.835750919237952
29	19.26056379927889	18.975371959176712	41.413107718425614	20.350956523118786
30	17.328874979671653	20.941569388086993	41.93708326887759	19.792472363363757
31	20.90745742946559	21.496483691707226	37.78097742660971	19.815081452217477
32	20.362459392886468	22.871274954286008	38.481859181075	18.284406471752522
33	18.924204021244613	20.495337371237273	37.307376512726535	23.27308209479158
34	21.444522452411835	21.996660201260557	38.32438886046226	18.234428485865354
35	19.036059513468274	20.8761220256157	36.54501390260639	23.542804558309633
36	21.268806200443453	23.37779787474565	36.27648139113327	19.076914533677627
37	21.906620496527324	23.22231080754112	34.575643268242956	20.2954254276886
38	20.523102918952365	25.929451709762763	34.448318399435166	19.0991269718497
39	25.914775634541925	20.921736854004784	34.28648492132434	18.877002590128953
40	21.15774400958308	20.719445006366243	39.519497364256225	18.603313619794456
41	23.246903149803064	23.076343356696057	33.82993998675187	19.84681350674901
42	20.40728091991226	22.594809429180003	37.21376695185851	19.78414269904923
43	23.515435661276186	21.07167081166629	34.109578717311024	21.303314809746503
44	21.525835842148894	21.666646834132585	34.68591215774005	22.12160516597848
45	20.251793852707735	21.10578277028769	35.49309629488599	23.14932708211859
46	23.81570022728084	22.296924767265214	34.983796819654835	18.903578185799113
47	18.595777256843217	20.742054095219963	41.35242016413405	19.309748483802768
48	20.711115342051713	22.676916120280353	34.99529968942252	21.616668848245414
49	19.656024528878152	20.178810127285203	39.39137919408515	20.7737861497515
50	20.67660673274867	19.316888196072366	37.23478943798565	22.771715633193317
51	20.049502005069193	19.909484314448793	34.78666143087767	25.254352249604338
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	342.5
2	566.0
3	8420.5
4	16275.0
5	11058.0
6	5841.0
7	5307.5
8	4774.0
9	4546.5
10	4319.0
11	4126.5
12	3934.0
13	3670.0
14	3406.0
15	3134.5
16	2863.0
17	2640.5
18	2418.0
19	2177.5
20	1937.0
21	1906.0
22	1875.0
23	1845.5
24	1816.0
25	1863.5
26	2033.0
27	2155.0
28	2319.5
29	2484.0
30	2831.5
31	3179.0
32	3429.5
33	3680.0
34	3991.0
35	4302.0
36	4582.0
37	4862.0
38	5248.0
39	5634.0
40	6342.5
41	7051.0
42	7877.0
43	8703.0
44	10098.5
45	11494.0
46	20815.0
47	30136.0
48	25807.5
49	21479.0
50	20880.0
51	20281.0
52	17586.0
53	14891.0
54	13133.5
55	11376.0
56	10476.0
57	9576.0
58	8630.0
59	7684.0
60	7494.5
61	7305.0
62	6670.0
63	6035.0
64	5465.5
65	4896.0
66	4317.0
67	3738.0
68	3270.5
69	2803.0
70	2455.0
71	2107.0
72	1870.0
73	1633.0
74	1359.0
75	923.0
76	761.0
77	559.0
78	357.0
79	285.5
80	214.0
81	154.5
82	95.0
83	69.0
84	43.0
85	26.0
86	9.0
87	7.0
88	5.0
89	3.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	252111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.610794285273016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16594549026566	33.37156888444006
2	10.96109281519458	9.998882990712483
3	5.027331903177563	6.879018037188659
4	3.246440950349512	5.922910013827113
5	2.2595859978846287	5.153075605969958
6	1.5287313159168807	4.183598974064374
7	1.0865028898318052	3.4689381858931188
8	0.7279014763660514	2.6560131598782792
9	0.5149651222664459	2.1139171430206796
>10	1.3903936905009688	9.627722049917194
>50	0.053476639187817684	1.6287847838078402
>100	0.026738319593908842	2.060597943353125
>500	0.0029709243993232045	0.7565764040150118
>1k	0.006932156931754143	7.586993348203077
>5k	0.0	0.0
>10k+	9.903081331077348E-4	4.591402475709012
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10165	4.0319541789132565	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG	4494	1.7825481633090186	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC	4257	1.6885419517593443	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	3277	1.2998242837480316	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1691	0.6707363026603361	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGTGTGT	1048	0.41568991436311786	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTA	1018	0.4037903939137919	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT	1012	0.4014104898239268	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT	582	0.23085069671692232	No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	571	0.22648753921883616	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTC	522	0.2070516558182705	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	397	0.15747032061274596	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGT	301	0.11939185517490312	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC	287	0.11383874563188437	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTAT	259	0.10273252654584687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24552677193775757	0.0
2	0.0	0.0	0.0	1.282371653755687	0.0
3	0.0	0.0	0.0	1.577479760898969	0.0
4	0.0	0.0	0.0	2.3882337541797067	0.0
5	0.0	0.0	0.0	4.562672790953191	0.0
6	0.0	0.0	0.0	5.152095703876467	0.0
7	0.0	0.0	0.0	5.94539706716486	0.0
8	0.0	0.0	0.0	6.977085490121415	0.0
9	0.0	0.0	0.0	7.389205548349735	0.0
10	0.0	0.0	0.0	10.076117265807522	0.0
11	0.0	0.0	0.0	11.212918119399788	0.0
12	0.0	0.0	0.0	14.023981500212209	0.0
13	0.0	0.0	0.0	14.58365561201217	0.0
14	0.0	0.0	0.0	14.863294342571328	0.0
15	0.0	0.0	0.0	15.661355514039451	0.0
16	0.0	0.0	0.0	16.304326268984692	0.0
17	0.0	0.0	0.0	17.07144868728457	0.0
18	0.0	0.0	0.0	17.89489550237792	7.933013632883928E-4
19	0.0	0.0	0.0	18.790532741530516	7.933013632883928E-4
20	0.0	0.0	0.0	19.288329346993983	7.933013632883928E-4
21	0.0	0.0	0.0	19.776209685416344	7.933013632883928E-4
22	3.966506816441964E-4	0.0	0.0	20.32993403699164	7.933013632883928E-4
23	3.966506816441964E-4	0.0	0.0	20.811467964507695	7.933013632883928E-4
24	3.966506816441964E-4	0.0	0.0	21.193839221612702	7.933013632883928E-4
25	3.966506816441964E-4	0.0	0.0	21.503226753295177	7.933013632883928E-4
26	3.966506816441964E-4	0.0	0.0	21.8399831820111	7.933013632883928E-4
27	3.966506816441964E-4	0.0	0.0	22.169599898457424	7.933013632883928E-4
28	3.966506816441964E-4	0.0	0.0	22.49723336149553	7.933013632883928E-4
29	3.966506816441964E-4	0.0	0.0	22.814157256129246	7.933013632883928E-4
30	3.966506816441964E-4	0.0	0.0	23.177885931196972	7.933013632883928E-4
31	3.966506816441964E-4	0.0	0.0	23.485686860152867	7.933013632883928E-4
32	3.966506816441964E-4	0.0	0.0	23.820856686142214	7.933013632883928E-4
33	3.966506816441964E-4	0.0	0.0	24.156423162813205	7.933013632883928E-4
34	3.966506816441964E-4	0.0	0.0	24.461844187679237	7.933013632883928E-4
35	3.966506816441964E-4	0.0	0.0	24.795427410942004	7.933013632883928E-4
36	3.966506816441964E-4	0.0	0.0	25.113144606939006	7.933013632883928E-4
37	3.966506816441964E-4	0.0	0.0	25.43006850157272	7.933013632883928E-4
38	3.966506816441964E-4	0.0	0.0	25.73429957439382	7.933013632883928E-4
39	3.966506816441964E-4	0.0	0.0	26.046463660847802	7.933013632883928E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCA	20	7.0228014E-4	45.0	34
TCGTTCA	20	7.0228014E-4	45.0	16
ACACGAG	20	7.0228014E-4	45.0	37
ACACGAC	20	7.0228014E-4	45.0	26
ACGCCCG	20	7.0228014E-4	45.0	21
GTATTAG	20	7.0228014E-4	45.0	1
CAACGAG	25	3.8821167E-5	45.0	14
ACGCATG	20	7.0228014E-4	45.0	1
CACGACC	20	7.0228014E-4	45.0	27
CCGCTTG	25	3.8821167E-5	45.0	39
TAACGGG	25	3.8821167E-5	45.0	3
CGTTCAT	20	7.0228014E-4	45.0	17
GTTACGG	20	7.0228014E-4	45.0	2
TCTACGG	20	7.0228014E-4	45.0	2
TGATTGG	25	3.8821167E-5	45.0	2
TACTGAC	20	7.0228014E-4	45.0	20
AACGAGC	25	3.8821167E-5	45.0	15
CGAATAG	25	3.8821167E-5	45.0	26
ACTGTCG	25	3.8821167E-5	45.0	25
CGAGACA	20	7.0228014E-4	45.0	22
>>END_MODULE