FastQCFastQC Report
Sat 18 Jun 2016
SRR3552263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367465
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135003.673819275305131No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG105632.8745594818554148No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC87982.3942416284544104No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC86052.3417196195556036No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC43841.1930387928101998No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT30810.8384471990529709No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA21580.5872668145265536No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT19840.5399153660892875No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC13930.3790837222592628No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT13910.37853945273699535No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC9700.26397071829970203No Hit
CTGAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC9270.2522689235709523No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT8600.23403589457499352No Hit
CTGAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT7050.19185500659926796No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC6600.17960894234825084No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGT5600.1523954662348795No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTAT4900.13334603295551958No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT4850.13198535914985102No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATG4820.13116895486644986No Hit
GAATCTTTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC4150.11293592587049107No Hit
GAACTGTCCCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT4140.11266379110935737No Hit
TCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC4110.11184738682595621No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGCCTAA3750.10205053542514254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG253.885609E-545.04
ACAACTA253.885609E-545.015
TACGGTG207.027015E-445.07
CTAACGG207.027015E-445.02
CGTTTTC253.885609E-545.01
TGCGTTG207.027015E-445.01
GTGCCTC302.1614578E-644.99999621
CGTTTTT69850.044.2913361
GTTGATC1050.040.71428716
CGAAAGG451.9221261E-840.0000042
ACGGGCT900.040.0000045
TACCTGG1250.039.60000210
ACGGGAA800.039.3755
CGTCTTT1200.039.3749961
GTTTTTT80000.039.0093772
TAGCATA1050.038.5714330
TAGGGTA1050.038.571435
AGTAGCA1050.038.5714328
TCGTTCA356.2366944E-638.57142616
CATATTA700.038.57142628
Produced by FastQC (version 0.11.5)