##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	280994
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.04092614077169	28.0	16.0	30.0	16.0	33.0
2	26.23411531918831	30.0	25.0	31.0	16.0	33.0
3	27.16673665629871	30.0	25.0	31.0	16.0	33.0
4	27.94758251065859	30.0	19.0	35.0	19.0	35.0
5	30.01274404435682	32.0	28.0	35.0	19.0	35.0
6	30.593742215136267	33.0	28.0	35.0	17.0	35.0
7	31.99730243350392	33.0	31.0	35.0	28.0	35.0
8	33.119589742129726	35.0	33.0	35.0	28.0	37.0
9	34.06891606226468	35.0	32.0	37.0	27.0	39.0
10	34.26326184900745	35.0	32.0	39.0	27.0	39.0
11	34.756788401175825	37.0	34.0	39.0	27.0	39.0
12	32.74480949771169	35.0	30.0	37.0	25.0	39.0
13	33.8164622732158	35.0	32.0	37.0	27.0	39.0
14	35.02989031794273	37.0	33.0	39.0	27.0	41.0
15	35.37340654960604	37.0	33.0	39.0	27.0	41.0
16	35.83668334555186	37.0	34.0	40.0	30.0	41.0
17	34.13934105354563	37.0	31.0	39.0	24.0	41.0
18	34.26748969728891	37.0	32.0	39.0	26.0	40.0
19	33.98219179057204	37.0	32.0	38.0	25.0	40.0
20	34.22102607173107	35.0	32.0	38.0	27.0	40.0
21	35.02236702563044	36.0	34.0	39.0	30.0	40.0
22	35.67645928382812	37.0	34.0	39.0	30.0	40.0
23	36.019669459134356	37.0	35.0	40.0	31.0	40.0
24	35.787162715218116	37.0	35.0	40.0	31.0	40.0
25	34.68788657409055	36.0	33.0	39.0	27.0	40.0
26	34.91670640654249	36.0	33.0	39.0	27.0	40.0
27	35.6230524495185	37.0	34.0	39.0	30.0	40.0
28	35.48208502672655	37.0	34.0	40.0	30.0	40.0
29	35.645693502352366	37.0	35.0	40.0	30.0	41.0
30	35.1946767546638	37.0	34.0	40.0	27.0	41.0
31	34.80721652419625	37.0	34.0	40.0	25.0	41.0
32	34.50600368691146	37.0	34.0	40.0	25.0	40.0
33	34.1292233997879	37.0	33.0	40.0	21.0	41.0
34	32.80503142415852	36.0	32.0	39.0	18.0	40.0
35	32.78230851904311	36.0	32.0	39.0	15.0	40.0
36	32.46547257236809	36.0	32.0	39.0	14.0	40.0
37	32.47614183932753	36.0	32.0	39.0	12.0	40.0
38	32.24019374079162	36.0	31.0	39.0	10.0	40.0
39	31.889915798913854	35.0	31.0	39.0	10.0	40.0
40	31.633540217940595	35.0	30.0	39.0	10.0	40.0
41	31.58801611422308	35.0	30.0	39.0	10.0	40.0
42	31.653875171711853	35.0	30.0	39.0	10.0	40.0
43	31.786942069937435	36.0	31.0	39.0	10.0	40.0
44	31.45932653366264	35.0	30.0	39.0	10.0	40.0
45	31.643334021367004	35.0	30.0	39.0	10.0	40.0
46	31.304344576752527	35.0	30.0	38.0	10.0	40.0
47	31.35943116223122	35.0	30.0	39.0	10.0	40.0
48	31.3856523626839	35.0	30.0	39.0	10.0	40.0
49	31.050296447610982	35.0	29.0	38.0	10.0	40.0
50	31.059499491092335	35.0	29.0	38.0	10.0	40.0
51	27.795109504117526	31.0	23.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	10.0
10	16.0
11	25.0
12	16.0
13	27.0
14	36.0
15	78.0
16	136.0
17	290.0
18	528.0
19	807.0
20	1297.0
21	1823.0
22	2587.0
23	3697.0
24	5516.0
25	8102.0
26	9958.0
27	10500.0
28	10277.0
29	10419.0
30	11932.0
31	15104.0
32	19175.0
33	25379.0
34	33826.0
35	43470.0
36	37679.0
37	21886.0
38	6036.0
39	351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68058392705894	14.619885122102252	17.834188630362213	26.865342320476593
2	41.50764785013203	27.044705580902082	18.749510665708165	12.69813590325772
3	24.93967842729738	25.22509377424429	37.37268411425155	12.46254368420678
4	22.24709424400521	18.42138978056471	44.80202424251052	14.529491732919563
5	25.532929528744386	21.880182494999893	36.11678541178816	16.470102564467567
6	18.75662825540759	32.169014285002525	37.40435738841399	11.670000071175897
7	58.73719723552816	4.845655067367987	32.42489163469683	3.9922560624070265
8	57.10762507384499	10.694890282354784	26.79487818245229	5.402606461347929
9	52.523897307415815	5.451006071304013	27.476743275657135	14.548353345623038
10	30.952974084855907	18.711431560816244	34.73027893834032	15.60531541598753
11	24.288774849285037	20.061282447312042	38.56452450942013	17.08541819398279
12	21.0581008847164	17.198587870203635	42.69948824530061	19.043822999779355
13	18.973714741240027	18.82673651394692	47.64443368897556	14.555115055837492
14	18.065866175078472	23.44462871093333	39.74462088158466	18.74488423240354
15	14.962952945614497	19.414649423119354	49.108877769632095	16.51351986163406
16	16.454443867128834	20.46129098841968	40.25673146045823	22.827533683993252
17	16.654092258197682	19.15485739909037	43.775667807853544	20.415382534858395
18	17.60927279586041	20.155946390314384	42.625465312426606	19.609315501398605
19	17.321722172003675	22.400122422542832	39.99765119539919	20.280504210054307
20	17.985437411474976	21.515406022904404	43.804849925621184	16.694306639999432
21	18.158038961686014	25.134344505576635	40.31011338320391	16.397503149533442
22	16.457290903008605	18.81570424991281	40.794465362249724	23.932539484828858
23	16.43166758009068	24.98736627828352	40.84713552602547	17.733830615600333
24	19.343473526125113	19.779781774699813	38.75242887748493	22.124315821690143
25	16.07614397460444	26.310526203406475	38.082663686769116	19.530666135219967
26	15.734855548517048	19.915727737958818	41.75000177939743	22.599414934126706
27	18.7157021146359	21.938902610020143	38.256332875435064	21.089062399908894
28	15.13982504964519	21.192623330035516	43.94115176836516	19.726399851954135
29	19.7082499982206	18.954853128536552	40.444991708008	20.891905165234846
30	17.032036271237107	21.971643522637493	41.01012833014228	19.986191875983117
31	21.162729453297935	22.09655722186239	36.49544118379752	20.245272141042157
32	21.860609123326476	22.69123184124928	37.375531150131316	18.072627885292924
33	18.70075517626711	19.948112771091196	37.154885869449174	24.196246183192525
34	22.622547100649836	21.14991779183897	38.01255542822978	18.21497967928141
35	19.172651373338933	20.103632106023618	36.277642938995136	24.446073581642313
36	22.000469760920165	23.662071076250736	35.781191057460305	18.556268105368797
37	22.075560332249086	22.30296732314569	34.679032292504466	20.942440052100757
38	19.747040862082464	25.801974419382624	34.13418080101355	20.31680391752137
39	27.60272461333694	20.120002562332292	33.28647586781213	18.990796956518643
40	20.594745795283885	20.067688278041523	40.461362164316675	18.876203762357914
41	23.949265820622504	23.241065645529797	32.365103881221664	20.444564652626035
42	20.230681082158338	21.959899499633444	38.343167469768034	19.46625194844018
43	24.552837427133674	20.550616739147458	32.707104066278994	22.189441767439874
44	21.70010747560446	21.098315266518146	34.623515092848955	22.578062165028435
45	20.153455233919583	20.138864175035767	34.995409154643866	24.712271436400776
46	24.658889513655097	22.291579179626613	33.877235812864335	19.172295493853962
47	18.60395595635494	20.00256233229179	41.86993316583273	19.523548545520544
48	20.804358811931927	22.565606383054444	34.156601208566734	22.4734335964469
49	19.518566232730947	19.216068670505422	39.850317088621104	21.415048008142524
50	20.685495063951542	18.711787440301215	37.194032612796	23.408684882951235
51	20.28192772799419	19.21927158587016	33.84449490024698	26.654305785888667
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	363.5
2	597.0
3	8573.5
4	16550.0
5	11256.5
6	5963.0
7	5491.0
8	5019.0
9	4778.5
10	4538.0
11	4393.5
12	4249.0
13	3999.5
14	3750.0
15	3527.5
16	3305.0
17	2959.0
18	2613.0
19	2431.0
20	2249.0
21	2228.0
22	2207.0
23	2116.5
24	2026.0
25	2113.0
26	2361.5
27	2523.0
28	2642.5
29	2762.0
30	3137.5
31	3513.0
32	3744.0
33	3975.0
34	4307.0
35	4639.0
36	4989.5
37	5340.0
38	5847.0
39	6354.0
40	7212.0
41	8070.0
42	8974.5
43	9879.0
44	11620.5
45	13362.0
46	26716.5
47	40071.0
48	31818.5
49	23566.0
50	22904.5
51	22243.0
52	19293.0
53	16343.0
54	14298.0
55	12253.0
56	11136.5
57	10020.0
58	9198.5
59	8377.0
60	8163.0
61	7949.0
62	7204.0
63	6459.0
64	5784.0
65	5109.0
66	4535.5
67	3962.0
68	3402.0
69	2842.0
70	2510.0
71	2178.0
72	1867.5
73	1557.0
74	1300.0
75	804.5
76	566.0
77	426.5
78	287.0
79	229.5
80	172.0
81	138.0
82	104.0
83	71.0
84	38.0
85	28.0
86	18.0
87	14.5
88	11.0
89	9.5
90	8.0
91	5.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	280994.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.818576414923605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.72262664223545	37.64403937675237
2	7.760294299644349	7.421724519396787
3	3.626967968078075	5.20309334808065
4	2.418502824113064	4.625974484182364
5	1.860281252394747	4.447800061044399
6	1.4847898241773378	4.260032140051501
7	1.0762171341579023	3.602421988814582
8	0.8092655385139097	3.0958340794713353
9	0.5567139098365549	2.3959139974893167
>10	1.6064175594612458	11.029838612287488
>50	0.049451164688139676	1.6008196122715466
>100	0.02097928198890774	2.017863933821251
>500	0.0014985201420648386	0.5521179549038615
>1k	0.002997040284129677	1.91646913743073
>5k	0.002247780213097258	6.2682048157320285
>10k+	7.492600710324193E-4	3.9178519382697763
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10935	3.891542168160174	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG	6318	2.2484465860481007	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC	5944	2.1153476586688686	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	5233	1.8623173448543386	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1920	0.683288611144722	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT	1302	0.4633550894325145	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCT	1064	0.37865577200936673	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTA	1063	0.37829989252439555	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTC	911	0.3242062108087717	No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGCT	630	0.22420407553186192	TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	410	0.14591058883819583	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGT	318	0.11316967622084456	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGTGTGT	316	0.11245791725090215	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTT	314	0.11174615828095974	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCC	290	0.10320505064165071	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTAT	286	0.10178153270176586	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2743830829128024	0.0
2	0.0	0.0	0.0	1.4018092913015936	0.0
3	3.558794849712094E-4	0.0	0.0	1.7025274561022656	0.0
4	3.558794849712094E-4	0.0	0.0	2.5996996377146844	0.0
5	3.558794849712094E-4	0.0	0.0	5.832864758678121	0.0
6	3.558794849712094E-4	0.0	0.0	6.517576887762728	0.0
7	3.558794849712094E-4	0.0	0.0	7.564930212032997	0.0
8	3.558794849712094E-4	0.0	0.0	8.823320070891194	0.0
9	3.558794849712094E-4	0.0	0.0	9.277778173199428	0.0
10	3.558794849712094E-4	0.0	0.0	12.725538623600505	0.0
11	3.558794849712094E-4	0.0	0.0	13.879655793362137	0.0
12	3.558794849712094E-4	0.0	0.0	17.307131113119855	0.0
13	3.558794849712094E-4	0.0	0.0	17.871555976284192	0.0
14	3.558794849712094E-4	0.0	0.0	18.173697659024747	0.0
15	3.558794849712094E-4	0.0	0.0	18.955920766991465	0.0
16	3.558794849712094E-4	0.0	0.0	19.674441447148336	0.0
17	3.558794849712094E-4	0.0	0.0	20.415026655373424	0.0
18	3.558794849712094E-4	0.0	0.0	21.234261229777147	0.0010676384549136281
19	3.558794849712094E-4	0.0	0.0	21.801889008306226	0.0010676384549136281
20	3.558794849712094E-4	0.0	0.0	22.25599123112949	0.0010676384549136281
21	3.558794849712094E-4	0.0	0.0	22.775219399702486	0.0010676384549136281
22	3.558794849712094E-4	0.0	0.0	23.30725922973444	0.0010676384549136281
23	3.558794849712094E-4	0.0	0.0	23.80940518302882	0.0010676384549136281
24	3.558794849712094E-4	0.0	0.0	24.191263870402928	0.0010676384549136281
25	3.558794849712094E-4	0.0	0.0	24.511555406877015	0.0010676384549136281
26	3.558794849712094E-4	0.0	0.0	24.832202822836074	0.0010676384549136281
27	3.558794849712094E-4	0.0	0.0	25.150714961885306	0.0010676384549136281
28	3.558794849712094E-4	0.0	0.0	25.48986811106287	0.0010676384549136281
29	3.558794849712094E-4	0.0	0.0	25.811939044961814	0.0010676384549136281
30	3.558794849712094E-4	0.0	0.0	26.206609393794885	0.0010676384549136281
31	3.558794849712094E-4	0.0	0.0	26.525121532844118	0.0010676384549136281
32	3.558794849712094E-4	0.0	0.0	26.87068051275116	0.0010676384549136281
33	3.558794849712094E-4	0.0	0.0	27.21481597471832	0.0010676384549136281
34	3.558794849712094E-4	0.0	0.0	27.53439575222247	0.0010676384549136281
35	3.558794849712094E-4	0.0	0.0	27.877819455219683	0.0010676384549136281
36	3.558794849712094E-4	0.0	0.0	28.168572994441163	0.0010676384549136281
37	3.558794849712094E-4	0.0	0.0	28.466800002847037	0.0010676384549136281
38	3.558794849712094E-4	0.0	0.0	28.786379780351183	0.0010676384549136281
39	3.558794849712094E-4	0.0	0.0	29.087097945151854	0.0010676384549136281
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAA	20	7.02418E-4	45.0	25
TTGTCCG	20	7.02418E-4	45.0	25
ATTCGGC	20	7.02418E-4	45.0	17
ATCCGTC	20	7.02418E-4	45.0	6
GCGATTA	25	3.8832586E-5	45.0	9
CGTCGCG	20	7.02418E-4	45.0	15
CCCGATC	20	7.02418E-4	45.0	41
ACAACGA	30	2.159628E-6	44.999996	13
TACGAAT	30	2.159628E-6	44.999996	12
CGGGCAC	30	2.159628E-6	44.999996	6
CGAATAT	30	2.159628E-6	44.999996	14
CGTAAGG	30	2.159628E-6	44.999996	2
GCTACGA	30	2.159628E-6	44.999996	10
CACAACG	30	2.159628E-6	44.999996	12
CGTTTTT	5185	0.0	43.611378	1
AACGGGA	55	6.002665E-11	40.909092	4
TAGGCAG	45	1.9193976E-8	40.0	1
AATGATC	170	0.0	39.705883	2
CGTTCGG	40	3.4458026E-7	39.375	45
ACGGGCG	75	0.0	39.0	5
>>END_MODULE