FastQCFastQC Report
Sat 18 Jun 2016
SRR3552264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280994
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109353.891542168160174No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG63182.2484465860481007No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC59442.1153476586688686No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC52331.8623173448543386No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC19200.683288611144722TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT13020.4633550894325145No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT10640.37865577200936673No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTA10630.37829989252439555No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTC9110.3242062108087717No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT6300.22420407553186192TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC4100.14591058883819583TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGT3180.11316967622084456No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGTGTGT3160.11245791725090215No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTT3140.11174615828095974No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC2900.10320505064165071No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTAT2860.10178153270176586No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAA207.02418E-445.025
TTGTCCG207.02418E-445.025
ATTCGGC207.02418E-445.017
ATCCGTC207.02418E-445.06
GCGATTA253.8832586E-545.09
CGTCGCG207.02418E-445.015
CCCGATC207.02418E-445.041
ACAACGA302.159628E-644.99999613
TACGAAT302.159628E-644.99999612
CGGGCAC302.159628E-644.9999966
CGAATAT302.159628E-644.99999614
CGTAAGG302.159628E-644.9999962
GCTACGA302.159628E-644.99999610
CACAACG302.159628E-644.99999612
CGTTTTT51850.043.6113781
AACGGGA556.002665E-1140.9090924
TAGGCAG451.9193976E-840.01
AATGATC1700.039.7058832
CGTTCGG403.4458026E-739.37545
ACGGGCG750.039.05
Produced by FastQC (version 0.11.5)